• 한국작물학회지
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• 제33권2호
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• pp.195-200
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• 1988

벼를 보통기에 동일하게 이앙하여 추앙후 20일, 유전형성기, 감수분열기 및 출수기의 생육시기별로 포장상태에서 3일간씩 관수처리한 경우와 품종, 이앙기 및 재배조건이 다양한 농가포장에서 태풍 'Thelma 호'('87년 7월 16~18일)에 의해 3일간 관수된 경우에 생육단계별 피해상태와 피해주의 고립분얼이삭의 발생에 의한 수량 보상력을 검토한 결과는 다음과 같다. 1. 보통기 이앙된 벼의 생육시기별 관수처리에서는 생육이 진전될수록 관수된 수온이 높아져 피해가 컸으나 고온기에 여러 생육단계를 갖는 농가포장이 일시에 관수되면 어린 생육단계일수록 피해가 컸다. 2. 생식생장 관수 피해수의 수량보상은 고립절분얼이삭에 의존하였고 고립절분얼이삭이 9월 15일 출수되어 등숙적산온도가 69$0^{\circ}C$, 등숙일조시간이 210시간이 확보되었을 때 쌀 230kg/10a을 보상하였다.0a을 보상하였다.

• 한국작물학회지
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• 제50권1호
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• pp.16-21
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• 2005

This study was conducted to establish the elaborate nitrogen fertilization method to enhance N use efficiency in direct-seeded rice on flooded paddy. The nitrogen uptake by rice plants was insignificant until 25 days after seeding, and increased gradually thereafter. During this early growth stage, rice plants absorbed only the $4\%$ of basal applied N, while the $45\%$ of N fertilizer remained in the paddy soil. The absorption of basal N by rice plants was almost completed at 46 days after application. Nitrogen top-dressed at 5-leaf stage was well matched to crop nutrient demand, so it could be absorbed so actively in 8days after application. As a result, we could cut down the amount of N fertilizer to $36\%$ of the basal N level without significant difference in yield. Plant recoveries of fertilizer $^{15}N$ applied with different application timings were $7.8\%$ for basal, $9.4\%$ for 5-leaf stage, $17.1\%$ for tillering stage, and $23.4\%$ for panicle initiation stage, respectively. When urea was applied with nitrogen fertilization practice based on basal incorporation (BN), plant recovery of $^{15}N$ at harvest was $31.0\%$, which was originated from $13.7\%$ for grain, and $21.3\%$ of the fertilizer $^{15}N$ remained in the soil, and the rest could be uncounted. Plant recovery of fertilizer $^{15}N$ applied with nitrogen fertilization practice based on topdressing at 5-leaf stage (TN), where N rate was reduced by $18\%$ compared with BN, was $35.1\%$ (grain $15.6\%$), and $19.9\%$ of the fertilizer $^{15}N$ remained in the soil, and the rest could be uncounted. TN showed a higher $^{15}N$ recovery than BN because it was to apply N fertilizer at a time to well meet the demand of rice plant direct-seeded on flooded paddy. We concluded that TN would be the nitrogen fertilization method to enhance N use efficiency in direct-seeded rice on flooded paddy.

• Journal of Photoscience
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• 제3권1호
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• pp.9-14
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• 1996

