• The Plant Pathology Journal
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• v.34 no.2
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• pp.121-125
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• 2018

Maize (Zea mays L.; 2N=20) is major staple food crop grown worldwide adapted to several biotic and abiotic stresses. Maydis leaf blight (MLB) and banded leaf and sheath blight (BLSB) are serious foliar fungal diseases may cause up to 40% and 100% grain yield loss, respectively. The present studies were undertaken to work out the efficacy of chemicals, botanicals and bioagents for the management of MLB and BLSB under field condition for two seasons Kharif 2014 and 2015. Five molecules (propiconazole 25 EC, hexaconazole 25 EC, carbendazim 50 WP, mancozeb 75 WP and carbedazim 12 WP + mancozeb 63 WP), two bioagents i.e. Trichoderma harzianum and T. viridae and three botanicals namely azadirachtin, sarpagandha and bel pathar were tested for their efficacy against MLB. Eight newer fungicides viz., difenconazole 250 SC, hexaconazole 5 EC, carbendazim 50WP, validamycin 3 L, tebuconazole 250 EC, trifloxystrobin 50 WG + tebuconazole 50 WG, azoxystrobin 250 EC and pencycuron 250 SC were evaluated against BLSB. Analysis revealed significant effects of propiconazole at 0.1%, carbendazim 12 WP + mancozeb 63 WP at 0.125% and sarpagandha leaves at 10% against MLB pathogen, whereas validamycin at 0.1% and trifloxystrobin 25 WG + tebuconazole 50 WG at 0.05% were found effective against BLSB. The slow rate of disease control virtually by the bioagents might have not shown instant effect on plant response to the yield enhancing components. The identified sources of management can be used further in strengthening the plant protection in maize against MLB and BLSB.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.318-332
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• 2015

The maize production in South-eastern Asian countries showed a continuous increase with increasing poultry-livestock from the beginning of the 1990s to early 2010. Also the need for a new variety development of each contries was increased rapidly in the same period. Single-Cross hybrid varieties have been developed and supplied from 2001 instead of multi-cross maize varieties since 1992 in Indonesia. In Cambodia, CP group is mainly manufacturing feeds with most of the forage maize from farmers who are growing its seeds from the company. Cambodian main cultivars are varieties of multinational corporations such as DK8868 from Monsanto, NK6326, NK7328 from Syngenta and CP333 from CP group including local business company. Vietnam is the main maze importing country in South-Eastern Asia which had imported 13 times scale of amount compared to exports in average from 1990 to 2011. Vietnamese government has developed a range of varieties for improving their efficiency in production, such as the LVN-10 with political investments. Their production has been reached to 80% of the total. According to the 2012 MIFAFF (Ministry for Food, Agriculture, Forestry and Fisheries) data in Korea, domestic edible maize cultivation area was approximately 15,000ha. It showed 74,399 tons of production, 3.8% of food self-sufficiency in maize and around 0.9% of grain self-sufficiency rate. The consumption of grain is mostly rely on imports in Korea. To overcome the limit of the domestic seed market and increase maize self-sufficiency, the need to develop maze varieties for world-class is increasing at present through analyzing the market trend and prospect of the seed industry in South-eastern Asia.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.12
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• pp.1699-1707
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• 2000

Thirty-one multiparous Bos indicus${\times}$Bos taurus cows were offered concentrate supplements based on (1) 2 kg wheat bran; (2) 4 kg wheat bran; and (3) concentrate (30 maize: 67 wheat bran) at 0.5 kg per 1.0 kg milk produced, in a one year study in India. All supplements also contained 2 parts of a mineral mixture and 1 part salt. Cows were allocated to treatments at calving on parity (2nd and 3rd calf) with 13, 8 and 10 cows respectively in treatments 1, 2 and 3. They were individually fed for whole lactation, the basal diet being ad libitum berseem clover plus 2 kg wheat straw in the cool season/winter (period 1) and chopped maize in summer (period 2). Diets with berseem offered TDN and CP contents of 67.6, 18.2; 65.5, 16.8; and 67.5, 16.8 percent; and with maize fodder 62.6, 12.0; 62.6, 12.5; and 63.3, 12.5 percent for treatments 1, 2 and 3, respectively. Total dry matter (DM) intakes (1) 9.9 kg, (2) 10.9 kg and (3) 11.1 kg DM/day and intake of nutrients (TDN, CP) increased with level of supplementation (p<0.01), but effects of treatment on animal performance were not significant. Cow milk yields averaged (1) 7.9 kg, (2) 8.1 kg and (3) 8.8 kg milk/day (p>0.05) for lactation lengths of 252, 270 and 220 days (p>0.05) and cows gained +7.3; +8.1; and +12.0 kg respectively over their lactation (p>0.05). Wheat bran was used effectively as the sole energy component in concentrates for lactating dairy cows. Its use could potentially reduce feed costs and demands for cereal grain. Reduced concentrate levels may be considered if green forages of high nutrient content are fed ad libitum. Associated economic advantages or disadvantages require further evaluation.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1999.04a
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• pp.28.3-29
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• 1999

