• Microbiology and Biotechnology Letters
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• v.39 no.1
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• pp.9-19
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• 2011

The Bacillus subtilis pyrimidine biosynthetic (pyr) operon encodes all of the enzymes for the de novo biosynthesis of Uridine monophosphate (UMP) and additional cistrones encoding a uracil permease and the regulatory protein PyrR. The PyrR is a bifunctional protein with pyr mRNA-binding regulatory funtion and uracil phosphoribosyltransferase activity. To study the global regulation by the pyrR deletion, the proteome comparison between Bacillus subtilis DB104 and Bacillus subtilis DB104 ${\Delta}$pyrR in the minimal medium without pyrimidines was employed. Proteome analysis of the cytosolic proteins from both strains by 2D-gel electrophoresis showed the variations in levels of protein expression. On the silver stained 2D-gel with an isoelectric point (pI) between 4 and 10, about 1,300 spots were detected and 172 spots showed quantitative variations in which 42 high quantitatively variant proteins were identified. The results showed that production of the pyrimidine biosynthetic enzymes (PyrAA, PyrAB, PyrB, PyrC, PyrD, and PyrF) were significantly increased in B. subtilis DB104 ${\Delta}$pyrR. Besides, proteins associated carbohydrate metabolism, elongation protein synthesis, metabolism of cofactors and vitamins, motility, tRNA synthetase, catalase, ATP-binding protein, and cell division protein FtsZ were overproduced in the PyrR-deficient mutant. Based on analytic results, the PyrR might be involved a number of other metabolisms or various phenomena in the bacterial cell besides the pyrimidine biosynthesis.

• Journal of Internet Computing and Services
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• v.18 no.6
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• pp.137-144
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• 2017

A variety of customized marketing strategies are being implemented in the telecommunications market, including the offering of gifts to consumers, as competition in the market is being restructured with the focus on bundled products. However, the Korea Communications Commission (KCC) has recently imposed fines for user discrimination on telecommunication carriers whose marketing strategies have included the award of excessive prizes. In that regard, various issues related to the suitability and appropriate limit of the current gift regulation in the telecommunications market have been raised. Since the Fair Trade Commission, which had a similar scheme to that of the KCC, abolished the regulation on gifts in July 2016, disputes have arisen as to whether the current regulation on gifts is practically effective from the perspective of consumer benefit. In other words, discussions on the rationality of the theoretical and empirical grounds for the regulation on gifts only in the telecommunication market but not in other commodities markets have begun. As such, this study aims to empirically analyze the suitability of the current regulation on gifts in the domestic telecommunications market under the new competitive environment centered on bundled products, and to seek the rational improvement of, and infer the implications for, the regulation on gifts in the telecommunications market based on the results of the analysis.

• Journal of Korean Society of Environmental Engineers
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• v.38 no.8
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• pp.435-443
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• 2016

In this study, MACT (Maximum Achievable Control Technology) application effect was evaluated for minimization of benzene fugitive emission in petrochemical industry. Although fugitive emission for benzene in the nation was regulated by the Clean Air Conservation Act from 2015, the US EPA already has introduced MACT standard to minimize its emission with up-to-date technology since 1995. EPA Emission Factor (AP-42) and EPA MACT Standard Guideline were used to assess MACT application effect. As a result, For MACT application it could reduce benzene emission up to 98% (average) comparing with uncontrolled facility, while the national regulation could achieve about 95% (average) reduction which is slightly lower than MACT. However there is no control measure in the national regulation to reduce benzene emission for vessel loading even though MACT standard requires preventive facility such as VRU (Vapor Recovery Unit). For further reduction of benzene emission, it needs to be mandatory for operation of VRU when benzene product is loaded in vessel. These efforts could contribute to achieve the global level for benzene emission management in national petrochemical industry.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.100.2-101
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• 2003

The global regulator, GacS (global antibiotic and cyanide sensor kinase), was required for the increased resistance to hydrogen peroxide occurring as cultures of the rhizobacterium, P. chlororaphis O6, matured. Specific stationary-phase peroxidase and catalase isozymes were absent in the GacS mutant, whereas a manganese-superoxide dismutase isozyme was expressed earlier and to a great extent than wild type. In the wild type cell, transcript accumulation of rpoS was higher in late logarithmic-phase cells than cells from mid logarithmic- or stationary-phase. Transcripts from rpoS in the GacS mutant were reduced in each of these growth phases compared to the wild type expression. The down stream sequence from rpoS lacked sequences encoding a small RNA, rsmZ, found in other pseudomonads and implicated in control of genes activated by the GacS system. These findings suggest that GacS-mediated regulation of RpoS plays role in control of oxidative stress in P. chlororaphis O6 by as yet an unknown mechanism.

• Journal of Information Technology Services
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• v.10 no.3
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• pp.31-47
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• 2011

With the saturation of the local market, the domestic media industry is searching for new growth drivers by crossing over to other industries, but due to the limited size of the market, results fail to meet expectations. Despite its strengths in cultural contents, as verified by the Korean Wave Hallyu, and advanced platform services, the industry feels threatened by the entry of global foreign media businesses in the domestic arena. This and the stagnated growth in the local media market are challenges we are facing today. On the other hand, it could prove to be a timely opportunity to transform the existing media industry structure where exporting hardware and terminals is at the heart of a globalized business into a structure based on software and contents exports. Based on such critical thinking, this study examines the current status of media markets, home and abroad, and aims to come up with strategic measures through a discussion on the issues and methods proposed for the globalization of local media businesses from a holistic viewpoint based on ecosystem, M&A and contents.

