• Title/Summary/Keyword: germination rates

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Estimation of Cardinal Temperatures for Germination of Seeds from the Common Ice Plant Using Bilinear, Parabolic, and Beta Distribution Models

  • Cha, Mi-Kyung;Park, Kyoung Sub;Cho, Young-Yeol
    • Horticultural Science & Technology
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    • v.34 no.2
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    • pp.236-241
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    • 2016
  • The common ice plant (Mesembryanthemum crystallinum L.) has some medicinal uses and recommended plant in closed-type plant factory. The objective of this study was to estimate the cardinal temperatures for seed germination of the common ice plant using bilinear, parabolic, and beta distribution models. Seeds of the common ice plant were germinated in the dark in a growth chamber at four constant temperatures: 16, 20, 24, and $28^{\circ}C$. For this, four replicates of 100 seeds were placed on two layers of filter paper in a 9-cm petri dish and radicle emergence of 0.1 mm was scored as germination. The times to 50% germination were 4.3, 2.5, 2.0, and 1.8 days at 16, 20, 24, and $28^{\circ}C$, respectively, indicating that the germination of this warm-weather crop increased with temperature. Next, the time course of germination was modeled using a logistic function. For the selection of an accurate model, seeds were germinated in the dark at constant temperatures of 6, 12, 32, and $36^{\circ}C$. Germination started earlier and increased rapidly at temperatures above $20^{\circ}C$. The minimum, optimal, and maximum temperatures were estimated by regression of the inverse of time to 50% germination rate, as a function of the temperature gradient. The different functions estimated differing minimum, optimal and maximum temperatures, with 5.7, 27.7, and $36.5^{\circ}C$, respectively for the bilinear function, 13.4, 25.0, and $36.6^{\circ}C$, respectively, for the parabolic function and 7.8, 25.9, and $36.0^{\circ}C$, respectively, for the beta distribution function. The models estimated that the inverse of time to 50% germination rate was 0 at 6 and $36^{\circ}C$. The observed final germination rates at 12 and $32^{\circ}C$ were 62 and 97%, respectively. Our data show that a beta distribution function provides a useful model for estimating the cardinal temperatures for germination of seed from the common ice plant.

Phytotoxicity and Volatile Monoterpenes of Leaves from Artemisia capillaris and Artemisia iwayomogi Used as Korean Herbal Injin

  • Yun, Kyeong-Won
    • Journal of Ecology and Environment
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    • v.32 no.1
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    • pp.9-12
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    • 2009
  • Artemisia capillaris and Artemisia iwayomogi are weeds used as medicinal plants in Korea under the name "Injin". I collected leaves of A. capillaris and A. iwayomogi, examined them for phytotoxic effects from volatile substances and determined the composition of monoterpenes in the leaves. The effects of volatile substances from each species on seed germination and radicle elongation in each of the two Artemisia species were assessed. The volatile substances of A. capillaris did not negatively affect the seed germination of A. capillaris, but they did inhibit radicle elongation. Rates of seed germination of A. iwayomogi decreased when the seeds were exposed to high concentration of A. capillaris volatile substances. The inhibition of seed germination and radicle elongation by volatile substances from both Artemisia species was stronger for A. iwayomogi than for A. capillaris. I identified the monoterpenoids from the leaves with a gas chromatograph-mass spectrometer (GC-MS). The main constituents of A. capillaris were acenaphthylene (37.91%), $\beta$-pinene (12.08%), 4-carene (10.61%) and $\gamma$-curcumene (9.92%), while those of A. iwayomogi were germacrene-$\delta$ (32.15%), borneol (21.24%), camphor (20.45%) and trans-caryophyllene (7.75%).

Differential antioxidation activities in two alfalfa cultivars under chilling stress

  • Wang, Wen-Bin;Kim, Yun-Hee;Lee, Haeng-Soon;Deng, Xi-Ping;Kwak, Sang-Soo
    • Plant Biotechnology Reports
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    • v.3 no.4
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    • pp.301-307
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    • 2009
  • To understand the adaptability of alfalfa (Medicago sativa L.) to chilling stress, we analyzed the antioxidative mechanism during seed germination. The germination rates of six alfalfa cultivars were studied comparatively at $10^{\circ}C$. Xinmu No. 1 and Northstar were selected as chilling stress-tolerant and stress-sensitive cultivars for further characterization. After chilling treatment, Xinmu No. 1 showed higher seedling growth than Northstar. Xinmu No. 1 exhibited low levels of hydrogen peroxide and lipid peroxidation compared with Northstar. In addition, shoots in Xinmu No. 1 treated with chilling showed higher activities of the superoxide dismutase, ascorbate peroxidase (APX), and catalase than those of Northstar, whereas Xinmu No. 1 showed higher APX activity in roots that Northstar. These results indicated that high antioxidation activity in Xinmu No. 1 under chilling stress is well associated with tolerance to chilling condition during germination.

