• Korean Journal of Soil Science and Fertilizer
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• v.44 no.5
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• pp.819-823
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• 2011

The present study evaluated the seasonal changes of the soil microbial population by selected media in an organic farming system (OFS) with rye rotation cropping management compared to those in a conventional farming system (CFS) with chemical fertilizers, pesticide and herbicide from May 2009 to October 2010 in an upland field. With the exception of fungi, populations of aerobic bacteria, Gram-negative bacteria, and Bacillus spp. were higher in the OFS soil during soybean-growing stages. In addition, populations of aerobic bacteria, Gram-negative bacteria, and Bacillus spp. in the OFS soil were nearly two times more than those in the CFS soil on reproductive growth stages. Our findings suggested that Bacillus spp. should be considered as responsible factor for microbial population differentiation observed between the OFS and the CFS in upland fields.

• Journal of Korea Society of Digital Industry and Information Management
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• v.18 no.2
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• pp.93-110
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• 2022

The purpose of this study is to explore business investment and operation of O2O (Online-To-Offline) combined service. The study will analyze the necessary factors for growing the business by dividing the O2O service by industry. The Online-to-Offline is a method of inducing purchases of products and services by connecting between online and offline This research methodology organized the four stages of the analysis process. The analysis of all stages was performed with association rules in big data techniques. It is divided into the start-up period, growth period, maturity period, and decline period, and analysis is conducted on the business investment, expenditure cost, business operation, and conflict factors. As the research result, the first analysis has shown commonality with government subsidies, bank loans, and personal funds in all industries. The second analysis showed a lot of expenditure on labor costs of internal employees, marketing/sales, facility facilities, equipment, and equipment purchase costs. The third analysis showed difficulty in raising the investment resources necessary for business operations in all industries. The last analysis showed conflicts in the industry, businesses license, legal systems, and small business owners in all industries. This study contributed to the abundance and diversity of research methodologies in management information systems using association rules. In addition, the description of organizational development theory was updated while explaining the business investment and operation of O2O combined services. In practical implication, the O2O services include environmental factors that cause convergence between industries. Accordingly, this is required for new O2O services through new laws and systems and reorganization of existing laws and regulations.

• Journal of Plant Biotechnology
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• v.6 no.1
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• pp.15-19
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• 2004

The expression of a chimeric transgene (nos-npt II) has been examined in 9 years old transgenic poplars (Populus koreana x P. nigra) growing in a nursery. The expression of the gene in twenty six independentely transformed plants were examined by 1) enzyme (NPT II) assay, 2) RT-PCR, and 3) resistance to kanamycin. High NPT II activities in young leaves of all the transformed plants were found even without a selection pressure for antibiotics for 9 years. However, the activity varied with the positions of leaves in the stem in that young leaves showed higher activity than did mature tissues. When leaf segments were cultured in the presence of 150 mg/l kanamycin, only those from young leaves produced vigorously growing callus. However, as in the case of NPTII assay, the leaf segments from mature leaves did not form callus well on the media. RT-PCR with nptII specific primers also showed that amplification products were observed only when RNAs from young tissues were used. The total RNA gel showed that while RNA in young leaves are relatively stable and in a large quantity, those in old leaves were mostly degraded. All the above results suggest that the gene is transcriptionally active only in young tissue even though it is attached to a constituitive promoter. Therefore, the expression of foreign gene in poplar plants seemed to be affected by the metabolic state of the cells and thus vary greatly with the developmental stages and the age of tissue.

