• Korean Journal of Weed Science
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• v.14 no.3
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• pp.176-183
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• 1994

This study was conducted to investigate how KC6361, a new type diphenylether compound inducing bleaching, increase the greening of cucumber cotyledon in darkness. Protoporphyrin IX formation, reaccumulation rate of protochlorophyllide(Pchlide) in darkness after phototransformation and Shibata shift were not affected. Whereas, aminolevulinic acid(ALA), protochlorophyll and chlorophyll were increased, and especially protochlorophyll was significantly accumulated. When KC6361 and phytol were applied alone or in combination with ALA, the transformation from Pchlide into protochlorophyll was accelerated in darkness. These results suggest that the greening of the etiolated cucumber cotyledon treated with KC6361 seems to be caused by the accumulation of phytol or/and Geranylgeranylpyrophosphate and their increased esterification with Pchlide in darkness.

• Korean Journal of Weed Science
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• v.10 no.3
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• pp.214-220
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• 1990

To establish a greening assay for screening, and physiological and biochemical studies of the compounds affecting biosynthesis of plant pigments, were conducted on environmental factors, and on ways of incubation and illumination which affect plant greening. Greening was good when both cucumber and barley were grown for 5 to 6 days at $25^{\circ}C$ in darkness, when adaxial sides of cucumber cotyledons were contacted with the solution, and when barley leaf fragments were taken 0.5 to 2.0cm from the leaf tip. Potassium phosphate buffer(pH 6.0) at 10mM was most desirable for plant greening. The speed of greening during illumination was increased as the temperature increased from $15^{\circ}C$ to $35^{\circ}C$. The responses were sensitive more in cucumber than in barley, and in chlorophyll biosynthesis than in carotenoid biosythesis. The content of chlorophyll was greatest at the light intensity of 5000 and 1000 lux for cucumber and barley, respectively, but the biosynthesis of carotenoids were greatest at the light intensity higher than for chlorophyll. In use of solvents for dissolving chemicals, acetone, ethyl alcohol and DMSO at 10, 0.1 and 2.5% or less, respectively, did not affect the biosynthesis of plant pigments. $pI_{50}$ values were calculated for chemicals affecting pigment biosynthesis.

• Korean Journal of Plant Resources
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• v.22 no.2
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• pp.136-144
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• 2009

Germination tests on 3 species that have potential for use as sprout vegetables, such as Coreopsis tinctoria, Saussurea pulchella, and Matricaria recutica, were conducted for 20 days under different temperatures of $15{\sim}30^{\circ}C$, and conditions of light and dark. C. tinctoria showed germination of 83% under $15^{\circ}C$ and dark condition after 4 days, S. pulchella 51.7% under $25^{\circ}C$ and light condition after 20 days, and M. recutica 90.3% under $25^{\circ}C$ and dark condition after 4 days. To investigate optimum plantlet size before greening treatment, seeds germinated were allowed to grow under darkness. The optimum growth of C. tinctoria was obtained under $30^{\circ}C$ after 5 days, S. pulchella under $25^{\circ}C$ after 6 days, and M. recutica under $20^{\circ}C$ after 6 days. Greening treatment resulted in diminished longitudinal growth, but C. tinctoria and S. pulchella showed more vigorous latitudinal growth. Days required before marketing as sprout vegetables were different according to species - Three days of greening was good for C. tinctoria, 2 for S. pulchella, no greening for M. recutica. Generally, tightly sealed containers for minimum water loss were recommended for storage of sprout vegetables after harvest. However, storage methods for each species were different depending on various factors like temperature of storage, presence of ventilation holes of storage packages and forms of marketing. More detailed research for above 3 species is proposed.

• Korean Journal of Plant Resources
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• v.22 no.4
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• pp.304-311
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• 2009

This study was conducted to develop functional sprout vegetables with antioxidant effects using seeds of Arctium lappa. The seeds germinated vigorously under light at $25^{\circ}C$, reaching germination rate of 82% within 4 days. Germinated seeds were placed under darkness at various temperatures to force growth in length, and it was demonatrated that $20^{\circ}C$ was optimum temperature. Greening treatment reduced growth in length, but promoted growth of cotyledons. Harvested A. lappa sprout vegetables maintained freshness longer at $10^{\circ}C$, rather than $4^{\circ}C$. Ventilation holes in storage containers had no effects on storage periods. Antioxidant activity of vegetable that received greening treatment for 1-3 days was investigated, and it was shown that free radical scavenging effects and ferrous ion chelating effects was higher than those of commercially available brocoli, cauliflower, pea and bean sprout. Contents of total polyphenol and flavonoid were also higher, especially by 3 day greening. The longer the treatment, the more the inhibition on peroxidation of linoleic acid. Sprout vegetable of A. lappa had higher antioxidant activity compared with adult plant. In conclusion, sprout vegetable of A. lappa has great potentiality for use as one of sprout vegetables.