• Journal of Plant Biotechnology
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• v.29 no.4
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• pp.223-228
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• 2002

We have developed a homemade particle inflow gun (PIG) system which has simple operation method, low price and high gene introduction efficiency into rice callus. Rice callus were inflowed with gold particles containing DNA of a plasmid, pIG121Hm, harboring intron GUS ($\beta$-glucuronidase) gene, NPTII gene and HPT gene. For optimal GUS transient expression, the effects of parameters on DNA delivery efficiency of the PIG system was investigated by scoring transient GUS expression. The highest number of blue spots was observed at 16 mM of spemidine and 1.5 M of calcium chloride, respectively. And the amount of gold particles required for the best GUD expression was 2 mg. Optimum GUS transient expression was observed at target distance of 12 cm and helium pressure of 3.5 bar (50 psi). Gene introduction efficiency of the PIG system was observed almost similar to that of the Biolistic Gun (Bio-Rad Company). Since PIG system is simple to operate and one doesn't need disposable accessaries, the PIG system can be easily applied to various replication experiments.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.4
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• pp.315-320
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• 1987

The survey was carried out in the Goryeong area in Gyeongbug province to point out the hinderances of grave increment on the development of hilly to mountainous area and to give basic data to improve the entombment custom. The total extent of gravesite in the area was mapped 148ha which were 0.4% of total area and 1.8% of arableland respectively. Scattering of graves might be hindered utilization of the mountainous area. Out of the total gravesite, about 60% was situated on the areas of 30-60% slopes, and the occupation extent in average was about $89m^2$ per grave which was wider than that of the national average $52m^2$. The soils occupied by gravas ware characterizod by having recommendable properties about 73% for grassland, about 17% for woodland and 8% for orchards.

• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.1
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• pp.71-81
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• 1989

Soil erosion was investigated to find out difference in amount of soil eroded from slope land at early stage of young grasses and at later stage with sufficient cover with different slopes. The six experimental plots were formed on 8$^{\circ}$, 10$^{\circ}$, 15$^{\circ}$, 20$^{\circ}$, and 25$^{\circ}$, with 2m width and 20m length located at the Hwak Kok Ri, Chun Sung Gun, Kang Weon Do. The amount of soil eroded and run-off were collected from 1. May 1987. to 30. October 1988, growing with grasses sowed 2. September 1987. The results were as follows : 1. The amount of soil eroded from the plots except 8$^{\circ}$ plot exceeded the allowable soil erosion with 14 ton/ha during the land formuing before establishment of sufficient surface cover with grasses. Therefore, proper soil conservation practice should be recommeneed. 2. The amount of soil eroded increased exponentially with increased slope as 1.24 times for 15$^{\circ}$1.65 times for 20*, and 2.94 times for 25$^{\circ}$, m comparing with standared 10$^{\circ}$ polt. 3. The erosion occurred mainly by high density of rainfall exceeding lOOmm as consecutive precipitation during the raining peried or accompanied by typhoon passing. 4. The significant soil erosion, when the land covering ratio was over 95% after seeding of grass, was recorded only by the single continuous storms over lOOmm of concentrated precpitation, of which amounts were 1/73~/250 of the allowable soil erosion. 5. The amount of soil erosion from the plots with sufficient surface cover with grasses increased as the slope increased however the amounts were small enough to be neglected. 6. Desolation by soil erosion would be minor problem up to the slope of 20$^{\circ}$ when the mountainous area developed to the grassland with sufficient cover. But it could be concerned on the turn to the hare land by the treading of livestocks with the land slope over 25$^{\circ}$. 7. The run-off of rainfall increased by the increament of slope but it was not exponentially increased. 8. The run-off of rainfall after seeding of grass reduced by 20% in comparison with the run-off of rainfall before seeding, which might be due to infiltration of rainfall promoted by the grass roots.

• Journal of the Korean association of regional geographers
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• v.17 no.4
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• pp.493-504
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• 2011

Based on 3-period land use data using RS and GIS, this paper analyzed landuse change and subsequent loss of ecosystem service value, using the ecosystem service value coefficients put forward by Costanza. Results show that from 1917 to 2006, area of crop land, plantation, building site, unused land increased and natural forest, grassland, water area, wetland decreased, in Musan region and Hoeryeung region. But the area of natural forest is acute reduced by 7,925.34ha and the amplitude of building site is increased rapidly in Musan region. The area of grass land is acute reduced by 71,594.34ha and the amplitude of unused land is increased rapidly in Hoeryeung region. Total ecosystem services value of Musan region reduced dramatically, from $66,135.26{\times}10^6$ won to $37,894.51{\times}10^6$ won in period 1917~2006. The net decreased ecosystem services value is $28,240.75{\times}10^6$ won and the increased amplitude is 42.71% in Musan region. Total ecosystem services value of Hoeryeung region reduced dramatically, from $145,862.69{\times}10^6$ won to $106,948.44{\times}10^6$ won in period 1917~2006. The net decreased ecosystem services value is $38,914.25{\times}10^6$ won and the increased amplitude is 26.68% in Hoeryeung region. Therefore, the effective measures should be taken to maintain sustainable development of environment.

