• Title/Summary/Keyword: genetic system

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Development and Application of a Cleaved Amplified Polymorphic Sequence Marker for Discriminating A Mating Type Alleles of Lentinula edodes (표고 A 교배형 구분을 위한 CAPS 마커의 개발 및 적용)

  • Park, Mi-Jeong;Ryoo, Rhim;Jang, Yeongseon;Ka, Kang-Hyeon
    • The Korean Journal of Mycology
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    • v.48 no.4
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    • pp.389-396
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    • 2020
  • Lentinula edodes is one of the most widely consumed edible mushrooms in Korea. Mating in L. edodes is regulated by a tetrapolar system, and two unlinked genetic loci, A and B, are known to be major determinants of the mating types, as reported in other heterothallic basidiomycetes. The A locus of L. edodes encodes a pair of homeodomain (HD) transcription factors. The highly variable N-termini of these HD transcription factors contribute to the diversity among the A mating types. In this study, we developed a cleaved amplified polymorphic sequence (CAPS) marker to discriminate 11 different A mating type alleles predominant among both cultivated and wild strains. Amplification of the variable region of the A locus followed by digestion with HaeIII and EcoRI restriction enzymes enabled successful discrimination among the 11 A mating type alleles. We also evaluated the applicability of this method in the identification of two A mating types of a dikaryotic strain.

Genetic Environments of Dongwon Au-Ag-bearing Hydrothermal Vein Deposit (동원 함 금-은 열수 맥상광상의 생성환경)

  • Lee, Sunjin;Choi, Sang-Hoon
    • Economic and Environmental Geology
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    • v.54 no.6
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    • pp.753-765
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    • 2021
  • The Dongwon Au-Ag deposit is located within the Paleozoic Taebaeksan province, Okcheon belt. Mineral paragenesis can be divided into two stages (stage I, ore-bearing quartz veins; stage II, barren carbonate veins) by major tectonic fracturing. Stage I, at which the precipitation of major ore minerals occurred, is further divided into three substages(early, middle and late) with paragenetic time based on minor fractures and discernible mineral assemblages: early, marked by deposition of pyrite with minor magnetite, pyrrhotite and arsenopyrite; middle, characterized by introduction of electrum and base-metal sulfides with minor sulfosalts; late, marked by argentite, Cu-As (and/or Sb) and Ag-Sb sulfosalts with base-metal sulfides. Fluid inclusion data show that stage I ore mineralization was deposited between initial high temperatures (≥430℃) and later lower temperatures (≤230℃) from fluids with salinities between 6.0 to 0.4 wt. percent equiv. NaCl. The relationship of salinity and homogenization temperature suggest that ore mineralization at Dongwon was deposited mainly due to fluid boiling, cooling and dilution via influx of cooler, more dilute meteoric waters. Changes in stage I vein mineralogy reflect decreasing temperature and fugacity of sulfur by evolution of the Dongwon hydrothermal system with increasing paragenetic time. The Dongwon deposit may represents a Korean-type and/or Au-Ag type mesothermal/epithermal gold-silver deposit.

Domain Knowledge Incorporated Counterfactual Example-Based Explanation for Bankruptcy Prediction Model (부도예측모형에서 도메인 지식을 통합한 반사실적 예시 기반 설명력 증진 방법)

  • Cho, Soo Hyun;Shin, Kyung-shik
    • Journal of Intelligence and Information Systems
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    • v.28 no.2
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    • pp.307-332
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    • 2022
  • One of the most intensively conducted research areas in business application study is a bankruptcy prediction model, a representative classification problem related to loan lending, investment decision making, and profitability to financial institutions. Many research demonstrated outstanding performance for bankruptcy prediction models using artificial intelligence techniques. However, since most machine learning algorithms are "black-box," AI has been identified as a prominent research topic for providing users with an explanation. Although there are many different approaches for explanations, this study focuses on explaining a bankruptcy prediction model using a counterfactual example. Users can obtain desired output from the model by using a counterfactual-based explanation, which provides an alternative case. This study introduces a counterfactual generation technique based on a genetic algorithm (GA) that leverages both domain knowledge (i.e., causal feasibility) and feature importance from a black-box model along with other critical counterfactual variables, including proximity, distribution, and sparsity. The proposed method was evaluated quantitatively and qualitatively to measure the quality and the validity.

