• Korean Journal of Veterinary Research
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• v.37 no.1
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• pp.71-78
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• 1997

This study was designed to investigate the number of the growing and mature follicles following gonadotrophin treatments for superovulation in mature rats. Eighteen mature rats (Sprague-Duwely, initially 190~230gm) were randomly alloted into 3 groups. One group was control group, another FSH-treated group was injected intramuscularly with 0.5 units of follicular stimulating hormone (FSH) / rat, and third PMS and HCG-treated group was intramuscularly injected with 20~25IU of pregnant mare serum (PMS) / rat and then at the 48 hrs later, with 20~25IU of human chorionic gonadotrophin (HCG) / rat. The uteri and ovaries of rats were collected and then were observed grossly and serial sections of paraffin embedding ovaries were stained with H-E. Number of ovarian follicles by following 3 grades of large, middle and small follicles from secondary and tertiary follicles were investigated by LM photography of preparations. Small follicles were classified as secondary follicles of preantral follicles with more than 2 layers of granulosa cells surrounding the oocyte and middle follicles were classified as secondary follicles with early signs of antral cavity or with more than one small cavity on either side of the oocytes and large follicles were classified as tertiary follicles with a single medium sized antral cavity or large well-formed antral cavity. In gross findings, the uteri were slightly swelling in FSH-treated group and markedly swelling or filled with fluid in the uterine lumen in PMS and HCG-treated group. In histological findings, the shape and size of the follicles were diverse in middle and large follicles of FSH-treated group and PMS and HCG-treated group, and proportion of atretic follicles was increased in FSH-treated group and PMS and HCG-treated group than those in control group. The uteri of FSH-treated group and PMS and HCG-treated group were hypertropied or filled with fluid in the lumens and walls of uteri. The wall tissue layers were flattened and their blood and lymph vessels were dilated. The mean number of follicle per ovary in control group were appeared to be $17.1{\pm}5.6$($14.0%{\pm}4.6%$), $37.8{\pm}9.1$($30.9{\pm}7.4%$) and $67.6{\pm}30.1$($55.2{\pm}24.6%$) respectively at large, middle and small follicles and total number of these 3 grade follicles were appeared to be $122.5{\pm}40.0$. The mean number of follicle per ovary in FSH-treated group were appeared to be $22.8{\pm}7.0$($17.4%{\pm}5.3%$), $43.4{\pm}6.6$($33.2{\pm}5.1%$) and $64.5{\pm}13.0$($49.3{\pm}9.9%$) respectively at large, middle and small follicles and total number of these 3 grade follicles were appeared to be $130.7{\pm}16.6$. The mean number of follicle per ovary in PMS and HCG-treated group were appeared to be $29.7{\pm}11.0$($16.3%{\pm}6.0%$), $61.9{\pm}17.2$($33.9{\pm}9.4%$) and $91.1{\pm}28.2$($49.9{\pm}15.4%$) respectively at large, middle and small follicles and total number of these 3 grade follicles were appeared to be $182.6{\pm}32.7$. The above findings reveal that large follicles were increased 29.8% in FSH-treated group and 73.7% in PMS and HCG-treated group than those in control group and in histologic findings, proportion of atretic follicles were more increased in ovaries with more number of more developing follicles.

• Korean Journal of Microbiology
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• v.49 no.1
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• pp.24-29
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• 2013

In the present study, the effects of prebiotics and prebiotics+probiotics on intestinal microflora and fermentation products were evaluated in a pig in vitro fermentation model. The substrates used in this study were iso-malto oligosaccharide (IMO), partially digested chicory-inulin (CI), raffinose (RA), and cyclodextrin (CD) as prebiotics and Lactobacillus reiteri as probiotics. For a pig in vitro fermentation, the experimental diet for growing pigs was predigested using digestive enzymes secreted by small intestine and this hydrolyzed diet was mixed with a buffer solution containing 5% fresh swine feces. The mixture was then incubated with either prebiotics or prebiotics+probiotics for 24 h. Samples were taken at 24 h, and viable counts of microflora, gas, pH, volatile organic compounds (VOCs) and short-chain fatty acid (SCFA) were analyzed. The viable count of Enterobacteriaceae was significantly decreased (p<0.001) in all treatments containing prebiotics and prebiotics+probiotics when compared to the control. However, the number of lactic acid bacteria increased in the prebiotics and prebiotics+probiotics treatment. The pH values in the fermentation fluid decreased in all treatments when compared to the control, and their effects were greater in the prebiotics+probiotics group than prebiotics group. Fermentation with prebiotics resulted in a reduction in malodorous compounds such as ammonia, hydrogen sulfide and skatole when compared to the prebiotics+probiotics group. Short-chain fatty acid production was also higher for treatment with prebiotics+probiotics than treatment with prebiotics. In conclusion, the results of this study demonstrated that fermentation with prebiotics was effective in reducing the formation of malodorous compounds and prebiotics+probiotics was effective in increasing lactic acid bacteria and SCFA and reducing the pH. Moreover, further studies will be needed to determine whether the results observed in the in vitro model would occur in pigs that ingest these prebiotics or probiotics.

