• Journal of Plant Biotechnology
• /
• 제38권2호
• /
• pp.117-124
• /
• 2011

Considering that the world population is expected to total 9 billion by 2050, it will clearly be necessary to sustain and even accelerate the rate of improvement in crop productivity. In the 21st century, we now face another, perhaps more devastating, environmental threat, namely climate change, which could cause irreversible damage to agricultural ecosystem and loss of production potential. Enhancing intrinsic yield, plant abiotic stress tolerance, and pest and pathogen resistance through agricultural biotechnology will be a critical part of feeding, clothing, and providing energy for the human population, and overcoming climate change. Development and commercialization of genetically engineered crops have significantly contributed to increase of crop yield and farmer's income, decrease of environmental impact associated with herbicide and insecticide, and to reduction of greenhouse gas emissions from this cropping area. Advances in plant genomics, proteomics and system biology have offered an unprecedented opportunities to identify genes, pathways and networks that control agricultural important traits. Because such advances will provide further details and complete understanding of interaction of plant systems and environmental variables, biotechnology is likely to be the most prominent part of the next generation of successful agricultural industry. In this article, we review the prospects for modification of agricultural target traits by genetic engineering, including enhancement of photosynthesis, abiotic stress tolerance, and pest and pathogen resistance associated with such opportunities and challenges under climate change.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권8호
• /
• pp.1150-1159
• /
• 2018

Objective: Non-synonymous single nucleotide polymorphisms (nsSNPs) were identified in Berkshire selective sweep regions and then were investigated to discover genetic nsSNP mechanisms that were potentially associated with Berkshire domestication and meat quality. We further used bioinformatics tools to predict damaging amino-acid substitutions in Berkshire-related nsSNPs. Methods: nsSNPs were examined in whole genome resequencing data of 110 pigs, including 14 Berkshire pigs, generated using the Illumina Hiseq2000 platform to identify variations that might affect meat quality in Berkshire pigs. Results: Total 65,550 nsSNPs were identified in the mapped regions; among these, 319 were found in Berkshire selective-sweep regions reported in a previous study. Genes encompassing these nsSNPs were involved in lipid metabolism, intramuscular fatty-acid deposition, and muscle development. The effects of amino acid change by nsSNPs on protein functions were predicted using sorting intolerant from tolerant and polymorphism phenotyping V2 to reveal their potential roles in biological processes that may correlate with the unique Berkshire meat-quality traits. Conclusion: Our nsSNP findings confirmed the history of Berkshire pigs and illustrated the effects of domestication on generic-variation patterns. Our novel findings, which are generally consistent with those of previous studies, facilitated a better understanding of Berkshire domestication. In summary, we extensively investigated the relationship between genomic composition and phenotypic traits by scanning for nsSNPs in large-scale whole-genome sequencing data.

• 한국임상수의학회지
• /
• 제34권2호
• /
• pp.126-131
• /
• 2017

This study investigated the incidence rate of umbilical hernia (UH) in pig farms and examined the effect of using them as breeding pigs on reproductive traits. The occurrence of UH ranged from 0.1% to 3.0% in pig farms investigated. UH pigs were found in almost all farms except for a single farm in Jeju Island. Spontaneously occurring UH pigs were selected and used for cross breeding tests. UH-related crosses and their progeny showed significant (P < 0.05) differences in gestation period, the numbers of piglets born and alive, and body weights at birth and $21^{st}$ day comparing to those of the control population. UH-related crosses showed longer gestation period, reduced numbers of piglets, and lighter body weights than those from the control population. Interestingly, reduced number of piglets was about one fourth, suggesting that UH inheritance might play a critical role as a lethal gene during embryogenesis. In addition, UH incidence rate in UH-related crosses was significantly (P < 0.05) higher than that in the control except for UH-cross3. However, in the progeny of control cross, a pig also had UH appearance, indicating that porcine UH might be inherited in recessive inheritance mode. Taken together, the results of this study indicate that UH is one of recessively inherited genetic defect that occurs at ordinary times in pig farms, suggesting that the use of UH animals as sire and/or dam may lead to economic losses due to increased gestation period, reduced numbers of piglets born and alive, and lower growth rates after birth of pigs.

