• 대한치과교정학회지
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• 제30권4호
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• pp.423-431
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• 2000

교정적 치아이동 중 골개조는 수종의 매개물질에 의하여 조절된다. 특히 IL-$1\beta$는 실험동물의 교정적 치아이동 중 압박측과 긴장측 모두의 치근막과 치조골에서 골흡수를 촉진하고 골형성을 억제하는 효과를 보이는 것으로 알려져 왔다. 이 연구는 인체에서의 교정적 치아이동 중 압박측과 긴장측 치은열구액에서 IL-$1\beta$의 발현을 찾고 발현양의 시간적 변화를 관찰하였다. 전신질환인 없고 임상적으로 건강한 치주조직을 가진 평균 19.2$\pm$4.2세, 12명의 남녀환자에서 편측 견치를 원심으로 견인하였다. 실험군(원심 견인측)의 근심과 원심, 대조군(동일환자의 반대편 견치)의 근심 치은열구액을 견치 후방 이동 직전, 1시간 후, 24시간 후, 168시간 후의 4개의 시간대 별로 수집하여 총단백질 농도(BCA법)와 IL-$1\beta$(ELISA법) 의 농도를 비교, 관찰하여 다음과 같은 결과를 얻었다. 1. 실험군에서의 IL-$1\beta$ 농도는 교정력을 가한 후 증가하여 24시간 전후에 최대값에 도달하였다. 2. 대조군에서의 IL-$1\beta$ 농도는 시간에 따라 유의한 차이를 보이지 않았다. 3. 교정력을 가한 24시간 후에서 IL-$1\beta$의 농도는 압박측에서 가장 높고, 이어 긴장측과 대조군의 순이었다.

• Journal of Periodontal and Implant Science
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• 제23권3호
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• pp.391-398
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• 1993

Gingival crevicular fluid (GCF) is a promising source for markers of destructive periodontal disease activity. This study was undertaken to evaluate the protein composition of GCF in varying stages of the gingival inflammatory response. GCF sampled from 26 people with clinically healthy gingiva and 18 people with periodontitis were examined via sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS/PAGE). The result were as follows. 1. Total amount of GCF protein of diseased group significantly different from that of normal group. But difference in protein concentration was not that significant. 2. In analyzing GCF with SDS/PAGE, it was suggested that albumin is used as indicator plasma protein leakage because of heavily staining bond of albumin in patients with periodontal disease. 3. In diseased group, overall bonds of protein and bands of high molecular weight protein were heavily stained. It was proved useful information on high molecular plasma protein leakage with increasing vascular permeability due to inflammation.

• Journal of Periodontal and Implant Science
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• 제28권2호
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• pp.309-320
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• 1998

The periodontal health has been evaluated clinically by various epidemiological indices, and in researches by measurement of gingival crevicular fluid. Laser Doppler flowmetry is a reliable and objective method that allows immediate measurement of erythrocyte flux in approximately one cubic mm of the capillary bed without disturbing the tissues. The purpose of the present study was to determine whether human gingival blood flow was different according to measuring area, measuring time, and sex or not. Forty volunteers with good general and periodontal health, aged early twenties and unmarried, were selected. Laser Doppler flowmetry($floLAB^{(R)}$, Moor Instruments Ltd., England) was applied to measure the gingival blood flow of marginal gingiva, interdental papilla, attached gingiva and alveolar mucosa. The blood flow of interdental papilla was measured at 9-10 AM, 1-2 PM, and 5-6 PM. The difference of blood flow according to measuring area and measuring time was statistically analyzed by one way AOVA and Dunkan test, and the difference of blood flow between men and women was statistically analyzed by t-test. (1) Mean blood flow was significantly higher in alveolar mucosa than in the gingiva(p<0.05), and there was no significant difference in blood flow between marginal gingiva and interdental papilla(p>0.1). (2) Mean blood flow was significantly higher at 5-6 PM than at 9-10 AM and 1-2 PM(p<0.05). But there was no significant difference in gingival blood flow between 9-10 AM and 1-2 PM(p>0.1). (3) There was no significant difference in gingival blood flow between men and women(p>0.1). The above results suggest that the measurment of gingival blood flow using laser Doppler flowmetry may be clinically applicable to early determination of gingival inflammation and evaluation of healing status, but further studies are necessary to standardize and simplify the measuring procedure.

• 구강회복응용과학지
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• 제37권4호
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• pp.294-300
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• 2021

치태세균에 의해 야기되는 괴사성 치주질환은 감염부위에서 위막과 치간사이의 연조직 괴사 및 출혈, 촉진시 통증과 치은 출혈이 관찰된다. 환자들의 구내 세균을 조사해보면 방추균과 나선균이 혼합하여 존재하는 방추스피로헤타 세균총을 가지고 있다. 본 증례는 괴사성 치주질환으로 잠정 진단된 환자 2명을 대상으로 비외과적 치주치료, 3% 과산화수소를 적신 면봉을 이용한 드레싱 후 국소전달항생제를 도포하였고, 치료 전과 초기 치료 3 - 5일 후 치은열구액을 채득하여 실시간 연쇄중합반응법을 이용한 정량분석으로 구강내 미생물수를 측정하였다. 비외과적 치주치료 후 감염부위 치은이 정상으로 회복되었고, 7종 이상 검출되던 치주질환 관련 미생물도 1종만 검출되었다. 결과적으로 비외과적 치주치료를 통해 괴사성 치주질환 환자들의 치주 조직을 정상으로 회복하였다.

• Journal of Periodontal and Implant Science
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• 제29권3호
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• pp.577-594
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• 1999

The major cause of periodontal disease is microorganism in the dental plaque. Gingival sulcular fluid, which is exudate released from the tissue near crevicular epithelium is related with inflammation. The purpose of this study was to evaluate the argon laser efficiency between the clinical index and onset of collagenase of gingival sulcular fluid. Material divided 16 patients into 4 groups. The first control was without treatmemt. The second was with just treatment of argon laser, The third was treated by scaling and root planning and the fourth was treated with both scailing and root planning and argon laser. The level of periocheck test, the index of bleeding, and the depth of periodontal pocket were evaluated from for 128 teeth of 64 anterior teeth and 64 posterior teeth. The results were as follows ; 1. In the score of periocheck test, root planing group(group 3) was significantly reduced more than the group without treatment(group 1) and the argon laser treatment(group 2) for results of 3 days and 7 days. But root planing plus argon laser treatment(group 4) in the 7days after experiment, was significantly reduced than no treatment(group 1) and root planing treatment(group 3)(P<0.05), in the 3 days after experiment, was significantly reduced than root planing(group3)(P<0.05). The score of periocheck test to the root planning group(group 3) were significantly reduced between days1, day3 and day7(P<0.05). Root planning plus argon laser group(group 4) were significantly reduced to 1 or 7days and 3 or 7days(P<0.05). The argon laser group(group 2) didn't show any changes. 2. In the case of sulcus bleeding index, the root planning group(group 3) and root planning plus argon laser group(group 4) were reduced more than without treatment group(group 1)(P<0.05) and sulcus bleeding index in the root planning group(group 3) were reduced more than the argon laser group(group 2)(P<0.05). 3. There wasn't any changes of pocket depth between the control and the experiment group as with experiment periods also.