• Journal of Animal Science and Technology
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• v.45 no.4
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• pp.593-606
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• 2003

This study was conducted to develop effective manufacturing methods of a total mixed ration(TMR) composed of broiler litter(BL) and bakery by-product(BB) for ruminants. Five experiments included a small-scaled manufacture of TMR using a deepstacking method(Exp. 1), its pelletization(Exp. 2), its field-scaled manufacture(Exp. 3), a field-scaled manufacture using an ensiling method(Exp. 4), and a mixing process of deepstacked BL and BB prior to feeding(Exp. 5). BL and BB were mixed at a ratio which makes total digestible nutrients of the TMR 69%. For each experiment, temperature, appearance and physico-chemical properties were recorded and analyzed. The chemical composition data revealed that the mixture of BL and BB showed nutritionally additive balance which resulted from a considerable increase(P<0.05) of organic matter and a desirable decrease(P<0.05) of protein and fiber up to the requirement level for growing ‘Hanwoo’ steers. Deepstacking of BL and BB in Exp. 1 and 3 resulted in a sufficient increase of stack temperature for pasteurization, little chemical losses, appearance of white fungi on the surface, and partial charring due to excess stack temperature. For Exp. 2, its pelleting, which was successful using a simple, small-scaled pelletizer, resulted in a little loss(P<0.05) of organic matter and an increase(P<0.05) of indigestible protein(ADF-CP). Ensiling the mixture in Exp. 4 made little effect on chemical composition; however, one month of the ensiling period was not enough for favorable silage parameters. Deepstacking BL alone in Exp. 5 tended(P<0.1) to decrease true protein : NPN ratio and hemicellulose content and increase ADF-CP content due to the heat damage occurred. Deepstacking or ensiling of BL-BB mixtures and simple incorporating of BB into deepstacked BL prior to feeding could be practical and nutrients-preservative methods in TMR manufacture for beef cattle, although ensiling needed further hygienic evaluation.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.4 no.3
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• pp.84-95
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• 2001

This study was conducted to suggest the ecological restoration methods for the decomposed granite roadside cutslopes by Natural Topsoil Restoration Methods(NTRM). Four woody species, 3 shrub species, 4 native grasses, 8 wild flowers and 2 cool-season grasses were used. The experiment was conducted with the purpose of developing ecological restoration techniques by achieving following objectives for restoration by using native woody plants, shrubs, native plants and wild flowers; (1) prevention or reduction of wind and water erosion, (2) provision of food and cover for variety of animal species, (3) improvement of the visual or aesthetic quality of disturbed sites. The main results were summarized as follows. 1. Restoring naturalness to human-altered landscapes are important to protecting the worlds biodiversity. In this study, to restore or create an ecological community use as full a complement of native species as possible. Ecological restoration means returning a specific sites to its predisturbance condition. 2. Some species which are introduced first may inhibit the germination and growth of slower-growing species, or they may prevent the growth of other species whose propagules arrive later. In this study, cool season grasses was used small amount than native plants and woody species, because and Indigofera pseudo-tintoria grows well. Tall fescue and perennial ryegrass shows quick germination, but they could not inhibit the other species growing relatively slow. Pinus thunbergii used at Gonam near the ocean was suppressed by the other plants, so it can be concluded that using container seedlings is effective than seeding. 4. Ailanthus altissima, Albizzia julibrissin, Rhus chinensis Lespedeza cyrtobotrya, and Indigofera pseudo-tinctoria were germinated. In wild flowers, Coreopsis lanceolata, Dianthus sinensis, Coreopsis tinctoria, and Lotus corniculatus var. japonicus grew well and show enough plant height which will not suppressed by shrub and woody plants. After one year, native grasses such as Arundinella hirta, Miscanthus sinensis var. purpurascens and Imperata cylindrica var. koenigii cover the slopes well. 5. 25 herbaceous plants such as Trifolium repens, Arthraxon hispidus, Barbarea orthocerae, Potentilla fragarioides, Lepidium apetalum, Chenopodium album var. centrorubrum, Glycine soja, Rubus crataegifolius and 4 woody species such as Lespedeza maximowiczii, Robinia pseudoacacia, Symplocos Chinensis, Quercus acutissma were invaded. 6. Ecological restoration works by Natural Topsoil Restoration Methods for revegetation of the decomposed granite roadside cutslopes were successfully accomplished. It prevents surface water erosion and shows diverse vegetation structure. It will be successful to restore decomposed granite cutslopes.

