• Korean Journal of Environmental Agriculture
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• v.17 no.3
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• pp.274-278
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• 1998

To observe the stimulating effects of low-dose ${\gamma}-radiation$ on the Chinese cabbage, $1{\sim}5$ years old seeds were cultivated in the green house and the experimental field after irradiation of $0.5Gy{\sim}30.0Gy$ and the growth characters of them were investigated. The germination rates and the seedling heights of old seeds increased positively in the green house, but different from each other depending on their period of storage. Those of 3-, 4-, and 5-year old seeds increased remarkably at the 4.0Gy-, 1.0Gy-, and 0.5Gy-irradiation group respectively. However, in the case of 1-year and 2-year old seeds, there were not significant effects of irradiation except the increase of seedling height. There were significant increasing effects of radiation on the plant height, fresh weight, and diameter of all seeds grown in the experimental field of 4.0Gy- and 8.0Gy-irradiation group, including the corresponding effects of 1-year and 2-year old seeds in 20.0Gy- and 30.0Gy-irradiation group.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.112-117
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• 2005

This study was conducted to see the feasibility of breeding for sprout soybean cultivar with minimum benny flavor using lipoxygenase-less lines. Lipoxygenase-less cultivar Jinpumkong2 was crossed by lipoxygenase containing Gwangankong, Sebaeknamulkong, and Pureunkong as paternal parent and 24 lipoxygenase-less lines derived from those 3 combinations were selected and those lines were evaluated with their parental cultivars. Germination rate showed no difference between lipoxygenase-less lines and their parental cultivars, however, rates of normal sprout of those and Jinpumkong2 were 63 and $56\%$, and were lower than that of paternal parents. Hypocotyl length of those was same as Jinpumkong2, however, shorter than paternal parents. Texture characteristics including hardness, cutting force and mastication of 96 hour-cultured sprout of lipoxygenase-less lines showed higher value than that of their parental cultivars. Lipoxtgenase isozyme was not detected in the sprout cotyledon of lipoxygenase-less lines, however it was observed in the sprout hypocotyl of all the used genotypes. Though lipoxygenase activity in the seed of lipoxygenase-less lines was lower than that of Jinpumkong2(0.477, ${Delta}A$ 234 nm min-1 mg meal-1),2 lines revealed more than 0.5 value. Lipoxygenase-activity of 2 day-cultured sprout(both cotyledon and hypocotyl) was the highest, decreased in 3 days after culture and re-increased thereafter. Several lipoxygenase-less lines with lower lipoxygenase activity of sprout than Jinpumkong2 were selected and this suggested the possibility of breeding lines for soy-sprout with low benny flavor.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.3
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• pp.201-205
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• 2002

This study was carried out to investigate storability and develop suitable storage method for barley. Rough and milled barley such as Olbori(covered barley), Ssalbori(naked barley) and Chalssalbori (waxy naked barley) were packaged in 3P bag and OPP film bag, respectively, and stored at ambient and low temperature warehouse conditions. The weight loss was higher in milled barley than that of rough barley during barley storage. Acid value, one of the most important factor for grain quality evaluation, was increased from 4.9-9.0 mg KOH/100g to 6.5-19.9mg KOH/100g in milled barley at 16 months after storage. Reducing sugar content was increased while germination and water absorption rates were decreased during barley storage period. In terms of penal taste of boiled barley, rough barley could be stored safely more than one year regardless of storage methods where-ase milled barley stored in ambient temperature ware-house produced nasty flavor at 8 to 10 months after storage.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.71-80
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• 2005