For three cultivars of chilling-sensitive cucumber plants, chilling sensitivity was evaluated in terms of photosynthetic activity using Chl fluorescence techniques. Low-temperature treatment caused a decrease in photosynthetic activities of cucumber leaves, measured as CO$_2$ exchange, as well as the decrease in the stomatal conductance. FR of the three cultivars decreased after chilling for 24 h in light and the extent of decline of F$_R$ was the greatest in 'Chosaeng' cultivar. When these plants were recovered from light-chilling, 'Chosaeng' and 'Samchuk' cultivars did not fully restore the original value of F$_R$ after 24 h of recovery, in contrast to 'Ilmi' cultivar which showed a rather efficient recovery. The results of FR study showed that 'Chosaeng' was most susceptible, whereas Ilmi was most resistant, to chilling among the three cultivars of cucumber plants. When quenching coefficients for chlorophyll fluorescence was analyzed after chilling the cucumber plants for 24 h in light, 'Chosaeng' elicited more rapid declines in the coefficients for photochemical quenching (qQ), non-photochemical quenching (qNP) and energy-dependent quenching (qE) than 'Ilmi' and 'Samchuk'. The implications of these observations are discussed in relation to the growth habits of the respective cultivars in the field. The results showed that measurement of chlorophyll fluorescence was an effective means of screening chilling tolerance of cucumber plants. Furthermore, the study on the chlorophyll fluorescence induction and fluorescence quenching charactersitics showed that low temperature could accelerate inhibition of photosynthesis in chilling-sensitive plants, by limiting Calvin cycle activity and disrupting, in part, the energy dissipation mechanims of the photosystem II.

• 대한한의학회지
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• 제24권4호
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• pp.71-81
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• 2003

So-Hap-Hyang-Won, a traditional oriental medicine used in the treatment of stroke patients, was examined for its ability to reverse the cell damage caused by lipid peroxidation products and oxidative stress in bovine aortic endothelial cells (BAEC). The effects of herbal medicine on cell proliferation and recovery of oxidative damaged situation were studied in BAEC, which was considered an appropriate in vitro model for stroke resulting from various vascular diseases prevalent in advanced age. In a clinical study of stroke patients, So-Hap-Hyang-Won appeared to improve considerably arm and leg movements as well as consciousness disturbance condition, compared with other traditional medicines used for stroke. When BAEC were treated with extracts of the lyophilized herbal medicines, only that of So-Hap-Hyang-Won stimulated cell proliferation and showed no toxicity even at high concentrations. In studies of BAEC treated with extracts of the lyophilized material of the 14 components of So-Hap-Hyang-Won, only the extract of Foeniculi Fructus stimulated cell growth at all concentrations tested. Moreover, when cells were treated with Foeniculi Fructus (10 and 100 mg/ml) extract after prior exposure to t-BHP ($l0\mu\textrm{M}$) or HNE ($0.2\mu\textrm{M}$), lipid peroxidation products which are known to be involved in aging and vascular diseases, or after the exposure to SIN-l ($500\mu\textrm{M}$), which generates nitric oxide (NO) and other reactive oxygen species, there was substantial recovery from the oxidative damage, presumably due to the radical-scavenging effect of Foeniculi Fructus extract. Foeniculi Fructus not only showed stimulatory effects on cell growth and cell damage repair in BAEC, but also appeared to show the most anti-aging activity among all the herbal components of So-Hap-Hyang-Won.

• 한방재활의학과학회지
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• 제19권2호
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• pp.1-25
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• 2009

Objectives : The purpose of this study was to investigate the effects of Yanghyuljanggeungunbo-tang(Yangxuezhuangjinjianbu-tang, YGKT) and electrical acupuncture treatment in spinal cord injury(SCI)-induced rats. Methods : The subjects were divided into 5 groups ; Normal, Control-no treatment after SCI, Experimental I(Exp. I)-taken with YGKT 500 mg/kg $0.5m{\ell}$ daily after SCI. Experimental II(Exp. II)-taken with electrical acupuncture after SCI and Experimental III(Exp. III)-taken with YGKT 500 mg/kg $0.5m{\ell}$ and electrical acupuncture after SCI. After each operation, the present author observed cytological changes, the motor behavior recovery and nerve regeneration by analysis of the motor behavior tests, EMG, hematological(AST, ALT, WBC), histological and immunological changes. Rats were tested by Motor behavior test at 1st, 3rd, 7th, 14th and 21st day. Results : 1. All the experimental groups were improved compared with control group in the motor behavior tests including Tarlov test, Basso-Beattle-Bresnahan locomotor rating scale, modified inclined plane test, open field test, grid walk test and narrow beam test. Especially Exp. III was significantly improved among other groups. 2. In EMG test, H and M wave were significantly increased in Exp. III. 3. All the experimental groups were significantly decreased compared with control group in AST, ALT and WBC. 4. NGF, BDNF and Trk B of spinal cord gray matter in all the experimental groups were increased compared with control group. Especially, Exp. III was more effective. 5. In histological observations, muscle contraction and denaturation of gastrocnemius muscle of all the experimental groups were inhibited. Especially, those of Exp. III was more effective. On the observations of liver and kidney, cell atrophy and apoptosis of all the experimental groups were decreased compared with control group. Especially, those of Exp. III was more effective. Conclusions : It can be suggested that YGKT and electrical acupuncture may improve motor behavior, EMG, hematological, histological and immunological findings in SCI-induced rats. Especially, combination of these two treatments will be somewhat better in spinal nerve recovery and motor function improvement.

