• 한국응용과학기술학회지
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• 제26권3호
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• pp.248-262
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• 2009

This study is on the feasibility of use of glycoprotein in various areas such as cosmetics and food etc. by extracting, isolating and refining glycoprotein from carrots, red ginseng extract residue, sesame and pine needles using protease(pepsin) and by analyzing general characteristics and measuring various bioactivities. The results of analysis of nutritional composition showed protein contents of glycoprotein. In the analysis of constitutive amino acids, the ratio of contents of hydroxy proline and glycine, the characteristics of glycoproteins appeared similar and the contents of glutamic acid and aspartic acid appeared higher. As a result of measurement contents of total polyphenol and flavonoid, it showed that glycoprotein had more contents generally, and the effect of bioactivity of glycoprotein appeared higher although different kinds of glycoprotein showed a little DPPH radical and nitrite scavenging ability, total antioxidant capacity by ABTS, ACE inhibitory.

• 한국환경과학회지
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• 제31권1호
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• pp.23-31
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• 2022

As a herbal supplement, Dioscorea batatas Decne (DBD) presents potent antioxidant activity and diverse health benefits. In the present study, functions of a 30 kDa glycoprotein isolated from DBD (hereafter, DBD glycoprotein) in the regulation of feed efficiency and fecal malodor in mice were explored. DBD glycoprotein produced protective effect against cytotoxicity induced by the ecotoxicological endocrine-disrupting substance bisphenol A in gastrointestinal epithelial HT-29 cells. To investigate its potential roles in the regulation of feed efficiency and fecal malodor, mice were administered an oral injection of DBD glycoprotein for 2 weeks. Compared with the control values, the weight of internal organs (liver, heart, kidney, and spleen) and levels of glutamate pyruvate transaminase, glutamate oxaloacetate transaminase, and lactic dehydrogenase were not significantly changed during DBD glycoprotein administration for 2 weeks. Interestingly, DBD glycoprotein improved feed efficiency and reduced hydrogen sulfide concentration without altering the ammonia level in mouse feces. Collectively, these results indicate that DBD glycoprotein is a functional agent that exerts gastrointestinal protective effects against ecotoxicological substances, improves feed efficiency, and reduces fecal malodor.

• 한국환경과학회지
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• 제29권3호
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• pp.241-247
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• 2020

Ulmus davidiana Nakai (UDN) has been traditionally used as a herbal medicine in Korea. In the present study, we investigated the anti-ecotoxic potential of a 116 kDa glycoprotein isolated from UDN (UDN glycoprot ein) in regulating fecal malodor and feed efficiency in mice. We found that UDN glycoprotein (200 μg/ml) has an inhibitory effect on the cell death induced by an ecotoxicological endocrine disrupting chemical, bisphenol A, in colon epithelial HT-29 cells. UDN glycoprotein did not show significant differences regarding the weight of ecotoxicity-related organs (liver, heart, kidneys, and spleen) and the levels of serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, and lactate dehydrogenase in mice for 2 weeks, compared to the control. Additionally, UDN glycoprotein reduced the levels of hydrogen sulfide and ammonia as markers of fecal malodor in mice. Interestingly, UDN glycoprotein can improve the mouse feed efficiency. In conclusion, our data indicate that anti-ecotoxicological UDN glycoprotein has the ability to increase the feed efficiency and reduce the fecal malodor by maintaining the viability of colonic epithelial cells in mice.

• 한국환경과학회지
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• 제28권2호
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• pp.249-257
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• 2019

Ulmus davidiana Nakai (UDN) has been traditionally used as a herbal medicine to treat inflammatory diseases in Korea. In the present study, we investigated the anti-ecotoxic potential of a 116 kDa glycoprotein isolated from UDN (UDN glycoprotein) in human intestinal epithelial INT-407 cells. We demonstrated that UDN glycoprotein ($20{\mu}g/mL$) could inhibit the production of lactate dehydrogenase (LDH) induced by toluene, an ecotoxic substance. Additionally, we found that the toluene-induced intestinal cytotoxicity was mediated by the phosphorylation of p38 Mitogen-Activated Protein Kinase (MAPK) via the production of intracellular Reactive Oxygen Species (ROS). The UDN glycoprotein significantly decreased the levels of ROS production and p38 MAPK activation in toluene-stimulated INT-407 cells. Moreover, the UDN glycoprotein inhibits the phosphorylation of nuclear factor-kappa B ($NF-{\kappa}B$), which is responsible for the production of LDH, in toluene-stimulated INT-407 cells. Collectively, our data indicate that UDN glycoprotein is a natural antioxidant and a modulator of ecotoxicity signaling pathways in human intestinal epithelial cells.

