• Journal of Plant Biotechnology
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• 제49권3호
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• pp.222-229
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• 2022

사과는 국내 과수산업에서 가장 많이 재배되고 있는 과종이다. 하지만 apple mosaic virus (ApMV), apple stem grooving capillovirus (ASGV), apple stem pitting virus (ASPV), apple chlorotic leaf spot virus (ACLSV), apple scar skin viroid (ASSVd)와 같은 바이러스 및 바이로이드에 감염되면 과실의 수확량 감소 및 품질 저하를 야기시킨다. 본 연구에서는 국내 사과 농가에서 가장 많이 감염되어 있는 ASGV 바이러스를 제거하기 위한 효율적인 무병화 시스템을 확립하고자 하였다. ASGV에 감염된 폿트묘를 36℃, 38℃, 40℃가 유지되는 항온·항습장치에서 4주간 열처리를 수행하였으며, 신초 생장율과 바이러스 제거율을 조사하였다. 신초 생장률은 36℃ 처리구에서 가장 높았으며 신초의 중간부와 상단부는 바이러스가 제거되었으나 하단부는 바이러스가 제거되지 않았다. 38℃, 40℃ 처리구는 신초의 모든 구간에서 바이러스가 제거되지 않았으며, 40℃ 처리구는 신초의 생장 없이 열처리 3주 후 고사되었다. 36℃ 온도에서 열처리된 폿트묘의 경정을 절취하여 기외에서 접목하였으며 94%의 생존율과 20%의 바이러스 제거율을 보였다. 따라서 열처리 및 경정접목을 통해 무병묘 생산이 가능할 것으로 판단되었다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2007년도 춘계학술대회 논문집
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• pp.66-66
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• 2007

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2009년도 추계학술대회 논문집
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• pp.212-212
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• 2009

Nano composite sample were irradiated and annealed to study irradiation induced phase transformation behavior by nano indention technique. The specimens with 3mm in diameter were irradiatied. Polycrystalline nano filaments were broken their shape and tended to be equilibrium shape like facted sphere. The hardness of irradiatied nanocomposites was decreased by annealing which resulted from the grooving of silver filaments.

• The Plant Pathology Journal
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• 제17권6호
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• pp.368.1-368
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• 2001

• 소성∙가공
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• 제9권4호
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• pp.421-427
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• 2000

The present study has focused on the development of shear textures during groove pressing in an aluminum alloy sheet. The shear components 23 and 13 developed during the groove pressing process. The process consisting of two steps of grooving and flattening each effectively gave rise to a high shear deformation In the sheet without reduction in thickness. The main texture component obtained from the process was the rotated Bs-orientation. The evolution of shear components during the groove pressing caused an increase in R-value of aluminum sheet comparing to a normally processed rolled sheet.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.122-123
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• 2003

Apple stem grooving virus (ASGV) belongs to Capillovirus and infects pome fruits. Transmission mode of ASGV is known by grafting and mechanical inoculation into susceptible hosts, not by any other natural vectors. But we have observed the spread of ASGV in the field without mechanical inoculation or grafting. Transmission seems to be occurred from tree-to-tree and tree-to-susceptible herbaceous plants along but not across ditches in the field. In order to ascertain this possibility, various fungi were isolated and cultured from ASGV-infected plants and 69 isolates were characterized. By means of RNA dot-blot hybridization and PCR analysis, 3 isolates were sorted out for further studies. The isolates were identified to Tataromyces sp. and belonged to Phenicillium by morphological characteristics and molecular markers. As an experimental host, 10 kidney beans (Phaseolus vulgaris) were screened and Kyunggi-5 was selected for virus amplification and symptom development. Kyunggj-5 infected by fungi which seemed to carry ASGV showed the typical disease symptoms and viral coat protein genes were detected from all tested plants. To confirm the Koch's rule, fungi cultured from inoculation origins of kidney bean were grown on PDA media and re-inoculated to hosts. The fungi isolated from inoculation origins induced the typical disease symptoms on hosts. However virus free fungi did not induce any symptom on the experimental hosts. This bioassay showed that these typical symptoms were caused by virus, not fungi.

• The Plant Pathology Journal
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• 제20권2호
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• pp.142-146
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• 2004

Woody plant tissues contain great amounts of phenolic compounds and polysaccharides. These substances inhibit the activation of reverse transcriptase and/or Taq polymerase in RT-PCR. The commonly used multiple-step protocols using several additives to diminish polyphenolic compounds during nucleic acid extraction are time consuming and laborious. In this study, sodium sulfite was evaluated as an additive for nucleic acid extraction from woody plants and the efficiency of RT-PCR assay of commercial nucleic acid extraction kits and small-scale dsRNA extraction was compared. Sodium sulfite was used as an inhibitor against polyphenolic oxidases and its effects were compared in RNA extraction by commercial extraction kit and small-scale double-stranded RNA (dsRNA) extraction method for RT-PCR. During nucleic acid extraction, addition of 0.5%-1.5%(w/v) of sodium sulfite to lysis buffer or STE buffer resulted in lighter browning by oxidation than extracts without sodium sulfite and improved the RT-PCR detection. When commercial RNA extraction kit was used, optimal concentrations of sodium sulfite were variable according to the tested plant. However, with dsRNA as RT-PCR template, sodium sulfite 1.5% in STE buffer improved the detection efficiency of Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in fruit trees, and reduced the unspecific amplifications signi-ficantly. Furthermore, when viruses existed at low titers in host plant, small-scale dsRNA extractions were very reliable.

