• Journal of the Korean Wood Science and Technology
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• v.46 no.1
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• pp.10-16
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• 2018

Lindera erythrocarpa is a less utilized species in Korea although that it has straight stem and it grows up to 40 cm in diameter. A proper kiln-dry schedule is required in advance to utilize an unknown species. Terazwa's quick oven-dry method was used to find it and which was confirmed by drying 25 mm thick boards in a kiln. The average green moisture content and the average green specific gravity of Lindera erythrocarpa are 72.3% and 0.53, respectively. Prospective kiln-drying conditions obtained by Terazwa's quick oven-dry method are a initial dry-bulb temperature of $50^{\circ}C$, a initial wet-bulb depression of $4^{\circ}C$ and a final dry-bulb temperature of $75^{\circ}C$, which are in a good agreement with USDA FPL kiln-dry schedule of T5-D4. 25 mm thick boards dried in a kiln with T5-D4 kiln-dry schedule did not have any severe drying defects such as honycombing and warping. A severer kiln-dry schedule of T8-C5 was developed and applied to another kiln-drying run to confirm it.

• Korean Journal of Medicinal Crop Science
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• v.3 no.3
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• pp.233-239
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• 1995

The effects of media, growth regulators, low-temperature treatments, culture temperature and light were investigated to improve the callus induction and growth in the anther culture of Comus officinalis Sieb. et Zucco. The frequency of callus induction was more effective on WPM medium than MS medium, and it was highest as 54% in WPM medium supplemented with Img/L NAA. Callus growth was stimulated on MS medium supplemented with 2mg/L NAA. Effect of temperature and light on the callus induction and growth was highest as 62% in the treatment for 16/8 hrs. (light/dark) at $25^{\circ}C$ Ef­fect of low - temperature treatment on callus induction was highest as 19. 5% in the treatment for 36 hrs. at $4^{\circ}C$. For organization, green cells and rootings were promoted on MS medium supplemented with O. 5mg/L 2,4-D and 1 mg/L kinetin. The prevention of callus browning was effective on the medium con­taining $3{\sim}5mg/L$ ABA or 5mg/L $AgNO_3$. The supplement of ABA or $AgNO_3$,were maintained callus ac­tivity for 4-5 weeks and they were promoted the development of green cells.

• Journal of the Korean Association of Geographic Information Studies
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• v.9 no.2
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• pp.20-29
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• 2006

Modeling urban climate caused by land use conversion is critical for human welfare and sustainable development, but has hampered because detailed information on urban characteristics is hard to obtain. With the advantage of satellite observations and the new statistical boundary system, this paper measures the economic and environmental effects of green area loss due to land use conversion in urban areas. To perform this purpose, data were collected from the various sources basic statistical unit data from the National Statistical Office, digital maps from the National Geographic Information Institute, satellite images, and field surveys when necessary. All data (maps and attributes) are built into the geographic information system (GIS). This paper also utilizes Landsat TM 5 imagery of Daegu city to derive vegetation index and to measure average surface temperature. The satellite data were examined using standard image processing software, ERDAS IMAGINE, and the results of the digital processing were presented with ARCVIEW(v.3.3). SAS package was used to perform statistical analyses. This study presents that there exists a strong relationship between land use change and climatic change as well as land price change. Based on results of the analysis, this paper suggests that planners should implement effective tools and policies of urban growth management to detect environmental quality and to make right decisions on policies concerning smart urban growth.

• Journal of the Korean Association of Geographic Information Studies
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• v.10 no.1
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• pp.169-182
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• 2007

The purpose of this study is to assess the urban amenity on physical environmental factors, and to verify the effectiveness of the amenity map using correlation analysis of the environmental quality. The physical environmental factors were set to 4 items of green space, landscape, water environment and land use, and the items were composed of 11 detail factors. Based on the factors, GIS maps were constructed and the amenity map was produced using overlay analysis. The results of this study are as follow; the proportion of 1~2 ranks was 40.8% of total area around Dalseong-gun, Dong-gu, Suseong-gu. The lower 6~7 ranks in the amenity assessment was 5.7% of total area around Jung-gu, Seo-gu, Dalseo-gu. According to the results of correlation analysis between the amenity and environmental observed data, $SO_2$ among the air pollution material had a negative correlation with amenity(r=-0.649, p<0.05). In case of water pollution, TN and TP had the negative correlations with amenity (r=-0.643, P<0.01; r=0.642, p<0.01).

