The essential oil was extracted by steam distillation from the aerial part of erect bur-marigold (Bidens tripartita L.), one of the noxious weed in paddy field. The composition of the essential oil was analyzed by gas chromatography-mass spectrometry. The fragrance of the essential oil was green, herbal, oily, spicy. There were 42 constituents in the essential oil:17 hydrocarbons, 6 alcohols, 6 acetates, 5 N-containing compounds, 3 ethers, 3 ketones, 1 lactone and 1 S-containing compound. Major constituents were ${\alpha}$-phellandrene (22.50%), ${\alpha}$-pinene (22.21%), 2,4-dimethyl (2,5-dimethylphenyl) methyl ester benzoic acid (15.11%), limonene (10.66%), ${\beta}$-pinene (35.43%), and ${\beta}$-cubebene (5.27%). The $IC_{50}$ value in MTT assay using HaCaT keratinocyte cell line was 0.018%. However, attachment of patch with 0.1% of the erect bur-marigold essential oil for 24 hr did not show any skin toxicity. Overall results of this study suggest that the essential oil of erect bur-marigold could be used as a source for the development of perfumery industrial products.
Kim, Min Jeong;Jung, U Suk;Jeon, Seung Woo;Lee, Jae Sung;Kim, Won Seob;Lee, Sang Bum;Kim, Youn Chil;Kim, Bae Young;Wang, Tao;Lee, Hong Gu
Asian-Australasian Journal of Animal Sciences
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v.29
no.12
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pp.1734-1741
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2016
This study was conducted to examine the effects of adding phytoncide oil extracted from Korean pine nut cone by-product to the diet of dairy cows on milk yield and compositions, fatty acid characteristics, complete blood count and stress response. A total of 74 Holstein cows were used for 30 days and divided into two groups. Each group was given a basal diet (C) or an experimental diet containing phytoncide additives at 0.016% (T) in feed. The results showed that phytoncide feeding had no effect on milk yield. In addition, there were no observed effects on milk composition, but the ratio of fatty acid in milk was significantly affected by the phytoncide diet, and it showed a positive effect. Not only were the major functional fatty acids, conjugated linoleic acid and eicosapentaenoic acid increased, but also ${\omega}6:{\omega}3$ fatty acid ratio was reduced in milk of T group (p<0.05). In blood analysis, the complete blood count showed no significant difference between C and T group on all parameters. However, the cortisol concentration was significantly decreased in T group compared to control (p<0.05). Taken together, we suggest that phytoncide oil does not have a great influence on the physiological changes, but can be a potential feed additive that improves the milk fatty acid and stress resilience in dairy cows. In addition, it will contribute to the development of feed resource, a reduction in feed cost and a lessening of environmental pollution.
To analyze hydrologic processes in a watershed requires both various geographical data and hydrological time series data. Recently, not only geographical data such as DEM(Digital Elevation Model) and hydrologic thematic map but also hydrological time series from numerical weather prediction and rainfall radar have been provided as grid data, and there are studies on hydrologic analysis using these grid data. In this study, GRM(Grid based Rainfall-runoff Model) which is physically-based distributed rainfall-runoff model has been developed to simulate short term rainfall-runoff process effectively using these grid data. Kinematic wave equation is used to simulate overland flow and channel flow, and Green-Ampt model is used to simulate infiltration process. Governing equation is discretized by finite volume method. TDMA(TriDiagonal Matrix Algorithm) is applied to solve systems of linear equations, and Newton-Raphson iteration method is applied to solve non-linear term. Developed model was applied to simplified hypothetical watersheds to examine model reasonability with the results from $Vflo^{TM}$. It was applied to Wicheon watershed for verification, and the applicability to real site was examined, and simulation results showed good agreement with measured hydrographs.
A green-house experiment was conducted to investigate the growth of Chinese cabbage, radish, and soybean and changes in soil chemical properties after application of 5 and 10mm of pH 2.7 simulated acid rain(SAR) followed by 0, 5, and 10mm of pH 6.0 normal water at the three-day intervals 20 times for Chinese cabbage and radish and 42 times for soybean. The results obtained are summarized as follows: 1. Visual damages by SAR were white-yellow leaf spots, dark brown or light green leaf color, and wrinkled leaf margins in all crops. The degree of visual damages was severer at 10mm than at 5mm SAR and it was reduced as the amounts of normal water increased after SAR application. 2. Chlorophyll content was higher at 10mm than at 5mm SAR application and increased as the amounts of normal water increased after SAR application in all crops. 3. Fresh weights of Chinese cabbage heads and radish roots and grain yield of soybean were higher at 10mm than at 5mm SAR, while they increased as the amounts of normal water increased at the same SAR level. 4. Changes in the mineral contents of plants were not consistent, while S content in radish and soybean increased as the amounts of SAR increased. 5. SAR lowered soil pH, while it increased soil N and S contents. Contents of soil organic matter, P, and exchangeable Ca, Mg, and K were not affected by the amounts of SAR.