Effects of low temperature ($8^{\circ}C$) on the hydraulic conductivity of young roots of a chilling-sensitive (cucumber; Cucumis sativus L.) and a chilling-resistant (figleaf gourd; Cucurbita ficifolia Bouche) crop have been measured at the levels of whole root systems (root hydraulic conductivity, $Lp_r$) and of individual cortical cells (cell hydraulic conductivity, Lp). In figleaf gourd, there was a reduction only in hydrostatic $Lp_r$ but not in osmotic $Lp_r$ suggesting that the activity of water channels was not much affected by low root temperature (LRT)treatment in this species. Changes in cell Lp in response to chilling and recovery were similar asroot level, although they were more intense at the root level. Roots of figleaf gourd recovered better from LRT treatment than those of cucumber. In figleaf gourd, recovery (both at the root and cell level) often resulted in Lp and $Lp_r$ values which were even bigger than the original, i.e. there was an overshoot in hydraulic conductivity. These effects were larger forosmotic (representing the cell-to-cell passage of water) than for hydrostatic $Lp_r$. After a short term (1 d) exposure to $8\;^{\circ}C$ followed by 1 d at $20\;^{\circ}C$, hydrostatic $Lp_r$ of cucumber nearly recovered and that of figleaf gourd still remained higher due to the overshoot. On the contrary, osmotic $Lp_r$ and cell Lp in both species remained high by a factor of 3 as compared to the control, possibly due to an increased activity of water channels. After pre-conditioning of roots at LRT, increased hydraulic conductivitywas completely inhibited by $HgCl_2$ at both the root and cell levels. Different from figleaf gourd, recovery from chilling was not complete in cucumber after longer exposure to LRT. It is concluded that at LRT, both changes in the activity of aquaporins and alterations of root anatomy determine the water uptake in both species. To better understand the aquaporin function in plants under various stress conditions, we examined the transgenic Arabidopsisand tobacco plants that constitutively overexpress ArabidopsisPIP1;4 or PIP2;5 under various abiotic stress conditions. No significant differences in growth rates were found between the transgenic and wild-type plants under favorable growth conditions. By contrast, overexpression of PIP1;4 or PIP2;5 had a negative effect on seed germination and seedling growth under drought stress, whereas it had a positive effect under cold stress and no effect under salt stress. Measurement of water transport by cell pressure probe revealed that these observed phenotypes under different stress conditions were closely correlated with the ability of water transport by each aquaporin in the transgenic plants. Together, our results demonstrate that PIP-type aquaporins play roles in seed germination, seedling growth, and stress response of Arabidopsis and tobacco plants under various stress conditions, and emphasize the importance of a single aquaporin-mediated water transport in these cellular processes.

• Journal of the Korea Organic Resources Recycling Association
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• v.1 no.2
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• pp.237-258
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• 1993

Objectives in this study are to stabilize the organic sludges which were produced from papermill wastewater, nightsoil treatment facilities and utilized as foods for earthworm, to find the method for using the cast which was orginated by earthworm as fertilizer, and ultimately to develop the practical technique to solve the environmental problems by optimization of wastes treatment. The results of this study were summarized as follows. In the investigation for fertilizer effect of cast, germination rates of cucumber, tomato, and cabbage were investigated. The germination status of 3 types of plants was excellent as over 90% in the combination case of 30-45% cast. In the investigation for fertilizer effect of cast derived from nightsoil sludge, the growing status of cucumber, tomato, and cabbage was excellent in the combination case of 30-45% cast. In the experiment for fertilizer effect investigation of cast derived from papermill sludge, the growing status of cucumber, tomato, and cabbage was excellent in the combination case of 30-45% cast. Also, in the combination case of 60% cast, plants growth was not disturbed.

• Journal of Plant Biotechnology
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• v.40 no.3
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• pp.147-155
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• 2013

In this experiment, we report for the first time mass propagation by in vitro mircrotuberization of Codonopsis lanceolata. We first examined the effect of cytokinins on multiple shoot induction. 2.0 $mg{\cdot}L^{-1}$ of kinetin not only gave the highest rate of shoot induction (19.1%) but also the elongation of shoot (17.1 mm). Secondly, we investigated the effect of sugars on in vitro microtuberization from nodal segments. The diameter of tuberous roots was enlarged in the half-strength MS medium supplemented with 145.9 mM sucrose. Histological analysis revealed that the number of parenchymatous cell containing starch grains increased in the tuberous roots. In addition, unlike in non-tuberous root, vascular bundles were scattered inner cortex layer. Thirdly, in order to preserve and stimulate the germination, microtubers were stored at $4^{\circ}C$ refrigerator during 9 months and then transplanted to the artificial soils (vermiculrite : peatmoss = 1:1 v/v), resulting that the rates of survival and germination were 75% and 70%, respectively. These results indicated that mass propagation of C. lanceolata was achieved by in vitro microtuber formation, suggesting that this protocol might be applied for not only the propagation of elite clones but also conservation of C. lanceolata germplasm.

