• Korean Journal of Microbiology
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• v.49 no.3
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• pp.297-300
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• 2013

EPS producing bacteria were enumerated in ginseng root system (rhizosphere soil, rhizoplane, inside of root). EPS producing bacterial density of rhizosphere soil, rhizoplane and inside of root were distributed $9.0{\times}10^6$ CFU/g, $7.0{\times}10^6$ CFU/g, and $1.4{\times}10^3$ CFU/g, respectively. Phylogenetic analysis of the 24 EPS producing isolates based on the 16S rRNA gene sequences, EPS producing isolates from rhizosphere soil (RS) belong to genus Arthrobacter (6 strains) and Rhizobium (1 strain). EPS producing bacteria from rhizoplane (RP) were Arthrobacter (6 strains), Rhodococcus (1 strain) and Pseudomonas (1 strain). EPS producing bacteria from inside of root (IR) were categorized into Rhzobium (6 strains), Bacillus (1 strain), Rhodococcus (1 strain), and Pseudomonas (1 strain). Phylogenetic analysis indicated that Arthrobacter may be a member of representative EPS producing bacteria from ginseng rhizosphere soil and rhizoplane, and Rhizobium is typical EPS producing isolates from inside of ginseng root. The yield of EPS was 10.0 and 4.9 g/L by Rhizobium sp. 1NP2 (KACC 17637) and Arthrobacter sp. 5MP1 (KACC 17636). The purified EPS were analyzed by Bio-LC and glucose, galactose, mannose and glucosamine were detected. The major EPS sugar of these strains was glucose (72.7-84.9%).

• The Plant Pathology Journal
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• v.33 no.3
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• pp.337-344
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• 2017

To develop a commercial product using the mycoparasitic fungus Simplicillium lamellicola BCP, the scale-up of conidia production from a 5-l jar to a 5,000-l pilot bioreactor, optimization of the freeze-drying of the fermentation broth, and preparation of a wettable powder-type formulation were performed. Then, its disease control efficacy was evaluated against gray mold diseases of tomato and ginseng plants in field conditions. The final conidial yields of S. lamellicola BCP were $3.3{\times}10^9conidia/ml$ for a 5-l jar, $3.5{\times}10^9conidia/ml$ for a 500-l pilot vessel, and $3.1{\times}10^9conidia/ml$ for a 5,000-l pilot bioreactor. The conidial yield in the 5,000-l pilot bioreactor was comparable to that in the 5-l jar and 500-l pilot vessel. On the other hand, the highest conidial viability of 86% was obtained by the freeze-drying method using an additive combination of lactose, trehalose, soybean meal, and glycerin. Using the freeze-dried sample, a wettable powder-type formulation (active ingredient 10%; BCP-WP10) was prepared. A conidial viability of more than 50% was maintained in BCP-WP10 until 22 weeks for storage at $40^{\circ}C$. BCP-WP10 effectively suppressed the development of gray mold disease on tomato with control efficacies of 64.7% and 82.6% at 500- and 250-fold dilutions, respectively. It also reduced the incidence of gray mold on ginseng by 65.6% and 81.3% at 500- and 250-fold dilutions, respectively. The results indicated that the new microbial fungicide BCP-WP10 can be used widely to control gray mold diseases of various crops including tomato and ginseng.

• Korean Journal of Medicinal Crop Science
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• v.20 no.2
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• pp.124-128
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• 2012

When ginseng seedlings are cultured in paddy fields, quality degradation and yield reduction are induced by severe plant loss with chlorosis on leaves occurred physiological disorder by excessive salt and poor drainage, rusty-root occurrence, and root rot etc. Accordingly, in order to solve these problems, this study was performed to investigate the treatment method, concentrations and time of activated ion calcium as environment-friendly agricultural materials. Activated ion calcium is an enriched and purified water-soluble mineral calcium component for absorbing quickly into plant as a highly functional calcium and it is an alkaline calcium of 37% (370 $m{\ell}$/1 ${\ell}$) concentration with pH 13. Treatment method was that ginseng seeds were sown after removing water in the shade after seed immersion for 1 minute with active ion calcium of 20-fold diluted solution, and then irrigated $4{\ell}$ per 3.3 $m^2$ with 200-fold, 400-fold, and 600-fold diluted solution before emergence on late March, and supplied 1 time on leaves with 500-fold diluted solution in June and July respectively. The disease rate by treatment of activated ion calcium was that on the treatment of soil irrigated with 200-fold diluted solution compared to non-treated soil, damping-off was 33%, anthracnose was 100% reduced and the occurrence rate of rusty-root was 30% reduced. In addition, when active ion calcium of 200-fold diluted solution were soil irrigated, first and second grade ginseng were respectively 26% and 22% produced more, compared with control.