• Korean Journal of Plant Resources
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• v.17 no.3
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• pp.304-313
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• 2004

This study was conducted to find out the scientific maize breeding protocol for developing high performing single cross hybrid using introduced U.S. elite inbred lines; the pattern of inheritance, heterosis and heritabilities of six agronomic traits were studied in the progenies derived from five crosses (Mo17/B14A, Va85/B73, C103/ND203, FR35/Oh43, Wf9/A632). Among the five cross combinations, the cross combination of Mo17/B14A showed the highest heterosis for the most agronomic traits. Among 6 agronomic traits, the grain yield showed the highest heterosis effect in most cross combinations. Most of the agronomic traits in this study showed more than 50% heritability for six cross combinations, with an exception of the ear length trait. In conclusion, since Mo17/B14A showed excellent performance for most of the agronomic traits, these inbred lines were desirable combination and regarded as superior germ plasm sources for F1 hybrid development. The results of current studies will be utilized for developing high performing single cross hybrid from maize inbred lines, and will be used for the further genetic analysis of agronomic traits and maize breeding programs.

• The Plant Pathology Journal
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• v.34 no.2
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• pp.104-112
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• 2018

Accurate and rapid detection of bacterial plant pathogen is the first step toward disease management and prevention of pathogen spread. Bacterial plant pathogens Clavibacter michiganensis subsp. nebraskensis (Cmn), Pantoea stewartii subsp. stewartii (Pss), and Rathayibacter tritici (Rt) cause Goss's bacterial wilt and blight of maize, Stewart's wilt of maize and spike blight of wheat and barley, respectively. The bacterial diseases are not globally distributed and not present in Korea. This study adopted comparative genomics approach and aimed to develop specific primer pairs to detect these three bacterial pathogens. Genome comparison among target pathogens and their closely related bacterial species generated 15-20 candidate primer pairs per bacterial pathogen. The primer pairs were assessed by a conventional PCR for specificity against 33 species of Clavibacter, Pantoea, Rathayibacter, Pectobacterium, Curtobacterium. The investigation for specificity and sensitivity of the primer pairs allowed final selection of one or two primer pairs per bacterial pathogens. In our assay condition, a detection limit of Pss and Cmn was $2pg/{\mu}l$ of genomic DNA per PCR reaction, while the detection limit for Rt primers was higher. The selected primers could also detect bacterial cells up to $8.8{\times}10^3cfu$ to $7.84{\times}10^4cfu$ per gram of grain seeds artificially infected with corresponding bacterial pathogens. The primer pairs and PCR assay developed in this study provide an accurate and rapid detection method for three bacterial pathogens of grains, which can be used to investigate bacteria contamination in grain seeds and to ultimately prevent pathogen dissemination over countries.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1213-1220
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• 2018

Objective: An experiment was conducted to assess the antioxidant contents and activities of colored rice grains and to evaluate their nutritive characteristics in terms of chemical composition and in situ ruminal degradation. Methods: Ten cultivars of colored rice grains (Oryza sativa L.) collected from several areas of Japan were studied, and control rice without pigment, maize, barley, and wheat grains were used as control grains. Their chemical compositions, pigment, polyphenol contents, total antioxidant capacity (TAC), and degradation characteristics were determined. Results: The starch contents of the colored rice grains were in the range of 73.5% to 79.6%, similar to that of the control rice grain. The black and red rice grains contained anthocyanin (maximum: $5,045.6{\mu}g/g$) and proanthocyanidin (maximum: $3,060.6{\mu}g/g$) at high concentrations as their principal pigments, respectively. There were significantly (p<0.05) positive relationships among the pigment contents, polyphenol content, and TAC values in the colored and control rice grains, indicating that the increase in pigment contents also contributed to the increased polyphenol content and TAC values in the colored rice grains. The dry matter and starch degradation characteristics, as represented by c (fractional degradation rate of slowly degradable fraction) and by the effective degradability, of the colored rice grains and the control rice grain were ranked as follows among commonly used grains: wheat>barley${\geq}rice$>maize. The colored rice grains also included the most-digestible starch, since their potential degradable fraction and actual degradability at 48 h incubation were almost 100%. Conclusion: Colored rice grains have high potential to be used as antioxidant sources in addition to starch sources in ruminants.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.2
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• pp.188-194
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• 2002