• Korean Journal of Agricultural Science
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• v.12 no.2
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• pp.174-182
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• 1985

In order to obtain information necessary for attaining maximum yield of rice by increasing nitrogen efficiency, effects of top dressing of nitrogen fertilizer at the later part of growing season were investigated and results obtained are summurized as follows: 1. The increased application of nitrogen fertilizer was very effective in increasing leaf areas and net assimilation rate of rice plants. The spikelets number per hill was also increased due to heavy nitrogen fertilizer. 2. The concentrated top dressing at the later part of plant growth apparently increased the crop growth rate at the reproduction stage and ripening stage of rice, respectively. The nitrogen efficiency was greatly improved by the concentrated top nitrogen dressing through the increase of effective tiller number and the improvement of production organ to non productive organ ratio. 3. The concentrated top nitrogen dressing at the later growing season was considered to be one of the most effective methods of fertilizer applications as well as cultivation of fertilizer resistant varieties for the high yield of rice and improving soil fertility.

• The Korean Journal of Ecology
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• v.19 no.4
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• pp.341-351
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• 1996

In order to elucidate the differences in early successional development among similary aged old-fields having different soil nitrogen (N), caused by the land use history before at abandonment, the response of plant community along an experimental nitrogen gradient (control plot (No), plot NI with 5.8g $N/m^2$, plot N2 with 11.7g $N/m^2$ and plot N3 with 23.3g $N/m^2$) was investigated in a five-year-old abandoned field. Although the N content in soil among treatments was similar at the end of the growing season, N concentrations in plant tissue increased with the amount of N supplied. These results suggest that almost all the N contained in N-enriched soil might be absorbed by plants during the growing season after N supply. Vegetation tended to grow vigorously by nitrogen supply, and the standing biomass increased significantly in plots NI and N2 . Species richness of plants, especially of annuals and perennials, was more reduced than the control plot, and the species diversity was also reduced by N supply. The importance value (IV) of species by N supply differed in each species along the position on the successional sere: Artemisia princeps var. orientalis as the dominant species in this old-field decreased slightly; annuals as the earlier successional species decreased clearly along nitrogen gardients; Erigeron annuals as the earlier successional species and as a strong competitor with Artemisia princeps var. orientalis had the highest IV by small N supply; Miscanthus sinensis and Rubus crataegifolius as the later successional species increased by large N supply. These results suggest that old-fields with high soil N might show the structural and functional characteristics of the earlier successional stages, but community composition in those old-fields might be changed more quickly from the sarlier successional species than the later successional species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.5
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• pp.393-399
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• 2004

Annual reproductive cycle of Siganus canaliculatus was studied based on monthly variation of gonadosomatic index (GSI) and histological changes of gonads. Samples were monthly collected by a set net along the southern coast of Jeju Island, Korea from January to December, 1996. Variation of the monthly mean GSI values showed similar trends in female and male. The GSI values increased from June and reached a peak in the spawning season in July $(9.65{\pm}1.96\;in\;females,\;10.00{\pm}4.27\;in\;males)$, and decreased rapidly thereafter. Female hepatosomatic index (HSI) values ranged from $1.26{\pm}0.22\;(in\;April)\;to\;2.34{\pm}0.39$ (in July), and male HSI values ranged from $1.27{\pm}0.21\;(in\;April)\;to\;1.87{\pm}0.30$ (in October). Annual reproductive cycle was classified into the following successive stages: in female, growing stage (from February to June), mature stage (from June to July), ripe and spawning stage (from July to August), recovery stage (from August to March); and in male, growing stage (from January to June), mature stage (from June to July), ripe and spent stage (from July to August), and recovery stage (from August to April). Based on these data, this species has a group-synchronous oocyte development and one spawning season a year.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.2
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• pp.221-227
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• 2011

Applications of green manures generally improve the soil quality in rice paddy in part through restructuring of soil microbial communities. To determine how different green manures affect soil microbial communities during the early stages of rice growth, fatty acid methyl ester (FAME) profiles were used to the effects of different management practices: 1) conventional farming (CF), 2) no-treatment (NT), 3) Chinese milk vetch (CMV), 4) green barley (GB), and 5) triticale in paddy field. With applications of green manures, soil organic matter was significantly higher than CF, while soil Na concentration was significantly lower compared with CF (p<0.05). Total soil microbial biomass of CMV was higher (p<0.05) than NF by approximately 31%. The highest ratio of monounsaturated fatty acid to saturated fatty acid was found in the GB plot, followed by CMV and triticale compared with CF (p<0.05), possibly indicating that microbial stress was less in GB and CMV plots. Populations of Gram-negative bacteria and arbuscular mycorrhizal fungi also were significantly higher green manures than CF (p<0.05). Our findings suggest that GB should be considered as optimum green manure for enhancing soil microbial community at an early growing stage in paddy field.