• Tribology and Lubricants
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• 제11권4호
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• pp.21-27
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• 1995

The effects of interface boundary strength on wear and wear transition during sliding have been investigated in silicon carbide ceramics. Three different microstructures, i.e., solid state sintered silicon carbide, liquid phase sintered silicon carbide and liquid phase sintered silicon carbide composite reinforced with TiB$_{2}$ particulates, were designed by hot pressing. Examinations of crack patterns and fracture modes indicated that interface boundaries were relatively strong between silicon carbide grains in the solid state sintered silicon carbide, intermediate in the liquid phase sintered silicon carbide and weak between silicon carbide grains and TiB$_{2}$ particles in the composite. Wear data and examinations of worn surfaces revealed that the wear behavior of these silicon carbide ceramics could be significantly affected by the interface strength. In the solid state sintered silicon carbide, the wear occurred by a grooving process. In the liquid phase sintered silicon carbide and composite, on the other hand, an abrupt transition in wear mechanism from initial grooving to grain pull-out process occurred during the test. The transition occurred significantly earlier in the composite than in the carbide.

• Journal of Plant Biotechnology
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• 제43권3호
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• pp.391-396
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• 2016

본 연구는 Apple stem grooving virus (ASGV)에 감염된 배 (Pyrus pyrifolia L.) '만수'의 기내배양 식물체를 이용하여 효과적인 배 바이러스 무병화 기술을 알아보고자 수행하였다. 식물체의 경정조직을 잘라 열처리($37^{\circ}C$), 한냉처리($4^{\circ}C$), 항바이러스제인 ribavirin을 단독 또는 복합처리하였다. 처리기간은 2, 4, 8주였으며 ribavirin 농도는 20과 $40mg{\cdot}L^{-1}$이었다. 바이러스 제거율은 Reverse transcription polymerase chain reaction 분석으로 확인하였다. 신초 생존율은 2주간 한냉처리, 항바이러스제 단독처리와 한냉처리와 항바이러스제가 복합처리된 그룹에서 100%로 높았고 열처리에서 33.3%로 가장 낮았다. 단독으로 ribavirin을 처리한 그룹은 처리기간이 길수록 신초 생존율이 감소하는 경향이었다. ASGV 제거율은 2주간 ribavirin $40mg{\cdot}L^{-1}$ 농도로 처리한 그룹과 열처리와 ribavirin을 복합처리한 그룹에서 100%로 높았다. 한냉처리한 그룹의 바이러스 제거율은 16.7%로 가장 낮았으나 한냉처리와 ribavirin을 복합처리한 그룹에서는 43.3%로 향상되었다. 모든 처리에서 처리기간이 길수록 바이러스 제거율은 증가하였다. 본 실험을 통해 배 ASGV 제거에는 경정배양과 더불어 항바이러스제인 ribavirin을 $20mg{\cdot}L^{-1}$ 농도로 4주간 처리하거나 $40mg{\cdot}L^{-1}$ 농도로 2주간 단독처리만으로도 효과적으로 무병화가 가능할 것으로 판단되었다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2004년도 하계학술대회 논문집 C
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• pp.2011-2013
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• 2004

본 논문은 그루빙(grooving),타이닝(tinning),텍스쳐(texture) 등의 포장 도로의 표면 상태를 고성능의 레이저 변위센서를 사용하여 정밀하게 측정하고, 측정한 표면 상태를 객관적으로 평가하는 장비 개발에 관한 논문이다. 본 논문에서는 실제 평가 장비 차량을 만들기에 앞서 전체 시스템을 설계하고, 실내에서 차량과 도로의 모사 장비를 만들어 실험하였다. 본 실험에서는 오실로스코프와 DAQ (Data Aquisition) 보드를 사용하였다. LabView로 선호처리 프로그래밍을 하여 컴퓨터 화면에 GUI 형태로 나타내었다. 측정 데이터는 컴퓨터의 저장 공간에 저장하여 후처리를 가능하게 하였다. 또한, 실제 평가 장비 차량에 장착하게 될, GPS(Global Positioning System) 시스템으로 부터 실시간으로 평가 장비 차량의 이동거리 데이터를 얻었다. 실험 결과 차량이 80km/h로 주행할 때 도로 표면 타이닝의 폭과 깊이가 평균 9.67% 오차를 보였으며, 이동거리는 0.03% 이내의 오차를 보여 만족할 만한 결과를 얻었다.