• Journal of Plant Biotechnology
• /
• 제45권4호
• /
• pp.328-332
• /
• 2018

Soybean [Glycine max (L.) Merr.] seed is an important dietary source of protein, oil, carbohydrate, isoflavone and other various nutrients for humans and animals. However, there are anti-nutritional factors in the raw mature soybeans. Kunitz trypsin inhibitor (KTI) protein and stachyose are the main anti-nutritional factors in soybean seed. The ${\alpha}^{\prime}$-subunit of ${\beta}$-conglycinin protein exhibit poor nutritional and food processing properties. The genetic removal of the KTI and ${\alpha}^{\prime}$-subunit proteins will improve the nutritional value of the soybean seed. The objective of this research was to develop a new soybean strain with KTI and ${\alpha}^{\prime}$-subunit protein free ($titicgy_1cgy_1$ genotype) and proper agronomic traits. A breeding population was developed from the cross of the Bl-1 and 15G1 parents. A total of 168 $F_2$ seeds from the cross of the BL-1 and 15G1 parents were obtained. The segregation ratios of 9: 3: 3: 1 ($104Ti\_Cgy_{1\_}:\;30Ti\_cgy_1cgy_1:\;21cgy_1cgy_1Ti\_:\;13titicgy_1cgy_1$) between the Ti and $Cgy_1$ genes in the $F_2$ seeds were observed (${\chi}^2=5.12$, P=0.5-0.10). Two $F_4$ plant strains with proper agronomical traits and $titicgy_1cgy_1$ genotype (free of both KTI and ${\alpha}^{\prime}$-subunit protein) were selected and harvested. 2 strains (S1 and S2) had yellow seed coats and hilum. The plant height of the S1 strain was 65 centimeters. The 100-seed weight was 29.2 g. The plant height of the S2 strain was 66 centimeters and 100-seed weight was 26.2 g. The two strains selected in this research will be used to improve the new cultivar that will be free of the KTI and ${\alpha}^{\prime}$-subunit proteins.

• Asian-Australasian Journal of Animal Sciences
• /
• 제32권10호
• /
• pp.1548-1557
• /
• 2019

Objective: To investigate the effects of dietary supplementation with different levels and molecular weights of fungal ${\beta}$-glucan on productive performances, health, carcass traits and meat quality in broilers. Methods: Two hundred and ten of one-day-old chicks with equal sex were assigned to seven experimental groups in $2{\times}4$ factorial arrangement. These groups were supplemented with (0, 10, 30, and 60 ppm) of molecular weight 1-3, 1-6 ${\beta}$-glucan (low or high). High molecular weight ${\beta}$-glucan (H: 943 kDa) was obtained from Ophiocordyceps dipterigena BCC 2073, whereas H with ${\gamma}$-Irradiation treatment was performed to achieve low molecular weight ${\beta}$-glucan (L: 8 kDa). Results: There was no statistical significance in productive performances, apparent digestibility and interaction between fixed factors along 42 days of experiment (p>0.05). A higher caecal amylase activity was present in the group that received L, while there was a dramatic decrease in H and the control groups, respectively (p<0.05). The increase of supplemental dose increased caecal amylase activity (p<0.05). Immunomodulatory effects from L was revealed by the marked increase of phagocytic activity, relative weight of thymus and bursa of fabricius (p<0.05). Similarly, the additive dose at 30 ppm provided the same results, whereas the only significant difference with supplementation at 60 ppm was an increase in phagocytic activity (p<0.05). Interestingly, villi height of broilers fed L was higher than other groups (p<0.05). The treatments did not influence haematology, blood chemistry, antibody production level against vaccination, carcass traits and meat quality (p>0.05). Conclusion: The supplementation of L at 30 ppm was suggested to achieve benefits of immune modulation without adverse effects on other parameters.

