• Title/Summary/Keyword: grafting method

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Surface Modification of Fine Particle by Plasma Grafting in a Circulating Fluidized Bed Reactor under Reduced Pressure (감압 상태 순환유동층 반응기에서 플라즈마 그래프팅에 의한 미세입자 표면 개질)

  • Park, Sounghee
    • Korean Chemical Engineering Research
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    • v.53 no.5
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    • pp.614-619
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    • 2015
  • A plasma surface modification of powders has been carried out in a circulating fluidized bed reactor under reduced pressure. Polystyrene (PS) particles treated by plasma are grafted with polyethylene glycol (PEG) on the surface. The virgin, plasma-treated and grafted powders were characterized by DPPH method, FTIR, SEM and contact angle meter. The plasma-treated PS powders have well formed peroxide on the surface, By PEG grafting polymerization, PEG is well grafted and dispersed on the surface of the plasma-treated PS powders. The PEG-g-PS particle was successfully synthesized using the plasma circulating fluidized bed reactor under reduced pressure.

Efficient Transmission and Propagation of Tomato Chlorosis Virus by Simple Single-Leaflet Grafting

  • Lee, Huin;Kim, Mi-Kyeong;Choi, Hong-Soo;Kang, Jin-Ho;Ju, Ho-Jong;Seo, Jang-Kyun
    • The Plant Pathology Journal
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    • v.33 no.3
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    • pp.345-349
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    • 2017
  • Tomato chlorosis virus (ToCV), a member of the genus Crinivirus, has caused an epidemic disease in tomato worldwide. ToCV is phloem-limited and transmitted by whiteflies in a semi-persistent manner, but not by mechanical inoculation. Experimental propagation of ToCV has been performed primarily by using whitefly-mediated inoculation. To develop a simple and convenient method for transmission of ToCV, we investigated grafting single-leaflets from tomato plants infected with ToCV to recipient tomato seedlings. Forty-one of 46 tomato seedlings tested were grafted successfully with single-leaflets infected with ToCV. Among them, 36 seedlings (87.8%) were systemically infected with ToCV and developed typical symptoms. Our results demonstrated that single-leaflet grafting could provide a sufficient amount of inoculum for the transmission of ToCV to the grafted seedlings.

Migration and Distribution of Graft-inoculated Jujube Witches'-broom Phytoplasma within a Cantharanthus roseus Plant

  • Lee, Sang-Hun;Kim, Chul-Eung;Cha, Byeong-Jin
    • The Plant Pathology Journal
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    • v.28 no.2
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    • pp.191-196
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    • 2012
  • Periwinkle seedlings (Cantharanthus roseus) were inoculated with jujube witches'- broom (JWB) phytoplasma via grafting to analyze the migration of JWB phytoplasmas within the host plant. The phytoplasmas were detected using nested polymerase chain reaction (PCR) and fluorescence microscopy. Fluorescence microscopy was a simple and easy method of detecting phytoplasmas; however, it was not sufficiently sensitive to detect very low phytoplasma concentrations. Therefore, the migration of JWB phytoplasma was investigated through PCR. The first migration of JWB phytoplasma from an infected tissue to healthy tissues occurred late. After grafting, the phytoplasmas moved from the inoculated twig (or scion) to the main stem, which took 28 days. Afterward, the phytoplasma migrated faster and took less than 4 days to spread into the roots from the main stem. All twigs were then successively colonized by the JWB phytoplasmas from the bottom to the top. JWB phytoplasma was detected via nested PCR in all parts of the periwinkle seedling 82 days after inoculation. Based on these results, the inoculated JWB phytoplasma appeared to migrate downward to the roots along the main stem during the early stages, and then continued to move upward, colonizing twigs along the way until they reached the apex.

