• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.4
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• pp.345-350
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• 2003

A series of aqueous extracts and residues from leaves, stems, roots, pods and seeds of Korean black soybean (Glycine max (L.) Merr.) were assayed against alfalfa (Medicago sativa) and barnyard grass (Echinochloa crus-galli) to determine their allelopathic activities through petri-dish and greenhouse experiments, and the results showed highest inhibition in the extracts or residues from the seeds, and followed by pods. The extracts of 40g dry tissue $\textrm{L}^{-1}$ applied on filter paper in petri-dish bioassay significantly inhibited root growth of alfalfa, and especially extracts from seeds and pods reduced root length of alfalfa more than those from leaves, stems, or roots. Plant height, root length, shoot and root dry weights of barnyard grass were reduced significantly by residue incorporation of seeds and pods as the incorporated amount increased. These results suggest that black soybean plants had herbicidal potential, and their activities were exhibited differently depending on plant parts.

• Plant Biotechnology Reports
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• v.3 no.4
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• pp.301-307
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• 2009

To understand the adaptability of alfalfa (Medicago sativa L.) to chilling stress, we analyzed the antioxidative mechanism during seed germination. The germination rates of six alfalfa cultivars were studied comparatively at $10^{\circ}C$. Xinmu No. 1 and Northstar were selected as chilling stress-tolerant and stress-sensitive cultivars for further characterization. After chilling treatment, Xinmu No. 1 showed higher seedling growth than Northstar. Xinmu No. 1 exhibited low levels of hydrogen peroxide and lipid peroxidation compared with Northstar. In addition, shoots in Xinmu No. 1 treated with chilling showed higher activities of the superoxide dismutase, ascorbate peroxidase (APX), and catalase than those of Northstar, whereas Xinmu No. 1 showed higher APX activity in roots that Northstar. These results indicated that high antioxidation activity in Xinmu No. 1 under chilling stress is well associated with tolerance to chilling condition during germination.

• Journal of Korea Soil Environment Society
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• v.4 no.2
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• pp.127-136
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• 1999

In the past several years phytoremediation, defined as the use of plants for removing contaminants from media such as soils or water, has attracted a great deal of interest as a potentially useful remediation technology We attempted to assess the effectiveness of phytoremediation of diesel-contaminated soils in a green house. Screening test for selecting an appropriate plant was performed by observing the harmful effects of diesel dosage on the growth of 4 plants. Alfalfa was selected as a potentially useful plant among corn and barnyard grasses due to its high tolerance to the toxicity of diesel in growth. Bioremediation of the artificial diesel-contaminated soil packed in the PVC columns(0.3m in diameter $\times$ 1m in length) with air supplied, alfalfa planted, and alfalfa and air supplied was investigated for 100 days. The results of the column test showed plant effects on enhancing the biodegradation of diesel in the contaminated soils compared to the control column which had no plant. Injecting air to the columns during phytoremediation also showed additional effects on the removal rate of diesel. Comparison of microbial activity in each test column showed a beneficial effect of plants in the soil remediation processes. This results can be explained microbial activity in rhizosphere is a crucial factor for removing diesel.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.4
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• pp.291-302
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• 1998

The purpose of this study is to clarify the effect of plant growth regulator "Uniconazole-P" on the control of growth and seed producrtion of pasture plants under grown in sward conditions. Four species examined were orchard grass, timothy, red clover and alfalfa. Uniconazole-P concentrations were control(0), 20ppm and 40ppm, and foliar sprayed on canopy structures at the floral differentiation stages of grasses and at the begining of flowering stages of legumes, respectively. 1. Yield components and seed yield components of grasses and legumes were responded differently between Uniconazole-P concentrations, species and the stages of growth. 2. At early heading stages, the plant length and culm length of grasses were reduced by Uniconazole-P treatments. On the contrary, the dry weight of ears per area and chlorophyll concentrations were increased by Uniconazole-P treatments. 3. At seed ripening stages, the number of ears, dry weight of a tiller, dry weight of a ear, dry weight of ears per area and dry weight of seeds in orchardgrass, and the number of ears, dry weight of a ear, dry weight of ears per area, dry weight of seeds and harvest index in timothy were increased by Uniconazole-P treatments. 4. At early flowering stages, the plant length and total length of internodes were reduced by Uniconazole-P treatments. On the contrary, total length of branches and chlorophyll concentrations of red clover and alfalfa were increased by Uniconazole-P treatments. Particularly, the number of inflorescences and dry weight of inflorescences of red clover was increased greatly by Uniconazole-P treatments. 5. At seed ripening stages, the plant length of both of legumes were reduced by Uniconazole-P treatments. On the contrary, the dry weight of a inflorescence, dry weight of inflorescences per area, dry weight of seeds and harvest index of alfalfa was increased by Uniconazole-P treatments. 6. Seed production of grasses by Uniconazole-P treatments can be explained as following processes at each stage of growth. 1) reduced in plant length and culm lengths at early heading stages, 2) increased in number of ears and dry weight of a ear at both of stages, and 3) increased in dry weight of ears per area, dry weight of seeds and harvest index at seed ripening stages. 7. Seed production of legumes by Uniconazole-P treatments can be explained as following processes at each stage of growth. 1) reduced in plant length and total length of internodes and increased in number of branches and total length of branches at early flowering stages, 2) increased in number of inflorescences and dry weight of inflorescences at both of stages, and 3) increased in dry weight of seeds and harvest index at seed ripening stages.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.2
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• pp.218-224
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• 2009