• Animal Bioscience
• /
• 제34권4호
• /
• pp.751-758
• /
• 2021

Objective: The objectives of this study were to investigate the relationship between the mRNA expression of adipocyte type fatty acid binding protein (A-FABP) and heart type FABP (H-FABP) in Thai native chicken crossbreeds and evaluate the level of exotic inclusion in native chicken that will improve growth while maintaining its relatively low carcass fat. Methods: The fat deposition traits and mRNA expression of A-FABP and H-FABP were evaluated at 6, 8, 10, and 12 weeks of age in 4 chicken breeds (n = 8/breed/wk) (100% Chee breed [CH] [100% Thai native chicken background], CH male and broiler female [Kaimook e-san1; KM1] [50% CH background], broiler male and KM1 female [Kaimook e-san2; KM2] [25% CH background], and broiler [BR]) using abdominal fat (ABF) and muscular tissues. Results: The BR breed was only evaluated at 6 weeks of age. At week 6, the CH breed had a significantly lower A-FABP expression in ABF and intramuscular fat (IF) compared with the other breeds. At 8 to 12 weeks, the KM2 groups showed significant upregulation (p<0.05) of A-FABP in both ABF and IF compared to the CH and KM1 groups. The expression of H-FABP did not follow any consistent pattern in both ABF and IF across the different ages. Conclusion: Some level of crossbreeding CH chickens can be done to improve growth rate while maintaining their low ABF and IF. The expression level of A-FABP correlate with most fat traits. There was no consistency of H-FABP expression across breed. A-FABPs is involved in fat deposition, genetic markers in these genes could be used in marker assisted studies to select against excessive fat accumulation.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2022년도 추계학술대회
• /
• pp.73-73
• /
• 2022

Lettuce (Lactuca sativa L., Family Asteraceae) is highly ranked in production and economic value and is consumed either fresh or in salad mixes because of its important dietary source of bioactive phytochemicals. The world collection of Lactuca spp. leafy crops, maintained in NAC, includes 2,464 accessions from 71 countries around the world, of which 2,411 belong to L. sativa species, nineteen to L. saligna, and fifteen to L. serriolar. We aimed to investigate the lettuce germplasm with morphological and biochemical analyses and provide new material for breeding. The lettuce crop comprises seven main groups of cultivars (including oilseed lettuce) differing phenotypically. Agricultural characteristcs were investigated including time to bolting, time to flowering, seed color, flower color, leaf attitude, leaf color, leaf anthocyanin coloration, type of incision of margin, depth of incisions of margins, and leaf venation. Screening of the health beneficial metabolites like anthocyanin and bitter sesquiterpene lactones (lactucin and lactucopicrin) was also conducted. The range of anthocyanin and SLs were 0~563.78 mg/100g D.W. and 3.74~3311.66 ug/g D.W., respectively. The investigation of the degree of variation regarding phenotypic traits and biochemical revealed adaptive stable and highly variable use of trait collection.

• Journal of Plant Biotechnology
• /
• 제50권
• /
• pp.115-126
• /
• 2023

A hydroponics experiment was performed to study the physiological and biochemical changes in Moroccan barley (Hordeum vulgare L.) varieties cultivated under salt stress conditions. Four barley varieties were grown under exposure to three salt concentrations, including 0, 200, and 300 mM NaCl. The ANOVA for both salt stress-sensitive and resistant varieties indicated that salt treatment represented the main source of variability in all studied traits. Salt treatment significantly reduced root and shoot dry weight (RDW and SDW), relative water content (RWC), and chlorophyll content (Chl a, Chl b, and Chl T). However, increases in electrolyte leakage (EL) along with proline and total soluble sugar (TSS) contents were recorded. In addition, large variations in all measured traits were found between varieties. The 'Massine' and 'Laanaceur' varieties displayed relatively higher RDW and SDW values. The 'Amira' and 'Adrar' varieties showed lower RWC values and Chl contents than those of the controls indicating their relative sensitivity to salt stress. Principal component analysis revealed that most of the variation was captured by PC1 (72% of the total variance) which grouped samples into three categories according to salt treatment. Correlation analyses highlighted significant associations between most parameters. Positive relationships were found between RDW, SDW, RWC, Chl content, and soluble proteins contents, while all of these parameters were negatively associated with EL intensity, proline content, and TSS content. The results from this study showed that the 'Massine' and 'Laanaceur' varieties were relatively salt-tolerant. These two salt-tolerant varieties present a good genetic background for breeding of barley varieties showing high salt tolerance.

• 생명과학회지
• /
• 제20권6호
• /
• pp.825-830
• /
• 2010

본 연구는 버크셔 품종에서 PRLR3와 RBP4 후보유전자의 두 개의 대립유전자가 산육형질과 번식형질에 미치는 영향을 조사하였다. 5,919두의 혈통자료, 3,480두의 산육기록과, 244두의 모돈의 775마리의 산자기록을 이용하여 유전능력 평가를 수행하였다. 유전자형 분석은 144두와 156두에서 PRLR3와 RBP4 유전자의 유전자형을 각각 분석하였다. 평가된 개체들의 육종가를 이용 두 마커의 유전자형 효과와 유의 확률을 추정한 결과 PRLR3 유전자는 번식형질의 총산자수(TBN)와 생존산자수(NBA)에서 -0.28과 -0.13두의 상가적 효과를 각각 나타내었다. RBP4 유전자는 일당증체량에서 10.58 g의 우성적 효과를 나타내었다. 그러나 RBP4 유전자의 다형성은 번식형질의 총 산자수(TBN)와 생존산자수(NBA)에서 -0.34와 -0.33두의 상가적 유전적 효과를 각각 나타났다. 따라서 PRLR3와 RBP의 B 대립유전자를 선호하는 MAS (Marker Assist Selection) Scheme은 버크셔 품종의 산자수의 개량에 이용 할 수 있을 것이다.

