• Fisheries and Aquatic Sciences
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• 제5권4호
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• pp.308-310
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• 2002

Effect of 4-nonylphenol (4-NP), endocrine disrupting compounds (EDCs), on glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) were investigated in the plasma of juvenile rockfish, Sebastes schlegeli. Fish were injected with 4­NP (10, 50, 100 and 200 mg/kg body weight) in $70\%$ ethanol twice at 3-day intervals and plasma sampling were extracted at 7 days after the last injection. Controls received solvent only. 4-NP significant increased GOT in a dose-dependent manner. GPT was markedly elevated to $61\%$ (P<0.05) and $82\%$ (P<0.01) than that of the control at the 4-NP doses of 100 and 200mg, respectively. These results suggest that the estrogenic activity of 4-NP increase plasma GOT and GPT by toxic effect on hepatocyte.

• 한국식품영양과학회지
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• 제29권2호
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• pp.193-197
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• 2000

The effects of black soybena extracts on enzymes activies of rat were evaluated in present study. Eighty-four male Sprague-Dawley rats weighing 100$\pm$10g were divided into twelve groups which consisted of black soybean extract, Pb and Cd solution, and black soybean extract plus Pb or Cd soln groups. The weight gain was increased in black soybean extracts and Pb soln solution group but decreased in Cd soln solution group. The results obtained form the experiment were as follows: Glutamate pyruvate trasaminase (GPT) and glutamate oxaloacetate oxaloacetate transaminase (GOT) activities were not significantly different among experimental groups. The lactate dehydrogenase (LDH) activities of black soybean extract administered groups were decreased than those of Pb and Cd solution group. Black soybean group increased cholinesterase (ChEase) activity as compared to administration of Pb and Cd soln group.

• The Korean Journal of Physiology
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• 제26권1호
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• pp.89-97
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• 1992

In hypoxic tissue conditions, pyruvate can not enter the Krebs cycle and lactic acid, produced from pyruvate, accumulates to induce lactic acidosis. Pyruvate, However, can also be converted to alanine by glutamate pyruvate transaminase, that could be enhanced by glutamate. Therefore, it would be a fundamental measure to treat the lactic acidosis in tissue hypoxic conditions when one can convert the accumulated lactic acid, through pyruvate, to alanine. To test the above hypothesis, we induced a lactic acidosis in cats and the effect of glutamate on recovery of acid base state and removal of the lactic acid from blood were assessed and the results were compared with those of bicarbonate administration, which is one of the most frequently used conventional measure for correction of the acid base state during lactic acidosis. The results were that glutamate and combined glutamate bicarbonate solutions not only restored the acid base status completely from the lactic acidosis in an hour or two, but also restored the blood level of lactate partially. We concluded that administration of glutamate solution to convert pyruvate into alanine is effective in preventing lactic acid accumulation and treating lactic acidosis.

• Biomolecules & Therapeutics
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• 제4권3호
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• pp.271-274
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• 1996

The antihepatotoxic activity of Bezoar Bovis and Moschus was investigated by in vitro assay method using galactosamine and carbon tetrachloride-induced cytotoxicity in primary-cultured rat hepatocytes. The antihepatotoxic activity was evaluated by measuring the level of glutamate pyruvate transaminase and sorbitol dehydrogenase which were released from the necrotic hepatocytes to the culture medium. In galactosamine-intoxicated hepatocytes, the chloroform fraction of Bezoar Bovis reduced the level of glutamate pyruvate transaminase and sorbitol dehydrogenase resulting in 65% and 59% protection, respectively. The n-Hexane fraction of Moschus resulted in 45% and 40% protection, respectively in this system. In the case of carbon tetrachloride-intoxicated rat hepatocytes, Bezoar Bovis did not have significant effect and only the aqueous fraction of Moschus showed 42% and 40% protection, respectively.

• 대한한의학회지
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• 제23권2호
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• pp.139-150
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• 2002

OBJECTIVES : Schizandra chinensis is well known for its efficacy at liver function reinforcement, relieving thirst and recovery from fatigue. In this study, we examined the effects of sports drink containing Schizandra chinensis on serum metabolic substrate, electrolyte, stress indicators, related-enzyme and exercise performance, rectal temperature, and heat shock proteinb70 (HSP70). METHODS : Elite long-distance runners (male, 21.3yrs, n=16) were selected and divided into two groups; an experimental group (EXP, n=8) and a control group (CON, n=8). A beverage containing Schizandra chinensis was supplemented 3 times per day to EXP for 4 weeks. Serum biochemical elements (glucose, lactate, total cholesterol, triglyceride, high density lipoprotein cholesterol, glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, creatinine, creatine phosphokinase, lactate dehydrogenase, blood urea nitrogen, Na, K, Cl) were analyzed by auto blood analyzer. Exercise performance was measured by treadmill exercise test, HSP70 was detected by electrophoresis and Western blotting, and rectal temperature was measured by rectal temperature probe. RESULTS : Administration of the beverage increased significantly the rest level of blood Na, Cl and glucose and decreased significantly lactate dehydrogenase, glutamate oxaloacetate transaminase. No difference was found in exercise performance, rectal temperature increment or HSP70 concentration between groups. CONCLUSIONS : Administration of a sports drink containing Schizandra chinensis altered blood glucose, lactate dehydrogenase, glutamate oxaloacetate transaminase, Na and Cl levels.

