• Horticultural Science & Technology
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• v.33 no.6
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• pp.932-940
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• 2015

One of the main factors restricting production of triploid seedless watermelon is poor germination due to weak embryos, thick seed coats, and larger air spaces. This study was carried out to investigate the priming effects of different concentrations of chemicals, including hydrogen peroxide ($H_2O_2$), fusicoccin, and gibberellic acid (GA) on germination and seedling uniformity of triploid watermelon (Citrullus lanatus). Three commercial triploid cultivars, Seedless Plus, Sinus, and Sizero, were pretreated with water and different levels of $H_2O_2$ (2 and 4%), fusicoccin (FC: 1, 5, and $10{\mu}M$), and GA (1, 5, and $10{\mu}M$). The present findings helped to find optimal priming conditions for improving germination of triploid watermelon. Treatment with $5{\mu}M$ GA and hydropriming helped to break seed dormancy, enhancing the final germination percentages in all triploid cultivars and increasing the germination index in Sizero. These seed-priming treatments could be used on large scale for industrial applications. Moreover, hydropriming provides a simple, effective, and costless method to improve seed germination and seedling vigor of Sinus and Sizero varieties.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.217-217
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• 2017

Seed priming leading to improved emergences, germination speed and uniformity under unfavorable conditions without loss of longevity are the best level of techniques. We studied the possibility to obtain primed seed with reduced mean germination time (MGT) and uniformity but with simple, fast and effective methods. The experiments were carried out at $15^{\circ}C$ for 16 hours with priming treatment. To compare the effects of each treatment, we used 200mM $CaCl_2$ priming or hydro priming or no imbibition after ultrasonication or red light treatment. The light treatment was performed by irradiating 2000 lux of red light for 15, 30, 60, and 120 minutes. Addition, in order to investigate the effect of the priming solution, treated at 200mM $CaCl_2$ for 8 hours. Ultrasonication treatment was performed for 5, 10, and 20 minutes at exposures of 13.0, 17.3, and 21.6 kHz during priming. For liquid smoke (LS) treatment, seed soaked in 0%, 0.5%, 1.0%, 5.0% and 10.0% of diluted water and 200mM $CaCl_2$ solution. After each treatment, the seeds were dried to moisture content ranged 5-8% at $25^{\circ}C$ for 24 hours. The effect of seed treatment was evaluated with germination percentage (GP), MGT, germination index(GI), germination rate(GR), Germination Uniformity(GU) and heath seed percentage(HS). For several factors tested, we found that the desired germination improvement could be obtained by treating the seed with ultrasonication at 17.3 kHz for 5 minutes in water or red light exposure at 2000 lux for 120 min in water that resulted very similar to those used to 200mM $CaCl_2$ priming for 16 hrs. However, LS treatment showed no improvement in all diluted solution. Therefore, the methods applied ultrasonication and red light treatment showed high potential for fast and easy treatment avoiding pollution of salt solution waste.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.6
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• pp.507-512
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• 1993

Pre-sowing treatments of tobacco seeds (cv. NC82) were evaluated as a means of improving the speed of emergence, early seedling growth and uniformity of the seedlings. Seeds were treated through the use of polyethylene glycol 6000(PEG), solid matrix priming(SMP), chilling and dark-preincubati(DPI). Under the laboratory condition of 15$^{\circ}C$, all the treated seeds germinated more rapid and the time to 50% germination(T50) reduced than control. Among the treatments, SMP was the best to reduce the T50 for germination. All the treatments did not affect the final germination percentage, while the chilling showed of little benefit for germination and T50. Under the greenhouse condition, seedling from SMP emerged earlier and more uniformity and also greater mean fresh and dry weight showed than other treatments. The advantage of T50 from SMP was affected more in temperature than in water content of Agro-Lig during the treatment.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.213-213
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• 2017