• Korean journal of applied entomology
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• v.47 no.1
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• pp.31-36
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• 2008

The embryonic and postembryonic developments of Nezara antennata Scott were observed in 5 different rearing cages such as A (Cylindrical, ${\phi}\;10cm{\times}4cm$), B (Cylindrical, ${\phi}\;14.5cm{\times}2.8cm$), C (Rectangle, $6.5L{\times}6.5cmW{\times}10cmH$), D (Cylindrical, ${\phi}$ 9cm in bottom & ${\phi}$ 11.5 cm in $upper{\times}10.8cm$) and E (Cylindrical, ${\phi}\;15cm{\times}7.5cm$) containing soybean and peanut seeds as food, and sponge soaked with water under laboratory condition of $24^{\circ}C$ and 15L : 9D. Hatchability ranged from 93 to 97%. Nymphal duration was shortest of 6 days in the 1st instar and longest of 10 days in the 5th instar. The nymphal duration was 38 to 39 days observed in the rearing cages. Emergence rate was in the range from 53 to 62% with highest in A and B cages. Adult longevity was 65 to 75 days for male, and 67 to 74 days for female, and was longest in the B cage. Total number of eggs laid by female adult was in the range from 51 to 56 without significant difference in the rearing cages, and was the most in the B cage. Accordingly, the reproductive rate of N. antennata for 1 generation was within 25 to 33 times, and was highest in the B rearing cage. Therefore, it could be concluded that B cage is most suitable for stable rearing of N. antennata under laboratory condition.

• The Journal of Korean Society of Virology
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• v.28 no.1
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• pp.31-38
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• 1998

In Korea, all domestic made test systems for detecting antibodies in HIV-1 contain the antigens from human immunodeficiency type 1 (HIV-1) subtype B. However, because HIV-1 subtype O is significantly different in amino acid sequences from all other subtypes of HIV-1, there has been a need for developing a test for detecting antibodies in subtype O. For this purpose, the entire nucleotide sequence corresponding to the extracellular domain of the transmembrane glycoprotein of HIV-1 subtype O was synthesized with consideration of Escherichia coli condon usage. Various regions of the extracellular domain were cloned into E. coli expression vectors and tested for levels of protein production. The nucleotide sequence, named ECTM, that can encode a 129 amino acid-long peptide, was found to be expressed at a high level in E. coli. The protein of approximately 17 kDa specifically reacted with sera from individuals infected with HIV-1 subtype O. The ECTM protein was purified to near homogeneity by the CM-T gel chromatography, using concentrated, denatured inclusion bodies. In Western blot analysis, the purified viral antigen reacted with sera from individuals infected with subtype O more efficiently than subtype B. The enzyme linked immunoabsorbent assay (ELISA) system was developed using the subtype O viral protein and compared with the commercially available kit lacking the antigens from subtype O. The ELISA kit containing the subtype O antigen ECTM alone efficiently reacted with sera from individuals infected with subtype O. The subtype O antigen-containing kit produced a positive absorbence even when sera were diluted 512-fold, suggesting a high sensitivity. The commercially available kit also reacted with subtype O sera, but produced a negative result at a dilution of 8-fold. Our results suggest that the currently available kit may not be able to efficiently detect subtype O sera and that the viral protein developed in this study may be added to the current system to maximize the detection of sera from individuals infected with subtype O.

• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.123-132
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• 2009

This study was designed to investigate the effect of the late fall fertilization applied with methyl urea(MU), compound chemical fertilizer(CF), humate(HM), and organic compost fertilizer(NS) on spring greenup of creeping bentgrass at following year. The plots were treated with various snow cover periods before transforming to ski slopes from golf holes during 2007 fall to 2008 spring. The highest visual quality and greenup rate were shown on MU or HM applications at 10 days before snow cover treatment. The CF treatment which had a highest phosphorus rate was most effective with a 13 cm of root length at the reconversion date to golf hole from ski slope of the following spring. However, the application of CF followed by immediate snow cover showed the worst results on visual quality and green color caused by a leaf burning damage from the residual effect of CF. At least 10 days were required to avoid phytotoxicant from undissolved granular of CF before snow cover practise. The application of NS showed the highest result on leaf dry weight at no snow cover plot in next spring, but not on green color and visual quality. Therefore, the proper interval period of snow cover after late fall fertilization should be an important management skill on the spring greenup of creeping bentgrass on following year transforming from ski slope to golf hole.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.587-594
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• 2000