Applications of Thermal Imaging Camera to Detect the Physiological States Caused by Soil Fertilizer, Shading Growth, and Genetic Characteristic (열화상 카메라 활용을 위한 토양비료, 차광생육, 유전특성 차이 관련 작물생리 원격탐지)

  • Moon, Hyun-Dong;Cho, Yuna;Jo, Euni;Kim, Hyunki;Kim, Bo-kyeong;Jeong, Hoejeong;Kwon, Dongwon;Cho, Jaeil
    • Korean Journal of Remote Sensing
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    • v.38 no.6_1
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    • pp.1101-1107
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    • 2022
  • The leaf temperature is principally regulated by the opening and closing of stomata that is sensitive to various kinds of plant physiological stress. Thus, the analysis of thermal imagery, one of remote sensing technique, will be useful to detect crop physiological condition on smart farm system and phenomics platform. However, there are few case studies using a thermal imaging camera on the agricultural application. In this study, three cases are presented: the effect of lime fertilizer on the rice, the different physiological properties of soybean under shading condition, and the screening of soybean breeds for salinity tolerance characteristic. The leaf temperature measured by thermal imaging camera on the three cases was used effectively to the physiological change and characteristics. However, the thermal imagery analysis requires considering the accuracy of measured temperature and the weather conditions that affects to the leaf temperature.

Comparison of Angelica Species Roots Using Taste Sensor and DNA Sequencing Analysis (미각센서와 DNA 염기서열을 이용한 당귀류 비교)

  • Kim, Young Hwa;Choi, Goya;Lee, Hye Won;Lee, Gwan Ho;Chae, Seong Wook;Kim, Yun Hee;Lee, Mi Young
    • The Korea Journal of Herbology
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    • v.27 no.6
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    • pp.37-42
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    • 2012
  • Objectives : Angelica Gigantis Radix is prescribed as the root of different Angelica species on the pharmacopoeia in Korea, Japan and China. Chemical components and their biological activities were also different according to their species. A study for the development of simple method to compare Angelica roots was needed. In order to classify them, the methods such as DNA sequencing analysis and taste sensor were applied to three Angelica species like Angelica gigas, Angelica acutiloba and Angelica sinensis. Methods : PCR amplification of intergenic transcribed spacer (ITS) region was performed using ITS1 and ITS4 primer from nine Angelica roots, and then nucleotide sequence was determined. Taste pattern of samples were measured using the taste-sensing system SA402B equipped with a sensing unit, which consists of artificial lipid membrane sensor probes of anionic bitterness, astringency, saltiness, umami, and cationic bitterness (C00, AE1, CT0, AAE, and AN0, respectively). Results : As a result of comparing the similarity of the ITS region sequences, A. sinensis was discriminated from the others (A. gigas and A. acutiloba). Equally this genetic result, A. gigas and A. acutiloba showed similar taste pattern as compared to A. sinensis. Sourness, bitterness, aftertaste of bitterness, astringency, and aftertaste of astringency of A. sinensis were significantly high as compared with A. gigas and A. acutiloba. In contrast, richness was significantly low. Conclusions : These taste pattern can be used as a way of comparison of Angelica species and this technic could be applied to establish a taste pattern marker for standardization of herbs in various purposes.

Neuronal Mechanisms that Regulate Vitellogenesis in the Fruit Fly (노랑초파리 난황형성과정 제어 신경 메커니즘)

  • Kim, Young-Joon;Zhang, Chen
    • Korean journal of applied entomology
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    • v.61 no.1
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    • pp.109-115
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    • 2022
  • Vitellogenesis is the process by which yolk accumulates in developing oocytes. The initiation of vitellogenesis represents an important control point in oogenesis. When females of the model insect Drosophila melanogaster molt to become adults, their ovaries lack mature vitellogenic oocytes, only producing them after reproductive maturation. After maturation, vitellogenesis stops until a mating signal re-activates it. Juvenile hormone (JH) from the endocrine organ known as the corpora allata (CA) is the major insect gonadotropin that stimulates vitellogenesis, and the seminal protein sex peptide (SP) has long been implicated as a mating signal that stimulates JH biosynthesis. In this review, we discuss our new findings that explain how the nervous system gates JH biosynthesis and vitellogenesis associated with reproductive maturation and the SP-induced post-mating response. Mated females exhibit diurnal rhythmicity in oogenesis. A subset of brain circadian pacemaker neurons produce Allatostatin C (AstC) to generate a circadian oogenesis rhythm by indirectly regulating JH and vitellogenesis through the brain insulin-producing cells. We also discuss genetic evidence that supports this model and future research directions.