In organic fanning systems, integration of cover crops into cropping system are recommended to improve the soil quality, to prevent soil erosion, and to provide biological control of weeds. The aim of the present study was to evaluate the optimal seeding dates of major cover crops. To know optimal seeding dates, crimson clover, alfalfa, rye and hairy vetch were sown on 20. Sep., 27. Sep., 4. Oct., 11. Oct., 18. Oct., 25. Oct. Rye could germinate and grow well in all the tested sowing dates. Hence, it was concluded that the sowing of rye could be conducted later in fall or more earlier in spring than any other cover crops tested in this study. Growth and ground covering capacity were significantly decreased by late sowing in alfalfa and crimson clover. For safe overwintering, alfalfa and crimson clover should be sown before late September. Similarly, field emergence of hairy vetch was found to be sensitive to soil temperature, however, its growth and ground covering capacity after overwintering was enough to compensate the delay in sowing. Germination tests in laboratory revealed that hairy vetch grew faster at $25^{\circ}C$ compared to $20^{\circ}C$. The result suggested that germination and growth rate of hairy vetch was rapidly decreased at low temperature. Therefore, it is recommended that hairy vetch should be sown as soon as possible in September for improving stand establishment after overwintering.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.3
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• pp.369-378
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• 2018

Objective: The impact of forage feeding strategy on growth performance, ruminal fermentation and nutrient digestibility in post-weaning calves was investigated. Methods: Forty-five female Holstein calves (body weight [BW] = $79.79{\pm}0.38kg$) were enrolled in the 35-d study at one week after weaning and randomly assigned to one of three dietary treatments. All diets were fed as total mixed ration containing 60% (dry matter [DM] basis) of basal starter feed and 40% (DM basis) of forage, but varied in composition of forage source including i) alfalfa (40% DM, AH); ii) alfalfa hay (26.7% DM)+oat hay (13.3% DM; OH); iii) alfalfa hay (26.7% DM)+corn silage (13.3% DM; WS). Results: Dry matter intake was not different among treatment groups (p>0.05). However, BW (p<0.05) and average daily gain (p<0.05) of calves fed AH and OH were greater than WS-fed calves, whereas heart girth was greater in OH-fed calves than those fed AH and WS (p<0.05). Ruminal fermentation parameters including proportion of butyric acid, acetated-to-propionate ratio, concentration of total volatile fatty acid, protozoal protein, bacterial protein, and microbial protein in rumen were the highest in OH (p<0.05) and the lowest in WS. Compared with the AH and WS, feeding oat hay to postweaning calves increased crude protein digestibility (p<0.05), and decreased duration of diarrhea (p<0.05) and fecal index (p<0.05). Conclusion: Our results suggested that partially replacing alfalfa hay with oat hay improved ruminal fermentation, nitrogen utilization, and reduced incidence of diarrhea in post-weaning dairy calves.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.1
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• pp.50-55
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• 2003

Herbicidal activity of $\delta$-aminolevulinic acid(ALA), an intermediate for the biosynthesis of tetrapyrroles such as chlorophyll, heme, bacteriochlorophyll, and vitamin $\textrm{B}_{12}$ analogues, was examined to determine the variation in phytotoxic potential against different plant species as affected by different application methods. Seed-soaking treatment, ALA at low concentrations did not affect shoot and root lengths of test plants while at highest concentration reduced them by 20 to 30%. Alfalfa showed the most tolerant response to ALA in both pre- and post-emergence application, and followed by rice. When applied with pre-emergence, cotyledons of Chinese cabbage were severely bleached with 0.5 mM of ALA at 24 hrs after application, and root growth of rice, barnyard grass, and alfalfa was significantly inhibited with increasing of concentration. With post-emergence application, ALA at 2 to 4 mM reduced shoot and root growths of Chinese cabbage and barnyard grass completely. Herbicidal effects of ALA were more enhanced in the treatment combined with 2,2-dipyridyl sthan single application in barnyard grass and Chinese cabbage. The results suggest that alfalfa was the most tolerant to ALA among the tested plants, and that post-emergence application of ALA exhibited greatest photodynamic activity against tested plants.

