• Applied Microscopy
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• 제19권2호
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• pp.99-118
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• 1989

The cardiotoxic effects of acute and chronic administration of adriamycin (ADR) were evaluated in A/J Swiss albino mice. In acute studies, male mice received intravenous ADR, 5mg or 15mg/kg per day for 3 or 1day and were sacrifice 12 hours later. Because the glutathione-glutathione peroxidase system is major pathway for free radical detoxication, glutathione levels and glutathione peroxidase activity was measured. In acute studies, ADR-treated mice exhibited significantly decreased levels(p<0.05) of total glutathione and unchanged levels of oxidized glutathione and percentage of oxidized glutathione. The earliest myocardial fine structural alterations included swelling and degeneration of mitochondria and dilatation of sarcoplasmic reticulum at all dosage of acute models. In chronic studies, mice received 5mg/kg ADR once a week for up to 16 weeks. Levels of total and reduced glutathione were decreased significantly(p<0.01) and oxidized glutathione and percentage of oxidized glutathione were increased significantly (p<0.05). Chronic myocardial lesions included perinuclear vacuolization, seperation of myofibrils and the fasciae adherens of intercalated disc and hypercontraction band within myocyte. Glutathione peroxidase activity reduced significantly (p<0.01) in any group of acute and chronic ADR-treated animals. Test for lipid peroxidation(malondialdehyde) was increased significantly(P<0.01). Thus, we conclude 1) ADR significantly lowers glutathione levels in heart tissue, and 2) cellular damage progress produced by alteration of this system in mouse models of ADR cardiotoxicity. These results suggest that the glutathione-glutathione peroxidase system may be involved in the modulation of ADR-induced cardiotoxicity.

• 약학회지
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• 제44권3호
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• pp.237-244
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• 2000

Liver isolated from 18 hours fasted rats was subjected to $N_2$hypoxia (for 45 min) followed by reoxygenation (for 30 min). The perfusion medium used was Krebs-Henseleit bicarbonate buffer (pH 7.4, $37^{\circ}C$). Vitamin C (0.5 mM) and trolox C (0.5 mM), soluble vitamin E analog, were added to perfusate. Lactate dehydrogenase (LDH), total glutathione, oxidized glutathione, lipid peroxide and drug-metabolizing enzymes were measured. After hypoxia LDH significantly increased but this increase was attenuated by vitamin C and combination of vitamin C and E. Total glutathione and oxidized glutathione in perfusate markedly increased during hypoxia and this increase was inhibited by vitamins C, E and its combination. Similarly; oxidized glutathione and lipid peroxide in liver tissue increased after hypoxia and reoxygenation and this increase was inhibited by vitamin I and combination of vitamin C and E. Hepatic drug metabolizing function (phase I, II) were suppressed during hypoxia but improved during reoxygenation. While vitamins C and E only increased glucuronidation, the combination of vitamin C and E increased the oxidation, glucuronidation and sulfation. Our findings suggest that vitamins C and E synergistically ameliorates hepatocellular damage as indicated by abnormalities in drug metabolizing function during hypoxia/reoxygenation and that this protection is in major part, caused by decreased oxidative stress.

• Journal of Ginseng Research
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• 제24권3호
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• pp.113-117
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• 2000

적변인삼의 원인 물질을 구명코저 인삼뿌리의 적변현상과 항산화 활성과의 상관관계를 조사하기 위하여 적변뿌리와 건전뿌리의 각 부위별 항산화 활성을 조사하였다. 적변삼의 표피와 피층조직의 superoxide dismutase(SOD) 활성(units/mg protein)은 각각 126.9와 79.6으로 정상뿌리에 비해 각각 3.5배, 2.0배 높았다. 적변뿌리의 catalase활성은 약 1.6배 높았으나, peroxidase 활성은 오히려 약간 낮았다. 적변뿌리 각 조직의 DPPH 자유라디칼 포착활성은 정상뿌리에 비해 약 2.0배 높았다. 정상뿌리의 ascorbate 함량은 조직에 따라 약간의 차이를 나타내었지만(166~240 $\mu$g/g fr. wt.), 산화형dehydroascorbate(DHA) 함량이 전체 ascorbate 함량의 약 97~79%를 차지하였다. 적변뿌리의 전체 ascorbate함량은 정상뿌리와 비슷하였지만 환원형 ascorbate의 함량은 조직에 따라 다르지만 정상뿌리에 비해 3.5~7.5배 높았다. 적변뿌리의 표피조직, 피층조직, 중심주 조직의 전체 glutathione 함량은 정상조직에 비해 각각 7.3배, 4.8배, 1.2배 높았다. 적변화에 따른 환원형 glutathione(GSH)과 산화형 glutathione(GSSG)의 함량비는 전체 glutathione 함량변화와 비슷하였다. 이상의 결과는 인삼의 적변조직은 어떤 외부스트레스에 의해 과다하게 발생되는 활성산소종을 제거하기 위하여 SOD 활성, 환원형 ascorbate 함량, 환원형 glutathione 함량이 크게 증가하는 것으로 사료된다.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2002년도 제9회 학술 발표회 프로그램과 논문초록
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• pp.48-48
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• 2002