• 한국동물학회지
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• 제33권2호
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• pp.191-199
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• 1990

토끼의 골격근에서 근소포체를 순수 분리하여 SDS-polyacrylamide gel전기영동법과 125 I-concanavalin A표지법으로 단백질과 당단백질의 조성을 조사하였다. 전기영동사에 나타난 대표적인 단백질은 $Ca^2$+-AThase, 80 Kd protein,calsequestrin,high affinity calcium binding protein, intrinsic glycoprotein이었으며, 160 Kd protein, 94 Kd protein,38 Kd protein, 34 Kd protein,24 Kd proteins도 존재하였다.특히, 막성계에 있는 heak protein으로 알려져 있는 80 Kd protein은 본 연구를 통해 주로 근소포체의 terminal cisternae에 들어 있음이 확인되었다. 한편 125 I-concanavalin A표지에 의해 전기영동성에 나타난 대표적인 당단백질은 160 Kd glycoprotein, 94 Kd glycoprotein, calsequestrin, intrinsic glycoprotein의 4종이었다. 이 가운데 94 Kd glycoprotein은 94 Kd glucose-regulated protein으로 추정되며, 본 연구를 통해 근소포체에서도 특히 T-tubule에 다량으로 존재함이 밝혀졌다.

• 한국어병학회지
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• 제30권2호
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• pp.71-78
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• 2017

The glycoprotein of novirhabdoviruses is known to play a critical role in the determination of host specificity. Viral hemorrhagic septicemia viruses (VHSVs) in different genotypes have different glycoprotein sequences and show different preferences for specific cell lines. In this study, to know whether the glycoprotein is solely responsible for the host cell preference of VHSV, a recombinant VHSV expressing vesicular stomatitis virus (VSV) glycoprotein instead of VHSV IVa glycoprotein (rVHSV-VSV-G) was generated by reverse genetics and inoculated into several fish cell lines, then, cytopathic effect (CPE) and viral growth caused by rVHSV-VSV-G infection were compared with those caused by rVHSV-wild that was previously generated and has the same genomic sequence with wild-type VHSV except a few nucleotides. The plaque numbers of rVHSV-VSV-G were significantly higher in EPC, BF-2 and GF cells than those of rVHSV-wild. However, in HINAE cells (originated from olive flounder), rVHSV-VSV-G titer was significantly lower than rVHSV-wild titer, and both recombinant VHSVs were not grown well in CHSE-214 cells. Although statistical significances were detected in the titers between rVHSV-wild and rVHSV-VSV-G in several cell lines, the cell line-preference order of rVHSV-VSV-G was not different from that of rVHSV-wild. These results suggest that the replacement of VHSV glycoprotein may not completely change host cell preference, and other regions of VHSV might also involve in the determination of host cell preference.

• International Journal of Industrial Entomology and Biomaterials
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• 제29권2호
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• pp.207-213
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• 2014

The structural proteins of classical swine fever virus (CSFV) consist of nucleocapsid protein C and envelope glycoprotein $E^{rns}$ (E0), E1 and E2. Among them, E2, the most immunogenic of the CSFV glycoproteins, induces a protective immune response in swine. In this study, to determine the optimal expression conditions of glycoprotein E2 using baculovirus system, we investigated the influence of insect cells and media to the expression of recombinant E2. Recombinant virus containing glycoprotein E2 coding gene was constructed with bApGOZA DNA. Expression of the glycoprotein E2 was analyzed by SDS-PAGE and Western blot analysis using anti-CSFV E2 monoclonal antibodies. Expression of glycoprotein E2 in Sf21 cells was first observed after 3 days and reached a maximum on the 5th day after infection. Furthermore, the highest levels of glycoprotein E2 expression were observed at multiplicity of infection (MOI) of 5. When three different insect cell lines (Sf21, High-Five and Se301) were tested, High-Five cells showed the highest production. In addition, four different serum-free and serum-supplemented media, respectively, were tested for the expression of glycoprotein E2 and the budded virus (BV) titers. As a result, serum-supplemented medium provided the best conditions for protein production and the BV yield.

