• Journal of fish pathology
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• v.24 no.3
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• pp.297-305
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• 2011

A trap identification system for isolating functional sequences to allow the constitutive expression of foreign protein from Edwardsiella tarda was developed. Using the green fluorescent protein (GFP) reporter-based trap system, various functional sequences to drive heterologous expression of the GFP were selectable in Escherichia coli host. However from the bioinformatic sequence analysis, all the segments predicted as regulatory regions were not native promoters actually existing upstream of endogenous E. tarda genes. Instead, a number of non-authentic sequences, possibly resulted from the random shuffling and/or intermolecular ligation were also proven to be able to display a potent GFP expression in the recombinant E. coli. Further analysis with selected clones showed that both authentic and non-authentic sequences could function in as a constitutive promoter, leading quite a consistent and stable GFP expression after repetitive subcultures. Microscopic examination also confirmed the uniform pattern of GFP expression in every host bacterium. Semi-quantitative assay of GFP showed that there was no clear relationship between expression levels and organizational features of the promoters trapped. Functional promoter-like elements achieved in the present study could be a good starting material for multivalent genetic engineering of E. tarda in order to produce recombinant vaccines in a cost-effective fashion.

• Journal of the Korean Society of Costume
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• v.57 no.2 s.111
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• pp.100-114
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• 2007

This study reports the dress style of Daesarye ceremony participants in the Yeong-Jo Era. The King Yeong-jo attending the archery practice ceremony wears Ikseonkwan(翼善冠), Golryongpo(袞龍袍), Okdae(玉帶), and Heukpihwa(黑皮靴) while royal family members and officers in the ceremony wear Heukdalryeong(黑團領). Each officer in Sangbok(常服) who shoots arrows wears a ring and a wristlet with an archery case on his waist. King's bodyguards wear Yungbok(戎服) or Sangbok(常服). Especially, Muyaebyeolgam(武藝別監) wears Hong-geon(紅巾) and a green Jikryeong(直領). Yujiang-gun(儀位軍) wear Pirip(皮笠) and Hongjuryi(紅紬衣) while Hyeopryeongun(狹輦軍) and Hyeopryeogun(狹輦軍) wear Hong-geon(紅巾), Hongyui((紅衣), Cheonghaengjeon(靑行纏), Hakchang(鶴雲), Hongmokdae(紅木帶), and Unhye(雲鞋). Gyeonmabae(牽馬陪) wear Hwangchorip(黃草笠) and Hwangyui(黃衣). Seupjeongun(拾箭軍) and Hwaekja(獲子) wear Jageon(紫巾), Jayui(紫衣), and Unhye(雲鞋). Jeonak(典樂) wears Mora- bokdu and Noksayui(綠抄衣), while Ak-kong(樂工) wear Hwahwabokdu, Hongjuyui(紅紬衣), Ojeongdae, and Heukpihwa(黑皮靴). Lastly, Hyeopryulrang(協律郎) wears Heukdalryeong(黑團領).

• Journal of the Korean Society of Costume
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• v.59 no.9
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• pp.87-96
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• 2009

Dangui is a formal dress of the wives of high officers and royalty in Chosun Dynasty and it was worn as a day dress in palace and a semi-ceremonial dress in upper-class households. Among the remains of Dangui a double-layered Dangui was found in which two sets of Dangui were combined to be worn as a single piece. The double-layered Dangui consists of an Over-Dangui and an Under-Dangui. The results of the examination of the remains for the Over-Dangui are as follows. As lone as the colors of the remains are identifiable, all of the Over-Dangui were green, whereas the Under-Dangui and lining materials were red. The textile was mostly in woven silk with pattern and it was frequently decorated with golden wefts or foils. A breast embroidery with phoenix and a dragon embroidery were also found. Such Dangui was worn mostly by the high members of the king's palace including the queen, crown princess, bastard princess, and monarch. There were two exceptions of wearing Dangui outside the king's palace. A simple Under-Dangui was made to enable a comfort fit of the two sets of Dangui and the suture was partially sewn. With three doth strings and cuffs, two pieces of Dangui were eventually assembled into one piece of clothing. Differences between the remains and the documents were also found in regards to textile and wearers of Dangui.

