• Title/Summary/Keyword: growing stages

Search Result 518, Processing Time 0.026 seconds

Studies on Eriophyes kuko Kishida and its Galls III. Quantitative Changes of Nucleic Acid in Growing Galls (구기자혹응애(Eriophyes kuko Kishida) 및 그 혹에 관한 연구 III. 혹의 성장에 따르는 핵산함량의 변동)

  • Kim Chang Hyo
    • Korean journal of applied entomology
    • /
    • v.10 no.2
    • /
    • pp.103-107
    • /
    • 1971
  • In this report, an experiment has been conducted to test the quantitative changes of nucleic acids in the nuclei of the epidermal cell of the galls, caused by Eriophyes kuko Kishida on the leaf of Lycium chinense Mill by means o( microspectrophotometric techniques, the two-wave-length methods. And the sizes of the epidermal cells and nuclei have been measured. The experimental results were summarized as follows: 1) It has been found that as the gall grows, the diameter of the epidermal cells and their nuclei increased and they were larger than those of tile healthy ones. 2) Microspectrophotometric measurement of nuclei by the 'Two-wave-length method' after staining with Feulgen reagent showed no changes in DNA content in the early stage of the gall. As the gall matured, however, DNA content of the gall increased more than that of the healthy leaf. 3) RNA-measurement of nuclei stained with Azur-B in DNase treated epidermal cells of the gall revealed that temporary increase in RNA content occurred in early to middle stages after the gall formation. As the gall matured, however, RNA content of the gall decreased more as against that of the healthy leaf.

  • PDF

THE CHANGES OF TELOMERASE ACTIVITY AND PROLIFERATING CELL NUCLEAR ANTIGEN(PCNA) EXPRESSION IN THE DEVELOPMENTAL STAGES OF RAT'S UPPER DIGESTIVE SYSTEM (백서 상부 소화기계의 발달 시기별 Telomerase 활성 및 PCNA(proliferating cell nuclear antigen)의 발현 변화)

  • Sung, Iel-Yong;Kim, Jong-Ryoul
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
    • /
    • v.30 no.2
    • /
    • pp.85-99
    • /
    • 2004
  • Purpose: This study observed the changes in the telomerase activity, it's developmental regulation, PCNA expression, and their correlation in rat's upper digestive organs during growth and aging. Materials and Methods: Upper digestive organs(buccal mucosa, gingiva, palate, submandibular and parotid glands, and tongue) were aseptically removed from Sprague-Dawley rats of fetal(gestational 20 days), growing(1, 2, 3, 5, and 7 weeks after birth) and adult(12 week old). Samples for telomerase activity were frozen on liquid nitrogen immediately after sacrifice, and stored until the use at $-75^{\circ}C$ in order to measure it. Telomerase activity was measured by a PCR-based telomeric repeat amplication protoco(TRAP) assay and quantitated with Photometric Telo TAGGG Telomerase PCR ELISA plus(Roche Diagnostics GmbH. Mannheim. Germany). PCNA expression were measured immunohistochemistry with anti PCNA Ab-1, Clone PC10(NeoMark. California. USA). Results: 1. Telomerase activities in buccal mucosa, palate and gingiva were the highest in fetus and decreased gradually or rapidly after birth and then diminished, but In salivary gland and tongue were the highest in fetus and also high at 1 week and then decreased rapidly. 2. PCNA expression in buccal mucosa, gingiva, Tongue and salivary gland was the highest in fetus and decreased gradually and then diminished. but only in palate decreased rapidly after birth and then diminished. Conclusion: The highest telomerase activity of embryonic stage decreased rapidly after birth in rat's upper digestive organs. There may be a developmental regulation of telomerase activity, but not a tissue-specific. This telomerase activity seems correlated closely with PCNA expression in rat's upper digestive system.