• Journal of the Korea Society for Simulation
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• v.30 no.2
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• pp.41-48
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• 2021

As the rural population continues to decline and aging, the improvement of agricultural productivity is becoming more important. Early prediction of crop quality can play an important role in improving agricultural productivity and profitability. Although many researches have been conducted recently to classify diseases and predict crop yield using CNN based deep learning and transfer learning technology, there are few studies which predict postharvest crop quality early in the planting stage. In this study, a early quality prediction model is proposed for sprout ginseng, which is drawing attention as a healthy functional foods. For this end, we took pictures of ginseng seedlings in the planting stage and cultivated them through hydroponic cultivation. After harvest, quality data were labeled by classifying the quality of ginseng sprout. With this data, we build early quality prediction models using several pre-trained CNN models through transfer learning technology. And we compare the prediction performance such as learning period and accuracy between each model. The results show more than 80% prediction accuracy in all proposed models, especially ResNet152V2 based model shows the highest accuracy. Through this study, it is expected that it will be able to contribute to production and profitability by automating the existing seedling screening works, which primarily rely on manpower.

• Journal of Ginseng Research
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• v.26 no.2
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• pp.89-95
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• 2002

We screened biotic and abiotic preventative,i(preventers) from natural resources to prevent the rusty phenomenon in ginseng roots. To select preventatives(preventers), soil microbes such as Agrobacterium and certain microbes isolated from the rusty ginsengs and the soil in which the rusty ginsengs were planted and used. It is also performed with germination tests of the seeds of Latuca Sativa L. We identified that how selected preventatives(preventers) effect the germination of ginseng seeds. Furthermore, how these influence on the rusty phenomenon and the growth of 1 -year-old ginsengs treated in the pavement. The final preventatives; ICPE-C1$\sub$05/, ICPE-P$\^$107/ were effective in not only the growth of ginseng, but also inhibition of the rusty phenomenon. Moreover, we selected abiotic soil improvers; called P, R, and W, respectively; to promote the effects of preventatives. R and W was excellented among choring improvers. The germination rate of 2-year-old ginsengs treated with ICPE-C$\sub$105/P, and ICPE-P$\sub$107/P was the highest under the effects of naturally selected preventatives mixing with abiotic soil improvers. All treat which was compounding preventers & improvers were so excellented of growth ginseng. Especially treats of ICPE-C$\sub$105/R and ICPE-P$\sub$107/R showed growth increased of each 67.3% and 52.7% As well, the growth of ginseng was the highest in the treatment of ICPE-C$\sub$105/R, and ICPE-P$\sub$105/R. Though rusty of rate was emerged 35% in control, preventers ICPE-C$\sub$105/R and ICPE-P$\sub$107/R were emerged 5.3%. It was affirmed effective of preventer. On the other hands, amounts of ginsenoside treated with preventatives showed to be changed. The ginsenoside was increased to 14.2% with treatment with ICPE-P$\sub$107/R which is highest among groups compared to control, and ICPE-C$\sub$105/P was increased to 5.0%. To sum up with total results, it is judged that biotic preventatives (ICPE-C$\sub$105/R, and ICPE-P$\sub$107/R) which we created improve both a high yield of ginseng and the inhibition of the rusty phenomenon. phenomenon.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.4
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• pp.285-289
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• 1989

Interrelationships between yields of Burley 21 variety and meteorological factors such as precipitation, duration of sunshine, and evaporation in Suweon, Chungju, Daegu, and Jeonju were investigated during the growing seasons from 1976 to 1985. Cultivation period from the 41st to the 60th day after transplanting which showed the highest transpiration by the tobacco plant was corresponding to relatively dry season in Korea from the middle of May to early of June. The meteorological factors during the middle stage of the tobacco growth, especially the 41st to the 80th day after transplanting, seemed to have higher correlation with tobacco yield, than any other periods. This result implied that the middle stage of the growth when tobacco plant consume more soil moisture by transpiration proved to be a important growth stage having a great influence upon tobacco productivity.

• Journal of the Korean Society of Tobacco Science
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• v.13 no.2
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• pp.66-70
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• 1991

A field experiment was conducted to study the effects of fertilizer application rate and number of harvested leaves per plant on selected agronomic, chemical and physical characteristics of burley tobacco(Nicotiana tabacum L. cv Burley 21) in 1988, 1989 and 1990 at Chonju Experiment Station, Korea Ginseng SE Tobacco Research Institute. 175.0 or 227.5 kilogram per 10a of compound fertilizer(N-P2O5-K2O= 10-10-20) were applied as main plot, and 2 or 4 of upper leaves was topped off with 0, 2 or 4 of the lower leaves removed as sub plot. Yield, total alkaloid and total nitrogen content of cured leaves were high in the higher level of compound fertilizer plot, and increasing the level of compound fertilizer had an adverse effect on physical characteristics. Reducing the number of harvested leaves had a negative effect on yield regardless of whether the leaf number was reduced by lower topping or removing the bottom leaves, but increased the rate of higher grades leaves. Differences in total alkaloid, total nitrogen and some physical properties among number of harvested leaf per plant were smaller than expected.