우위대마동맥을 이용한 관상동맥우회수술;임상적 및 혈관촬영에 의한 단기결과

  • 이현성;장병철;이성수;김재영;맹대현;박형동;윤영남;장양수
    • Journal of Chest Surgery
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    • v.33 no.2
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    • pp.151-159
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    • 2000
  • background: The right gastroepiploic artery(RGEA) has been use in coronary artery bypass grafting from 1987. The RGEA is the most useful arterial conduit in coronary artery bypass grafting(CABG) followed by the internal mammary artery, Materials and method: From Septermber 1998 to February 1999 the RGEA was used for coronary artery bypass grafting in 11 patients 10 males and 1 female. Postoperative angiography was performed in all of the patients before discharge Result: Early patent rate of the RGEA was 100%. The flow competition of the REGA graft was seen in 4 patients(36.4%) The flow pattern war RGEA dependent type in the inner diameter of the recipient coronary artery 1.5 mm the inner diameter of the RGEA 2.5 mm and the rtio of inner diameter of the RGEA and the recipient coronary artery 1(p<0.05) Conclusion : Early results of CABG with RGEA was satisfactory. However the RGEA graft has a tendency of flow competition in relation to the inner diameter of graft. Preoperative angiographic evaluation for RGEA and meticulous operative technique are required for a good surgical results.

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Histochemical and Cytochemical Studies on the Secondary Xylem Formation during Grafting Process of Pinus thunbergii (접목 과정에서 형성된 신생 2차목부의 조직학적, 세포화학적 연구)

  • Kim, Jong Sik;Lee, Kwang Ho;Kim, Yoon Soo
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.6
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    • pp.1-11
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    • 2006
  • Grafted tissues were investigated using various microscopic techniques. Pinus thunbergii was used as stock and scion and autografted by cleft graft method. Histochemically, grafting processes can be proceeded by four stages: 1) formation of necrotic layer, 2) proliferation of callus, 3) development of neo-cambium from callus, and 4) restoration of new vascular xylem. Necrotic la yer composed of pectin and lignin was gradually degraded during grafting process and disappeared when new union was formed between stock and scion. A large number of starch and lipid bodies in the cytoplasm were also gradually degraded during grafting process and disappeared at the grafting interface. Nucleus and plasmodesmata were not changed. Bubble-like callus was generated from all living parenchyma cells and from the callus. The tracheary elements differentiated from the callus had either reticulate or pit-like thickenings in the secondary walls with bordered pits. Secondary cell wall thickening occurred toward filing to the void parts between reticulated secondary wall. Tracheids formed in the secondary xylem were short with irregular wall thickness. New secondary xylem cells with swirled shapes, which developed in graft union were oriented horizontally and obliquely to axis of the stem.

Radiation Grafting of Hydrophilic Monomers onto Polyester

  • Park, Jae-Ho;Lee, Chong-Kwang;Lee, Kwang-Jin
    • Nuclear Engineering and Technology
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    • v.5 no.2
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    • pp.103-114
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    • 1973
  • Radiation grafting of acrylic acid and 4-vinylpyridine at room temperature has been studied by an impregnation method to improve the hygroscopic properties, the antistatic behavior and the dyeability of polyester fabric. Polyester fabric was impregnated with acrylic acid or aqueous emulsion of acrylic acid-4-vinylpyridine by immersion at 25$^{\circ}$or 7$0^{\circ}C$. The impregnated fabric was irradiated under nitrogen gas with ${\gamma}$-rays from Co-60. When acrylic acid grafted polyester fabric was treated with sodium carbonate, calcium acetate and potassium persulfate, tne rate of water absorption was increased and most parts of polyacrylic acid formed were extracted off from the fabric with 0.1% solution of sodium hydroxide at 10$0^{\circ}C$. In the case of the impregnation of a mixture of acrylic acid and 4-vinylpyridine the petcent of grafting has been shown to be proportional to the ratio of 4-VP/AA and radiation dost. Estimating by contact angle measurements of water on the various polymer surfaces, the antistatic behavior was decreased with the increase of grafting percent. The investigation of electron micrograph disclosed the existence of certain type of discontinuities in the acrylic acid grafted polyester fiber which was treated with various salts.

