• Archives of Plastic Surgery
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• v.33 no.4
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• pp.495-498
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• 2006

Purpose: Trichoblastic fibroma originates from hair germ layer tumor which is a benign tumor mixture of epidermal and mesodermal factor. Trichoblastic fibroma was found only in adults and showed equal occurrence rate between men and women. Since it is a rare tumor, we report a case of a trichoblastic fibroma which developed on the right cheek. Methods: A 72 year-old male was treated with excisional operation 17 years ago due to a solitary tumor that developed on the same site. He returned to the hospital with an asymptomatic mass which have been increasing in size for the last 3 months. Results: In computerized tomography, a size of $2.7{\times}2.3{\times}0.8cm$ tumor was found in the subcutaneous tissue layer. Grossly, the mass was well-circumscribed, smooth-surfaced and flesh colored, and was lobulated and fragile. Pathologic observation showed diverse shaped and sized tumor cell nests and fibrocellular stroma consisting basophilic cells in dermal and subdermal layers. Immunohistopathologic staining showed positive reaction on pancytokeratin, CK-5/6, and bcl-2. Conclusion: By having no connection to the epidermis, and being positioned in the dermal and epidermal layers, typical pathologic findings make it possible to differentiate this tumor with basal cell carcinoma. This lesion is not clear whether it is a local recurrence or not, and it is necessary to observe a new recurrence in the future.

• Advances in pediatric surgery
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• v.9 no.2
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• pp.94-97
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• 2003

The teratoma is a unique complex neoplasm and is one of the most frequent pediatric tumors originated from the extragonadal germ cells. Mature teratoma is composed of mature differentiated tissues, while immature teratoma always contains embryonic tissues of variable degrees of immaturity, especially in the neuroepithelial elements. Diagnosis of teratoma is relatively easy by conventional radiologic study, but the immaturity can be identified only by histopathological examination. Between January 1993 to December 2002, 63 cases of teratoma were operated and analysed retrospectively at the Chonnam University Hospital Female to male ratio was about 3:1 and age distribution was relatively even. Among 63 cases, gonadal teratoma was the most common (52.4%), followed by sacrococcygeal (25.4%), retroperitoneal (9.5%) and mediastinal teratoma (9.5%). Fifty-six cases were mature teratomas and seven were immature teratomas. Alpha-fetoprotein (AFP) was elevated in 4 of 6 immature cases, but in 2 of 51 mature ones Elevated AFP progressively returned to normal range by 1 month after operation in all. Complete excision of the mass was performed, and major complication was not noticed. In five immature cases, PEB chemotherapy (Cisplatin, Etoposide, Bleomycin) was performed. Two of 2 cases in histological grade II were well tolerated to the aggressive chemotherapy. One of three cases in grade III expired due to severe bone marrow depression, and two of them expired by tumor recurrences. In conclusion, immature teratoma in histological grade III showed high potentiality of recurrence. Therefore, postoperative chemotherapy has to be applied to the high graded immature tumors.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.3
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• pp.219-224
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• 2000

Reproductive biology of the naked-headed goby, Faronigobius gymnauchen was investigated by means of histological methods. The ovary was consisted of several ovarian lamellae and the oogonia originated from the inner surface of the ovarian lamella. The testis was seminiferous tubule One in internal structure. Seminiferous tubule was consisted of many testicular cysts which contained numerous germ cells in a same developmental stage. The size of group maturity was 4.5 cm intotal length. Gonadosomatic index(GSI) of the female and male was the highest in June and July, respectively. Reproductive cycle could be classified into the growing ($January{\~}March$), maturation ($April{\~}May$), ripe and spent (June{\~}July$), and recovery and resting ($August{\~}December$). Oocyte development was group-synchronous, and yolk nucleus was observed in the early growing oocyte.