Study on post-flutter state of streamlined steel box girder based on 2 DOF coupling flutter theory

  • Guo, Junfeng;Zheng, Shixiong;Zhu, Jinbo;Tang, Yu;Hong, Chengjing
    • Wind and Structures
    • /
    • v.25 no.4
    • /
    • pp.343-360
    • /
    • 2017
  • The post-flutter state of streamlined steel box girder is studied in this paper. Firstly, the nonlinear aerodynamic self-excited forces of the bridge deck cross section were investigated by CFD dynamic mesh technique and then the nonlinear flutter derivatives were identified on this basis. Secondly, based on the 2-degree-of-freedom (DOF) coupling flutter theory, the torsional amplitude and the nonlinear flutter derivatives were introduced into the traditional direct flutter calculation method, and the original program was improved to the "post-flutter state analysis program" so that it can predict not only the critical flutter velocity but also the movement of the girder in the post-flutter state. Finally, wind tunnel tests were set to verify the method proposed in this paper. The results show that the effect of vertical amplitude on the nonlinear flutter derivatives is negligible, but the torsional amplitude is not; with the increase of wind speed, the post-flutter state of streamlined steel box girder includes four stages, namely, "little amplitude zone", "step amplitude zone", "linearly growing amplitude zone" and "divergence zone"; damping ratio has limited effect on the critical flutter velocity and the steady state response in the post-flutter state; after flutter occurs, the vibration form is a single frequency vibration coupled with torsional and vertical DOF.

Burden Assessment of Thyroid cancer in Iran from 1990 to 2010: Lessons Obtained from Global Burden of Disease Report 2010

  • Modirian, Mitra;Cheraghi, Zahra;Rahimzadeh, Shadi;Moghaddam, Sahar Saeedi;Jarrahi, Alireza Mosavi
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.16 no.17
    • /
    • pp.7743-7748
    • /
    • 2015
  • Background: Thyroid tumors are generally regarded as rare malignancies. Nowadays, however, their global incidence is growing continuously partially due to western life style and utilization of more sensitive methods of early detection. It is approximately three times more prevalent in females than in males. Most cases of thyroid cancer are asymptomatic nodules or just have local cervical symptoms or adenopathy in early stages. Materials and Methods: The Global Burden of Diseases report 2010 study (released 3/2013) profited from 100 collaborators worldwide and used a vast network of data on health outcomes, vital registries, and population surveys. It shared many of the Global Burden of Diseases 1990 principal databases such as all available data on injuries, diseases, risk factors, as well as comparable metrics, and used different scientific approved methods to estimate important health status data like: death rate, life expectancy, healthy adjusted life expectancy, disability-adjusted life years (DALY), years of living lost due to premature death and years of life with disabilities. Results: DALY as thyroid cancer burden per 100,000 Iranian populations had increased by about 14% during 1990 to 2010 in all ages; from 6.1 (95% UI 4.2-9.74) years in 1990 to 6.95 (95% UI 5.06-9.18) years in 2010 in both sex. The 2010 peak age-group was estimated at 45-49 years in males and 40-45 years in females.

Poultry industry of Bangladesh: entering a new phase

  • Rahman, Md Shahidur;Jang, Dong-Heon;Yu, Chan-Ju
    • Korean Journal of Agricultural Science
    • /
    • v.44 no.2
    • /
    • pp.272-282
    • /
    • 2017
  • In Bangladesh, since the beginning of 21st century, the poultry industry has become an unparalleled platform for a quick profit, the generation of local employment, and the production of cheaper animal proteins. This paper summarizes poultry industry-related facts and figures for Bangladesh, starting from the very beginnings of this sector when poultry farming was part of subsistence family farming only to today's highly commercialized horizon, through personal experiences, secondary data analysis, spot visits, and the sharing of views and ideas with stakeholders. A critical analysis reveals that the poultry industry in Bangladesh may become multi-dimensional and more stable in the near future as it establishes sufficient backward and forward linkages to input and output supplies. We can foresee its passing the very fast growing juvenile stages of higher net profit earning and less responsible then becoming fully mature. The projected demand and production potentials of this sector are increasing, but at a slower pace than before, in the face of some important challenges like emerging disease, higher prices of inputs, consumer preferences, and strong market competition. However, evidence suggests that the production systems of poultry in Bangladesh are entering a new era to cater to the needs of consumers, society, and government as it produces healthier and more diversified, further-processed foods, in the coming years. A better economy in the country, a more health-conscious population, and the availability of new and affordable innovations in this sector might be the driving forces to support the foreseen transformations.

