• Title/Summary/Keyword: grains

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Electron Microscopic studies on the Ultrastrucure of Pyrenoid and Cell Wall in Chlorella Cells. (Chlorella 세포의 Pyrenoid 와 세포벽구조에 관한 전자현미경적 연구)

  • 이주식
    • Korean Journal of Microbiology
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    • v.4 no.1
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    • pp.1-13
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    • 1966
  • The author examined for observing the structures of pyrenoid and cell wall of three strains of Chlorella ellipsoidea and relation of pyrenoid to starch grain formation at the ultrastructure level. 1. The development of pyrenoid of Chlorella species from the time of its initiation and its subdetail sequent activities are described in some pictures. 2. Close correlation between the findings of light microscopy and electron microscopy is proved. 3. The pyrenoid is a dynamic organellae which continues to change its appearance thoughout the development of the Chlorella cell. 4. The starch grains are continously formed by deposition of carbohydrate within the chloroplast with the aid of pyrenoid factors. 5. Some parental starch grains are passed on the daughter cell during cell division. 6. The Da stage cells contain only chlaroplast without pyrenoid matrix. In Da stage a pyrenoid is surrounded by starch and starch grains appear in chloroplast lamellae. In $L_1L_2$ stages, large starch grains of lens form accumulate in cell. In $L_3$ stage pyrenoid disappears for a time and starch grains are scattered. In cell division stage starch grains are divided into four groups. In $L_4$ stage, pyrenoid substance appears temporarily and disappears soon. At this stage the cell is constituted of Dn cell containing chloroplast only. 7. The cellular boundary of JE strain except Y 815 and Y 511 strain contains 250.angs. intermediate layer of unknown chemical composition between the fibrillar cellulose wall and the out capsule layer.

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Physicochemical Properties of Non-glutinous, Dull, and Glutinous Rice Grain in Segregating Populations of Dull/Glutinous Crosses

  • Kim, Kwang-Ho;Kim, Eun-You;Jeong, Young-Pyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.3
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    • pp.277-281
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    • 1999
  • Dull grains segregated from F$_3$ and F$_4$ of the crosses between two dull mutants and a glutinous cultivar were compared with non-glutinous and glutinous segregants for their physicochemical properties. Amylose content of dull rice grain segregated from the dull/glutinous cross showed the intermediate value between glutinous and non-glutinous rice grain, whether it is controlled by the recessive or dominant gene. Alkali digestibility value (ADV) of dull rice grain was lower than that of glutinous or non-glutinous rice. A positive correlation was found between ADV and amylose content of homozygous non-glutinous or dull F$_4$ grains, but a negative relationship was observed in glutinous grains. Protein content of dull grain was significantly higher than that of glutinous or non-glutinous grain segregated from the same cross, while those of glutinous and non-glutinous grains were not different. Among gelatinization characteristics, initial pasting temperature and peak viscosity of dull grains were higher than glutinous rice, and were not different with non-glutinous grain. Hot, cool and consistency viscosities of dull grain were intermediate between glutinous and non-glutinous rices. Dull grains showed the highest breakdown viscosity and the lowest setback viscosity among the three endosperm types.

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Morphological Features of Pollen Grains in Portulaca

  • Kim, InSun
    • Applied Microscopy
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    • v.43 no.2
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    • pp.75-80
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    • 2013
  • The morphological features of pollen grains collected from the broad-leaved and cylindrical-leaved Portulaca species of the Hawaiian Islands were investigated. The variation in size and surface pattern were examined using scanning electron microscopy and statistical analysis. Pollens of the Portulaca were apolar grains of monads exhibiting apertures, colpi, and relatively thin echini. Of particular interest was the differing size of pollen grains among the species studied. Pollens of the broad-leaved Portulaca were considerable in size with a length of about $73{\sim}86{\mu}m$, while the cylindrical-leaved Portulaca tended to have smaller pollen grains ranging from 50~65 in diameter. The smallest pollens were found in cultivar 2 having an average of $43{\mu}m$. The pollen was intectate, and the exine surface appeared granulous, having sculptured elements of spinules and puncta. Two types of puncta were discerned; one forming an operculum, and the other, a simple perforation. Numerous spinules and small puncta were found throughout the cylindrical-leaved Portulaca. In the present study, morphological features of pollen grains from ten Hawaiian Portulaca species, including endemic and unknown taxa, revealed the aforementioned pattern of variation. This paper aim to provide morphological information that could be of phylogenetic value within the Hawaiian Portulaca.