• 공업화학
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• 제24권1호
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• pp.62-66
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• 2013

본 연구는 당근에서 추출한 당근당단백질(carrot glycoprotein, 이하 CG라 명함)의 물리화학적인 특성을 조사하기위하여 수행되었다. 천연 식물성 원료인 당근에서 CG를 제조하여 물리화학적인 특성을 분석하였다. 영양성분 조성을 분석한 결과 CG는 당단백질로서 2.35%의 탄수화물과 94.2%의 단백질로 구성되어있는 것으로 나타났다. 아미노산 조성분석 결과 CG는 콜라겐 펩타이드의 특징인 hydroxyproline과 glycine은 소량 검출되었으며, 포도당과 지방 대사에 관여하는 glutamicacid와 asparticacid가 높게 검출되었다. 또한 열량 분석결과 100 g의 CG는 342.1 kcal의 열량을 지니고 있는 것으로 나타났다. CG의 분자량 분석 결과에서는 594 Da 이하의 평균분자량 분포를 나타내는 특성을 가지고 있는 것을 알 수 있었다.

• 대한치과의사협회지
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• 제10권9호
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• pp.585-586
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• 1972

The demonstration of glycoprotein in developing mandibular bone was observed histochemically, using Clara method and Von Kossa method. The glycoprotein reaction showed slight activity in New bilding bone matrix.

• 한국식품과학회지
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• 제37권4호
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• pp.624-630
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• 2005

본 연구는 천연물에서의 생리활성물질 동정의 일환으로 Ficus Carica Linnoeus(FCL)로부터 60kDa의 FCL glycoprotein(무화과 당단백질)을 추출한 후, 무화과 당단백질의 첨가에 따른 과산화 지질 라디칼 억제능력 및 생질의 혈장 콜레스테롤 수준과 간 해독효소활성의 개선효과를 평가하였다. In vitro에서 리놀렌산 자동산화반응에 기초한 과산화 지질 라디칼 억제능력을 실험한 결과, 무화과 당단백질을 섭취시킨 농도가 증가함에 따라 과산화 지질 라디칼 억제율은 증가하였다. 한편, 생쥐에게 14일 동안 무화과 당단백질을 50 및 100mg/kg 농도로 섭취시킨 그룹과 무화과 당단백질을 섭취시킨 후 Triton WR-1339를 투여한 생쥐 그룹에서 혈액 및 간조직을 채취하여 혈장 콜레스테롤의 수준변화 및 해독효소의 활성을 측정한 결과, 100mg/kg 농도로 무화과 단백질을 섭취시킨 그룹에서 TC와 LDL-콜레스테롤의 수준은 유의적 감소효과가 나타났다(p<0.05). 또한 Triton WR-1339에 의해 고지혈증이 유발된 생쥐그룹에서도 TC와 LDL-콜레스테롤 수준이 유의적 억제능력을 보였는데, 특히 100mg/kg 농도에서 그 개선 효과는 더욱 분명하였다(p<0.01). 간의 해독효소 중 항산화 기능을 하는 SOD, CAT 그리고 GPx의 활성은 모두 증가되었는데, 특히 GPx는 100mg/kg의 농도에서 유의성을 보이며 증가하였다(p<0.01). 따라서 이러한 결과를 종합하면, 무화과 당단백질이 해득효소의 활성을 증가시킴으로써 체내의 ROS의 수준을 감소시키고, 이러한 항산화 효과가 혈중 콜레스테롤의 수준을 감소시키는데 영향을 미친 것으로 사료된다.