Effect of Deep Sea Water on Seed Germination, Photoperiod and Temperature on the Growth and Flowering of Buckwheat Species

  • Briatia, Xoxiong;Hong, Soon-Kwan;Sung, In-Je;Chang, Kwang-Jin;Park, Byoung-Jae;Park, Cheol-Ho
    • Korean Journal of Plant Resources
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    • v.25 no.3
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    • pp.323-328
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    • 2012
  • This paper describes the potential use of deep sea water to stimulate seed germination in both common and Tartary buckwheat. Treatment of 10% deep sea water at $25^{\circ}C$ would slightly enhance germination of buckwheat seeds compared to non-DSW treatment and other temperature. In this study, the significant effects of photoperiod and temperature on seedling growth were also found in the HL treatment for the number of leaf, plant height, and plant fresh weight and LL treatment for root length and leaf size. Common buckwheat (Suwon No.1) showed higher rate (93%) of flowering plants in the HS and LL (93% of flowering rates) than those revealed in the HS and LS treatment, while the low percentage(67%) of plant flowering plants was shown in the LS treatment. All plants (100%) of a Korean landrace, Ahndong-jaerae showed flowers in the HS and LS treatment. HL and LL treatment status did not occur in the plant's flowering. Any Tartary buckwheat (KW45) plant did not yet flowered when it was 21 days-old.

Effects of Storage Period and Germination Condition on Germination Rate of Vicia tetrasperma and V. hirsuta (피복식물용 자생잡초 얼치기완두와 새완두 종자의 저장방법이 발아에 미치는 영향)

  • Cho, Jung-Lai;Choi, Hyun-Sug;Lee, Youn;Kim, Chang-Seok;Lee, In-Yong
    • Korean Journal of Weed Science
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    • v.31 no.1
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    • pp.78-83
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    • 2011
  • This study was conducted to evaluate germination rate of Vicia tetrasperma and V. hirsuta as affected by storage duration, temperature, and light. All seeds of both species were gathered from Dangjin, Chungnam in the late June of 2004 and 2005, stored for a certain period of time, and then exposed for 20 days to the alternating temperatures ($15/^{\circ}C,\;20/10^{\circ}C,\;25/15^{\circ}C$, and $30/20^{\circ}C$) for evaluating the germination rate. Both 2004 and 2005, two species stored at room temperature for 3 months had germination rates less then 30% with applying various alternating temperatures for 20 days although the highest germination rate was obtained with $25/15^{\circ}C$ treatment. V. tetrasperma at 12 month storage after seed gathering in 2005 had 82% of germination rate with $25/15^{\circ}C$ treatment and showed the increased rate as time passed. V. hirsuta at 6, 9, and 12 month storage after seed gathering in 2005 ranged, however, from 33 to 53% of the germination rate at alternating temperature treatments for 20 days and did not seem to be affected by the elapsed time of the storage. Storage at room temperature was more effective for increasing germination rate of both species compared to those of the storage at low temperature. The germination rate of both species was not significantly affected by either exposure of light or short period of high temperature.

Effects of mechanical Injury, cotyledon elimination and nutrition for the germination of apple seeds (Malus pulmila var. domestica) (사과 종자 발아에 미치는 기계적 상처, 자엽제거 및 영양분의 영향)

  • Choi, Pil Son;Lee, Young Jin;Yu, Young Beob;Choi, Myung Suk
    • Journal of Plant Biotechnology
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    • v.46 no.4
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    • pp.318-322
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    • 2019
  • When dehulled apple seeds (Malus pilmila var. domestica) were induced to germinate, the rate of germination, which was increased by piercing both the radicle and cotyledon, was related to the site of injury. Also, the germination rates of an embryo with ¾ cotyledon (¾ CE), embryo with ½ cotyledon (½ CE), embryo with ¼ cotyledon (¼ CE), and embryo without cotyledon (NCE) gradually decreased compared to an embryo with a normal cotyledon, but it significantly recovered on the agar media containing MS solution and/or 80 mg/L gibberellin (GA3) as nutrition. The results show that the germination of the embryo was inhibited by the partial removal of cotyledon and also demonstrated that the rate of germination could be significantly increased by adding GA3 (80 mg/L) and/or MS medium.

Effective Screening Condition for Low-Temperature Germinability of Rice (벼 저온발아성의 효율적 검정 조건)

  • Kang, Jong-Re;Lim, Sang-Jong;Kim, Soon-Chul;Ko, Mi-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.6
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    • pp.711-715
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    • 1995
  • This study was carried out to determine the desirable screeing condition for low-temperature germinability of rice seeds. The germination test was performed with fifty rice varieties from three countries, mainly including the recent Korean rice cultivars. The analyzed result of germination test under three temperature conditions(13$^{\circ}C$, 16$^{\circ}C$, $25^{\circ}C$) pointed out that 11 days after seeding at 13$^{\circ}C$ was the most efficient testing condition for low-temperature germinability in terms of mean and variation in germination rate. Highly significant correlations were observed between germination rates of 11 days after seeding at 13$^{\circ}C$ and germination coefficients, average days of germination at 13 for 20 days. Low-temperature germinability were highly positively associated with days to heading or amylose content, but negatively correlated with grain width.