Genetic Environments of Au-Ag-bearing Gasado Hydrothermal Vein Deposit (함 금-은 가사도 열수 맥상광상의 성인)

  • Ko, Youngjin;Kim, Chang Seong;Choi, Sang-Hoon
    • Economic and Environmental Geology
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    • v.55 no.1
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    • pp.53-61
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    • 2022
  • The Gasado Au-Ag deposit is located within the south-western margin of the Hanam-Jindo basin. The geology of the Gasado is composed of the late Cretaceous volcaniclastic sedimentary rocks and acidic or intermediate igneous rocks. Within the deposit area, there are a number of hydrothermal quartz and calcite veins, formed by narrow open space filling along subparallel fractures in the late Cretaceous volcaniclastic sedimentary rock. Vein mineralization at the Gasado is characterized by several textural varieties such as chalcedony, drusy, comb, bladed, crustiform and colloform. The textures have been used as exploring indicators of the epithermal deposit. Mineral paragenesis can be divided into two stages (stage I, ore-bearing quartz veins; stage II, barren carbonate veins) considering major tectonic fracturing event. Stage I, at which the precipitation of Au-Ag bearing minerals occurred, is further divided into three substages (early, middle and late) with paragenetic time based on minor fractures and discernible mineral assemblages: early, marked by deposition of pyrite and pyrrhotite with minor chalcopyrite, sphalerite and electrum; middle, characterized by introduction of electrum and base-metal sulfides with minor argentite; late, marked by argentite and native silver. Au-Ag-bearing mineralization at the Gasado deposit occurred under the condition between initial high temperatures (≥290℃) and later lower temperatures (≤130℃). Changes in stage I vein mineralogy reflect decreasing temperature and fugacity of sulfur (≈10-10.1 to ≤10-18.5atm) by evolution of the Gasado hydrothermal system with increasing paragenetic time. The Gasado deposit may represents an epithermal gold-silver deposit which was formed near paleo-surface.

Analysis of sedation and general anesthesia in patients with special needs in dentistry using the Korean healthcare big data

  • Kim, Jieun;Kim, Hyuk;Seo, Kwang-Suk;Kim, Hyun Jeong
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.22 no.3
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    • pp.205-216
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    • 2022
  • Background: People with special needs tend to require diverse behavioral management in dentistry. They may feel anxious or uncomfortable or may not respond to any communication with the dentists. Patients with medical, physical, or psychological disorders may not cooperate and therefore require sedation (SED) or general anesthesia (GA) to receive dental treatment. Using the healthcare big data in Korea, this study aimed to analyze the trends of SED and GA in special needs patients undergoing dental treatment. It is believed that these data can be used as reference material for hospitals and for preparation of guidelines and related policy decisions of associations or governments for special needs patients in dentistry. Methods: The study used selected health information data provided by the Korean National Health Insurance Service. Patients with a record of use of one of the eight selected drugs used in dental SED between January 2007 and September 2019, those with International Classification of Diseases-10 codes for attention deficit hyperactivity disorder (ADHD), phobia, brain disease, cerebral palsy, epilepsy, genetic disease, autism, mental disorder, mental retardation, and dementia were selected. The insurance claims data were analyzed for age, sex, sedative use, GA, year, and institution. Results: The number of special needs patients who received dental treatment under SED or GA from January 2007 to September 2019 was 116,623. Number of SED cases was 136,018, performed on 69,265 patients, and the number of GA cases was 56,308, implemented on 47,257 patients. In 2007, 3100 special needs patients received dental treatment under SED while in 2018 the number of cases increased 6 times to 18,528 SED cases. In dentistry, ADHD was the most common disability for SED cases while phobia was the most common cause of disability for GA. The male-to-female ratio with respect to SED cases was higher for males (M : F = 64.36% : 35.64%). Conclusion: The application of the SED method and GA for patients with special needs in dentistry is increasing rapidly; thus, preparing guidelines and reinforcing the education and system are necessary.