• Korean Journal of Ichthyology
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• v.12 no.1
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• pp.62-70
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• 2000

Annual reproductive cycle of the file fish, Stephanolepis cirrhifer (Temminck et Schlegel), was histologically investigated. Samples were collected monthly in the coastal waters of Chungmun, south of Cheju Island, Korea from July 1997 to June 1999. In males and females of S. cirrhifer, the values of GSI (gonadosomatic index) were similar to the changes of water temperature and day length. GSI values in males and females were reached the maximum in June. Reproductive cycle could be divided into the following successive stages: in females, growing stage (February to May), mature stage (May to June), spawning stage (June to August), degenerative and resting stage (September to December), and in males, growing stage (February to April), mature stage (May to June), spent stage (June to August), degenerative and resting stage (September to January), respectively. Throughout histological observation of the ovary in the spawning period, the species belong to an asynchronous and multiple spawner.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.1
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• pp.27-35
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• 2020

Filefish Thamnaconus modestus has seen a sharp decline in their catch, we must strive for continuous resource management. We investigated the maturation and spawning of T. modestus by trawl net and set net from January 2018 to November 2019, off Jeju Is. and in coastal waters of North Gyeongsang Province's Korea. We analyzed monthly change total length (cm), gonadosomatic index (GSI) and maturity stages, egg diameter (mm), the relations fecundity and total length. GSI for Jeju Is. was the highest in April (3.17, 2.43), and decreased from July to 1.73 in August and GyeongBuk's GSI was the highest in Apri l (1.86, 2.58), followed by the high in May and the sharp decline in September, which is 0.23. Using a histological method, the annual reproductive cycle of T. modestus can be divided into 5stage in females; the early growing stage (Jan.-Mar.), late growing stage (Apr.-May), Mature stage, spent stage (Oct.), Recover and resting stage (Nov.-Jan.).We estimate the TL at 50% maturity as 24.1cm for female, fecundity ranged from 560,044 eggs at 23.4cm total length to 1,580,387 eggs at 36.6.cm TL.

• Development and Reproduction
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• v.23 no.4
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• pp.323-331
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• 2019

In this study, an experiment was conducted to investigate the annual reproductive cycle of a Korean flat bittering, Acheilognathus rhombeus, from Ogok-myeon located in Seomjin River. The reproductive cycle was examined histologically regarding water temperature and day length of the habitat, the gonadosomatic index (GSI), and developmental characteristics of female and male gonads. The maximum GSI was found to be 3.50±0.53 and 1.36±0.14 for females and males, respectively, when the water temperature and day light was 16.9℃ and 11.3 hours, respectively in October 2018. On the other hand, the minimum GSI was found to be 0.16±0.09 and 0.69±0.15 for males and females in December 2018 and February 2019, respectively. The ovipositor of females appeared from August to November 2018. We compared and calculated the stages of germ cell developmental characteristics in the testis and ovaries to determine the reproductive cycle. According to the result, we classified the female A. rhombeus reproductive cycle into four phases, which are ripe and spawning phase (October), degenerative phase (November to December), growing phase (January to March) and mature phase (April to September). The annual reproductive cycle of male A. rhombeus was categorized into four phases: mature phase (June to October), degenerative phase (November to March), resting phase (April) and growing phase (May). The Korean flat bittering is an autumn-spawner as the main spawning season in October. In male, testicular spermatogonia appeared all year-round, and the ripe and releasing phase, which is characteristics of the spawning season in other fish, did not appear.