INFRARED EMISSION FROM SPHERICAL DUST CLOUDS

  • Lee, Hyung-Mok;Hong, Seung-Soo;Yun, Hong-Sik;Lee, Sang-Gak
    • Journal of The Korean Astronomical Society
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    • v.25 no.2
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    • pp.111-128
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    • 1992
  • Infrared emissions from spherical dust, clouds are calculated using quasi-diffusion method. We have employed graphite-silicate mixture with power-law size distribution for the dust model. The grains are assumed to be heated and cooled by radiative processes only. The primary heating source is diffuse interstellar radiation field. hut the cases with an embedded source are also considered. Since graphite grains have higher temperature than silicate grains, the observed IR emission is mainly due to graphite grains, unless the fraction of graphite grains is negligibly small. The color temperature of Bok globules obtained from IRAS 60 and $100{\mu}m$ data are found to be consistent with the dust cloud with graphite-silicate mixture exposed to average interstellar radiation field. The color temperature is sensitive to the external radiation field, but rather insensitive to the size distribution of the grains. We found that the density distribution can be recovered outside the beam size using the inversion technique that assumes negligible optical depth. However, the information within the beam size is lost for if beam convolved intensity distributions are used in deriving density profile.

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Study of the Microbial and Chemical Properties of Goat Milk Kefir Produced by Inoculation with Taiwanese Kefir Grains

  • Chen, Ming-Ju;Liu, Je-Ruei;Lin, Chin-Wen;Yeh, Yu-Tzu
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.5
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    • pp.711-715
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    • 2005
  • One of the prerequisites for the successful implementation of industrial-scale goat kefir production is to understand the effects of different kefir grains and culture conditions on the microbial and chemical properties of the goat kefir. Thus, the objectives of the present study were to evaluate the characteristics of kefir grains in Taiwan on the microbial and chemical properties of goat milk kefir, as well as to understand the influence of culture conditions on production of medium chain-length triglycerides (MCT). Kefir grains were collected from households in northern Taiwan. Heat-treated goat milk was inoculated with 3-5% (V/W) kefir grains incubated at 15, 17.5, 20 or 22.5$^{\circ}C$ for 20 h, and the microflora count, ethanol content, and caproic (C6), caprylic (C8), and capric acid (C10) levels measured at 4 h intervals. Our results indicate that incubation with kefir grains results in 10$^6$-10$^7$ CFU/ml microflora count and 1.18 g/L of ethanol content at 20 h of fermentation. Incubation with 5% kefir grain at 20-22.5$^{\circ}C$ produces the highest MCT levels.

Kinetic Friction of Grains on Surfaces (곡물의 동마찰계수 측정에 관한 연구)

  • 김만수;이동호
    • Journal of Biosystems Engineering
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    • v.1 no.1
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    • pp.49-54
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    • 1976
  • No reliable basic data were available on the kinetic friction coefficients between grains and frictional surfaces being used in grain handling equipments in Korea. In order to determine appropriate kinetic friction coefficient between three grains (Tongil, Jinheung, barley) and two surfaces at four levels of moisture content of grain, the laboratory tests using the newly designed experimental apparatus and strain gage measuring system were carried out, and the relationship among factors that affect kinetic friction coefficients of grains were analyzed. The results of this study are summarized as follows ; 1. Kinetic friction coefficient of samples being tested was dependent on surfaces and moisture content of grains . The ranges of them were 0.369-0.512 for Tongil variety , 0.347-0.469 for Jinheung variety, and 0.360-0.502 for barley, respectively. 2. There was a little difference of kinetic friction coefficients between two varieties of rice at the same testing conditions. Because the interaction had been large among treatments , each value of kinetic friction coefficient determined in this study should be used only for the corresponding conditions in this study . 3. Kinetic friction coefficients in creased linearly with increase in moisture content of grains and their regression equations were shown in Table.4. 4. Kinetic friction coefficients on a PVC surfaces was shown a little lower values than on the steel surfaces at various moisture levels, but especially in Jinheung and barley, a big difference was shown.

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Biocontrol Activity of Volatile-Producing Bacillus megaterium and Pseudomonas protegens against Aspergillus flavus and Aflatoxin Production on Stored Rice Grains

  • Mannaa, Mohamed;Oh, Ji Yeon;Kim, Ki Deok
    • Mycobiology
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    • v.45 no.3
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    • pp.213-219
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    • 2017
  • In our previous study, three bacterial strains, Bacillus megaterium KU143, Microbacterium testaceum KU313, and Pseudomonas protegens AS15, were selected as effective biocontrol agents against Aspergillus flavus on stored rice grains. In this study, we evaluated the inhibitory effects of the volatiles produced by the strains on A. flavus growth and aflatoxin production on stored rice grains. The three strains significantly reduced mycelial growth of A. flavus in dual-culture assays compared with the negative control strain, Sphingomonas aquatilis KU408, and an untreated control. Of these tested strains, volatiles produced by B. megaterium KU143 and P. protegens AS15 markedly inhibited mycelial growth, sporulation, and conidial germination of A. flavus on agar medium and suppressed the fungal populations in rice grains. Moreover, volatiles produced by these two strains significantly reduced aflatoxin production in the rice grains by A. flavus. To our knowledge, this is the first report of the suppression of A. flavus aflatoxin production in rice grains using B. megaterium and P. protegens volatiles.