Consideration of Urgent Facing Strategies for the Designation of Customs Free Zone of Incheon City (인천항 관세자유지역 지정시 당면과제 고찰)

  • 박창호;이기철;노흥승;여기태
    • Journal of Korea Port Economic Association
    • /
    • v.17 no.2
    • /
    • pp.183-204
    • /
    • 2001
  • This study is intended for the development of urgent facing strategies for the designation of a CFZ(Customs Free Zone) in Incheon city. First, it analyzes the current status of the Incheon CFZ. Next, it suggests several strategies for successfully growing the CFZ of Incheon City. Finally, it develops and proposes a method for establishing an integrated management system for the effective management of the CFZ. The key findings of this study are as follows: First, the CFZ should provide state-of-the-art movement of port logistics, create new added value caused by the movement of logistics, and provide other public support. The Incheon CFZ should be developed in three stages: an inducement stage in 2002-2005, a growth stage in 2005-2008, and an implementation stage in 2008-2011. Second, The parties responsible for the management of the CFZ would be as follows: for the port area, the Ministry of Maritime and Fisheries; for the back space of CFZ, Incheon City; and for port management, port management companies. However, a committee for CFZ management is strongly suggested for the improved efficiency and effectiveness of CFZ management. Furthermore, additional regulations ensuring the role of local government will likely be necessary for the sucessful designation and sustainable management of the CFZ. Third, For the creation of additional revenue, reformation of the current laws of the CFZ are recommended in the area of business administration. Fourth, A strategic marketing plan that supports the successful management of the companies of the CFZ will be a key component in the success of the CFZ. Thus, the new marketing plan should be developed in the near future based on case studies of other CFZs. Fifth A method for an integrated CFZ management system was developed and suggested for the comprehensive and transparent management of port logistics.

  • PDF

Exploring Responses to Light in the Monocot Model Plant, Brachypodium distachyon

  • Tran, Quynh-Giao;Han, Yun-Jeong;Hwang, Ok-Jin;Hoang, Quyen T.N.;Kim, Jeong-Il
    • Korean Journal of Plant Resources
    • /
    • v.31 no.5
    • /
    • pp.522-530
    • /
    • 2018
  • Brachypodium distachyon has been developed as a monocot model plant for temperate grasses and bioenergy crops. Although B. distachyon research is moving forward rapidly, the study of photoresponses has not been explored. To extend our knowledge of responses to light in monocots, we performed photoresponse analysis of B. distachyon using two inbred lines, Bd21 and Bd21-3. In this study, we first compared growing phenotypes between the two lines and investigated coleoptile and primary leaf growths under dark, far-red, red, and white light conditions. The results showed that the growth of the two lines were similar until tillering stage, but other developmental stages from heading to senescence were much delayed in Bd21-3, which resulted in increased height and tiller numbers. Under different light conditions, primary leaf lengths were kept increasing during the growth period, whereas the coleoptile extension was inhibited 4 to 7 days after growth depending on the light conditions applied. These results suggest that the responses to light in B. distachyon can be examined by measuring coleoptile lengths approximately 7 days after seedling growth. Moreover, we selected light-responsive genes known in Arabidopsis thaliana, such as chlorophyll A/B binding protein (CAB), light-harvesting chlorophyll binding protein (Lhcb) and chalcone synthase (CHS), and confirmed their light-induced gene expression in B. distachyon. Therefore, the present study suggests that the inhibition of coleoptile growth can be used as the parameter to analyze photoresponses in the monocot model plant, and also provide the reference genes whose expression is induced by far-red and red light treatment.