Glutathione (GSH) is most prevalent reducing thiols in eukaryotic cells and known that participates in many cellular processes. It was found that total amount of glutathione and the ratio of reduced to oxidized glutathione during development of Dictyostelium discoideum increase at the initial stage of the aggregation of amoeba.(omitted)

• Journal of Nutrition and Health
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• 제33권5호
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• pp.502-506
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• 2000

Effects of powdered siho(bupleuri radix) on serum and liver lipid composition and antioxidative capacity were investigated in rat fed high oxidized fat. Sixty male Sprague-Dawley rats weighing 161.25$\pm$2.51g were blocked into four groups according to body weight and raised seven weeks with basal diet(normal group), basal diet and 10% oxidized fat(control group), basal diet, 10% oxidized fat and 2% powdered siho(2% powdered siho group) and basal diet, 10% oxidized fat and 3% powdered siho(3% powdered siho group). Feed intake, body weight gain and feed efficiency were not significantly different among oxidized fat diet groups. The values of total cholesterol and triglyceride in plasma and liver showed no significantly different(p>0.05) in the oxidized fat diet groups. Plasma HDL-cholesterol showed atendency to increase in powdered siho diet groups. The values of thiobarbituric acid in plasma and liver were lower in the powdered siho diet groups than control group. Plasma GOT and GPT activity showed a tendence to decrease in powdered siho groups. Liver glutathione peroxidase activity showed a tendence to increase in powdered siho diet groups. Thus, it seems that powdered siho had some antioxidative elements.

• 한국식품영양과학회지
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• 제20권4호
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• pp.285-292
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• 1991

에탄올의 급성적 및 만성적 투여가 흰쥐의 간 및 소뇌 중의 glutathione(GSH) 양상과 지질과산화물 수준에 미치는 영향을 알아 본 결과는 다음과 같다. 간조직에서는, 만성적 에탄을 투여 (6~9g/kg, per day, 10% 음료수로서, 4주간)에 의해 총 GSH 농도가 14. 5% 저하되었고, 산화형 GSH(GSSG)는 변화가 없었으며, 따라서 GSSG/총 GSH 비율은 증가하였다. 그러나 지질과산화 수준은 변함이 없었다. 급성적 에탄올 투여에 의해 간 조직 중 지질과산화 수준이 상승되고 총 GSH 농도가 감소함은 이미 보고되고 있다. 본 실험에서는 이와 관련시켜 급성적 에탄을 투여 (50mmole/kg, i.p.)후 post-hepatic 혈장 중의 총 GSH 수준을 측정한 결과 현저히 상승하였다. 이러한 GSH의 간에서 혈액으로의 유출은, GSH의 항산화적 소모 이외에도, 급성적 그리고 아마도 만성적인 에탄을 투여에 의한 간 조직 중의 총 GSH의 감소의 한 가능한 원인으로서 간접적으로나마 추정될 수 있겠다. 소뇌에서 는 급성적 에탄을 투여는 지질과산화 수준을 증가시켰으나 GSH 양상은 변화시키지 않았으면, 만성적인 경우엔 모두 변동시키지 못하였다.

• Archives of Pharmacal Research
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• 제20권5호
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• pp.471-475
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• 1997

Livers isolated from 18 hours fasted rats were subjected to N$_{2}$ hypoxia (for 45 min) followed by reoxygenation (for 45 min). The perfusion medium used was Krebs-Henseleit bicarbonate buffer (KHBB, pH 7.4). Lactate and alanine were added as gluconeogenic and ureagenic substrates and Trolox C was also added to perfusate. Oxygen consumption, lactate dehydrogenase (LDH), alanine transaminase (ALT), total glutathione, oxidized glutathione, bile flow, glucose and urea were measured. After hypoxia oxygen consumption significantly dropped but Trolox C had no influence on this decrease. ALT and LDH were significantly increased by hypoxia/reoxygenation. This increase was markedly attenuated in the presence of Trolox C. The total glutathione and oxidized glutathione efflux increased following hypoxia, which were prevented by the treatment of Trolox C. Bile flow rate decreased following hypoxia/reoxygenation but did not continue to decrease in the reoxygenation phase by Trolox C. Following hypoxia/reoxygenation glucose and urea releases decreased. Trolox C had no influence on inhibition of glucose and urea production. These results suggest that Trolox C protected the liver cells against hypoxia/reoxygenation injury, yielding further evidence for a causative role of oxidative stress in this model.