In this study, we was measured the radiance reflectance by using multi-spectral image of low resolution camera(LRC) which will be loaded in the multi-purpose satellite(KOMPSAT) to use the data in analyzing water pollution. Also we investigated the possibility of extraction of water quality factors in rivers and water body by using high resolution remote sensing data such as Airborne MSS. Especially, we tried to extract the environmental factors related with eutrophication, and also tried to develop the process technique and the radiance feature of reflectance related with eutrophication. The results were summarized as follows: First, the spectrum of sun's rays which reaches the surface of the earth was consistent with visible rays bands of 0.4${\mu}{\textrm}{m}$~0.7${\mu}{\textrm}{m}$ and about 50% of total quantity of radiation were there. And at around 0.5${\mu}{\textrm}{m}$ of green spectral band in visible rays bands, the spectrum was highest. Second, as a result of the radiance reflectance Chlorophyll-a represented high spectral reflectance mainly around 0.52${\mu}{\textrm}{m}$ of green spectral band, and suspended sediments and turbidity represented high spectral reflectance at 0.8${\mu}{\textrm}{m}$ and at 0.57${\mu}{\textrm}{m}$ each. Third, as a result of the water quality analysis by using Airborne MSS, Chlorophyll-a could have a distribution chart when carried out ratio of B3 and BS to B7. And Band 7 was useful for making the distribution chart of suspended sediments. And when we carried out PCA, suspended sediments and turbidity had distributions at PC 1 , PC 4 each similarly to ground truth data. Above results can be changed according to the change of season and time. Therefore, in order to analyze more exactly the environmental factors of water quality by using LRC data, we need to investigate constantly the ground truth data and the radiance feature of reflectance of water body. Afterward in this study, we will constantly analyze the radiance feature of the surface of water in water body by measuring the on-the-spot radiance reflectance and using low resolution satellite image(SeaWiFs). Besides, we will gather the data of water quality analysis in water body and analyze the pattern of water pollution.
This study, multispectral and hyperspectral sensors were utilized to use radiometric cross validation for the purpose of radiometric quality evaluation of a 'KOMPSAT-3'. Images of EO-1 Hyperion and Landsat-8 OLI sensors taken in PICS site were used. 2 sections that have 2 different types of ground coverage respectively were selected as the site of cross validation based on aerial hyperspectral sensor and TOA Reflectance. As a result of comparison between the TOA reflectance figures of KOMPSAT-3, EO-1 Hyperion and CASI-1500, the difference was roughly 4%. It is considered that it satisfies the radiological quality standard when the difference of figure of reflectance in a comparison to the other satellites is found within 5%. The difference in Blue, Green, Red band was approximately 3% as a comparison result of TOA reflectance. However the figure was relatively low in NIR band in a comparison to Landsat-8. It is thought that the relatively low reflectance is because there is a difference of band passes in NIR band of 2 sensors and in a case of KOMPSAT-3 sensor, a section of 940nm, which shows the strong absorption through water vapor, is included in band pass resulting in comparatively low reflectance. To overcome these conditions, more detailed analysis with the application of rescale method as Spectral Bandwidth Adjustment Factor (SBAF) is required.
The inky cap, Coprinellus congregatus, produces several laccase isozymes during its life cycle: both hyphal tip laccase and sclerotial laccase are involved in the fungal development. When this fungus was transferred to an acid liquid medium (pH 4.0-4.5), a new laccase was synthesized and secreted into the culture supernatant. In order to examine its regulation by external pH, green fluorescent protein gene was ligated at the downstream of the promoters having different lengths. These expression vectors having different promoter lengths were inserted into the fungal transformation vector, pBARGEM7-1. These expression vectors were introduced to the mating type a1 and a2 monokaryons, and the transformants were selected by the phosphinothricin resistance. Transformant a1 (a1TF) and transformant a2 (a2TF) were mated with each other to generate homozygotic dikaryon transformants. All these transformants were grown in neutral liquid medium for 5 days, and then the whole cell homogenates were transferred to the acidic liquid medium (pH 4.1). After 36 h incubation at $25^{\circ}C$, cells were harvested for the analysis of GFP expression. GFP expression was detected in the transformant having full-length promoter (2.0 kb), but other transformants having shorter length promoter (shorter than 1.29 kb) failed to show the fluorescence. Therefore, the acid-responsive element in the laccase promoter should be localized between -2.0 kb ~ -1.29 kb region.