At present embryonic stem (ES) cells with confirmed pluripotential properties are only available in the mouse. Recently, we were able to isolate, culture and genetically transform primordial germ cell (PGC)-derived cells from pig embryos and demonstrate their ability to contribute to chimera development in the pig. In order to determine whether the system we developed could be used to isolate embryonic germ (EG) cells from other mammalian species, we placed isolated PGCs from cattle, goats, rabbits and rats in culture. Briefly, PGCs were isolated from fetuses of cow (day 30-50), goat (day 25), rabbit (day 15-18) and rat (day 11-12), and plated on STO feeder cells in Dulbecco's modified Eagle's medium (DMEM): Ham's F10 medium (1:1) supplemented with 0.01 mM nonessential amino acids, 2 mM L-glutamine, 0.1 mM $\beta$ - mercaptoethnol, soluble recombinant human stem cell factor (SCF; 40ng/ml), human basic fibroblast growth factor (bFGF; 20ng/ml) and human leukemia inhibitory factor (LIF; 20ng/ml). For maintenance of the cells, colonies were passed to fresh feeders every 7-10 days. In all species tested, we were able to obtain and maintain colonies with ES-like morphology. Their developmental potential was tested by alkaline phosphatase (AP) staining and in vitro differentiation assay. For genetic transformation, cells were electroporated with a construct containing the green fluorescent protein (GFP) under the control of the cytomegalovirus (CMV) promoter. GFP-expressing colonies were detected in cattle, rabbits and rats. These results suggest that PGC-derived cells from cattle, goats, rabbits and rats can be isolated, cultured, and genetically transformed, and provide the basis for analyzing their developmental potential and their possible use for the precise genetic modification of these species.

• Korean journal of applied entomology
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• v.52 no.3
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• pp.243-248
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• 2013

This study was conducted to understand the settlement process of Ascotis selenaria larvae into citrus orchards with respect to oviposition site and analysis of the spatial distribution pattern of the larvae. A. selenaria eggs were not found on citrus trees in field and green house, but not on citrus trees in the field. A. selenaria larvae showed a significant clump distribution in the greenhouse. In the open citrus field, the index of dispersion was around 1.0 in most cases, with a weak clumping degree. However, the d-statistic was between -1.96 and 1.96, indicating a statistically significant random distribution. In addition, the Green's index (a clumping index) was very low in all cases, even though the clump distribution was accepted. for most samples, the probability distribution of larval frequency in the field satisfied the probability distribution functions of Poisson (random pattern) and the negative binomial (clump pattern) distribution. In addition, the temporal distribution of the larvae in the open field showed a pattern which was formed by colonizers from outside oviposition sites. Further, the difference in larval spatial distribution between field and greenhouse orchards was discussed.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.3
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• pp.231-241
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• 2020

In this study in order to develop new approaches we investigated using high voltage pulsed electric fields (PEF) technology to reduce the risks, protect the phyto-constituents and improve skin biological activities. After preparing a Chaga mushroom (Inonotus obliquus) extracts pretreated with PEF, components measurement and skin efficacy evaluation were performed. As a result of the content measurement, the content of polysaccharide and polyphenol were higher in the order of extracts treated with 50 Hz and 25 Hz at 0.5 kV/cm, and the content of protein was the highest in extracts treated with 25 Hz at 0.5k V/cm. Similar to the results of the polyphenol measurements, extracts treated with 25 Hz and 50 Hz at 0.5 kV/cm showed leading DPPH scavenging ability. The cell protection effect against sodium dodecyl sulfate (SDS) and UVB was finest in extracts treated with 25 Hz at 0.5 kV/cm, which had the highest protein content. And the hyaluronic acid synthesis was leading in extracts treated with 50 Hz and 100 Hz at 0.5 kV/cm. Therefore, the active ingredient of the high-voltage PEF pre-treatment Chaga mushroom extract can be developed as a functional material with cell protection and moisturizing effect, and such green technology is expected to be used in various fields of cosmetics and material development.