High quality seed of proso millet that has high germination percentage, germination speed, and uniformity demanded to increases rates of mechanization in cereal crop cultivation. In order to improve germination characteristics, proso millet seeds were treated with red light, ultrasonication and liquid smoke (LS) solution that generated from hickory wood. All treatments were performed in seed priming solution with 100mM $NH_4NO_3$ at $15^{\circ}C$ for 24hrs under aeration condition. Seeds were exposed under light intensity of 2000 lux for 15m, 30m, 60m, and 120m in priming solution. Ultrasonic treatment was performed at 60%, 80%, and 100% intensity of 21.6 KHz for 5m, 10m, and 20m in priming solution. For LS treatment seed were soaked in 0%, 0.5%, 1.0%, 5.0% and 10.0% of diluted solution with $dH_2O$ or 100mM $NH_4NO_3$ solution. The effect of seed treatment was evaluated with germination percentage (GP), mean germination time (MGT), germination index (GI), germination rate (GR), Germination uniformity (GU) and heath seed percentage (HS). Our results demonstrate that red light (15min) or ultrasonication (21.6kHz, 5min) treatment improved MGT, GI, GR, and GU comparing to untreated control. Importantly, we show that LS treatments have significant effect on the health seedling and germination characteristics. Proso millet seeds that treated with 5% LS solution for 24hrs improves HS up to 97% that similar results obtained in 100mM $NH_4NO_3$ priming for 24hrs. The combined treatment with LS solution and 100mM $NH_4NO_3$ priming were not effective in all treatments. Our results demonstrate that treating seeds with LS or 100mM $NH_4NO_3$ priming or ultrasonication improves germination characteristics. The methods described here will help advance research using this species by increasing the germination performance at which successive seed pellet process.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.334-334
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• 2017

Unstable soil moisture conditions often negatively affect the emergence, seedling establishment, and growth uniformity at the initial stage, and then reduce the grain yield and biomass in direct seeding cultivation for rice in rainfed fields. Therefore it is important to develop a technique to increase the rapid and stabilized seedling establishment and improve the uniformity of initial growth after sowing. This study aims to confirm results on seed priming effect of indoor using petri dish experiments can be reproduced in the field using container at Ghana. Twenty-seven rice varieties including of Oryza sativa L. and O. glaberrima Steud. were used in this study. The experiments using petri dish and container with different soil moisture conditions (5%, 10%, 15%, 20%) were compared. As a result, a significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and emergence time uniformity in the primed seed of container in 10% and 15% soil moisture condition. A significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and plant height in the primed seed of container in 10% soil moisture condition in O. glaberrima. This study concluded that the priming effect in petri dish demonstrate those in container in the field condition of Ghana in 10% and 15% soil moisture condition during seedling stage.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.53-53
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• 2017

Unstable soil moisture conditions often negatively affect the emergence, seedling establishment, and growth uniformity at the initial stage, and then reduce the grain yield and biomass in direct seeding cultivation for rice in rainfed fields. Therefore it is important to develop a technique to increase the rapid and stabilized seedling establishment and improve the uniformity of initial growth after sowing. This study aims to confirm results on seed priming effect of indoor using petri dish experiments can be reproduced in the field using container at Ghana. Twenty-seven rice varieties including of Oryza sativa L. and O. glaberrima Steud. were used in this study. The experiments using petri dish and container with different soil moisture conditions (5%, 10%, 15%, 20%) were compared. As a result, a significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and emergence time uniformity in the primed seed of container in 10% and 15% soil moisture condition. A significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and plant height in the primed seed of container in 10% soil moisture condition in O. glaberrima. This study concluded that the priming effect in petri dish demonstrate those in container in the field condition of Ghana in 10% and 15% soil moisture condition during seedling stage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.1
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• pp.1-5
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• 1998

Experiments were carried out to find out the optimum water potential, temperature, and duration for the priming of rice seeds, Oryza sativa L. (cv. Ilpumbyeo) for better germination at sub-optimal temperatures. Seeds were primed in 0, -0.2, -0.4, -0.6, -0.8, and -1.0 MPa PEG (polyethylene glycol) solutions at $25^{\circ}C$. The optimum water potential for seed priming, the highest water potential at which rice seeds did not germinate, was -0.6 MPa. To find out optimum priming temperature and duration rice seeds were primed in -0.6 MPa PEG solution and 0 MPa (water as a control) for various durations at 15 and $25^{\circ}C$ and the seeds were germinated at 17, 20, and $25^{\circ}C$. Considering germination rate and speed, the optimum priming time in water (0 MPa) was 4 days at 15$^{\circ}C$ and 1 day at $25^{\circ}C$, while 4 days was the optimum priming time in a -0.6 MPa PEG solution, regardless of the priming temperature. Priming reduced the actual time of germination, especially at sub-optimal temperatures. Priming did not affect germination rate in -0.6 MPa PEG solution at 15$^{\circ}C$, but overpriming reduced the final germination rate in water at 15$^{\circ}C$ and in -0.6 PEG solution at $25^{\circ}C$. Total sugars and $\alpha$-amylase activity induced during the seed priming were negatively correlated with the final germination rate and there was no noted relationship with the speed or uniformity of germination.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.25 no.1
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• pp.1-10
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• 2022