Yield and Nitrogen Uptake under Reduced Nitrogen Fertilizer during Early Growth of Rice in the Rice-Barley Double Cropping System

  • Seo, Jong-Ho;Cho, Hyeon-Suk;Kim, Chung-Guk;Lee, Jin-Mo;Park, Seong-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.49 no.1
    • /
    • pp.1-6
    • /
    • 2004
  • N fertilizer required by rice could be reduced greatly in the rice-barley double cropping system than in the rice single cropping system. This study was conducted to investigate how much of the N fertilizer during the early stage of rice in the rice-barley double cropping system, could be saved compared to that in the rice single cropping system. This experiment was carried out at the paddy field of the National Crop Experiment Station in Suwon, Korea during three years from 1999 to 2001. Amounts of soil mineral nitrogen (SMN) and SPAD values of rice leaf during rice growing season in the rice-barley double cropping system were higher than those in the rice single cropping system under the same amount of N application during two years. Yield and N uptakes of rice at harvesting time were also higher in the rice-barley double cropping system than in the rice single cropping system during two years. Yield and N uptake of rice in the rice single cropping system were decreased when basal N fertilizer was omitted, but those reductions were not found by either omitting basal N fertilizer or omitting N fertilizer at tillering stage in the rice-barley double cropping system during 2000 and 2001. But yield and N uptakes of rice were decreased by 70 kg/10a and 2kgN/10a by the omission of both N application at basal and tillering stages in the rice-barley double cropping system in 2002. It was concluded that N fertilizer as much as tillering N fertilizer could be saved in the rice-barley double cropping system.

Effects of Renewal Pattern of Recycled Nutrient Solution on the Ion Balance in Nutrient Solutions and Root Media and the Growth and Ion Uptake of Paprika (Capsicum annuum L.) in Closed Soilless Cultures

  • Ko, Myat Thaint;Ahn, Tae In;Shin, Jong Hwa;Son, Jung Eek
    • Horticultural Science & Technology
    • /
    • v.32 no.4
    • /
    • pp.463-472
    • /
    • 2014
  • Ion imbalance in recycled nutrient solutions is caused by selective ion uptake of plants, which occurs at different rates in different growth stages. The objectives of this study were to investigate the ion balances in both recycled nutrient solutions and rockwool media using different renewal patterns for the nutrient solutions, and to analyze the subsequent effects on uptake of water and nutrients. Over 12 weeks of paprika cultivation, two different renewal patterns (week units) of 6-4-2 and 8-2-2 weeks were compared with a constant renewal pattern of 4-4-4 weeks (control). The nutrient solution in the reservoir tank was constantly maintained at EC $2.5dS{\cdot}m^{-1}$ and pH 5.5-6.5. The changes in the ion balance with the 4-4-4 week pattern were smaller than those with the other treatments. In the early growth stage, however, the ion balances similarly changed among all treatments. Greater changes were subsequently observed for the 6-4-2 week pattern. Although fruit yield and shoot fresh weight of paprika were the lowest with 6-4-2 renewal pattern, no significant differences were observed. Our results indicate that renewal intervals can be extended in consideration of growth stage for more efficient and practical operations in closed soilless cultures.

Sexual Maturity and Reproductive Cycle of Starry Flounder Platichthys stellatus Cultured in Indoor Tank (실내 사육한 강도다리 Platichthys stellatus의 성성숙과 생식주기)

  • Lim, Han-Kyu;Byun, Soon-Gyu;Lee, Jong-Ha;Park, Sang-Un;Kim, Yi-Cheong;Han, Hyoung-Kyun;Min, Byung-Hwa;Lee, Bea-Yik
    • Journal of Aquaculture
    • /
    • v.20 no.4
    • /
    • pp.212-218
    • /
    • 2007
  • Reproductive biology (sexual maturity and reproductive cycle) of starry flounder Platichthys stellatus were examined by histological methods. From March 2004 to February 2005, 144 females and males were sampled. Gonadosomatic index (GSI) of female and male were peaked in May and February, respectively. Monthly changes of hepatosomotic index (HSI) showed a negative correlationwith those of the GSI. Changes of condition factor (CF) in female were correlated with gonad maturation, while that of male were no difference all the year round. Based on monthly GSI and gonadal development the reproductive cycle of starry flounder could be divided into four stages: growing stage (September to November), maturation stage (September to February), ripe and spent stage (March to May), and recovery and resting stage (June to August). Biological minimum size of female was 1,074 g. The relationship between fecundity (F) and body weight (BW) of the fish was expressed as $F\;=\;455.86BW^{1.2006}$.