• Fisheries and Aquatic Sciences
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• v.19 no.1
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• pp.5.1-5.7
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• 2016

The study was conducted to know the cyclic changes in gonadal maturation and to investigate the developmental stages of oocytes and testicular germ cells of an air-breathing fish, Channa striata. Fish were sampled monthly from lentic and lotic environments of three geographical locations of Bangladesh from December to November and the histological analysis of their gonad was done to evaluate the objectives. The highest mean GSI was $5.95{\pm}0.20$ for female in July and $0.14{\pm}0.01$ for male also in July showing that the gonadal development reached its peak during this month. The highest mean oocyte diameter was $1257.50{\pm}24.17{\mu}m$ observed in July implying that the oocyte reached maturity in this month. Histological study of ovary revealed the evidence of early yolk granule stage and late yolk granule stage from April to July. In case of male four stages of spermatogenesis were distinguished and spermatozoa were highly abundant in June and July. So the monthly pooled values of GSI and the analysis of gonadal histology indicated that the peak breeding season of C. striata occurred in July in the lentic and lotic environments. Samples collected from lentic and lotic habitats are suggestive of no difference in the development of the gonad. The results of the present study will be useful for selective breeding programme, conservation and sustainable fishery management of C. striata in its natural habitat.

• Korean Journal of Animal Reproduction
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• v.22 no.1
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• pp.19-27
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• 1998

The present study was performed to investigate the patterns of a, pp.arance of lectin in uterus of fetuses of 90 and 120 days old and neonates of Korean native goat. The carbohydrate markers were used in histochemistry for the determination of the lectin by staining of avidin-biotin-per-oxidase complex(ABC), rincinus communis agglutinin(RCA-I), ulexeuropalus communis agglutinin(UEA) and wheat germ agglutinin(WGA). 1. The effects of this study were as follows; 1. The binding reactions for Con-A were weak on the mucosal epithelium of endometrium in 90 and 120 days old fetuses, and neonates and moderate at the free surface of mucosal epithelia. 2. The binding reactions for DBA was partially moderate on the mucosal epithelium of endometrium and partially strong at the free surface of mucosal epithelia in 120 days old fetuses. In neonates, the reactions were strong on the mucosal epithelium and gland primordium of endometrium, and the secretions at the free surface showed strong reactions for DBA. But, in 90 days old fetuses, the reaction was negative. 3. The binding reactions for RCA-I were moderate on the mucosal epithelium of endometrium and at the free surface of mucosal epithelia in 90 days old fetuses. In 120 days old fetuses, the reactions were weak on the mucosal epithelium of endometrium and moderate at the free surface of mucosal epithelia. In neonates, the reactions were moderate on the mucosal epithelium of endometrium and strong at the free surface of mucosal epithelia and also strong in the uterine gland. 4. The binding reactions for UEA were negative in 90 and 120 days old fetuses and neonates. 5. In 90 days old fetuses, the binding reactions for WGA were generally weak on the mucosal epithelium of endometrium, but several epithelial cells showed moderate reaction for WGA. In 120 days old fetuses and neonates, the reactions were moderate on the mucosal epithelium and blood vessels of endometrium and strong at the free surface of mucosal epithelia.

• Korean Journal of Environmental Biology
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• v.32 no.2
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• pp.153-158
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• 2014

In order to determine the testicular cycle of the Korean brown frog, Rana coreana, the gonadosomatic index (GSI) and the changes of germ cells in testis for adult males were investigated throughout the year. The study indicated that the spermatogenesis in the seminiferous tubule of testis began in August and became most active in the month of September, and the GSI was recorded the highest and the cross area of seminiferous tubule was the widest on this period. Furthermore the seminiferous tubules at the post spawning stage appeared in testis during February, and the spermatogenesis was quiescence period of time from March to July and the GSI and the cross area of seminiferous tubule were found to be the lowest. Based on these observations, we suggest that, GSI of male Korean brown frog changes significantly between July to August, indicating the testicular cycle with discontinuous spermatogenic process, and the breeding season was confirmed to be February.

