• Title/Summary/Keyword: germination inhibition

Search Result 287, Processing Time 0.025 seconds

Some Physiological Activities Observed from Ethanol Extracts of Germinated Specialty Rough Rice

  • Lee, Youn-Ri;Hwang, In-Guk;Woo, Koan-Sik;Kim, Kee-Jong;Jeong, Heon-Sang
    • Preventive Nutrition and Food Science
    • /
    • v.13 no.3
    • /
    • pp.176-181
    • /
    • 2008
  • This study analyzed the some of the physiological activities of 70% ethanol extracts of specialty rough rice ('Goami2', 'Keunnunbyeo', and 'Heugkwangbyeo' rough rice cultivars) before and after germination. The physiological activities of the extracts were analyzed for their inhibition of linoleic acid peroxidation, hydrogen peroxide radical scavenging activity, superoxide dismutase-like activity, xanthine oxidase inhibitory activity, and nitrite scavenging effects. The most active rough rice cultivar was 'Heugkwangbyeo' and germinated 'Heugkwangbyeo' rough rice was more effective than ungerminated rough rice, having higher inhibition activity of linoleic acid peroxidation (1.2-fold), hydrogen peroxide radical scavenging activity (1.4-fold), superoxide dismutase-like activity (1.2-fold), and xanthine oxidase inhibitory activity (1.1-fold). The nitrite scavenging effect was increased after germination (2.1-fold) and decreased as pH increased. According to the results obtained in this study, 70% ethanol extracts from germinated 'Heugkwangbyeo' rough rice can be used for industrial applications such as the functional food materials.

Effects of Humic Acids on Growth of Herbaceous Plants in Soil Contaminated with High Concentration of Petroleum Hydrocarbons and Heavy Metals (고농도 유류와 중금속으로 복합 오염된 토양에서 식물성장에 미치는 부식산의 영향)

  • Kim, Ki-Seob;Sung, Ki-June
    • Journal of Soil and Groundwater Environment
    • /
    • v.16 no.1
    • /
    • pp.51-61
    • /
    • 2011
  • Germination tests were conducted to determine the practical concentration levels at which plants can reproduce naturally during the phytoremediation of soils contaminated with a high concentration of petroleum hydrocarbons and heavy metals. The effects of humic acids on plant growth and soil physicochemical properties were also investigated. The results show that phytoremediation can be applied in soils contaminated by multiple contaminants at the former soil contamination potential level of Korean soil quality standards considering successful natural reproduction. It was observed that germination rates of Helianthus annuus and Festuca arundinacea were high after all treatments, and transplantation was more appropriate for Phragmites communis in phytoremediation. Humic acids had a positive effect on the growth of both aboveground and belowground biomass of herbaceous plants. Growth inhibition by multiple contaminants is more severe in the case of aboveground biomass. Germination and growth tests suggest that Helianthus annuus is a suitable phytoremediation plant for soils contaminated with a high concentration of petroleum hydrocarbons and heavy metals. The addition of humic acids also caused changes in the physicochemical properties of contaminated soils. An increase in the carbon and nitrogen content due to the addition of humic acids and a correlation between cation exchange capacity(CEC) and the organic matter content were observed.

Quantifying Inhibitory Effects of Reclaimed Soils on the Shoot and Root Growth of Legume plant Lentil(Lens culinaris) (정화 처리토가 렌틸(콩과식물)의 지상부 및 뿌리 성장에 주는 영향에 대한 정량평가)

  • Park, Hyesun;Kang, Sua;Bae, Bumhan
    • Journal of Soil and Groundwater Environment
    • /
    • v.26 no.5
    • /
    • pp.1-8
    • /
    • 2021
  • A series of pot experiments were conducted to quantitatively estimate inhibitory effects of reclaimed soil on the growth of Lentil (Lens culinaris) with two soils remediated by land farming (DDC) and low temperature thermal desorption(YJ), respectively. After cultivation in a growth chamber for 8 days, plants were harvested for the analysis of 8 indices including chlorophyll-a and carotenoid in leaves, shoot fresh weight, root dry weight, root length, number of later roots, specific root length (SRL) as well as germination rate in comparison to control experiment conducted on nursery soil. Root length was estimated by SmartRoot program from the digital images of the roots. The results showed germination rate on YJ and DDC soil decreased 29 and 71%, respectively. In comparison to the control, the averaged value of the 8 indices for YJ and DDC soil showed overall growth inhibition was 48 and 68%, respectively. When the same experiment was conducted with 25% (W/W) vermiculate amended soil, plant growth on each soil was comparable to that of the control. The results implies reclaimed soils requires additional processes and/or amendments to reuse for plant growth.