Measurement of Crystallite Size of Method and Evaluation of Crystal Defects (X선 회절법에 의한 할로겐화 은 유제입자의 크리스탈라이트 크기 측정과 결정결함 평가에 관한 연구)

  • Bae, Chang-Hwan;Lee, Ju-Hee;Han, Chang-Suk
    • Korean Journal of Materials Research
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    • v.19 no.6
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    • pp.330-336
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    • 2009
  • The size of crystallites in mono-dispersed cubic silver bromide grains was measured by applying a powder X-ray diffraction method and Scherrer's equation to grains that were suspended in swollen gelatin layers. In order to evaluate the existence of defects, the measured crystallite size was compared to those measured by using a scanning electron microscope. In the case of the grains prepared by the controlled double jet method, the size of crystallites was equal to the edge length of the grains that had edge lengths smaller than 400 nm. This result proved the usefulness of the above-stated method for measuring the size of crystallites and also evaluating the presence of any crystal defect in each grain. In the case of the grains, which were precipitated in the presence of a sensitizing dye and potassium iodide, the size of crystallites was smaller than the edge's length, indicating the discontinuities in the grains introduced during the precipitation process.

Quality and Physicochemical Characteristics of Rice Cooked along with Various Mixed Grains and by Following Different Cooking Methods (취반방법에 따른 혼합잡곡밥의 품질 및 이화학 특성)

  • Kim, Hyun-Joo;Lee, Ji Hae;Lee, Byong Won;Lee, Yu Young;Lee, Byoung Kyu;Jeon, Yong Hee;Ko, Jee Yeon;Woo, Koan Sik
    • The Korean Journal of Food And Nutrition
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    • v.31 no.5
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    • pp.653-667
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    • 2018
  • This study evaluated the quality characteristics, the presence of polyphenolic compounds, and radical scavenging activity of rice cooked along with various mixed grains (barley, black soybean, adzuki beans, foxtail millet, proso millet, sorghum, glutinous rice) by following two different cooking methods (normal and pressure cooker). The amylogram and water characteristics of mixed grains showed significant differences based on the presence of different types of mixed grains. The chromaticity, palatability characteristics, presence of phenol compounds, and radical scavenging activity of rice cooked along with different mixed grains showed significant differences according to the nature of mixed grains. Total polyphenol contents of before cooking, cooked-rice added to mixed grains cooked in the normal cooker and a pressure cooker were 4.46~5.16, 0.58~0.93 and 0.65~0.96 mg GAE/g, and total flavonoid contents were 250.74~548.89, 129.26~207.04 and $127.41{\sim}218.15{\mu}g\;CE/g$, respectively. The DPPH radical scavenging activity of before cooking, cooked-rice added to mixed grains cooked in the normal cooker and a pressure cooker was 79.25~181.61, 22.07~53.64 and 7.51~39.97 mg TE/100 g, and ABTS radical scavenging activity was 203.25~328.24, 47.28~84.94 and 58.27~99.51 mg TE/100 g, respectively. Accordingly, it is necessary to different combinations of mixed grains according to the cooking method at home and grain industry.

In vitro Evaluation of Phalaris minor Seeds as Livestock Feed

  • Kaur, J.;Pannu, M.S.;Kaushal, S.;Wadhwa, M.;Bakshi, M.P.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.3
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    • pp.363-367
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    • 2006
  • The nutritional worth of Phalaris minor seeds was assessed in comparison to conventional cereal grains like maize and wheat. P. minor seeds had higher total ash and cell wall constituents as compared to wheat and maize grains. The CP content of P. minor was comparable to wheat grains but higher than maize grains. The in vitro studies revealed that the net gas production and availability of ME from P. minor was comparable to that of maize but the digestibility of nutrients was significantly (p<0.05) lower than that of conventional cereal grains. The digestion kinetic parameters for DM and CP revealed that P. minor had the highest (p<0.05) soluble fraction (a) followed by wheat and maize. Reverse trend was observed for insoluble but potentially degradable fraction (b). The effective and true DM and CP degradability was significantly (p<0.05) higher in wheat grains followed by that in P. minor and maize grains. The digestibility of OM and NDF was not affected by replacing cereal grains in concentrate mixture with P. minor seeds up to 75 per cent level. But the availability of ME from concentrate mixtures was comparable to control only up to 50% level of replacement. Replacement of cereal grains with P. minor did not affect the rapidly soluble fraction and insoluble but potentially degradable fraction of concentrate mixture containing P. minor up to 75 per cent, but it was depressed significantly at 100% replacement level. The effective and true degradability of DM of concentrate mixtures containing P. minor from 50 to 100 per cent was comparable to that of conventional concentrate mixture (CCM). The wheat based concentrate mixtures showed higher net gas production (208 vs. 201 ml/g DM/24 h), digestibility of nutrients and ME availability (9.64 vs. 9.54 MJ/kg DM) as compared to maize based concentrate mixture. The wheat based concentrate mixture had significantly (p<0.05) higher rumen undegradable fraction and effective degradability. The data conclusively revealed that conventional cereal grains could be replaced with P.minor seeds up to 75 per cent without affecting the availability of nutrients.