• Korean Journal of Human Ecology
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• v.16 no.5
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• pp.1007-1019
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• 2007

The purpose of this study is to investigate the effect of achromatic shirt and chromatic tie combination on image of menswear. This experiment was designed by shirt color(N9, N7, N4, N2), tie color(red, orange, yellow, green, blue, purple), tie tone(vivid, light, dull, dark), and perceiver gender(a male, a female). The experimental materials developed for this study were a set of stimulus and response scales. The stimuli were 96 upper body photographs and 26 7-point bi-polar adjectives were used to evaluate the image. The subjects of this research were 480 male and 480 female college students. The data was analyzed by using SPSS program. Analysis methods were four-way ANOVA. The items of the adjectives were classified into 4 image factor: potency, activity, attractiveness, and visibility. Shirt color, tie color, tie tone influenced on the 4 image dimension greatly by interaction as well as independently. Dull or dark tone tie had an effect on the formation of potency image. And white color shirt, vivid or light tone tie had an effect on the formation of activity image. White or black shirt had a positive effect on attractiveness, and black shirt or vivid tone tie was effective for visible image.

• Journal of the Korean Society of Costume
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• v.54 no.5
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• pp.27-39
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• 2004

Since around 1990. some experimental and shocking ugliness images have been expressed in Fashion and Make-up as well as Fine arts. The purpose of this study is to investigate the trends of ugliness images expressed in Modern Make-up from 1995 to 2003, especially focusing on color, and to enlarge the expressions through formative elements and to anticipate the prospect of Make-up in the future. Ugliness is the most negative aesthetic value which is lack of beauty. The 20 century art trends representing the ugly shape have been distorted or deformed or destroyed and extremely exaggerated with yellow, red, black, blue, white, green. The image and color of ugliness can be summarized as historical, avant-garde, decadence, de-constructive, humorous, futuristic trend and Most of colors are the achromatic ones like black, white, dark gray and red. blue, dark brown and so on. These colors cause some negative attributes such as fear. anger, death, devil, Pain, bad, ill omen, sorrow, despair and the like. At the turning Point in 21C are to be extended the range of color according to the experimental attempts such as informal, collage, graphic and the forth. Lastly, The characteristics of ugliness images expressed in the color of Modern Make-up have been analyzed into formlessness and inaccuracy and deformation by Karl Rosenkranz＇s theory.

• Journal of the Korean Society of Costume
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• v.63 no.5
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• pp.87-101
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• 2013

This paper researches the system of the official's gongbok(公服) during the reign of emperor Hongwu(洪武帝) in the Ming dynasty, and it is based on DaMingLing(大明令), DaMingJili(大明集禮), HongwuLizhi(洪武禮制), ZhusiZhizhang(諸司職掌), DaMingHuidian(大明 會典), MingTaizuShilu(明太祖實錄), and MingShi(明史). Official's gongbok is consisted of clothes, belt(帶), bokdu, scepter(笏), and black shoes. The system of official's gongbok wa schanged through three stages. First, the system of the Yuan dynasty(元代) was maintained in January, 1368, which was the first year of the emperor's reign. Second, the gongbok system was established in November, 1368. The system stated that the clothes had to be red-colored(赤色) regardless of rank and the belt was divided into okdai(玉帶), seodai (犀帶), gumdai(金帶), and kwangsoeundai (光素銀帶). Third, the system was revised in June, 1391, which was the 24th year of his reign. According to the rank, the clothing colors were divided into scarlet(緋色), blue(靑色), and green(綠色). The belt was divided into okdai, seodai, gumdai, and ogaggai(烏角帶). 'Hongmu26nyunjung(洪武26年定)' recorded on the Wanli's edition(萬曆本) of the DaMingHuidian has the meaning of ZhusiZhizhang regulations.

• Journal of Photoscience
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• v.11 no.3
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• pp.121-127
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• 2004

The${\beta}$-glucosidases occur widely in all living organisms and has in general a tendency to form oligomers of varying numbers of subunits or aggregates, although the functional implications of such diverse oligomerization schemes remain unclear. In particular, the assembly mode of the oat ${\beta}$-glucosidase is very unique in that it multimerizes by linear stacking of a hexameric building block to form long fibrillar multimers. Some structural proteins such as actin and tubulin assemble into long fibrils in a helical fashion and several enzymes such as GroEL and Pyrodictium ATPase functional complexes, 20S proteasome of the archaebacterium Thermoplasma acidophilum, and lutamine synthetase fromblue-green algae, assemble into discrete oligomers upto 4 stacked rings to maintain their enzymatic activities. In particular, oat ${\beta}$-glucosidase exists in vivo as a discrete long fibrillar multimer assembly that is a novel structure for enzyme protein. It is assembled by linear stacking of hollow trimeric units. The fibril has a long central tunnel connecting to the outer medium via regularly distributed side fenestrations. The enzyme active sites are located within the central tunnel and multimerization increases enzyme affinity to the substrates and catalytic efficiency of the enzyme. Although it is suggested that oligomerization may contribute to the enzyme stability and catalytic efficiency of ${\beta}$-glycosidases, the functional implications of such diverse oligomerization schemes remain unclear so far.