• Korean Journal of Environmental Biology
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• v.32 no.4
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• pp.363-370
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• 2014

Various ecosystem carry out fundamental function of material circulation and energy flow through interrelationship with many environmental factors. Therefore, it is crucial to scientifically understand the value of nature to deduce correlation between environmental factor and change of ecosystem function. In this study, we determined the accumulated ecosystem carbon and characteristics of soil respiration on grassland vegetation in Namahangang basin in Namhangang Basin. It was found that the rate of soil respiration was highly correlated with the soil temperature in all communities. The measured soil respiration rates were $1,539mgCO_2\;m^{-2}h^{-1}$, $1,200mgCO_2\;m^{-2}h^{-1}$, $1,215mgCO_2\;m^{-2}h^{-1}$ in Miscanthus sacchariflorus, Phragmites japonica, Salix koreensis communities, respectively. Also, carbon quantities accumulated in litter and soil layers were $40.6tCha^{-1}$ (1.9+38.7), $46.9tCha^{-1}$ (43.0+3.9), $31.2tCha^{-1}$ (28.9+2.3) in M. sacchariflorus, P. japonica, S. koreensis communities, respectively.

• Journal of Korean Society for Geospatial Information Science
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• v.23 no.1
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• pp.113-121
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• 2015

Remote sensing technology using a multi-spectral satellite imagery can be utilized for the analysis of urban heat island effect in large area. However, weather condition of Korea mostly has a lot of clouds and it makes periodical observation using time-series of satellite images difficult. For this reason, we proposed the analysis of urban heat island effect using time-series of Landsat TM images and ATC model. To analyze vegetation condition and urbanization, NDVI and NDBI were calculated from Landsat images. In addition, land surface temperature was calculated from thermal infrared images to estimate the parameters of ATC model. Furthermore, the parameters of ATC model were compared based on the land cover map created by Korean Ministry of Environment to analyze urban heat island effect relating to the pattern of land use and land cover. As a result of a correlation analysis between calculated spectral indices and parameters of ATC model, MAST had high correlation with NDVI and NDBI (-0.76 and 0.69, respectively) and YAST also had correlation with NDVI and NDBI (-0.53 and 0.42, respectively). By comparing the parameters of ATC model based on land cover map, urban area had higher MAST and YAST than agricultural land and grassland. In particular, residential areas, industrial areas, commercial areas and transportation facilities showed higher MAST than cultural facilities and public facilities. Moreover, residential areas, industrial areas and commercial areas had higher YAST than the other urban areas.

• Proceedings of the KSAR Conference
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• 2001.10a
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• pp.37-43
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• 2001

1. About fifty thousand of cattle embryos were transferred and 16000 ET-calves were born in 1999. Eighty percents of embryos were collected from Japanese Black beef donors and transferred to dairy Holstein heifers and cows. Since 1985, we have achieved in bovine in vitro fertilization using immature oocytes Collected from ovaries of slaughterhouse. Now over 8000 embryos fertilized by Japanese Black bull, as Kitaguni 7 -8 or Mitsufuku, famousbulls as high marbling score of progeny tests were sold to dairy farmers and transferred to their dairy cattle every year. 2. Embryo splitting for identical twins is demonstrated an useful tool to supply a bull for semen collection and a steer for beef performance test. According to the data of Dr.Hashiyada (2001), 296 pairs of split-half-embryos were transferred to recipients and 98 gave births of 112 calves (23 pairs of identical twins and 66 singletons). 3. A blastomere-nuclear-transferred cloned calf was born in 1990 by a joint research with Drs.Tsunoda, National Institute of Animal Industry (NIAI) and Ushijima, Chiba Prefectural Farm Animal Center. The fruits of this technology were applied to the production of a calf from a cell of long-term-cultured inner cell mass (1998, Itoh et al, ZEN-NOH Central Research Institute for Feed and Livestock) and a cloned calf from three-successive-cloning (1997, Tsunoda et al.). According to the survey of MAFF of Japan, over 500 calves were born until this year and a half of them were already brought to the market for beef. 4. After the report of "Dolly", in February 1997, the first somatic cell clone female calves were born in July 1998 as the fruits of the joint research organized by Dr. Tsunoda in Kinki University (Kato et al, 2000). The male calves were born in August and September 1998 by the collaboration with NIAI and Kagoshima Prefecture. Then 244 calves, four pigs and a kid of goat were now born in 36 institutes of Japan. 5. Somatic cell cloning in farm animal production will bring us an effective reproductive method of elite-dairy- cows, super-cows and excellent bulls. The effect of making copy farm animal is also related to the reservation of genetic resources and re-creation of a male bull from a castrated steer of excellent marbling beef. Cloning of genetically modified animals is most promising to making pig organs transplant to people and providing protein drugs in milk of pig, goat and cattle.