This study was conducted to investigate the germination characteristics through the temperature change treatment compared to the general room temperature immersion treatment of the native species, the Larix kaempferi, and the Berula platyphyllavar. japonica seeds, and to obtain basic data for the production of healthy seedlings with high productivity and efficiency. As a result, the germination rate of the larch seeds showed a significant difference according to room temperature and temperature immersion treatment, and the average germination days of the birch seeds showed a significant difference according to room temperature immersion and temperature immersion treatment. On the other hand, there was no significant difference in germinal uniformity. The results of the study are as follows. First, the results of the analysis of the fallen leaves were the highest germination rate in the treatment of 1.5 days of hot water and 1.5 days of cold water, the fastest germination rate, the lowest average germination day, and the highest germination uniformity. This is the best result in most items, and the change temperature immersion treatment of hot and cold baths was superior to the conventional room temperature immersion treatment. Second, the results of birch tree showed that the germination rate was the highest in 1.5 days of hot water and 1.5 days of cold water. The average germination day is 1.5 days of cold water 1.5 days of hot water 1.5 days, but the difference between 1.5 days of hot water and 1.5 days of cold water is 0.01 days. The highest germinated uniformity was found in 1.5 days of cold water and 1.5 days of hot water. As a result, in the case of larch and birch, the seed temperature immersion (1.5 days of hot water, 1.5 days of cold water) treatment is superior to the existing three-day immersion treatment in various analysis methods, and it is expected that productivity and efficiency can be improved at a low cost in a short period of time at the seedling production site through seeds.

• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.1
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• pp.36-47
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• 2023

This study was conducted to develop a mass production for a commercial use by germination of 4 kinds of Veronica glabrifolia Kitag., V. pusanensis Y.N.Lee, V. glabrifolia Kitag. × V. Spicata 'Alba' and V. spicata 'Ulster Blue Dwarf × V. longifolia. Veronica L. Effects of dipping time to a disinfecting fluid, light quality, temperature or vermiculite depth on the germination of Veronica L. were examined. Germination percentage in a disinfecting fluid for 120 minutes dipping was the 75.1% for V. pusanensis Y.N.Lee, and was some higher in 60 minutes dipping for all the species, but there was no significant difference according to the dipping time. The germination of the light quality was the best in complex light (red light + blue light) as the germination 92.8%, uniformity 2.3 in Veronica glabrifolia Kitag., or the germination 85.9%, uniformity 3.5 in V. pusanensis Y.N.Lee. And the germination percentage of red light was ranged from 79.1% to 95,5%, the blue light was 83.1% to 100% in the all species. However the germination in the dark condition significantly lowered as a 40.0% to 56.3% or uniformity from 5.2 to 6.5. Like this, the germination of blue light was better compare to the red light. The germination rate according to temperature was higher at 25℃ day/20℃ night ranged from 92.1% to 100%, or at 20℃ day/15℃ night as a 85.5% to 98.9% in the all species, compare to constant temperature 25℃ as a 80.9% in V. pusanensis Y.N.Lee. The germination rate of vermiculite depth was higher in 1 to 2mm ranged from 83.4% to 100%, however when the vermiculite was covered with 4mm, the germination rate significantly decreased from 45.2% to 72.1% and the hypocotyl length became longer above 3cm than that of others.

• Korean Journal of Plant Resources
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• v.19 no.6
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• pp.700-705
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• 2006

Present studies were performed to determine the physiology of seed germination in Viola species native to Korea. Twelve species, 1 variety and 1 form were collected, classified and used as materials: V. collina, V. blandaefomis, V. rosii, V. chaerophylloides, V. phalacrocarpa, V. patrinii, V. mandshurica, V. mandshurica for. albescence, V. seoulensis, V. yedoensis, V. keiskei, V. variegata, V. variegata var. chinensis, and V. verecunda. V. tricolor 'Helen Mount' was also used to compare wild with cultivated species. In order to investigate the effect of temperature on seed germination, seeds stored at $4{\pm}2^{\circ}C$ for 10 months or 4 years were incubated at 10, 15, 20, $25^{\circ}C$ under 16h illumination with 4 replicates per treatment. Seeds which had not germinated at $10^{\circ}C$ were transferred to $30^{\circ}C$ to assess the effect of temperature change in germination. Germination percent and the days of first, 40% and 80% germination were assessed. Capability of seed germination varied with taxon; Species belonging to subsection Patellares had high ability of germination, compared to species in the other subsections, and series Chinensis was the best among subsection Patellares. Species capable of high germination germinated in all temperatures with reasonably high germination rate, but the other species responded sensitively to temperature with different germination patterns. Higher the temperature, shorter the incubation time required for first, 40% and 80% of germination. Therefore, high temperature was effective in almost all species, not only for inducing high rate of germination but also the uniformity of germination. Temperature change from $10^{\circ}C\;to\;30^{\circ}C$ had a positive effect on seed germination.