To study the effect of wheat bran and lactic acid producing bacteria (LAB) on the performance of calves, 20 crossbred male cattle calves (day old), distributed into two groups were fed on calf starters containing 50 or 0% maize grain, along with green berseem ad libitum and milk as per body weight. Each group was further divided into two sub groups and one subgroup of each group was supplemented with mixed culture of LAB (Lactobacillus acidophilus L. casei, L. Jugarti). Milk feeding was discontinued after 8 weeks of age. The addition of culture increased (p<0.05) DM intake in calves receiving grainless diet from eighth week to the thirteenth one. There was about 21% higher body weight gain and 14% lower feed : gain ratio in culture supplemented calves. DM digestibility was significantly lower (p<0.05) in calves getting grain without culture. The crude protein NDF and ADF digestibility was higher (p<0.05) in grainless than the grain fed group. No major change on rumen fermentation pattern among different treatments was found. The concentration of total volatile fatty acids (TVFA) and protozoa count was higher (p<0.05) in grain fed group. However, lactic acid concentration was higher and rumen pH was lower due to culture feeding. The incidence as well as severity of diarrhoea was reduced in culture supplemented group. The results indicate that crossbred calves can be reared successfully on grainless diet and berseen fodder. The performance of calves was also improved by LAB supplementation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.4
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• pp.245-249
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• 1998

Soybean [Glycine max (L.) Merr.), mungbean [Vigna radiata (L.) Wilcz.], cowpea [V. unguiculata (L.) Walp.], adzuki bean [V. angularis (Willd.) Ohwi & Ohashi], maize [Zea mays L.], sorghum [Sorghum bicolor (L.) Moench], sorghum $\times$ sudangrass [So bicolor intraspecific hybrid], and Japanese millet [Echinochloa crusgalli var. frumentacea (Link) W.F. Wight] were grown at two planting dates (18 June and 15 July) at Cheju in 1997 to select the best forage legumes adapted to Cheju Island for grass-legume forage rotation. Averaged across planting dates and cultivars, dry matter (DM), crude protein (CP), and total digestible nutrient (TDN) yields were 5,646, 1,056, and 3,637 kg/ha for soybean, 4,458, 676, and 2,661 kg/ha for mungbean, 3,289, 553, and 2,055 kg/ha for cowpea, 3,931, 674, and 2,489 kg/ha for adzuki bean, 12,695, 969, and 7,642 kg/ha for maize, 17,071, 1,260, and 8,857 kg/ha for sorghum, 16,355, 1,163, and 8,543 kg/ha for sorghum $\times$ sudangrass hybrid, and 8,288, 929, and 4,091 kg/ha for Japanese millet. Soybean was higher in CP, ether extract (EE), and TON content but was lower in nitrogen free extract content compared with the three other legumes. The legumes had much higher CP (13.7 to 21.9%), EE (2.42 to 6.23%), and TDN (58.7 to 69.9%) content but lower in crude fiber (CF) content (17.3 to 25.3%) than did the grasses tested except maize which had relatively lower CF content but higher TDN content. These results suggest that soybean could be the best forage legume for grass-legume forage rotation in the Cheju region.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.11
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• pp.1650-1655
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• 2003

Crossbred gilts (n=54) of about $26.38{\pm}0.85kg$ body weight and 25 weeks of age were randomly divided into 18 groups of 3 animals each. Three dietary treatments viz., $T_1$, $T_2$ and $T_3$ were formulated. The treatment $T_1$ containing maize grain (35%) and wheat bran (47%) along with soybean meal and fish meals served as control diet. Rice bran (RB) was selected as a single source of fat (13.02%) and fibre (17.12%), which was gradually increased in diets $T_2$ (41%) and $T_3$ (82%) replacing maize grain and wheat bran of $T_1$ at 50% and 100% in the diets $T_2$ and $T_3$, respectively. The fat and fibre levels of the diets were thus 3.46 and 5.24, 9.31and 9.69 and 11.61and 13.26% in $T_1$, $T_2$ and $T_3$, respectively. All the diets, however, contained almost similar concentration of CP (18.35${\pm}0.29%$). Each dietary treatment was offered to six replicated groups of 3 piglets in following completely randomized design and feeding was continued for 112 days during the growing phase. Growth, feed utilization, reproductive performance, nutrient utilization and different blood biochemical parameters were studied. Growth rate, feed intake and feed conversion were lower (p<0.01) in $T_3$ in comparison to $T_1$ or $T_2$. Digestibility of all the nutrients except EE was reduced significantly (p<0.01) in $T_3$. Serum glucose level decreased (p<0.01), whereas the urea and cholesterol concentration in the blood increased (p<0.01) in $T_3$. The duration of estrus of pigs fed diet containing 82% RB ($T_3$) was highly variable within the group, but all the groups showed statistically similar duration of estrous. Feed cost per unit gain was found to be comparable between control ($T_1$) and $T_2$ group. The results indicated that RB can be included up to 41% in swine diets replacing 50% of maize and wheat bran. Higher concentrations of ether extract and fibre beyond 9.31 and 9.69% in diet had detrimental effect on growth, nutrient utilization and reproductive performance.