• Korean Journal of Medicinal Crop Science
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• v.24 no.5
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• pp.360-369
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• 2016

Background: Cylindrocarpon destructans (Zins) Scholten is an important pathogenic fungus that causes ginseng root rot in many ginseng growing areas in China. Although C. destructans have been studied worldwide, research on its chemotaxis towards ginseng (Panax ginseng C. A. Meyer) root exudates in the rhizosphere remains limited. Methods and Results: In this study, we collected ginseng root exudates with three different polarities from three-year-old ginseng roots, and performed chemotaxis and spore germination assays to investigate the ability of these exudates to induce the response in C. destructans. The results showed that, compared with other conditions, when C. destructans cultivated at $20^{\circ}C$ and a pH of 6 exhibited a strong positive chemotactic response toward $2mg/{\ell}$ aqueous phase, $20mg/{\ell}$ butanol phase, and $0.2mg/{\ell}$ petroleum ether from ginseng root exudates, the chemotactic moving indexes were 0.1581, 0.1638 and 0.1441, respectively. In addition, the spore germination rate with optimal chemotactic parameters were 48%, 53%, and 41% in the aqueous phase, butanol phase and petroleum ether groups, respectiviely, which were significantly higher than that in the control group (23%) (p < 0.05). The mycelial growth rate with optimal chemotactic parameters increased with culture time, and the maximum growth rates in the aqueous phase, butanol phase and petroleum ether groups were 0.425, 0.406 and 0.364 respectively, on the 4th day. The optimal chemotactic parameters were $39.73mg/50mg/{\ell}$, $48.93mg/50mg/{\ell}$, and $31.43mg/50mg/{\ell}$, in aqueous phase, butanol phase and petroleum ether respectively, from ginseng root exudates, compared with $5.5mg/50mg/{\ell}$, in the control group. Conclusions: The present study revealed that certain ginseng root exudates containing chemical attractants act as nutritional sources or signals for C. destructans and support its colonization of ginseng roots.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.3
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• pp.1-12
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• 2018

This study was conducted to obtain a reliable result assessing proper amount of solid swine manure for the growth of Deutzia crenata seedlings. Seedling growth dry weight, chlorophyll content inorganic nutrients and soil chemical properties were investigated. 1. When treated with solid swine manure, seed germination rate was highest on the control. However, germination rates tended to decrease when treated with swine manure at high concentrations. 2. The growth of seedlings treated with swine manure was always higher than that of in control. At the 1.0% of swine manure treatment, the growth rate of the seedlings was highest. 3. Seedling dry weight was highest at the 1.0% swine manure treatment. The amount of inorganic nutrients absorbed by the seedling was generally high with the 1.0% treatment, declined sharply with the 2.0% treatment. 4. For the planting soil of Deutzia crenata, the higher the concentration of swine manure, the lower the soil pH. However, nitrogen, available P, K, Na, Mg and Ca contents in the soil have increased with higher concentrations.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.2
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• pp.167-173
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• 1981

Respiration of whole seeds and seed parts isolated from four soybean varieties Essex, Pickett, Wayne and Bonus as measured at $25^{\circ}C$ during germination. The average respiration rates of cotyledons were significantly higher than those of other parts. The values were 35.7, 28.0 and $23.9{\mu}hr^{-1}$ seed$^{-1}$ for cotyledons, embyros, and hila, respectively. Bonus showed a significantly lower respiration rate than the other varieties did. The interaction of variety x seed part was not significant source of variation for respiration. This suggests that seed part and variety independently contribute to soybean seed respiration during early imbibition. The effects of imbibition time and time x variety intereaction on whole seed respiration were significant, but the variety effect was not significant. The correlation coefficient between seed moisture content and respiration after 24 hours of imbibition was significant for Bonus, but not for the other varieties tested. This suggests that Bonus may require more water for respiration during imbibition than the other varieties. There as no significant correlation of whole seed respiration rate after 24 hours imbibition with seed weight or seedling length.

• Korean Journal of Weed Science
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• v.5 no.1
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• pp.56-62
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• 1985

Effects of butachlor [N-(butoxymethyl)-2-chloro-2', 6'-diethyl acetanilide] and 1,8-naphthalic anhydride (NA) on post-germination growth, mesocotyl and root anatomy and root-cell membrane permeability of rice (Orvza saliva L.) were investigated. Lengths of mesocotyl and radicle were markedly decreased as the application rates of butachlor increased from 0.1 to 100 ppmW and NA from 1 to 100 ppmW, but there was no effect on coleoptile elongation. Application of butachlor-NA resulted in increase in coleoptile elongation, but decrease in mesocotyl elongation. Partial breakdown of cortical cells in root and mesocotyl was caused by either trutachlor or NA treatments, resulting in increase in intercellular air space. Further increase in the intercellular air space of root and mesocotyl was obtained when butachlor was applied in combination with NA. Increase in root-cell membrane permeability occurred when either butachlor or NA was applied. However, butachlor-NA treatments resulted in reduction in the permeability.