• 한국패류학회지
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• 제27권4호
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• pp.323-330
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• 2011

동해안 일대의 양식자원 증식과 어업인의 소득증대를 위해 남해안 일대에서 주를 이루고 있는 전복양식을 동해안 일대로 넓히기 위해서 수심이 깊고 파고가 높은 동해안 지역에 맞는 전복과 해삼 복합사육기를 개발하고 복합사육기의 효과를 알아보고자 양식 생산력을 조사하였다. 전복해삼복합사육기에 각장 5 cm 전복 500마리와 7-9 cm 해삼을 50, 75, 100마리로 각각 넣어 실험한 결과 해삼 적정수용능력은 13-18마리/$m^2$로서 사육기당 52-72마리가 적절하였다. 수용량에 따른 생화학적 건강도를 조사하기 위해서 복합사육기에 각장 5 cm 전복 300마리와 500마리를 각기 달리 수용하고, Reflotron kit를 이용하여 GOT (glutamate oxaloacetate transaminase) 와 GPT (glutamate pyruvate transaminase) 를 조사하였으며, 전복과 해삼을 함께 사육하여 RNA/DHA ratio와 DNA 및 RNA contents (ug/mg) 를 조사한 결과, 전복 단독구와 복합사육구간에 유의적인 차이가 없어 (P > 0.05) 전복과 해삼을 함께 양성하더라도 해삼이 전복 성장에 영향을 미치지 않는 것으로 나타났다. 또한, 자체 개발한 전복해삼 복합사육기와 남해안에서 주로 사용되고 있는 전복 사육기의 생산능력을 비교하기 위해서 수하식으로 8개월 동안 실험한 결과, 자체 개발한 복합사육기에서 양식한 전복과 해삼이 성장이 빨라 전복해삼복합사육기가 동해안 일대의 양식사업에 적합한 것으로 나타났다.

• Environmental Analysis Health and Toxicology
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• 제17권3호
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• pp.239-243
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• 2002

Changes of plasma vitellogenin (VTG) and glutamate pyruvate transaminase (GPT) were examined for determining whether hepatocyte was damaged during the process of VTG induction in the juvenile rockfish, Sebastes schlegeli exposed to exogenous estrogen (estradiol-l7$\beta$, E$_2$). Rockfishes were intraperitoneally injected with E$_2$(5 mg/kg B.W.) in 70% ethanol and plasma sampling were extracted at 0, 1, 3, 6, 9, 12, 15 days af-ter E$_2$administration. VTG and GPT were then analyzed by SDS -PAGE and Reitman -Frankel method, respectively. VTG band was detected at a molecular weight position of 175 kDa on Day 3 after E$_2$administration. This band became more distinct at 6 days, but its was gradually thinned with time -course, and not detected at 15 days. GPT was suddenly increased at 1 days after 22 administration and highest GPT was detected at 3 days. However. GPT was gradually decreased with time -course as the change of VTG. These results suggest that the process of VTG induction by exogenous E$_2$damage to hepatocyte, and plasma GPT was temporarily increased in the juvenile rockfish.

• Natural Product Sciences
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• 제9권2호
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• pp.83-86
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• 2003

In the present study, Thespesia populnea (Malvaceae) bark was extracted with methanol and water. The extracts were vacuum dried to yield the respective methanol (MET) and aqueous extract (AET). The extracts were evaluated for hepatoprotective activity against carbon tetrachloride $(CCl_{4})$ induced liver damage at 2 dose levels (250 and 500 mg/kg). The biochemical parameters observed in serum were total bilirubin, alkaline phosphatase (ALP), serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) levels and total protein. Aspartate transaminase (AST), alanine transaminase (ALT) and total protein levels in liver were also evaluated. Histopathological study on the liver tissue was also performed. The extracts exhibited dose dependent reduction in total bilirubin, ALP SGOT, SGPT, AST, ALT and increase in total protein (serum and liver) levels. The extracts also exhibited only mild hepatocytic damage compared to the $CCl_{4}$ Treated group. MET was found to exhibit higher hepatoprotection than AET.

• Natural Product Sciences
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• 제14권2호
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• pp.95-99
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• 2008

In a project to study the hepatroprotective effect of some plant extracts four plants Ephedra foliate Boiss, Alhagi maurorum Medikus, Capsella bursa-pastoris (L.) Medik. and Hibiscus sabdariffa L. were studied. The ethanol extract of the aerial part of the first three plants and the flowers of H. sabdariffa were subjected to hepatoprotective assays using Wistar albino rats. Liver injury induced in rats using carbon tetrachloride. The biochemical parameters; serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP) and total bilirubin were estimated as reflection of the liver condition. Based on the good results of the biochemical parameters measurements, histopathological study was performed on the liver of rats treated with E. foliate. The normal appearance of hepatocytes indicated a good protection of the extract from carbon tetrachloride hepatotoxicity. All the results were compared with silymarin, the reference hepatoprotective drug.

• 한국임상수의학회지
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• 제17권1호
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• pp.28-31
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• 2000

An animall health monitoring system in Gyeongnam area(near Chinju) was studied to analysis of biochemical risk factors in 617 herds. Clinical serum factors such as glutamate oxaloacetate transaminase(GOT), glutamate pyruvate transaminase(GPT), Ca, P, Mg, glucose, and cholesterol were measured with automatic biochemical analyzer(Ra-X7T Techmmicon, USA). In serum analysis, 613 cattle were within normal llimits(GOT: 9.5-85 IU-dl, GPT: 25-77IU/dl, total protein: 5.8-8.5g/dl, Ca: 4.2-12.4mg/dl, P: 4.6-9.7mg/dl, Mg: 1.5-3.0mg/dl, glucose: 48-120mg/dl, Cholesterol: 70-170mg/dl), the other cattle showed high glucose and high cholesterol level. It is proposed that clinical serum factors to be estimated may be valuable for developing of animal health monitoring system model.