• Journal of Korean Neurosurgical Society
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• 제64권5호
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• pp.705-715
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• 2021

Objective : Through our previous clinical trials, the demonstrated therapeutic effects of MSC in chronic spinal cord injury (SCI) were found to be not sufficient. Therefore, the need to develop stem cell agent with enhanced efficacy is increased. We transplanted enhanced Wnt3-asecreting human mesenchymal stem cells (hMSC) into injured spines at 6 weeks after SCI to improve axonal regeneration in a rat model of chronic SCI. We hypothesized that enhanced Wnt3a protein expression could augment neuro-regeneration after SCI. Methods : Thirty-six Sprague-Dawley rats were injured using an Infinite Horizon (IH) impactor at the T9-10 vertebrae and separated into five groups : 1) phosphate-buffered saline injection (injury only group, n=7); 2) hMSC transplantation (MSC, n=7); 3) hMSC transfected with pLenti vector (without Wnt3a gene) transplantation (pLenti-MSC, n=7); 4) hMSC transfected with Wnt3a gene transplantation (Wnt3a-MSC, n=7); and 5) hMSC transfected with enhanced Wnt3a gene (1.7 fold Wnt3a mRNA expression) transplantation (1.7 Wnt3a-MSC, n=8). Six weeks after SCI, each 5×10⁵ cells/15 µL at 2 points were injected using stereotactic and microsyringe pump. To evaluate functional recovery from SCI, rats underwent Basso-Beattie-Bresnahan (BBB) locomotor test on the first, second, and third days post-injury and then weekly for 14 weeks. Axonal regeneration was assessed using growth-associated protein 43 (GAP43), microtubule-associated protein 2 (MAP2), and neurofilament (NF) immunostaining. Results : Fourteen weeks after injury (8 weeks after transplantation), BBB score of the 1.7 Wnt3a-MSC group (15.0±0.28) was significantly higher than that of the injury only (10.0±0.48), MSC (12.57±0.48), pLenti-MSC (12.42±0.48), and Wnt3a-MSC (13.71±0.61) groups (p<0.05). Immunostaining revealed increased expression of axonal regeneration markers GAP43, MAP2, and NF in the Wnt3a-MSC and 1.7 Wnt3a-MSC groups. Conclusion : Our results showed that enhanced gene expression of Wnt3a in hMSC can potentiate axonal regeneration and improve functional recovery in a rat model of chronic SCI.