• Ecology and Resilient Infrastructure
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• v.6 no.4
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• pp.267-276
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• 2019

The Gonggeomji Reservoir is a historical irrigation facility built in the 8th century and designated as a wetland protected area by Ministry of Environment, Korea. In order to collect the baseline data necessary for developing a sustainable conservation strategy, we investigated the classification of actual vegetation, the vegetation distribution and the floristic structure of the vegetation in the Gonggeomji Wetland Protection Area. In the whole protection area, a total of 26 plant communities were classified including the wetland, riparian, grassland, forest, farmland, and orchard vegetation. According to the results of detrended correspondence analysis, the structure of wetland vegetation was mainly affected by water depth and human disturbance. In reservoir wetlands, floating vegetation such as Utricularia vulgaris var. japonica, Trapa japonica, and emergent vegetation such as Nelumbo nucifera, Typha spp. completely covered the water surface. Since 2014, the reservoir wetland has been terrestrialized with the expansion of emergent and hygrophytic plants. For the sustainable conservation and restoration of wetland protected areas, it is necessary to naturalize the topography and wetland vegetation, recovery the hydrologic system, and restore ecosystem connectivity from wetlands to forests.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.20 no.5
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• pp.1-13
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• 2017

Recently, due to worldwide industralization and urbanization, natural environment has been severly damaged and global warning is worsening. Heat wave, torrential rainfall, typhoon and other natural disasters continuously occur due to global warming. Policies such as carbon emission regulation are taken into effect to solve such problems. Such global trend has affected interest to natural ecosystem and developed as a concept of ecosystem-services. This study particularly focused on ground water storage capacity among various ecosystem-services such as climate control and soil formation. It is because Korea suffers from drought and flood every year. Therefore, this study aims to understand objective numerical value of ecosystem-services value regarding ground water storage capacity of biotop classes based on relationship among precipitation, amount of evapotranspiration, and runoff of 7 regions of Chilgok-gun, Gyeongsangbuk-do and to convert the value into economic value. The study calculated ground water storage capacity based on relationship among precipitation, amount of evapotranspiration, and run off. Calculated amount of each capacity was 29.26 million ton($30.2mm/m^2$), 430.46 million ton($140.4mm/m^2$), 11.30 million ton($150.1.0mm/m^2$), 33 milion ton($3.0mm/m^2$). Economical value of ecosystem-service by each biotop classes appeared 4,128,800 thousand KRW ($21.32KRW/m^2$) for agricultural biotop, and 60,403,600 thousand KRW ($98.52KRW/m^2$) for forest biotop, 1,572,800 thousand KRW ($104.4KRW/m^2$) for grassland biotop, and 47,600 thousand KRW ($2.18KRW/m^2$) for bare ground biotop. The result of this study like above, it will be used as important evidentiary material to preserve natural resource effectively from various development business and policies that damages natural eco-system. Also, it is judged that ecosystem-service value by each land coverage will be used as important material for preparing legalistic institution such as establishing natural environment preservation plan, budget for alternative forest resource creation cost.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.7
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• pp.950-956
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• 2017

Objective: Effects of newly isolated Lactobacillus plantarum on the fermentation and chemical composition of fresh rice straw silage was evaluated in this study. Methods: Lactic acid bacteria (LAB) from good crop silage were screened by growing them in MRS broth and a minimal medium with low carbohydrate content. Selected LAB (LAB 1821) were Gram-positive, rods, catalase negative, and were identified to be Lactobacillus plantarum based on their biochemical characteristics and a 16S rRNA analysis. Fresh rice straw was ensiled with two isolated LAB (1821 and 1841), two commercial inoculants (HM/F and P1132) and no additive as a control. Results: After 2 months of storage at ambient temperature, rice straw silages treated with additives were well-preserved, the pH values and butyric and acetic acid contents were lower, and the lactic acid content and lactic/acetic acid ratio were higher than those in the control (p<0.05). Acidity (pH) was lowest, and lactic acid highest, in 1821-treated silage (p<0.05). The $NH_3-N$ content decreased significantly in inoculant-treated silage (p<0.05) and the $NH_3-N$ content in 1821-treated silage was lowest among the treatments. The dry matter (DM) content of the control silage was lower than that of fresh rice straw (p<0.05), while that of the 1841- and p1174-inoculant-treated silages was significantly higher than that of HM/F-treated silage. Microbial additives did not have any significant (p>0.05) effect on acid detergent fiber or neutral detergent fiber contents. Crude protein (CP) content and in vitro DM digestibility (IVDMD) increased after inoculation of LAB 1821 (p<0.05). Conclusion: LAB 1821 increased the CP, IVDMD, lactic acid content and ratio of lactic acid to acetic acid in rice straw silage and decreased the pH, acetic acid, $NH_3-N$, and butyric acid contents. Therefore, adding LAB 1821 improved the fermentation quality and feed value of rice straw silage.