• Journal of Preventive Medicine and Public Health
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• v.25 no.1 s.37
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• pp.64-72
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• 1992

To provide the information necessary for the insurance medicine management plan, price discount rates among the insurance medicines were studied. A total of 2,107 items of insurance medicine of which prices were discounted via governmental inspections of real transactional process of insurance medicine were analysed. The conclusions are as follows; 1. Among the variables relevant to the characteristics of manufacturers, price discount rates of insurance medicines were statistically significant with production rankings of manufacturers, incorporation year, existence of investments by foreign corporation, existence of a research institute, and enrollment in the exchange. And among the variables relevant to the properties of medicines, the number of enrolled items which have the same components, classification, the date of new enrollment, the sales of items, and the number of raw materials in the items were statistically significant. 2. Stepwise multiple regression was done to identify the factors which affect the price discount rates of insurance medicines. The number of enrolled items which have the same components, production rankings of manufactures, classification number (medicines for function of tissue cells), incorporation year(1940-1949), existence of investments by foreign corporations, classification number (anti-germ medicines), number of raw materials In the items, the sales of items, and medicines whose major objective is not treatment were significant variables and the $R^2$-value for these variables was 21.2%. Considering all of the above results, for management of insurance medicines, it seems important that the real transactional prices of insurance medicines should be identified systematically, focusing on the properties which affect the price discount rates of insurance medicines.

• The Korean Journal of Zoology
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• v.33 no.3
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• pp.365-372
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• 1990

For the effort to produce transgenic amphibians, a plasmid DNA sequence (cytoplasmic actin promoter-linked bacterial $\beta$-galactosidase gene) was microinjected into fertilized Xenopus eggs. It appeared that the injection of 20 nl solution containing 1-2 ng of DNA was not toxic, but over 4 ng was toxic to embryonic development. The translational product of $\beta$-gal gene ($\beta$-galactosidase) had enzyme activity in all three germ layers of the embryo. Expression of the injected $\beta$-gal genes was first detected at mid-gastrula stage, and the activity persisted up to stage 43 (feeding tadpole) with decreased level of retention. However, the level of the expression was various among the injected individuals as well as each experiment. That is, $\beta$-galactosidase activities did not appear in all cells, instead a localized distribution pattern. Although other possibilities could not be omitted, this mosaic distribution of gene expression seemed to arise from unequal partition of the injected DNA into each blastomere during early cleavage.

• Korean Journal of Environment and Ecology
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• v.29 no.4
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• pp.525-532
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• 2015

In order to determine the testicular cycle of the Asian toad, Bufo gargarizans, adult males of the species were captured around Jeongeup city (Jeollabuk-do, Korea) during March, 2012 to February, 2013 and the gonadosomatic index (GSI) and the changes of germ cells in their testes were investigated throughout the year. The study indicated that the spermatogenesis in the seminiferous tubule of testes began in April and became most active in July. The recorded GSI was the highest and the cross area of seminiferous tubule was the widest in this period. The seminiferous tubules at the post spawning stage appeared in February, the largest amounts occurred in March and primary spermatogonia also appeared in this period. The GSI and the cross area of seminiferous tubules were found to be the lowest in March, indicating a testicular cycle with potentially continuous spermatogenic process. According to the findings above, it is confirmed that testicular spermatogenesis takes place actively between April to July in male Asian toad and that their breeding season is February to March.

• The Korean Journal of Mycology
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• v.21 no.2
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• pp.120-126
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• 1993

Morphological characteristics in the anamorphic states were described in three species of powdery mildew fungi, Microsphaera akebiae, M. picrasmae and M. schizandrae, which were parasitic on Akebia quinata, Picrasma quassioides, and Schizandra chinensis, respectively. All species were flexuous in foot-cells of conidiophores, producing conidia singly on conidiophores, devoid of fibrosin bodies in conidia, and producing germ tubes on the end of conidia.