• Korean journal of applied entomology
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• v.53 no.4
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• pp.491-495
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• 2014

Mites were collected from organic cultivations of greenhouse cucumbers and identified as Tyrophagus neiswanderi (Acari: caridae). T. neiswanderi (length, $490.1{\mu}m;$ width, $288.1{\mu}m$) is a very small, milky-white, and egg-shaped mite, and it mainly causes damage to the leaves, flowers, and fruits of cucumber plants. In the early growing season of cucumbers, the shoots of seedlings became pale and yellow because of T. neiswanderi, and eventually shrinkage or bud-failing was observed in the plants. In the middle of the growing season, T. neiswanderi caused white spots on the leaves and flowers of the plants, and the spots gradually became holes. T. neiswanderi also caused severe damage to young fruits by feeding on the rinds of the fruits, inducing malformations and lowering the economic value.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.9
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• pp.1315-1319
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• 2003

A study was conducted to investigate the development of the digestive organs (rumen, reticulum, abomasums, small intestine, large intestine and rectum) and other internal organs (liver, spleen, lungs and heart) of Hanwoo (Korean cattle) steers fed diets of compound feed and rice straw by the age from 6 to 30 months old. In the experiment, Two hundreds of Hanwoo steers were allocated in one of two compound feed feeding treatments. The treatment groups were 1) feeding level 1 group fed 1.2 to 1.5% compound feed per kg body weight (BW) in the growing period, 1.7 to 1.8% compound feed per kg BW in the early fattening period, and compound feed ad libitum in the late fattening period and 2) feeding level 2 group fed compound feed ad libitum through the whole period. In every two months, eight steers in each group were slaughtered and the length and weight of the organs of the animals were measured. The weight of the reticulo-rumen was higher (p<0.05) in the group 2 at the age of 14 months. The abomasum weight of group 2 was higher than that of group 1 at the ages of 12 (p<0.01) and 20 months (p<0.05). The weights of the liver, lung and heart of steers in the group 2 were higher (p<0.05) than those in group 1 before 18 months old. At the ages of 8 (p<0.1), 10 (p<0.001), 12 (p<0.01), 16 (p<0.01) and 24 months (p<0.05), the abomasum of steers in the group 1 was longer than that in the group 2. The length of liver in both groups was sharply increased from 6 to 8 months old and then the increase was steady, while the length in the group 2 at the age of 12 months was significantly longer (p<0.01) than that in the group 1. The results indicate that the most organs examined showed the higher development in the feeding level 2 than in the feeding level 1 until the age before 18 months when the steers in the feeding level group 1 were given the compound feed ad libitum.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.5
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• pp.460-470
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• 1997

On an absolute straw diet, the effect of graded levels of green grass supplementation on intake, nutrient digestibility, rumen fermentation pattern and microbial N yield has been studied in cattle. Of the two trials conducted, 16 intact growing bulls of 304 kg weight and 32 months old, randomly allocated to four treatments in a completely randomized design in the 1st trial. While, in the, 2nd trial, four rumen cannulated local bulls of about 60 months old and 400 kg weight were used in a $4{\times}4$ Latin square design with four treatments in four periods. In both the trials, in addition to a mineral mixture, animals were supplemented with graded levels of naturally grown green grass of 0 kg (T1), 2 kg (T2), 4 kg (T3) or 6 kg (T4) to an ad libitum rice straw diet In the 1st trial, measurements were made on intake digestibility, growth rate, N balance and microbial N yield. While in the 2nd trial, in addition to the above parameters (except growth rate), rumen parameters were also studied. All levels of grass supplementation decreased the straw DM intake and increased the substitution rate. The rumen $NH_3-N$ concentration increased with the increase in grass level and ranges from 8-46 mg/l. The rumen pH and the rate and extent of DM degradability of straw were not affeceted by different rumen environments created by different levels of grass inclusion. At 48 h, straw DM degradability were 42, 44, 44 and 43% respectively for 0, 2, 4 and 6 kg grass supplementation daily. The whole gut digestibilities of DM, OM and ADF increased significantly (p < 0.05) only at 6 kg level daily. The microbial N yield was not affected by the levels of grass supplemented. The mean microbial N yield was 10 (SD 3.7) g/kg DOM apparently fermented in the rumen. The estimated minimum N loss and thus the maintenance requirement of tissue protein was 303 mg/kg $W^{0.75}/d$. All the animals lost live weight but 6 kg grass supplementation gave positive energy and N balances. Small amount of green grass supplementation is often recommended for optimization of rumen environment of a straw diet However, under the present experimental condition, no such beneficiary effect observed up to 6 kg (26% of DM intake) level of supplementation.

