• Journal of the Korea Institute of Information and Communication Engineering
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• v.23 no.3
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• pp.261-266
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• 2019

Flow cytometry is an electrical detection system that provides precise and diverse optical properties to cells and micro particles. Flow cytometry, which provides multidimensional information including cell size and granularity through light scattering and fluorescence emission generated by the induction of light of a specific wavelength to the fluorescently treated cells or micro particles, plays an important role in biomedical and biophysical fields. However, it has some drawbacks such as high cost, size of the instrument and limitation in selecting fluorescent dyes. Therefore, in this paper, a low cost compact fluorescent detection system is developed using light-emitting diode and microcontroller. The proposed fluorescence detection system has a replaceable the light source/fluorescence filter/photodetector and constructed by 3D printer, so that the user can design a customized system according to the selected fluorescent dyes. The fluorescence intensity was measured by varying the number of fluorescently labeled cells, and the measured intensities showed a high linearity within the tested concentration ranges.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.15 no.9
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• pp.3274-3297
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• 2021

Permission delegation is an important research issue in access control. It allows a user to delegate some of his permissions to others to reduce his workload, or enables others to complete some tasks on his behalf when he is unavailable to do so. As an ideal solution for controlling read access on outsourced data objects on the cloud, Ciphertext-Policy Attribute-Based Encryption (CP-ABE) has attracted much attention. Some existing CP-ABE schemes handle the read permission delegation through the delegation of the user's private key to others. Still, these schemes lack the further consideration of granularity and traceability of the permission delegation. To this end, this article proposes a flexible and fine-grained CP-ABE key delegation approach that supports white-box traceability. In this approach, the key delegator first examines the relations between the data objects, read permission thereof that he intends to delegate, and the attributes associated with the access policies of these data objects. Then he chooses a minimal attribute set from his attributes according to the principle of least privilege. He constructs the delegation key with the minimal attribute set. Thus, we can achieve the shortest delegation key and minimize the time of key delegation under the premise of guaranteeing the delegator's access control requirement. The Key Generation Center (KGC) then embeds the delegatee's identity into the key to trace the route of the delegation key. Our approach prevents the delegatee from combining his existing key with the new delegation key to access unauthorized data objects. Theoretical analysis and test results show that our approach helps the KGC transfer some of its burdensome key generation tasks to regular users (delegators) to accommodate more users.

• Journal of the Korean GEO-environmental Society
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• v.12 no.8
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• pp.13-24
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• 2011

In the part of geotechnical engineering, soils are classified as either the coarse grained soil or the fine-grained soil following the fine content($F_c$=50%) according to the granularity, and appropriate design codes are used respectively to represent their mechanical behaviour. However, sand-clay mixtures, which are typically referred to as intermediate soils, cannot be easily categorized as either sand or clay. In this study, several monotonic undrained shear tests were carried out on Silica sand fine mixtures with various proportions, and a wide range of soil structures, ranging from one with sand dominating the soil structure to one with fines controlling the behaviour, were prepared using compaction method or pre-consoldation methods in prescribed energy. The shear strength of mixtures below the threshold fines content is observed that as the fines content increases, maximum deviator stress ratio decrease for dense samples while an increase is noted for loose samples. Then, by using the concept of fines content and granular void ratio, the monotonic shear strength of the mixtures was estimated. It was found that the shear behavior of mixtures is greatly dependent on the skeleton structure of sand particles.

• Journal of the Korea Society of Computer and Information
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• v.14 no.4
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• pp.87-99
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• 2009

The top-down method for SOA delivery is recommended as a best way to take advantage of SOA. The core step of SOA delivery is the step of service modeling including service analysis and design based on ontology. Most enterprises know that the top-down approach is the best but they are hesitant to employ it because it requires them to invest a great deal of time and money without it showing any immediate results, particularly because they use well-defined component based systems. In this paper, we propose a service identification method to use a well-defined components maximally as a bottom-up approach. We assume that user's inputs generates events on a GUI and the approximate business process can be obtained from concatenating the event paths. We first find the core GUIs which have many outgoing event calls and form event paths by concatenating the event calls between the GUIs. Next, we adapt sequential pattern mining algorithms to find the maximal frequent event paths. As an experiment, we obtained business services with various granularity by applying a cohesion metric to extracted frequent event paths.