• Journal of the Korean Society of Clothing and Textiles
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• v.46 no.6
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• pp.945-962
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• 2022

This study examines the use of traditional patterns by new Hanbok brands. A Saekdong print pattern based on previous research was developed and applied to clothing designs. A total of 488 images of printed products from the seven new Hanbok brands and 219 images from the collections of the National Folk Museum of Korea were analyzed. Traditional patterns accounted for 47.4% of the total printed products of the new Hanbok designs, with the following ratio of use, in descending order: flower patterns, traditional paintings, animals, geometrical designs, Dancheong, text and others, Jogakbo, and Saekdong. Saekdong was found in three brand products, and the color or shape was modified. To develop the Saekdong image, five colors - red, yellow, blue, white, and green - were selected. The ratio of use for each color and the width of each color were determined with reference to previous studies. The average color value was determined through color analysis of the Saekdong collections. A total of seven items were designed for the print pattern, and four items were added for coordination to consist of four styles. This study aims to use the results of this analysis to provide insights into product development using traditional patterns.

• Archives of design research
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• v.25 no.5
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• pp.113-119
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• 2012

Under the paradigm of 'Eco', the core of sustainability management, this study aims to establish both domestic and international markets and secure competitiveness in the global markets through the development of children's furniture composed of recycled and unharmful materials. Currently, in Europe and in the Western regions, the concepts of 'eco-friendly', 'children', and 'DIY' are well-placed in the daily lives of the people. On the other hand, compared to the domestic demand for eco-friendly children's furniture, the essential approach and the qualitative improvements about the subject are being slowly progressed. Particularly, the process of applying eco-friendly materials and finishing materials to the children's furnitures are mostly in a non-existent state. Thus, in this study, simple application of basic eco-friendly materials such as natural woods or imitation of overseas furniture designs were avoided to create transformable eco-friendly one-touch DIY children's furniture for children's growth and development through the application of eco-friendly processes of technology, design, and other stages of the development process. In other words, under the big category of eco-friendly children's furniture, the furniture was developed through an environmentally-friendly process of universal design that is suitable for children of all ages, and thus, ultimately maximizing the economical effective value and reducing consumption of resources and environmental pollutions.

• Journal of the Korean Society of Costume
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• v.4
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• pp.13-24
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• 1981

The color yellow was considered from ancient time to the Chosun Dynasty as the central color. Thus, this color became the royal color for the costumes in the palace. It is generally known to usthat the color yellow was controled in use both for the general public and in the royal palaces. However, in the later part of Chosun Dynasty, the color yellow was used not only for the king's costumes but also used for women's tops and for the linings of clothing. Especially, in some of the costumes that belonged to the later period of the Chosun Dynasty, we can still see lots of bright yellow tops. Also there are many green dyed official robes and various costumes for women. It is a true fact that people could not derive the color green from the plants as they did with yellow. The only way they could make the color green was to mix indigo and yellow together. By repeating the difficult process of making various dyes constantly during many centuries, the Korean people developed the marvelous technique of making natural color. Those plants used to make the color yellow are ; Gardenia, Phellodendron amurense, Turmeric, Coptis, Safflower, Arthraxon hispidus, Styphnolobium japonicum. While synthetic dye causes pollution, natural coloring by plants is as safe and useful as the color itself is lovely. Yet it is tragic to know that this traditional culture of making beautiful natural colors was cut off. There is no way to know today the traditionally correct method to derive colors from the plants. Therefore, it is our aim and challenge to find out the original way to dye and develop it and preserve it as our non-polluted folk art. In regard to natural dyeing, we must say that is very difficult to prepare and preserve natural dyes. In the first place, people had to get the right plants at an appropriate time. Then they could not keep those plants too long. Finally, much depended upon the mordant as well as various conditions and dyeing procedures. All those things influenced greatly the quality of color, some times producing a very pretty color and other times a very dull one. It is very appropriate that the natural dye art should be recognized and appreciated anew by Korea since it provides satisfaction to historical and folk artistic demands as well as to those of fashion conscious modern society for high quality consumption items. We propose two stages of development. The first stage is to explore native dye plants and encourage their cultivation. The second stage is to extract from the plants desirable dye which will enhance national culture.