• Journal of Animal Environmental Science
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• v.1 no.1
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• pp.55-64
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• 1995

A wastewater purifying system using phototrophic bacterium, Rhodopseudomonas capsulata, is currently in operation in several countries, One of them, is a continuously aerated bioreactor(CABR) system, which treats concentrated swin wasterwater using small amounts of phototrophic bacteria as additive bacterial seeding. Using this plant, total biochemical oxygen demand was decreased to 13%, and most of volatile fatty acids were removed. About 40% of the wastewater(Influx) was evaporated during aerobic digestion for 24h, and 60% of that erupted in a decodorized foam(Efflux). The efflux had enough nutrients, N, P and K kor growing plant, as well as organic matters. When the efflux was applied to Italian ryegrass with high dose, fresh shoot and root weights were significantly greater, and $NO_3-N$ contents of the dried shoot were lower than those of control plant (CDU). These results indicate that the CABR plant is useful for reduction and deodorization of swine wastewater and the efflux from CABR can be used for crop production as an organic fertilizer.

• Journal of Adhesion and Interface
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• v.19 no.3
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• pp.101-105
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• 2018

With the rapidly growing of the population and industrization of cities, the clean and affordable water resources have gained immense interest because of remaining about 780 million people still lack access to it. However, present wastewater treatment systems have been faced with various issues, such as low processing efficiency, high operational costs and the requirement of a large area for manufacturing. It is therefore urgently required to develop an inexpensive and efficient wastewater treatment system. As the one of these efforts, we suggested and successfully demonstrated the wastewater treatment system using and electrochemical method via a dimensionally stable anode (DSA) based on rutile type $RuO_2$. Our system achieved biochemical oxygen demand (BOD), chemical oxygen demand (COD), and total organic carbon (TOC) removal efficiently at the respective rates of 52.0%, 77.8%, and 65.6% from household wastewater. In addition, we were able to remove BOD, COD, total nitrogen (TN), and total phosphorus (TP) from animal husbandry wastewater at rates of 92.9%, 75.6%, 35.1%, and 100%, respectively, thereby achieving dramatic reductions. Considering the excellent removal efficiency and the small size of this device, electrochemical wastewater treatment using a DSA coated in rutile $RuO_2$ presents a promising option for the treatment of both household and animal husbandry wastewater.

• Journal of Environmental Impact Assessment
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• v.24 no.5
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• pp.532-548
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• 2015

The winter ecology of individual yellow-throated martens(Martes flavigula) intemperate region of Korea were studied through snow-tracking. The study was performed across 3 winter seasons, from January 2011 to February 2013. Total distance of 49.8km was snow tracked (comprising 13 snow-tracking routes) to determine winter foraging habits, general behavior and movement paths of solitary and small groups (1-6 individuals; $mean=2.9{\pm}1.6$) of yellow-throated martens. The martens in the current study were omnivorous, with their winter diet including 9 animal and 5 plant species. Yellow-throated martens searched for food near and under the fallen logs and branches, root plates of fallen trees, around the roots of growing trees, and in small holes in the ground. They also climbed trees to search inside the tree holes and vacant bird nests. Foraging activity was estimated to occur at a frequency of 1.20 times/km, while territory marking occurred 1.42 times/km on average. Of the 60 documented foraging activities we observed, 17 were successful (28.3%). Moving activity and territory marking mainly occurred along ridges, whereas foraging activity was recorded in valleys, slopes, and forest edges. To protect the habitat of this species, the entire forest should be preserved, including the valleys, slopes, and even forest edges as well as main ridges.