In vitro Anti-fungal Activity of Various Hydroxylated Fatty Acids Bioconverted by Pseudomonas aeruginosa PR3

  • Bajpai Vivek K.;Kim, Hak-Ryul;Kang, Sun-Chul
    • Journal of Applied Biological Chemistry
    • /
    • v.49 no.4
    • /
    • pp.131-134
    • /
    • 2006
  • The in vitro anti-fungal activity of hydroxylated fatty acids obtained from microbial conversion by Psuedomonas aeruginosa PR3 using ricinoleic acid(RA), eicosadienoic acid(EDA) and conjugated linoleic acid(CLA) as substrates, was investigated. Bioconverted hydroxylated fatty acids showed different anti-fungal activities potentials against the range of phytopathogenic fungi such as Botrytis cinerea, Rhizoctonia solani, Fusarium oxysporum, Sclerotonia sclerotiorum, Colletotricum capsici, Fusarium solani and Phytophthora capsici. RA and EDA showed up to 50% fungal mycelial inhibition at the concentration of $5{\mu}l\;ml^{-1}$. RA, EDA and CLA also exhibited anti-fungal activities with minimum inhibitory concentration(MIC), ranging from 500 to $1000{\mu}g\;ml^{-1}$. Screening was also carried out using varied concentrations of bioconverted RA and EDA for determining the anti-fungal effect on the spore germination of different fungi. Bioconverted RA and EDA showed a considerable degree of spore germination inhibition.

Allelopathic Effect of Pinus rigida Mill (리기다소나무의 Allelopathy 효과)

  • Kil, Bong-Seop
    • The Korean Journal of Ecology
    • /
    • v.11 no.2
    • /
    • pp.65-76
    • /
    • 1988
  • A number of laboratory and field experiments have performed with aqueous extracts from fresh leaves, fallen leaves and roots of Pinus rigida in order to investigae the effetct of the pine tree. The results obtained found its inhibitive effect on the germination and growth of tested species. Though relative germination ratio of the species was somewhat lower than the control plot, severe inhibition was observed in relative dry weight ration, These results were more obvious in aqueous extracts than in the case of leachates and similar results were also confirmed by field bioassays on the forest soil of P. rigida. Therefore, to find out the inhibitory sybstancesm 14 different kinds of KDICical substances were isolatd from the leaved of the tree by chromato-graphy and nist if them were confirmed to be phenoic compounds, The growth of lettuce was inhibition was observed in the concentration of less than 5X10-3M soluton. Phenolic compounds are assumed the substance related to the allelopathic effect of P. rigida.

  • PDF

Effect of Germinated Seed of Rhynchosia Volubilis on Interleukin-6 in Ovariectomized Rats (발아(發芽)한 서목태(鼠目太)가 난소적출 흰쥐의 Interleukin-6에 미치는 영향)

  • Jung Ho-Long;Cha Yun-Yeou
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.19 no.5
    • /
    • pp.1261-1266
    • /
    • 2005
  • The aim of this study is to find out what inhibition effects the Germinated Seed of Rhynchosia Volubilis(GSRV) has by its feeding period on the bone absorption in ovariectomized white rats. Close examination of the amounts of the Interleukin-6 in the rats by the groups of one, two and three-day germination periods led to the following conclusions. Feeding ovariectomized white rats with GSRV by the groups of one, two and three-day germination periods showed less amounts of Interleukin-6 in their bone marrow in all the feeding periods of two, three and four weeks than in the case of control group, revealing a statistical significance. Therefore, the Germinated Seed of Rhynchosia Volubilis(GSRV) is believed to have inhibition effects on bone resorption.

A Survey of Old-field Herbs for Susceptibility to Phenolic Compounds (페놀화합물에 대한 묵밭 초본식물의 감수성)

  • Stowe, L. Gordon;Kil, Bong-Seop;Yim, Yang-Jai
    • Journal of Plant Biology
    • /
    • v.30 no.1
    • /
    • pp.11-20
    • /
    • 1987
  • Phenolic compounds, p-coumaric and p-hydroxybenzoic acids, known as inhibitors for development and growth of many pioneer species on early stage of succession were used for the test fo susceptibility in various herbs collected from abandoned agricultural fields in the vicinity of Amherst(U. S. A.). The percent inhibition was generally greater for p-coumaric acid than for p-hydroxybenzoic acid. Concentrations of 5$\times$10-5 and 5$\times$10-4M generally had no significant effects, but at 5$\times$10-3M was inhibitory to germination and growth of tested species. And the percent inhibition caused by the two phenolics was correlated (r=.843, p<.01). Also the indices of resistance for germination and elongation were significant (r=.695, p<.01) in this study. While Cirsium and Lepidium invading species of early stage of succession were sharply susceptible for toxic activity by phenolic acids.