• Clinical and Experimental Reproductive Medicine
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• v.50 no.4
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• pp.270-276
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• 2023

Objective: This study investigated the clinical and laboratory factors associated with the presence of dysmorphic oocytes in intracytoplasmic sperm injection (ICSI) cycles. Methods: The study involved 200 ICSI cycles, performed from 2020 to 2021, that yielded at least one mature oocyte. Clinical characteristics and ovarian stimulation methods were compared between 68 cycles with at least one dysmorphic oocyte (the dysmorphic group) and 132 cycles with normal-form oocytes only (the non-dysmorphic group). Dysmorphic oocytes were characterized by dark cytoplasm, cytoplasmic granularity, cytoplasmic vacuoles, refractile bodies in the cytoplasm, smooth endoplasmic reticulum in the cytoplasm, an oval shape, an abnormal zona pellucida, a large perivitelline space, debris in the perivitelline space, or an abnormal polar body. Results: The ages of the women, indications for in vitro fertilization, serum anti-Müllerian hormone levels, and rates of current ovarian endometrioma were similar between the dysmorphic and non-dysmorphic groups. In both groups, the three ovarian stimulation regimens, two types of pituitary suppression, and total gonadotropin dose were employed similarly. However, the dual-trigger method was used more frequently in the dysmorphic group (67.6% vs. 50%, p=0.024). The dysmorphic group contained significantly more immature oocytes and exhibited significantly lower oocyte maturity (50% vs. 66.7%, p=0.001) than the non-dysmorphic cycles. Within the dysmorphic group, significantly lower oocyte maturity was found in the cycles using a dual-trigger, but not in those with a human chorionic gonadotropin trigger. Conclusion: ICSI cycles with dysmorphic oocytes are closely associated with reduced oocyte maturity. This association was observed exclusively in dual-trigger cycles.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.12C
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• pp.1173-1183
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• 2006

In this paper, we propose an efficient method for improving visual quality of AR-FGS (Adaptive Reference FGS) which is adopted as a key scheme for SVC (Scalable Video Coding) or H.264 scalable extension. The standard FGS (Fine Granularity Scalability) adopts AR-FGS that introduces temporal prediction into FGS layer by using a high quality reference signal which is constructed by the weighted average between the base layer reconstructed imageand enhancement reference to improve the coding efficiency in the FGS layer. However, when the enhancement stream is truncated at certain bitstream position in transmission, the rest of the data of the FGS layer will not be available at the FGS decoder. Thus the most noticeable problem of using the enhancement layer in prediction is the degraded visual quality caused by drifting because of the mismatch between the reference frame used by the FGS encoder and that by the decoder. To solve this problem, we exploit the principle of cyclical block coding that is used to encode quantized transform coefficients in a cyclical manner in the FGS layer. Encoding block coefficients in a cyclical manner places 'higher-value' bits earlier in the bitstream. The quantized transform coefficients included in the ealry coding cycle of cyclical block coding have higher probability to be correctly received and decoded than the others included in the later cycle of the cyclical block coding. Therefore, we can minimize visual quality degradation caused by bitstream truncation by adjusting weighting factor to control the contribution of the bitstream produced in each coding cycle of cyclical block coding when constructing the enhancement layer reference frame. It is shown by simulations that the improved AR-FGS scheme outperforms the standard AR-FGS by about 1 dB in maximum in the reconstructed visual quality.

• Journal of the Korean GEO-environmental Society
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• v.13 no.7
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• pp.5-12
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• 2012

Recently the installation of reinforced earth retaining walls in the domestic construction site has increased, surpassing conventional RC walls. These reinforced walls have various types depending on the reinforcing material, installation method and the form of face panel. However, there are difficulties in design and construction management due to the unproved safety of construction method. In case of reinforcing materials, despite the fact that they come in all different sizes and types produced by small businesses or partially imported with cheap price and low quality, no proper standards for designing the walls have been suggested. In order to apply reinforced retaining wall system to broad cases and design the walls effectively considering site conditions, specific design and construction guidelines for efficient construction management are needed. In conclusion, this study verified that reduction factors can be greatly affected by grain sizes and stiffness of backfill materials and granularity range, therefore in case of relatively large construction site, it is required to redesign the reinforced retaining wall by evaluating site installation resistance test, applying respective reduction factors to each backfill material and select the right geogrid depending on the usage of retaining wall so as to enhance the safety of reinforced earth retaining walls with efficiency.