• 대한약침학회지
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• 제7권3호
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• pp.37-46
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• 2004

Effects of Boonsimgieum aqua-acupuncture at $Naegweun(HP_6)$ and $Taecheung(LV_3)$ on liver and plasma lipid composition and antioxidative capacity were investigated in rat fed high oxidized fat. Concentrations of plasma triglyceride, total cholesterol and LDL-cholesterol showed a tendency to increase in the high oxidized fat diet group. However these values showed a tendency to decease in aqua-acupuncture groups. HDL-cholesterol showed no significantly different in all the treatment groups. Liver total cholesterol values showed a high in control group, however other treatment groups showed no significantly different. Liver triglyceride concentration showed a high value in control group and $Naegweun(HP_6)$ aqua-acupuncture group showed lower value than $Taecheung(LV_3)$ aqua-acupuncture group. Plasma GOT and GPT values showed a tendency to increase in high oxidized fat diet group. However aqua-acupuncture groups showed a lower values than control group. The concentration of TBARS in liver and plasma showed a high values in high oxidized fat diet group, however these values showed a tendency to decrease in aqua-acupuncture group. Glutathione peroxidase, superoxide dismutase and catalase activity values showed a low values in high oxidized fat diet group, however these values showed a tendency to increase in aqua-acupuncture groups and in aqua-acupuncture groups, these values showed no significantly different.

• 대한본초학회지
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• 제25권1호
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• pp.13-21
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• 2010

Objectives : This study was purposed to the anti-oxidative effects of Polygonati Multiflori Caulis(henceforth PMC) on oxidized brain and liver cells in rats. Methods : After extraction of PMC with water, the water extract was divided into five fractions : hexane, ethyl ether, ethyl acetate, butanol and an aqueous fraction. The phenol contents of each fraction were measured. The lipid peroxidation inhibition effect were then investigated. Results : After processing PCM water and PCM fractionations on oxidized brain cells in rats, the SOD (super oxide dismutase) activity and glutathione content were increased, and the NO (nitric oxide) content was decreased. It had much higher SOD activity than liver cells in rats excluded in the n-BuOH and aqueous fractions. In case of oxidized liver cells in rats, the SOD activity and glutathione content increased, while both the NO content and the MDA (malondialdehyde) content decreased. It had much higher glutathione content than brain cells in rats in the every fractions. It had much lower MDA content than brain cells in rats in the Aqueous fractions and brain cells in rats had much lower MDA content than liver cells in rats in the total extract, n-hexane, EtOEt, EtOAc and n-BuOH fractions. Conclusions : PMC has anti-oxidative effect on oxidized liver cells and brain cells in rats, through there are differences in fraction. Additionally, Anti-oxidative effect of brain cells can be relaxed the mental nerve and it is related PMC effect.

• 한국자원식물학회지
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• 제17권3호
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• pp.323-330
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• 2004

금앵자 추출물이 과산화지 질을 급여한 횐 쥐의 혈장 및 간장지질구성과 항산화능에 미치는 영향을 검토했다 혈장 총 콜레스테롤 량은 과산화지질 첨가군 모두가 정상군보다 증가하였으나, 금앵자 추출물 첨가에 의해 하락하였다. 혈장HDL-cholesterol량은 금앵자 첨가군이 대조군 보다 높은 경향을 나타내었다. 혈장 중성지질량은 정상군과 금앵자 첨가군은 유의한 차이를 나타내지 않았으며, 대조군 보다 낮은 수치를 보였다. 간장의 총 콜레스테롤 량은 전 처리군에서 유의한 차이를 나타내지 않았으며, 중성지질 량은 정상군과 금앵자 첨가군은 유의한 차이를 나타내지 않았으며, 대조군 보다 낮은 수치를 보였다 혈장내 TBARS량은 정상군과 금앵자군은 유의한 차이를 나타내지 않았으며, 대조군 보다 낮은 수치를 보였다. 간장내 TBARS량은 과산화지질 첨가에 의해 증가하였으나, 금앵자 첨가에 의해 하락했다. 혈장 GOT 및 GPT의 활성치는 관산화지질 첨가군에서 증가하였으나, 금앵자처리에 의해 하락했다(P＆lt;0.05). 간장 GSH-Px, SOD 및 CAT활성치는 금앵자처리에 의해 증가하는 경향을 보였다.