  • PDF

Effect of Pre-sowing Treatments on Seed Germination and Seedling Growth of Canarium resiniferum, A Rare Native Tree of Bangladesh

  • Hasnat, G.N. Tanjina;Hossain, Mohammed Kamal;Alam, Mohammed Shafiul;Hossain, Md. Akhter
    • Journal of Forest and Environmental Science
    • /
    • v.33 no.3
    • /
    • pp.226-232
    • /
    • 2017
  • Dhup (Canarium resiniferum) is an economically and aesthetically important rare native tree species of Bangladesh. In natural condition 78.5-98.7% seeds do not germinate due to inhibition by hard seed coat, seed predation and unfavorable environmental conditions. A study was conducted in the Seed Research Laboratory and nursery of Institute of Forestry and Environmental Sciences, Chittagong University, Bangladesh in 2013 to find out appropriate pre-sowing treatments for maximizing germination and initial seedling growth. Eleven pre-sowing treatments were provided in both the seeds sown in polybags and seeds sown in propagator house. Results revealed that, germination started at first (after 20 days of seed sown) in seeds immersed in water at room temperature for 24 hours and germination completed within 38 days. Significantly higher (p<0.05) germination percentage (33%), germination energy (16.7%), plant percent (33%) and germination value (0.4) was found with seeds immersed in water at room temperature for 24 hours. Seedling height measured at three, four and five months after the seed germination in case of seeds treated by immersion in water for 24 hours was also greater than others. Therefore, pre-sowing treatment by immersion in water at room temperature for 24 hours was more effective in germination and production of quality vigor seedlings of Dhup.

Chemical Responses and Fitness-Related Characteristics of Dichlofluanid-Resistant and -Sensitive Botrytis cinerea Isolates (Dichlofluanid 저항성 및 감수성 잿빛곰팡이병균(Botryits cinerea)의 약제 반응과 생태 적응력과 관련된 특성)

  • 임태헌;김병섭;조광연;차병진
    • Korean Journal Plant Pathology
    • /
    • v.11 no.3
    • /
    • pp.245-251
    • /
    • 1995
  • Nine hundred and ninety-two isolates of Botrytis cinerea were obtained from infected strawberries, tomatoes and cucumbers in Taejon, Gongju, Puyo, Nonsan and Kimhae in Korea. Six hundred forty-two (64.7%) isolates were benomyl resistant (BR), 245 (24.7%) were procymidone resistant (PR), and 105 (10.6%) were dichlofluanid resistant (DR). In the minimum inhibitory concentration (MIC) test, DR isolates showed mycelial growth on the PDA incorporated with 100 or 500 $\mu\textrm{g}$/ml of dichlofluanid, while dichlofluanid sensitive (DS) isolates did not grow on the PDA incorporated even with 10 $\mu\textrm{g}$/ml of dichlofluanid. Chemical concentrations for inhibition of spore germination were much lower than those for inhibition of mycelial growth. IC50 values, the effective concentrations for 50% inhibition of spore germination, for DR were 0.11~0.29 $\mu\textrm{g}$/ml, whereas they were 0.04~0.09 $\mu\textrm{g}$/ml for DS isolates. In comparison of fitness-related characteristics such as virulence, sclerotial formation, and sporulation, DR isolates were inferior to DS isolates. However, mycelial growth was little different between DR isolates and DS isolates.

  • PDF

Assessment of Allelopathic Potential of Some Weed Species on Alfalfa(Medicago sativa L.) Germination and Early Seedling Growth (알팔파 발아와 초기생육에 대한 잡초종의 Allelopathic 잠재성 평가)

  • Chung, I.M.;Miller, D.A.
    • Korean Journal of Weed Science
    • /
    • v.15 no.2
    • /
    • pp.121-130
    • /
    • 1995
  • Greenhouse and laboratory studies were conducted to investigate allelopathic potential of some weed species on alfalfa(Medicago sativa L.) germination and seedling growth. In the comparison between top(leaves+stems) and root extracts, top extract exhibited greater allelopathic effects on alfalfa germination than that of root. The various weed species extract differently responded to alfalfa test species, WL-320, in terms of allelopathic effect. Top and root aqueous extracts of lambsquarter(Chenopodium album L.), giant foxtail(Setaria faberii Herrm.), redroot pigweed (Amaranthus retroflexus L.), velvetleaf(Abutilon theophrasti Medic.), crabgrass(Digitaria sanguinalis L.), canada thistle(Cirsium arvense L.) and prostrate knotweed(Polygonium aviculare L.) significantly inhibited germination, seedling length, weight, vigor, and rate of germination of alfalfa. The regression slopes of various top extracts showed that velvetleaf(b=3.69) extracts were the most inhibitory, while large crabgrass(b=2.39) extracts had the least allelopathic effect on alfalfa germination. Germination, seedling length and weight of alfalfa were inversely proportional to the concentration of dried velvetleaf extracts. Also, more of the toxic effects were observed from the dried extracts compared to the fresh extracts. Residue of velvetleaf inhibited significantly alfalfa emergence and survival percentage compared to the control. The emergence and survival percentage of alfalfa were 44%, 57% at 1.0% residue treatment, respectively. When weed residues were mixed with silica sand with incubation time, velvetleaf residue most inhibited alfalfa growth. The degree of inhibition increased as incubation time increased. An incubation for 72h caused the greatest inhibition of alfalfa growth. These results demonstrate the different allelopathic activity of weed species extracts on alfalfa and suggest that weed may affect alfalfa growth and development through the inhibitory effects of allelochemicals present in weed tissue.

  • PDF