• Journal of the Korean Society of Tobacco Science
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• v.11 no.2
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• pp.149-155
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• 1989

This study was conducted to obtain the breeding information for the leaf breakdown, and correlation coefficients between leaf breakdown and some agronomic characters in flue-cured tobacco at Taegu Experiment Station, Korea Ginseng & Tobacco Research Institute. NC 95 and NC 2326 were used as parents. The bulk method procedure was used from the F2 to the F4 generations, 54 plants were selected among F4, and 11 lines were selected among 54 F5 which based on high resistance to bacterial wilt compared with NC2326. The entries were composed of parents and 11 F6 advanced lines, and designed in randomized block with 3 replications. Plant seedlings were transplanted in 16 April. The number of breakdown leaf were investigated at flowering stage, 18 June. There were significant difference between NC 95 and NC 2326 for the number of breakdown leaf, number and weight of ground suckers, days to flower, yield and resistance to bacterial wilt, and similar results were appeared among lines. Estimated heritability in the broad-sense ranged from 59.3% to 87.4% for all characters. Most of genetic correlations were higher than phenotypic coefficients between characters. Number of breakdown leaf was positively correlated with days to flower, the number and weight of ground suckers, while it was negative to yield. One line among 11 had few breakdown leaf, low producted ground suckers and high resistance to bacterial wilt. Above results suggest that the number and weight of ground suckers can be use as a selection criterion for leaf breakdown even under the condition without leaf breakdown.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.4
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• pp.544-548
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• 2005

The objective of this study was to determine the effect of die temperature and dimension on extraction pattern, extract yield, and crude saponin content of extruded white ginseng. The extrusion variables were die temperature $(110\;and\;120^{\circ}C)$ and die dimension (3 holes with 1.0 mm, 2 holes with 2.0 mm, and 1 hole with 3.0 mm diameter). The browness and redness were indicator of active components in ginseng extract. Both were used to evaluate the effect of die temperature and die dimension on release pattern and release rate constant. Browness and redness of extract achieved its lowest value at die temperature $110^{\circ}C$ and 2 holes with 2.0 mm diameter, indicating the lowest extraction rate constant. Extract yield highly increased by extrusion treatment. Extract yield and crude saponin content were the highest at die temperature $120^{\circ}C$ and 1 hole with 3.0 mm diameter. In conclusion, extrusion process has contributed significantly in improvement of release rate of its active components.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.3
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• pp.274-279
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• 1983

In order to find out the application effect of commercial organic fertilizer on the germination rate of ginseng seed, growth of ginseng plant and changes of the physicochemical properties of soils, a Miwon organic fertilizer, byproduct of amino acid fermentation, was applied to the ginseng nursery bed. The application rates of Miwon organic fertilizer were 0kg-N from organic fertilizer and 30kg-N from wild grass compost per 10a as control(namely 0kg-N/10a), 2.5kg-N and 27.5kg-N/ 10a(2.5kg-N/10a) 5.0kg-N and 25kg-N/10a(5.0kg-N/10a), 10kg-N and 20kg-N/10a(10kg-N/10a), 15kg-N and 15kg-N/10a(15kg-N/10a) respectively, The obtained results are as follows: 1. The germination rate of ginseng seed were better in the plots that received 2.5kg, 5.0kg and 10kg-N/10a as compared with control plot. However no difference was found on germination rate between control and 15kg-N/10a, 2. The higher rate of missing plant was found in the 15kg-N/10a plot that showed poor growth. However control and 10kg-N/10a plot showed similar rate of missing plant. The best result, lower rate of missing plant, was obtained in the order of 5kg-N and 2.5kg-N/10a respectively. 3. The significant heavier ginseng root weight was obtained in 5kg-N/10a and 10kg-N/10a plot. However the root weight was decreased in the order of 2.5kg-N/10a, control, 15kg-N/10a. 4. In the physicochemical changes of soils between control and organic fertilizer treated soil before and after harvesting the increment of pH, C.E.C. and organic matter content along with the rate of organic fertilizer application were found. There were significant correlation between nitrate nitrogen and the rate of organic fertilizer application. 5. In the view points of germination rate, missing rate of plant and yield of ginseng root, it can be stated that the best result could be obtained from the plot that received 10kg-N/10a from Miwon commercial organic fertilizer and 10kg-N/10a from wild grass compost.