• Korean Journal of Organic Agriculture
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• v.20 no.4
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• pp.589-605
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• 2012

This study was conducted to identify allelopathic effect of Ganghwa domestic Artemisia spp., named Sajabalssuk and Ssajuarissuk, for various receptor plants including clover (Trifolium repens L.), alfalfa (Medicago sativa L.), lawn grass (Zoysia japonica Steud.), dandelion (Taraxacum platycarpum Dahlst.), and dahurianpatrinia (Patrinia scabiosaefolia Fisch. ex Trevir). Receptor plants were treated with the aqueous and essential oil extract of Artemisia plants. In consequence, their allelopathic effects were evaluated by measuring seed germination rates, seedling growth, and dry weights of the receptor plants. The seed germination and seedling growth of the receptor plants were inhibited by all treatments of both aqueous and essential oil extracts of the Artemisia plants, and, in addition, the inhibitory effects were increased according to the higher concentration. Among the donor plants, A. $sp.^*III$ showed most effective allelopathic effect. Comparing the alleopathic effect among the receptor plants, seed germination was most inhibited in lawn grass while inhibitory effect of seedling growth was comparatively higher in dandelion. Although inhibitory effects were comparatively lower, the allelopathic effects of Artemisia plants were identified in clover and alfalfa since the seedling growth of these plants were inhibited more than 70%. Thus, in result, Ganghwa domestic Artemisia spp. could be possibly used for weed control since natural products of the plants showed inhibitory effects on seed germination and seedling growth of various receptor plants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.2
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• pp.113-120
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• 1996

The experiment was carried out to investigate the effects of phosphate fertilizer level on growth, crude protein content and nitrogen fixation ability of alfalfa(Medicugo sariva L.) at sowing year. The experimental field was conducted using a randomized block design with three replications according to the phosphate application level (200 and 400kfia) in farm of Kongju National University. Research samples were collected at intervals of 7 days from October 7 to December 9 and nitrogen fixation activity of nodule was measured by using the acetylene reduction method (Hardy et al. 1968). The results obtained are summarized as follows : 1. Number of branches, root length and dry matter weight at phosphate fertilizer level of 400kg in sowing year were more increase than those of 200kfia. The number of nodules was not significantly difference between phosphate fertilizer level, because of low temperature to be enough to nodulation of alfalfa. 2. The crude protein content of leaf, stem and root were not significantly affected by the phosphate fertilizer level. but that of leaf was increased at 400kgha on 72 days after sowing. The crude protein content of each part according to growing stages was increased at 44 days and 72 days after sowing. 3. The dry weight of root was not significantly difference between phosphate fertilizer level from 30 days to 51 days after sowing, but that was increased at phosphate fertilizer level of 400kgha from 51 days afier sowing. 4. The amount of ethylene in the samples taken 2 hours and calculated amount of nitrogen fixation were increased at phosphate fertilizer level of 400kfia from 30 days to 65 days after sowing. but there was vice versa from 58 days after sowing.

• Environmental Analysis Health and Toxicology
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• v.17 no.1
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• pp.29-35
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• 2002

The test of germination has been used as a good indicator to assess the toxicity of chemicals to plant. This study was carried out to investigate the effect of PAHs, anthracene and phenanthrene, on germination ratio, germination index and germination time of barley, tall fescue and alfafa. Anthracene and phenathrene induced to decrease the seedling length and increase the germination time of the experimental plants. But they did not affect the maximum germination ratio. While it was not correlated between seedling length and final germination ratio, it was shown a close relationship between seedling length and mean germination time of the plants. These results suggested that the mean of germination time and seedling growth could be useful to exmine the phyto -toxicological effect of PAHs. The mixture of anthracene and phenanthrene was examined to investigate the combined effect on seedling length; the mixture induced the reduction of seedling. This was meant that the mixture toxicity of PAHs might be not shown in the sum of each chemical toxicity.