Nam, Myeong-Hyeon;Kim, Hyeon-Suk;Lee, Won-Keun;Gleason, Mark L.;Kim, Hong-Gi
Horticultural Science & Technology
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v.29
no.2
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pp.151-155
/
2011
The fungus Botrytis cinerea causes fruit rot of strawberry and the damages can result in harvest losses upto 50%. Proper timing of fungicide application is essential for successful control of Botrytis fruit rot, fenhexamid plus iminoctadine tris, cyprodinil plus fludioxonil, fludioxonil alone, and Bacillus subtilis QST713 were applied to individual buds, flowers, and green and red fruit of cultivar 'Seolhyang' ex vivo. Cyprodinil plus fludioxonil or fludioxonil alone was applied i) before and after a 5-hr period of low-temperature ($0^{\circ}C$) incubation ex vivo ii) in field trials. Strawberry flowers and red fruit were more susceptible to B. cinerea than the green fruits. Incidence of Botrytis rot with fenhexamid plus iminoctadine tris and cyprodinil plus fludioxonil was the lowest at flowering, whereas B. subtilis QST713 did not significantly among treatments. In 2010, incidence of Botrytis fruit rot was significantly reduced when fludioxonil was applied two times at 1 week intervals from 50% bloom in field trials. Cultivars Redpearl and Seolhyang were more susceptible to low-temperature than cvs. Maehyang and Akihime. Cyprodinil plus fludioxonil application was effective when applied before onset of the low-temperature treatment period. Fludioxonil showed the most effective when it was sprayed one and more than two times in before and post low-temperature condition, respectively. These results demonstrate that fungicide selection and timing can interact with stage of fruit development and low-temperature in determining effectiveness of suppression of Botrytis fruit rot.
Journal of the Korean Institute of Landscape Architecture
/
v.45
no.1
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pp.1-15
/
2017
This study investigated the process of change of 32 urban parks designated during the period of Japanese colonial rule according to growth and development of the city in Busan. Particularly, researching included analyizing books, notification, data, and documents relevant to the history of urban planning published by Busan city. As a result, Daejeong Park, Gokwan Park, and Yongdusan Park had been utilized by Japanese residents under the Japanese colonial rule before planning of urban parks, and 32 parks legally specified in 1944 were planned by considering the prevention against disasters. After emancipation, there were an unauthorized building, housing construction, business district, public office, and school facility in the sites of the parks due to the influence of the Korean War and reorganization of urban planning. The majority of parkways and small parks downtown were eliminated. However, unexecuted parks that the government had designed on the edge of town during the Japanese colonial period have become major parks downtown through the city's growth. Yeonji Park, Yangjeong Park, and Danggok Park have been being building as a business of parks for a comfortable city, forming downtown along with the Green-Busan Policy. Thus, 32 parks designated under the Japanese colonial rule have made or got out of use reflecting on the phases of the times of modern Korean society. It turns out that these parks need an investigation about condition for land possession and purchase of the site of the parks in order for social common capital.
This study is written to bring the proposal forward for the direction of south korean naval force. The political situation and the circumstance of the world, especially in the area of Pacific Ocean, are changing very rapidly. North Korea has been always the conventional existing intimidator for South Korea since the 6·25-War. Additionally, the strengthening movements of the national defense, which is easily noticed from China and Russia, is also an other part of intimidating countries against South Korea. Those three mentioned countries are continually developing the asymmetrical warfare systems, for example a strategic nuclear weapon. Since the Obama Administration, the Asia-Pacific Rebalancing-Strategy has been changed as an East Asian foreign policy. Nowadays, Trump Administration renamed the 'United States Pacific-Command' to 'United States Indo-Pacific Command'. The purpose of this is not only letting India to participate in american alliance, but also reducing an economic burden, which is often mentioned in USA. West Germany was located in the very similar geopolitical position during the Cold War just like South Korea these days. And that's why the strategy of West German Navy is worthy of notice for south korean naval force to decide its suitable strategy. Most of all, the two most important things to refer to this study are the plan to expand naval air force and the realistic political stand for us to take it. In conclusion, I laid an emphasis on maintenance of 'green-water-navy'. instead of selecting the strategy as a 'blue-water-navy'. The reason I would like to say, is that south korean navy is not available to hold the unnecessary war potential, just like Aircraft-Carrier. However, this is not meaning to let the expansion of naval force carelessly. We must search the best solution in order to maintain the firm peace within the situation. To fulfill this concept, it is mostly very important to maintain the stream of laying down a keel of destroyers, submarines and air-defense-missile, as well as the hight-tech software system, taking a survey of 4th industrial revolution. Research and development for the best solution of future aircraft by south korean navy is likewise necessary. Besides, we must also set the international diplomatic flexibly. As well as maintaining the relationship with US Forces, it is also very important to improve the relationship with other potential allied nation.
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