• Journal of Microbiology and Biotechnology
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• 제10권1호
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• pp.115-118
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• 2000

Recombinant plasmids harboring a heterologous gene coding for the enhanced green fluorescent protein (EGFP) were transfected and expressed in Drosophila melanogaster S2 cells. A stable transformation of polyclonal cell populations expressing EGFP were isolated after 4 weeks of selection with hygromycin B. The recombinant EFGP expressed in transformed S2 cells consisted of a molecular weight of 27 kDa. EGFP expression was also confirmed by fluorometric measurement. The maximum EGFP concentration was about 9.3 mg/I. The present findings demonstrate not only the successful stable expression of EGFP in Drosophuila was about 9.3 mgI. The present findings demonstrate not only the successful stable expression of EGFP in Drosophila S2 cells, but also the use of EGFP as a reporter to analyze gene expression, with its potential of a Drosophila cell expression system for recombinant protein production being an alternative to a baculovirus-insect cell expression system.

• 한국통신학회논문지
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• 제18권1호
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• pp.143-149
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• 1993

An approach is presented for efficient numerical integration of the Sormnerfeld type integrals pertinent to the microstrip surface Green's function arising in the problem of an electric current point source on an infinite planar grounded dielectric substrate. This approach, valid for both lossless and lossy dielectric substrates, is based on the deformation of the integration contour via a coordinate transformation and Cauchy's residue theory, and identifies clearly the effects of surface waves. I ts useful application is in a rigorous moment method analysis of micros trip antenna arrays and microstrip guided wave structures. The efficiency and the usefulness of the present approach are emphasized through some numerical calculations of the impedance matrix elements with associated CPU times.

• International Journal of Advanced Culture Technology
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• 제8권4호
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• pp.101-109
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• 2020

Along with rapid global economic development, people are slowly becoming environmentally aware and green development is increasingly a vital topic of social concern. Academic circles are currently focusing on environmental protection and economic growth to explore the coordinated development of both and to achieve a win-win solution between them. To study the relationship between environmental protection experience and both cost and performance, this study empirically analyzes companies listing on the Shenzhen A-share market from 2014 to 2018. The results show that senior executives' environmental protection experience is negatively correlated with corporate costs, whereas it is positively correlated with business performance. These results provide empirical evidence of the importance of enterprises introducing and cultivating environmental personnel and insisting on sustainable development.

• Journal of Plant Biotechnology
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• 제7권4호
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• pp.211-218
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• 2005

Genetic transformation was carried out by using biolistic gun method. The hypocotyl derived embryogenic calli (explants) of cotton (Gossypium hirsutum L.) cv. Cocker-312 were transformed with a recombinant pGreen II plasmid, in which both, bar (selection marker) and GUS (${\beta}$-glucuronidase) reporter genes were incorporated. Explants were arranged on osmoticum-containing medium (0.5M mannitol) 4 hours prior to and 16 hours after bombardment that was resulted into an increase about >80% for GUS stable expression. 3 days after bombardment, GUS assay was performed, which exhibited, $18.36{\pm}1.00$ calli showed blue spots. The transformed embryogenic calli were cultured on selection medium (@ 6 mg/L basta) for 3 months. The putative transgenic plants were developed via selective somatic embryogenesis (@1.50 mg/L basta); maximum $27.58{\pm}1.25$ somatic embryos were obtained while $17.47{\pm}1.00$ embryos developed into plantlets (@ 0.75mg/L basta). In five independent experiments, up to 7.24% transformation efficiency was recorded. The presence of the transgenes was analyzed by using PCR and southern hybridization analysis. The transgenic plants were developed with in 6-7 months, but mostly transformants were abnormal in morphology.

• 한국의류산업학회지
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• 제9권2호
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• pp.138-144
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• 2007

The purpose of this research was to compare the harmony of tone on tone coloration according to transformation of color area-ratio in shirt and necktie. The respondents were asked to evaluate 48 stimuli of the shirt and necktie with the coloration of the shirt/necktie, the necktie/shirt was separately compounded to be 4 tones such as vivid, light, dull, dark. The subjects were 288 female undergraduate students. The data was analyzed by using SPSS program. Analysis methods were ANOVA, LSD test and t-test. The results of each can be summerized as follows. As to the red, combination of v-lt made a difference in the harmony of coloration according to the transformation of color area-ratio. As to the blue, combination of v-lt, v-dk, lt-d made a difference in the harmony of coloration according to the transformation of color area-ratio. As to the purple, v-lt, v-d was evaluated as harmonized coloration regardless of area-ratio by both male and female students. As to the green, v-lt, lt-d was evaluated as harmonized coloration regardless of area-ratio by both male and female students.

• Journal of Microbiology
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• 제43권4호
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• pp.361-365
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• 2005

In this study, we chronicle the establishment of a novel transformation system for the unicellular marine green alga, Dunaliella salina. We introduced the CaMV35S promoter-GUS construct into D. salina with a PDS1000/He micro-particle bombardment system. Forty eight h after transformation, via histochemical staining, we observed the transient expression of GUS in D. salina cells which had been bombarded under rupture-disc pressures of 450 psi and 900 psi. We observed no GUS activity in either the negative or the blank controls. Our findings indicated that the micro-particle bombardment method constituted a feasible approach to the genetic transformation of D. salina. We also conducted tests of the cells' sensitivity to seven antibiotics and one herbicide, and our results suggested that 20 ${\mu}g$/ ml of Basta could inhibit cell growth completely. The bar gene, which encodes for phosphinothricin acetyltransferase and confers herbicide tolerance, was introduced into the cells via the above established method. The results of PCR and PCR-Southern blot analyses indicated that the gene was successfully integrated into the genome of the transformants.