• 한국환경생태학회지
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• 제35권2호
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• pp.126-134
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• 2021

식재된 수목이 활착되기 위해 소요되는 시간은 수종별로 차이가 있기 때문에 개별 수종에 대한 생장량 변동 추이 분석을 통해 관리 기간을 설정할 필요가 있다. 이에 본 연구는 수변생태벨트 조성 지역에 식재된 소나무의 연간 생장량 변화 분석 자료를 바탕으로 관리 기간을 제안하고자 하였다. 생장량을 분석한 결과, 식재 전에는 평균 0.6cm, 식재 후 1~2년차 0.3cm, 3~4년차 0.5cm, 5년차 이후에는 약 0.7cm 내외였다. 식재된 소나무의 생장량은 1~2년차 생장 불량, 3~4년차 회복 단계를 거쳐, 5년차부터 정상 생장 단계가 확인되었기 때문에 활엽수와는 달리 최소 4년은 집중관리가 필요한 기간으로 판단되었다. 수목별 활착에 필요한 기간은 차이가 있을 수 있기 때문에 생장량 연구를 통해 수종별 관리 기간을 설정해야 할 것이다.

• 혜화의학회지
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• 제14권2호
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• pp.107-112
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• 2005

Peripheral axons in vertebrate animals can regenerate after nerve injury and accomplish its functional recovery. Numerous studies have revealed that diverse molecular factors are induced during axonal regeneration and their potential roles in axonal regeneration have been studied. Examples is N-CAM, L1, P0, nerve growth factors, GAP-43 and so forth. However, most of the studies on axonal regeneration have been primarily focused on axon fiber regrowth and elucidating molecular factors, and relatively less is known about functional recovery. Also, specific drugs or drug components used in the oriental medicine in relation to nerve fiber regeneration have not been known. And thus, in the present, a study on the effect of Samul-tang components and Samul-tang extracts to regeneration of peripheral axon fiber is underway by immunofluorescence staining. Therefore, this prior application of Samul-tang with documents consideration is reported with a plea for further investigation.

• 동의신경정신과학회지
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• 제14권2호
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• pp.191-198
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• 2003

Dissociative amnesia is a psychiatric disorder charicterized by a sudden loss of memory, but which has no organic disease or explanation, It usually occurs after severe psychosocial stress or traumatic experience. As for medical treatment, medicine, psychotherapy and hypnosis are used. This case describes a 35-years-old woman who developed amnesia and depression following severe troublesome events. So she suited without expression and appealed against headache, dizziness, chest discomfort and weakness. Psychoanalytic psychotherapy was enforced for three days with hypnosis. She expressed the felling over the incident which she dose not like, and canceled in the past. She became reconciled with the family and left the husband by the heart. Then she recovered memory completely and body condition also improved. Recovery and growth of self were helped using supportive expression in order to prevent a recurrence. The prognosis is good with complete recovery, and there was no relapse.

• 재활복지
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• 제20권1호
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• pp.87-111
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• 2016

본 연구는 마약중독에서 탈출하여 회복의 길을 걷고 있는 회복자 5명에 대한 질적 사례연구이다. 마약 중독자들의 회복과 그 내용에 대한 이해는 사회적 실천적 효용성이 있음에도 불구하고 개인들의 지극히 사적이고 민감한 주제를 다루기 때문에 질적연구 방법이 적합하다. 연구자들은 질적연구 방법 중 질적 사례연구방법으로 접근하였으며, 심층면담을 수행하여 자료를 구성하였다. 자료는 Creswell(2007)이 제안한 사례 내 사례 간 주제분석 방법으로 분석하였다. 사례 내 분석에서는 연구참여자들의 개별적인 사례를 기술하고 그들의 경험과 중독, 탈중독 과정의 주요 문제점들을 파악하였다. 사례 간 분석에서는 사례 내 분석을 토대로 마약중독 회복경험 전체에서 나타나는 유사한 주제들을 결집하여 공통주제로 전환하였다. 5명의 개별사례에서 마약중독 회복과 관련하여 총 19개의 주제를 도출했고, 이를 공통성 차원에서 결집한 결과, 첫째, 현실감각의 회복, 둘째, 자기실존 양식 떠안기, 셋째, 사회적 관계 속에서 자기 재구성, 넷째, 자기애의 4가지 공통주제를 발굴했다. 연구결과와 논의에 근거하여 마약중독자의 회복과 재활에 기여할 수 있는 실천차원에서의 제언을 하였다.