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Effect of Sodiun Hypochlorite Pretreatment, Light Intensity and Depth of Soil Covering on Germination of Cattail(Typha spp.) Seeds (Sodium Hypochlorite 처리와 광도 및 복토 깊이의 차이가 부들의 종자 발아에 미치는 영향)

  • Kim Young-Ju;Heo Jin-Ah;Hwang Yong-Soo;Ku Ja-Hyeong
    • Asian Journal of Turfgrass Science
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    • v.19 no.2
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    • pp.115-123
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    • 2005
  • The effect of sodium hypochlorite treatment on the germination of cattail (Typha spp.) seeds was investigated in growth chambers maintained on a 14-h photoperiod with various temperatures and light intensities. Germination rates of seeds were, in general, enhanced by the increase of light intensity and temperature regardless of cattail species. Seeds of T. oreientalis had 4.3, 13.0 and $7.3\%$ germination at temperatures of 20, 25 and $30^{circ}$C, respectively, under light intensity of 7.5${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. T. angustata showed higher germination rate, thus, 10.7, 22.7 and $50.7\%$ under same temperature and light environment. Under high light intensity of 79.5${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, the germination rates of T. oreintalis and T angustata were $78.3\%$ and $88.7\%$ at $30^{circ}$C, respectively. Scarification of seeds with NaOC1 ($4\%$, available chlorine) increased germination rate in both species, especially even at low temperature of $20^{circ}$C. Germination speed was also enhanced by NaOC1 treatment. High light intensity further increased the germination rate. When NaOC1 treated seeds were sowed on the soil surface in plastic house, the seedling emergence was nearly $100\%$. Untreated seeds of T. oreintalis and T. angustara showed 40 and $50\%$, respectively, in germination under same condition. However, when the depth of soil covering was over 1.0 cm, seedling emergence was retarded more than 1 month. On the process of seedling development, emergence of mesocoty1 occurred firstly and after than primary root and first leaf were developed on the end of elongated mesocotyl. These results suggest that the promotion of seed germination by NaOC1 pretreament may be induced from the increase of light absorptivity as well as water permeability through scarifying and bleaching the seed coat.

Seed Treatment and Plug Production of Ornamental Grasses (Gramineae spp.) (화본과 관상용 그래스의 종자처리와 플러그 묘 생산)

  • Kim, Ki Dong;Kim, Yoon Joong;Lee, Jong Min;Lee, Jeong Ho;Joo, Young Kyoo
    • Weed & Turfgrass Science
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    • v.2 no.4
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    • pp.376-380
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    • 2013
  • This research was carried out to establish a mass-production method and to develop ornamental Gramineae species as cash crops for famers as three species of ornamental grasses were used for seed treatment and plug production. Germination rates of Arundinella hirta were improved to 52.6% by soaking in 4% NaOCl for 60 min and 49.4% with 4% NaOH for 30 min. Germination rates of Panicum virgatum were 20.3% and 22.4% when treated with 4% NaOCl for 10 min and 2% NaOH for 60min treatment, respectively. Pennisetum alopecuroides showed germination rates higher than 98% with both 4% of NaOCl and NaOH for 60 min. When the same size of trays were used, Pennisetum showed faster plug formation in 21 and 30 days with 162 and 40 cell trays, respectively. Arundinella showed a similar trend with Pannicum in completing plug formation, those took 32, 41, and 44 days with the 162, 72, and 40 cell tray, respectively. Plug production was faster with smaller cells; however, total growing period was longer with larger cells in general.

Development of Restoration Technology Using Native Plants Seed Chip for Various Disturbed Slopes (자생식물 종자칩(seed chip)을 이용한 훼손사면의 복원 공법의 개발)

  • Suh, Hyoung-Min;Kim, Dong-Geun;Lee, Nam-Sook
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.13 no.1
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    • pp.112-120
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    • 2010
  • This study was carried out to develop the environmental friendly restoration technique for various disturbed slopes using native plants seed chips. As the result of the separated seeding using the developed seed chips of native plant combination, average germination rate and survival rate of trees, shrubs, and herbs were higher than those of the direct seeding, as 79~70%, 83~73%, and 59~53% or so, respectively. Also, the germination rates and the survival rates of trees, shrubs, and herbs in the separated seeding by the native plants seed chips were 10~15 times higher than those of the direct seeding. Numbers of plants survived were at least 10 times of that of the simple seeding. The native plants seed chip technique is more efficient in soil slopes compared with stone slopes. The application of the native plants seed chip technique makes the restoration stabilized through the herbs dominant vegetation to the woody dominant 1 within 3 years, and the Landscapes also changed from herbs to variously combined herbs and woody. The technique using native plants seed chips that can be applicable to various disturbed slops is more efficient than conventional restoration techniques in the respect of economics and landscape.