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Coronary Artery Bypass Grafting without Cardiopulmonary Bypass -one case report- (임공심폐기를 사용하지않는 관상동맥우회술 -1례 보고-)

  • Na, Chan-Young;Lee, Young-Tak;Kim, Woong-Han;Chung, Chul-Hyun;Jung, Yoon-Seop;Bang, Jeong-Hyin;Kim, Wook-Seong;Lee, Sub;Han, Jae-Jin;Chung, Do-Hyun;Chung, Ill-Sang;Park, Jung-Won;Park, Young-Kwan;Hong, Sung-Nok;Moon, Hyun-Soo
    • Journal of Chest Surgery
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    • v.29 no.11
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    • pp.1267-1269
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    • 1996
  • Coronary artery bypass grafting without cardiopulmonary bypass is now one of accepted technique of myocardial revascularization. This technique is an alternative method for patients with lesions in the left anterior descending coronary artery and right coronary artery. We report a case of coronary artery bypass grafting without cardiopulmonary bypass or cardiac arrest.

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Evaluation of the Effect of Solvent on the Preparation of PVBC-g-ETFE Film by a Pre-irradiation Method (전조사법에 의한 PVBC-g-ETFE 필름 제조 시 용매의 영향 평가)

  • Lee, Sun-Young;Song, Ju-Myung;Sohn, Joon-Yong;Nho, Young-Chang;Shin, Jun-Hwa
    • Polymer(Korea)
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    • v.35 no.6
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    • pp.610-614
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    • 2011
  • In this study, the effect of solvent on the pre-irradiation grafting of VBC(vinylbenzyl chloride) onto a ETFE(polyethylene-co-tetrafluoroethylene) was evaluated. ETFE film was irradiated to generate radical species onto its backbone chain. Each irradiated film was immersed into VBC monomer mixtures diluted with various solvents such as toluene, heptane, and isopropanol etc. for grafting process and then the degree of grafting of each film was measured. FTIR analysis confirmed that the VBC-g-ETFE film was successful prepared. For the films prepared in the various solvents, the mechanical strength and the distribution pattern of the graft polymer over the cross-section of the films were measured and the effect of solvent was evaluated.

Comparison of minimally invasive versus conventional open harvesting technique for iliac bone graft in secondary alveolar bone grafting in cleft palate patients: a systematic review

  • Saha, Aditi;Shah, Sonal;Waknis, Pushkar;Bhujbal, Prathamesh;Aher, Sharvika;Vaswani, Vibha
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.45 no.5
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    • pp.241-253
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    • 2019
  • This study evaluated and compared the donor site morbidity following minimally invasive and conventional open harvesting of iliac bone for secondary alveolar bone grafting in cleft palate patients. A thorough electronic search of PubMed, Google Scholar, EMBASE, and an institutional library and manual search of various journals was done; Inclusion criteria: 1) full-text articles using a minimally invasive or conventional open harvesting technique for iliac bone for secondary alveolar grafting in cleft palate patients and 2) articles published between January 1, 2001 and June 30, 2017 and Exclusion criteria: 1) articles published in languages other than English, 2) case reports, case series, animal studies, in vitro studies, and letters to the editor, and 3) full-text article unavailable even after writing to the authors. Preliminary screening of 274 studies excluded 223 studies for not meeting the eligibility criteria. Of the remaining 51 studies, 19 were removed for being duplicates. Of the remaining 32 studies, 15 were excluded after reading the abstract. Of the 17 studies that were left, 2 were excluded because they were in a language other than English, and 2 were excluded because the study group did not mention cleft palate patients. Thus, 13 studies providing results for a total of 654 patients were included in this qualitative synthesis. Minimally invasive bone graft harvest techniques are better than the conventional open iliac bone harvest method because they offer shorter operative time, decreased requirement for pain medications, less pain on discharge, and a shorter hospital stay.