Improved Resource Allocation Model for Reducing Interference among Secondary Users in TV White Space for Broadband Services

  • Marco P. Mwaimu;Mike Majham;Ronoh Kennedy;Kisangiri Michael;Ramadhani Sinde
    • International Journal of Computer Science & Network Security
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    • v.23 no.4
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    • pp.55-68
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    • 2023
  • In recent years, the Television White Space (TVWS) has attracted the interest of many researchers due to its propagation characteristics obtainable between 470MHz and 790MHz spectrum bands. The plenty of unused channels in the TV spectrum allows the secondary users (SUs) to use the channels for broadband services especially in rural areas. However, when the number of SUs increases in the TVWS wireless network the aggregate interference also increases. Aggregate interferences are the combined harmful interferences that can include both co-channel and adjacent interferences. The aggregate interference on the side of Primary Users (PUs) has been extensively scrutinized. Therefore, resource allocation (power and spectrum) is crucial when designing the TVWS network to avoid interferences from Secondary Users (SUs) to PUs and among SUs themselves. This paper proposes a model to improve the resource allocation for reducing the aggregate interface among SUs for broadband services in rural areas. The proposed model uses joint power and spectrum hybrid Firefly algorithm (FA), Genetic algorithm (GA), and Particle Swarm Optimization algorithm (PSO) which is considered the Co-channel interference (CCI) and Adjacent Channel Interference (ACI). The algorithm is integrated with the admission control algorithm so that; there is a possibility to remove some of the SUs in the TVWS network whenever the SINR threshold for SUs and PU are not met. We considered the infeasible system whereby all SUs and PU may not be supported simultaneously. Therefore, we proposed a joint spectrum and power allocation with an admission control algorithm whose better complexity and performance than the ones which have been proposed in the existing algorithms in the literature. The performance of the proposed algorithm is compared using the metrics such as sum throughput, PU SINR, algorithm running time and SU SINR less than threshold and the results show that the PSOFAGA with ELGR admission control algorithm has best performance compared to GA, PSO, FA, and FAGAPSO algorithms.

Artificial Neural Network with Firefly Algorithm-Based Collaborative Spectrum Sensing in Cognitive Radio Networks

  • Velmurugan., S;P. Ezhumalai;E.A. Mary Anita
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.17 no.7
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    • pp.1951-1975
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    • 2023
  • Recent advances in Cognitive Radio Networks (CRN) have elevated them to the status of a critical instrument for overcoming spectrum limits and achieving severe future wireless communication requirements. Collaborative spectrum sensing is presented for efficient channel selection because spectrum sensing is an essential part of CRNs. This study presents an innovative cooperative spectrum sensing (CSS) model that is built on the Firefly Algorithm (FA), as well as machine learning artificial neural networks (ANN). This system makes use of user grouping strategies to improve detection performance dramatically while lowering collaboration costs. Cooperative sensing wasn't used until after cognitive radio users had been correctly identified using energy data samples and an ANN model. Cooperative sensing strategies produce a user base that is either secure, requires less effort, or is faultless. The suggested method's purpose is to choose the best transmission channel. Clustering is utilized by the suggested ANN-FA model to reduce spectrum sensing inaccuracy. The transmission channel that has the highest weight is chosen by employing the method that has been provided for computing channel weight. The proposed ANN-FA model computes channel weight based on three sets of input parameters: PU utilization, CR count, and channel capacity. Using an improved evolutionary algorithm, the key principles of the ANN-FA scheme are optimized to boost the overall efficiency of the CRN channel selection technique. This study proposes the Artificial Neural Network with Firefly Algorithm (ANN-FA) for cognitive radio networks to overcome the obstacles. This proposed work focuses primarily on sensing the optimal secondary user channel and reducing the spectrum handoff delay in wireless networks. Several benchmark functions are utilized We analyze the efficacy of this innovative strategy by evaluating its performance. The performance of ANN-FA is 22.72 percent more robust and effective than that of the other metaheuristic algorithm, according to experimental findings. The proposed ANN-FA model is simulated using the NS2 simulator, The results are evaluated in terms of average interference ratio, spectrum opportunity utilization, three metrics are measured: packet delivery ratio (PDR), end-to-end delay, and end-to-average throughput for a variety of different CRs found in the network.