• Journal of The Korean Society of Agricultural Engineers
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• v.62 no.2
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• pp.83-95
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• 2020

The water circulation in agricultural watersheds changes with the operation of agricultural reservoirs, it is necessary to classify and evaluate them into upstream, agricultural reservoirs, irrigation districts, and downstream. Therefore, in this study, we developed the agricultural water circulation rate (AWCR) considering an agricultural reservoir and irrigation district by improving the water circulation rate of the Water environmental conservation Act. we applied it to Jinwi watershed using the module-based hydrologic analysis system to simulate the water circulation for agricultural reservoirs and irrigation areas. The model performance during the validation period was NSE of 0.762 for the downstream stream and 0.682 for the reservoir level. And the hydrograph separation model was applied to separate the direct and baseflow. As a result of this study, The AWCR of Jinwi watershed was 71.8% on average, which was higher than the water circulation rate estimated by the downstream hydrograph separation.

• Journal of Korean Society of Disaster and Security
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• v.6 no.3
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• pp.19-27
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• 2013

Along with green growth era in the 21st century and with the increasing economic, social, and cultural stabilization, people expect absolute freedom from the drought and flood and long for ecological exchange in the waterfront as a part of the living base at the same time. In order to match the changes in social demand, the focus on the river management policy urgently requires a comprehensive river management that considers the nature's environmental and ecological aspects as well as a new disaster prevention approach that grows out of existing simple flood prevention. Therefore, this thesis provides feasible solutions by suggesting the following findings to maintain and manage rivers to be harmonized with the natural environment considering disaster prevention aspects. It is necessary to change the river management policy and integrated river basin management, to control the river management lead by the central government and to promote close-to-nature river management approach for environmentally sound and sustainable development.

• Microbiology and Biotechnology Letters
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• v.10 no.1
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• pp.33-37
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• 1982

The work has been carried out for the development of antifungal antibiotics possessing curative effect in the control of sheath blight disease of rice plant. Soil samples were collected from over 1600 spots throughout the country. More than 1300 specimens which seem to be the genus Streptomyces were isolated from the soil samples. Screening procedures consist of respective processes by four steps. Those are growth inhibition test in liquid culture, paper disk method, dendroid test and green house test. 102 isolates appeared to be active against Pellicularia sasakii when all specimens isolated were examined by the first growth inhibition test. Finally a strain of Streptomyces forming strong antifungal substances against P. sasakii was selected from a soil sample of Mt. Soyo, Kyeongi Province. Antifungal substances formed by the strain were isolated and purified from the culture broth and examined for antimicrobial activities as to be specific against fungi but not active on bacterial growth.

• Journal of the Korean Applied Science and Technology
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• v.29 no.3
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• pp.503-515
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• 2012

${\alpha},{\beta}$-Dichlorosuccinic acid was synthesized through the reaction of maleic anhydride with $Cl_2$(g) and ultra violet(200~300nm) wavelength in the presence of $CCl_4$. The second reaction of N-(${\alpha},{\beta}$-dichloro)succinic acid contained glucosamine derivatives(I) was accomplished by a modification of the general acylation using excess ${\alpha},{\beta}$-dichlorosuccinic anhydride in the presence of 2% acetic acid with methanol as a solvent at elevated temperature($70^{\circ}C$). We considered organic acid derivatives were useful especially of treatment for the cultivating porphyra and skin beauty culture.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.6
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• pp.545-554
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• 2009

This study was conducted to develop and characterize of a high quality salted mackerel using enzymatic extracts of Ecklonia cava (EEC). In this study, potential antioxidative properties of EEC were evaluated by DPPH free radical scavenging activity, hydrogen peroxide scavenging activity, peroxide value, and fatty acid composition, and the antimicrobial properties were also measured by analysis for volatile basic nitrogen, pH, viable cells, Eschericia coli and biogenic amine. Compared to EEC-untreated salted mackerel, the salted mackerel with EEC was superior in antioxidative properties, while was negligible in the difference of antimicrobial properties. These results suggested that the high quality salted mackerel with antioxidative activity could be developed by treatment of EEC.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.3
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• pp.977-985
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• 2012

Performance optimization of a small engine intake port has been studied through computational and experimental approach. Port angle, flange area and port shape are very important design parameters affecting performance of an intake port. Especially, radius of curvature of intake port inner surface has major effect on the flow coefficient of an intake port. As increasing port angle and flange area, flow coefficient is increased because pressure distribution and pressure gradient in the intake port are improved. Even though computational results over-predict maximum 8% compared with experimental result, they describe the tendency of flow coefficient according to the design parameters. Optimized intake port shows about 4.5% improved flow performance.