• Journal of the Korea Institute of Building Construction
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• v.19 no.6
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• pp.477-484
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• 2019

The purpose of this study is to present basic data for developing concrete with EMP shielding as the structure materials when constructing an EMP shielded building structure. In order to use metal-based recycled aggregates with excellent conductivity and easy procurement for EMP shielding concrete, an evaluation of the stability evaluation and EMP shielding performance was performed. Through the stability evaluation, it was found that the coarse aggregate stability criterion was satisfied, but the oxidized slag did not satisfy the fine aggregate stability criterion, the oxidized slag is not satisfied. In addition, as a result of fresh concrete, the workability is increased and the air volume is decreased. The compressive strength is increased due to the high density and coarse granularity of the recycled aggregates, which increased the cement paste and adhesion, thereby increasing the compressive strength. The results of an EMP shielding test show that aggregates with high shielding performance are electronic arc furnace(EAF) Oxidizing Slag and Cooper Slag. The shielding performance is expected to increase if the average particle size of aggregate is small or uniformly distributed.

• Journal of Microbiology
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• v.42 no.1
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• pp.25-31
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• 2004

Sf9 cells have obvious advantages for the conventional production technology of vaccine. They are useful tools for high concentration and large-scale cultures. Sf9 cells were grown to maximal concentration, 8${\times}$l0$\^$6/ cells/$m\ell$ in a 500$m\ell$ spinner flask, with a doubling time at the exponentially growing phase of 24.5 hours, using serum-free media. To explore the ability of Sf9 cells to be infected by the Japanese encephalitis (JE) virus Beijing-l strain, Sf9 cells were infected with the virus. By 4-5 days post-infection, 10-15 % of the Sf9 cells showed cytopathic effect (CPE), from granularity to the formation of syncytia and multinucleated giant cells continuously observed over a period of 35 days. Positive fluorescent reactions were detected in 30-40% of cells infected with the JE virus Beijing-l strain, and the uninfected Sf9 cells were completely negative. Virus particles, propagated in Sf9 and Vero cells, were concentrated by sedimentation on 40% trehalose cushions by ultracentrifugation, and showed identical patterns of viral morphogenesis. Complete virus particles, 40 to 50 nm in diameter, were observed, and JE virus envelope (E) proteins, at 53 kDa, were found in the western blot analysis to the anti-JE virus E protein monoclonal antibody and reacted as a magenta band in the same position to the glycoprotein staining. To evaluate whether the infectious virus was produced in Sf9 cells inoculated with the JE virus Beijing-l stain, Sf9 cells were inoculated with the virus, and sample harvested every 5 days. The titers of the JE virus Beijing-l strain rose from 1.0${\times}$l0$\^$5/ to 1.5${\times}$l0$\^$6/ pfu/$m\ell$. The infected Sf9 cells could be subcultured in serum-free medium, with no change in the plaque sizes formed by the JE virus Beijing-l strain in the plaque assay. It is suggested that the ability of the JE virus Beijing-l strain to infect Sf9 cells in serum-free media will provide a useful insect cell system, where the JE virus replication, cytopathogenicity and vaccine immunogen can be studied.

• Journal of KIISE:Databases
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• v.33 no.7
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• pp.754-765
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• 2006

In XML keyword search, a search result is defined as a set of the smallest elements (i.e., least common ancestors) containing all query keywords and a granularity of indexing is an XML element instead of a document. Under the conventional index structure, all least common ancestors produced by the combination of the elements, each of which contains a query keyword, are considered as a search result. In this paper, to avoid unnecessary operations of producing the least common ancestors and reduce query process time, we describe a way to construct a partitioned index composed of several partitions and produce a search result by merging those partitions if necessary. When a search result is restricted to be composed of the least common ancestors whose depths are higher than a given minimum depth, under the proposed partitioned index structure, search systems can reduce the query process time by considering only combinations of the elements belonging to the same partition. Even though the minimum depth is not given or unknown, search systems can obtain a search result with the partitioned index, which requires the same query process time to obtain the search result with non-partitioned index. Our experiment was conducted with the XML documents provided by the DBLP site and INEX2003, and the partitioned index could reduce a substantial amount of query processing time when the minimum depth is given.