• Applied Biological Chemistry
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• 제27권
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• pp.56-67
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• 1984

As in many other country, the use of organic matter in Korea has long history. Farmers understand the value of organic matter as the source of plant nutrient and soil improving agent in general. Since 50 years ago, the sources of organic matter in paddy soils were compost, rice and barly straw, green manure, animal waste, fish and beancake, etc.. Application of green manures such as vetch and chinese milk vetch showed no significant effect on the yield of brown rice in paddy soil. On the other hand, the effects of compost and rice straw showed more significant on the yield of brown rice in paddy soil. Application of rice straw in rice cultivation is commonly made at different times between harvest, early spring and several weeks before transplanting. Considering the suitable paddy soil for application of rice straw under well to moderately well drained soil, the yield was pronounced more than poorly drained soil. Based on laboratory and field experimants, application of rice straw promoted the decrease of oxidation-reduction potential in well to moderately well drained soil. This results to be enhanced the release of some mineral nutrients,. such as potassium, calcium, silicon, and increase of availability of soil phosphorus. In the field experiments, results obtained from nitrogen fraction on the immobilization-mineralization of the tracer nitrogen applied in paddy soil,the amount and index of organic nitrogen incoporated in soil was more pronounced in rice straw application than control. Rice straw and its transformation products incoporated in the soil, provided the inflow of energy necessary to maintain heterotrophic microbes activities. Rice straw and its transformation products, especially soluble carbohydrate, enhanced the population of free-living heterotrophic $N_2$ - fixing microbes. Moreover, rice straw and its transformation products in paddy soil, enhanced the activities of soil enzymes such as dehydrogenase and urease.

• 대한기계학회논문집B
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• 제35권6호
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• pp.603-608
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• 2011

입자추적유속계(PTV)는 나노 및 바이오 분야의 유체유동장에서는 각 입자들을 추적하여 속도측정을 하는 관계로 많은 강점이 있다. 그러나 측정원리상 보간에 의한 속도장 측정오차를 피할 수 없는 관계로 PTV기술을 사용함에 있어서 제한적이었다. 본 연구에서는 어파인변환 알고리듬을 PIV 및 PTV측정에 도입함으로써 보간에 의한 오차를 줄일 수 있는 어파인변환 기반 하이브리드 PIV알고리듬을 구축하였다. 구축된 알고리듬에 대한 성능평가를 위하여 Green-Taylor와유동의 수치적 데이터를 이용한 가상영상에 대한 시험을 실시하였으며, 이로부터 입자수가 2000개 이상일 때 최적의 측정성능임을 확인하였으며 상호상관PIV법 및 확률일치PTV법보다 우수한 측정성능임을 확인하였다. 나아가 길이비 2:1($6cm\;{\times}3cm$)인 장방형 물체후류(Re=5,300)에 대한 실험영상에 대한 실제 계산을 통하여 구축된 알고리듬에 대한 측정성능의 우수성을 확인하였다.

• 한국측량학회지
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• 제7권2호
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• pp.85-92
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• 1989

본 연구에서는 원격탐사 기법을 이용하여 인공위성 MSS 데이타와 TM 데이타를 몇 가지의 선형변환된 데이타로 변형시킴으로 분류 정확도향상과 특정 대상물에서의 유효 변환데이타 조합을 알아내고자 하는 것이 주된 목적이라 하겠다. LANDSAT 데이타를 처리함에 있어서, 문제점 중의 하나가 자료의 방대함이며, 이 방대한 자료에 대하여 보다 효율적이고 경제적인 분석을 행하기 위한 방법이 선형변환이다. 이 방법은 여러가지 선형적 산술과 통계적 변환을 하여 다파장 데이타들을 변환시킴으로써, (1) 복잡한 데이타에 대해서는 단순함을 제공 (2) 중복 데이타에 대한 선택적 처리 및 불필요한 자료 제거 (3) 연구대상에 대한 강조등을 행한다. 본 연구에서는 Band Ratioing과 PCA를 수행하여 자료를 변환 분석하여 보았다. 분류 결과 Infrared/RED Ratio는 식물의 특성을 확장시켜 다른 분류 항목과 구별하여 분류하는 데 유용하였으며, 주성분 분석 결과 녹색식물역의 분류에 있어서 Band 1,27이 효과적이었다.

• 대한토목학회논문집
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• 제43권5호
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• pp.675-687
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• 2023

한반도 비무장지대에 인접한 접경지역은 DMZ(DemilitarizedZone) 그린평화 지대화를 위한 그린데탕트(GreenDétent) 실천무대로 주목 받고있다. 본논문은 접경지역에 계획된 생태·환경 분야 산업·인프라 사업의 복합적 성격을 판단 할 수있는 항목으로 구성된 프로슈밍지표(Prosuming-index)를 제안하고, 이 를 통해 그린데탕트 실천 대상지로서의 잠재력과 방향성을 시각화 하였다. 그 결과, 접경 지역중 인천·강화 지역은 '물류·인적 네트워크 중심 권역이자 특화 농수산물 생산지' 로서, 경기 북부지역은 '스마트기술 활용 산업을 기반으로하는 교류 네트워크 중심권역' 으로서, 강원 서부지역과 경기 연천 지역은 '탄소 감축 기술을 골자로 하는 생산-소비 복합권역' 으로서, 강원 동부지역은 '재생에너지 산업을 통한 유휴·노후 인프라의 그린 인프라 전환권역' 으로서, 크게 4가지 권역으로 그린데탕트 실천 잠재력을 유형화 할 수 있었다.