• 농업과학연구
• /
• 제14권1호
• /
• pp.51-60
• /
• 1987

본(本) 연구(硏究)는 1978년(年)부터 1983년(年)까지 국립종축원(國立種畜院)에서 사육(飼育)된 Landrace, Hampshire, Large Yorkshire 및 Duroc 종(種) 54 두(頭)의 종모돈(種牡豚)에서 생산(生産)된 253 두(頭)의 웅돈(雄豚)의 검정자료(檢定資料)를 이용(利用)하여 이유전(離乳前) 체중(體重)과 이유후(離乳後) 형질(形質)에 대한 유전력(遺傳力)과 유전상관(遺傳相關) 및 표현형상관(表現型相關)을 추정(推定)하였던바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 유전력(遺傳力) 추정치에 있어서는 이유전체중(離乳前體重)인 생시체중(生時體重), 21 일령체중(日齡體重) 및 56 일령체중(日齡體重)에서 각각(各各) $0.233{\pm}0.160$, $0.485{\pm}0.185$ 및 $0.758{\pm}0.214$이었으며, 이유후체중(離乳後體重)인 일당증체량(日當增體量), 사과요구율(飼科要求率), 등지방(脂肪)두께, 90 kg 도달일령(到達日齡) 및 선발지수(選拔指數)에서 각각(各各) $0.270{\pm}0.164$, $0.350{\pm}0.174$, $0.272{\pm}0.165$, $0.887{\pm}0.221$ 및 $0.565{\pm}0.195$이었다. 2. 유전상관(遺傳相關) 추정치(推定値)에 있어서는 생시체중(生時體重)과 21 일령체중(日齡體重) 및 56 일령체중간(日齡體重間)에 각각(各各) 0.349 및 0.198 이었으며, 21 일령체중(日齡體重)과 56 일령체중간(日齡體重間)에는 0.907이었고, 일당증체중(日當增體重)과 사료요구율(飼料要求率), 등지방층(脂肪層)두께, 90 kg 도달일령(到達日齡) 및 선발지수간(選拔指數間)의 유전상관(遺傳相關) 각각(各各) -0.552, 0.107, -0.903 및 0.71 이었으며, 사료요구율(飼料要求率)과 등지방층(脂肪層)두께, 90 kg 도달일령(到達日齡) 및 선발지수간(選拔指數間)의 유전상관(遺傳相關)은 각각(各各) -0.058, 0.699 및 -0.942이었고, 등지방층(脂肪層)두께와 90 kg 도달일령(到達日齡) 및 선발지수간(選拔指數間)의 유전상관(遺傳相關)은 각각(各各) -0.237, -0.025 이었다. 3. 표현형상관(表現型相關) 추정치(推定値)에 있어서는 생시체중(生時體重)과 21 일령체중(日齡體重) 및 56 일령체중간(日齡體重間)에 각각(各各) 0.341, 0.287이었고, 21 일령체중(日齡體重)과 56 일령체중간(日齡體重間)에는 0.893이었으며, 일당증체량(日當增體量)과 사료요구율(飼料要求率), 등지방층(脂肪層)두께, 90 kg 도달일령(到達日齡) 및 선발지수간(選拔指數間)의 표현형상관(表現型相關) 각각(各各) -0.062, 0.093, -0.651 및 0.540이었고, 사료요구율(飼料要求率)과 등지방층(脂肪層)두께, 90 kg 도달일령(到達日齡) 및 선발지수간(選拔指數間)의 표현형상관(表現型相關)은 각각(各各) 0.105, 0.601 및 -0.613이었으며, 등지방층(脂肪層)두께와 90 kg 도달일령(到達日齡) 및 선발지수간(選拔指數間)의 표현형상관(表現型相關) 각각(各各) -0.040, -0.416 이었다.