• Journal of The Korean Society of Agricultural Engineers
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• v.58 no.1
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• pp.81-90
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• 2016

The Agricultural Systems Application Platform (ASAP) provides bottom-up modelling and simulation environment for agricultural engineer. The purpose of this study is to expand usability of the ASAP to the second order partial differential equations: elliptic equations, parabolic equations, and hyperbolic equations. The ASAP is a general-purpose simulation tool which express natural phenomenon with capsulized independent components to simplify implementation and maintenance. To use the ASAP in continuous problems, it is necessary to solve partial differential equations. This study shows usage of the ASAP in elliptic problem, parabolic problem, and hyperbolic problem, and solves of static heat problem, heat transfer problem, and wave problem as examples. The example problems are solved with the ASAP and Finite Difference method (FDM) for verification. The ASAP shows identical results to FDM. These applications are useful to simulate the engineering problem including equilibrium, diffusion and wave problem.

• Journal of Microbiology and Biotechnology
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• v.16 no.6
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• pp.952-960
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• 2006

As a unicellular green alga that possesses many of the metabolic pathways present in higher plants, Chlorelia offers many advantages for expression of heterologous proteins. Since strong and constitutive promoters are necessary for efficient expression in heterologous expression systems, the development of such promoters for use in the Chlorella system was the aim of this study. Proteins encoded by the early genes of algal viruses are expressed before viral replication, probably by the host transcriptional machinery, and the promoters of these genes might be useful for heterologous expression in Chlorella. In this study, putative promoter regions of DNA polymerase, ATP-dependent DNA ligase, and chitinase genes were amplified from eight Korean Chlorella virus isolates by using primer sets designed based on the sequence of the genome of PBCV-1, the prototype of the Phycodnaviridae. These putative promoter regions were found to contain several cis-acting elements for transcription factors, including the TATA, CAAT, NTBBF1, GATA, and CCAAT boxes. The amplified promoter regions were placed into Chlorella transformation vectors containing a green fluorescence protein (GFP) reporter gene and the Sh ble gene for phleomycin resistance. C. vulgaris protoplasts were transformed and then selected with phleomycin. The GFP fluorescence intensities of cells transformed with chitinase, DNA polymerase, and DNA ligase gene promoter-GFP fusion constructs were 101.5, 100.8, and 95.8%, respectively, of that of CaMV 35S-GFP-transformed Chlorella cells. These results demonstrate that these viral promoters are active in transformed Chlorella.

• Journal of Microbiology and Biotechnology
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• v.17 no.7
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• pp.1213-1216
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• 2007

The vector pCW5 with plasmid pC7, originally isolated in Lactobacillus paraplantarum C7 derived from kimchi, was constructed using a p32 strong promoter, the pC7 replicon, and green fluorescent protein (GFP) as the reporter. The constructed vector was transformed into E. coli and Leuconostoc mesenteroides, and GFP expression detected using a Western blot analysis. GFP fluorescence was recognized in E. coli and Leuconostoc mesenteroides using a confocal microscope. In addition, GFP fluorescence was also clearly detected in several industrially important lactic acid bacteria (LAB), including Lactobacillus bulgaricus, Lactobacillus paraplantarum, and Lactobacillus plantarum. Thus, pCW5 was shown to be effective for Leuconostoc mesenteroides when using GFP as the reporter, and it can also be used as a broad-host-range vector for other lactic acid bacteria.

• Journal of Welding and Joining
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• v.33 no.6
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• pp.27-35
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• 2015

Laser welding sector in the automotive industry has been widely recognized as one of the most important bonding processes, such as parts welding. Efforts to improve productivity and weld quality have been progressing steadily. In addition, laser welding is suitable for welding process that can produce high-quality welds suitable for flexible production and small quantity batch productions. In order to ensure the rigidity of the material, high strength material are applied to more than 1 GPa class body parts and automotive bumper beams. However, not only the situation is that the trend of domestic research, but also development is based on product molding considering freedom of shape where reinforcement is applied to meet the safety regulations and high-speed crash performance, despite the use of high strength materials. The tendency for heat-affected zone (HAZ) softening phenomenon common in areas of laser welded high tensile steel welding confirmed the occurrence of weld softening effect according to the process parameters. Based on this, range of process parameters could be selected for ensuring weld quality.