• 한국미생물·생명공학회지
• /
• 제2권2호
• /
• pp.103-109
• /
• 1974

전국 각지대의 279개소의 토양시료로 부터 초산자화성세균 383균주를 분리하였으며 이들 초산자화성세균중 L-GA생성 능이 우수한 5균주를 선정하고 균학적성질을 조사하여 Brebacterium flavum nov. sp. D1005B, Corynebacterium glutamicum nov. sp. D1025A, Brevibacterium. flavum nov. sp. D2209B, Corynebacterium acetoacidophilum nov. sp. D2212B 및 Corynebacterium acetoacidophilum nov. sp. D2349A로 동정 명명하였다

• Journal of Microbiology and Biotechnology
• /
• 제25권4호
• /
• pp.554-558
• /
• 2015

Drug delivery systems (DDSs) incorporating bacterial minicells have been evaluated as a very powerful tool in view of biocompatibility. However, limited studies have been carried out on these systems, mainly using minicells from Salmonella sp. and Escherichia coli. Thus, we generated a new minicell-producing strain from an endotoxin-free Corynebacterium glutamicum by the inactivation of genes related to cell division. The two knockout strains, ${\Delta}parA$ and ${\Delta}ncgl1366$, showed distinct abilities to produce minicells. The resulting minicells were purified via sequential antibiotic treatments and centrifugations, which resulted in reproducible yields.

• 미생물학회지
• /
• 제22권4호
• /
• pp.249-255
• /
• 1984

To develop the host-vector system for industrial Coryneform bacteria that seemed to be the most suitable microorganisms for molecular breeding of genes involved in the production of amion acids, nucleotides, and other products of industrial interest, broad host range E. coli plasmid R 1162 DNA was transformed into Brevibacterium ammoniagenes and the plasmids pKU6 isolated from a transformant was physically characterized. All other plasmids from the transformed cells except pKU6 exsisted as multimeric forms in Brevibacterium ammoniagenes. The plasmid DNA was retransformed into Corynebacterium glutamicum with a high frequency ($1.32{\times}10^{-1}$ per cell) and maintained stably both in Brevibacterium ammoniagenes and Corynebacterium glutamicum after 100 generations of cultures with 25-30 copy number per cell. The size of both plasmid pKU6 and plasmid R1162 were the same and restriction maps by EcoR I, Ava I, Pst I, Pvu II and Hinc II were also similar.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2005년도 생물공학의 동향(XVI)
• /
• pp.197-197
• /
• 2005

• 한국미생물학회:학술대회논문집
• /
• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
• /
• pp.76-77
• /
• 2005

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
• /
• pp.330.2-330
• /
• 1998

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
• /
• pp.331.1-331
• /
• 1998

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
• /
• pp.335.1-335
• /
• 1998

No Abstract, See Full Text

• 한국미생물생명공학회:학술대회논문집
• /
• 한국미생물생명공학회 2002년도 학술발표대회
• /
• pp.85-85
• /
• 2002

• 한국미생물학회:학술대회논문집
• /
• 한국미생물학회 2009년도 International Meeting of the Microbiological Society of Korea
• /
• pp.119-120
• /
• 2009