• Research in Plant Disease
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• v.23 no.3
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• pp.212-227
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• 2017

Pseudomonas chlororaphis O6, isolated from the roots of dryland, field-grown commercial wheat in the USA, enhances plant health and therefore it is used in agriculture as a biofertilizer and biocontrol agent. The metabolites produced by this pseudomonad stimulate plant growth through direct antagonism of pathogens and by inducing systemic resistance in the plant. Studies upon P. chlororaphis O6 identify the pathways through which defined bacterial metabolites generate protection against pathogenic microbes, insects, and nematodes. P. chlororaphis O6 also triggers plant resistance to drought and salinity stresses. The beneficial determinants are produced from bacterial cells as they form biofilms during root colonization. Molecular control these processes in P. chlororaphis O6 involves the global regulatory Gac/Rsm signaling cascade with cross-talk between other global regulatory pathways. The Gac/Rsm regulon allows for coordinate phasing of expression of the genes that encode these beneficial traits among a community of cells. This review provides insights on the Gac/Rsm regulon in expression of beneficial traits of the P. chlororaphis O6 which can contribute to help yield enhancement and quality in agricultural production.

• YAKHAK HOEJI
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• v.59 no.3
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• pp.113-119
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• 2015

Protein phosphorylation is one of most important post-translational modifications (PTMs) and plays an important role in regulation of protein function. Here we develop a method for a global identification of phosphopeptides and phosphorylation sites using nano-LC MS/MS. We compared two separation methods, C4 and strong cation ion exchange (SCX). Before phosphopeptides enrichment with $TiO_2$, total proteins from Rat 1 cells have been separated using C4 column or tryptic peptides of proteins from the cells have been separated using SCX column. Finally, we have detected 52 phosphorylation sites on 41 proteins from SCX method and 375 phosphorylation sites on 252 proteins from C4 method, and determined the function and localization of identified phosphoproteins using DAVID software. In particular, we showed new phosphorylation sites from membrane proteins related to various cell signaling mechanisms. This method may contribute to study global signal networks induced by various signals including ligands and drugs.

• Journal of Korea Society of Digital Industry and Information Management
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• v.8 no.4
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• pp.217-230
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• 2012

To look at Korea trade environment, as increasing the quantity of goods transported in North-East Asia and deepening national competitive, information-oriented logistics environment is needed. That means it is automated work process and can provide value-added service. To do this, logistics business can be efficiently handled through "Electronic Means for the Clearance of Ships" and electronically data processing. Also, by increasing the risk of international terrorism, regulation regarding to logistics security has strengthened around the advanced country, such as USA and EU, etc. or international organization, such as IMO, ISO, etc.. Furthermore, because trade and logistics environment is changing and security becomes more important in logistics area, movement or status information of goods within the port or in entry in/out becomes more and more be needed in real time. In case cross-border logistics activities, international cooperation or agreement between countries is very important. However, different opinions much happened on defining the interface. If to follow one format or type using at a specific country or organization, it was occurred to difficulty in respect of interoperability as well as additional costs or time. Therefore, this paper proposes common interface model to exchange information for the efficient global SCM for using by the output of Korea R&D project, "National Logistics Standardization System Development".

• Journal of Korea Society of Digital Industry and Information Management
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• v.16 no.3
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• pp.73-82
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• 2020

'ISO 37001 - Anti-bribery Management Systems, one of the Business Ethics of Global Standards, is a new standard to help organizations fight bribery and promote an ethical business culture which was developed in 2016. ISO 37001 can provide the tools and systems to greatly reduce the risk and help organizations deal with it effectively if it does arise. After enforcing the Improper Solicitation and Graft Act, Korean government standardized ISO 37001 harmonizing International Standards into Korean Industrial Standards (KS) in 2017. It is meaningful in that Korean companies have localized regulation which applies to global standard anti-bribery management systems. About 100 organizations have adopted ISO 37001 as in the first half of 2019. So far many side effects of other ISO certifications have been recognized. Therefore, it is necessary to investigate the current status of ISO 37001 in Korea for effective operation and management. The primary purpose of this paper is to investigate the current status and problems of ISO 37001 in Korea. It can provide useful guidelines for improving ISO 37001 to manage bribe and corruption effectively.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.8_spc
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• pp.1219-1232
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• 2019

The global goat population continues to grow and is now over one billion. The number of goats raised primarily for milk production is also growing, due to expanding demand. Most of the world dairy goat production and consumption is in Asia, but a global view of the dairy goat sector reveals important lessons about building successful modern dairy goat industries. The most organized market for goat milk is found in Europe, especially in France. The European goat sector is specialized for milk production, mostly for industrial cheesemaking, while also supporting traditional on-farm manufacturing. Government involvement is significant in sanitary regulation, research, extension, support for local producer organizations, and markets, and ensures safety and quality. Nonetheless, producers are still vulnerable to market fluctuations. New dairy goat industries are developing in countries without a long goat milk tradition, such as China, the United States, and New Zealand, due to rising consumer demand, strong prices, and climate change. The mix of policies, management and markets varies widely, but regardless of the country, the dairy goat sector thrives when producers have access to markets, and the tools and skills to sustainably manage their livestock and natural resources. These are most readily achieved through strong and inclusive producer organizations, access to technical services, and policies that enable the poor and marginalized groups to benefit from increasing demand.