• 한국양식학회지
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• 제18권4호
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• pp.252-259
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• 2005

Isozyme과 AFLP분석에 있어 종간의 유전적 차이 및 유연관계를 분석에 따른 각 유전표식의 특징 및 유용성을 파악하기 위하여 종으로 명확히 구분되는 북반구 2종(H. discus hannai와 또 rufescens)과 남반구 2종(H rubra와 H midae)을 대상으로 분석한 결과 Isozyme 및 AFLP분석은 전복류의 종간분석에 있어 유전적 차이 및 유연관계 분석에 유용한 유전표식임을 시사하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권12호
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• pp.1783-1790
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• 2007

The genetic diversity of domestic quail and two wild quail species, Japanese （Coturnix coturnix）and Common quail (Coturnix japonica), found in China was studied using microsatellite DNA markers. According to a comparison of the corresponding genetic indices in the three quail populations, such as Polymorphism Information Content (PIC), Mean Heterozygosity ($\bar{H}$) and Fixation Index, wild Common quail possessed rich genetic diversity with 4.67 alleles per site. Its values for PIC and $\bar{H}$ were the highest, 0.5732 and 0.6621, respectively. Domestic quail had the lowest values, 0.5467 and 0.5933, respectively. Wild Japanese quail had little difference in genetic diversity from domestic quail. In addition, from analyses of the fuzzy cluster based on standard genetic distance, the similarity relationship matrix coefficient between wild Japanese quail and domestic quail was 0.937, and that between wild Common quail and domestic quail was 0.783. All of these results showed that the wild Japanese quail were closer to the domestic quail for phylogenetic relationship than wild Common quail. These results at the molecular level provide useful data about quail's genetic background and further supported the hypothesis that the domestic quail originated from the wild Japanese quail.

• 농업과학연구
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• 제48권4호
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• pp.693-702
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• 2021

The aim of this study was to analyze the genetic diversity and distance of the Korean native black goat line. Thus far, this Korean native black goat line has not been studied intensively, especially in genetic diversity and relationship studies in comparison with other breeds. In total, eleven microsatellite (MS) markers were used to evaluate alleles from 391 Korean native black goats and foreign hybrid animals. The genetic diversity index was evaluated based on the allele distributions. Four Korean native black goat lines showed expected ranges of observed heterozygosity, expected heterozygosity, and polymorphism information content (PIC) values for use in genetic diversity research (0.509 - 0.643, 0.434 - 0.623 and 0.356 - 0.567). Lines from the Korean native black goat and foreign hybrid were clearly separated according to principal coordinates analysis (PCoA), phylogenetic tree and tended to be clustered in each Korean native black goat line. Thus, this study can be used for analyzing the genetic relationships between Korean native black goats and foreign breeds for line preservation and for fundamental information to determine breed improvement strategies.

• 한국환경과학회지
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• 제14권3호
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• pp.265-269
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• 2005

This study was carried out to provide the basic data for an identifying system for Iris species distributed in Korean market from complete analysing of genetic relationship between three native Iris species and one cultivar bred from the native Iris plant. RAPD analysis of genetic relationship among 4 Irises was possible. According to the RAPD analysis, they were divided into two groups. Among 4 Irises used in this study, Iris laevigata 'Veriegata', Iris laevigata and Iris setosa were classified into the same group since they had many similarities even though the habitat of Iris laevigata in Korean peninsular is restricted mainly in the south and Iris setosa is naturally inhabited in the northern part of Kangwondo. The value for the dissimilarity index of Iris laevigata and Iris laevigata 'Veriegata' was 6.757. The value for the dissimilarity index of Iris laevigata and Iris dichotoma was 95.000, so that they were genetically the farthest among them since the genetic relationship between two species are separated far if the value of the dissimilarity index is close to 100.

• 한국자원식물학회지
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• 제22권3호
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• pp.242-247
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• 2009

Randomly Amplified Polymorphic DNA (RAPD) was performed to define the genetic variation and relationships of Artemisia capillaris. Fifteen populations by the distributions and habitat were collected to conduct RAPD analysis. RAPD markers were observed mainly between 300bp and 1600bp. Total 72 scorable markers from 7 primers were applied to generate the genetic matrix, and 69 bands were polymorphic and only 3 bands were monomorphic. The genetic dissimilarity matrix by Nei's genetic distance (1972) and UPGMA phenogram were produced from the data matrix. Populations of Artemisia capillaris were clustered with high genetic affinities and cluster patterns were correlated with distributional patterns. Two big groups were clustered as southern area group and middle area group. The closest OTUs were GW2 and GG1 in middle area group, and GB1 from southern area group was clustered with OTUs in middle area group. RAPD data was useful to define the genetic variations and relationships of A. capillaris.

• Asian-Australasian Journal of Animal Sciences
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• 제21권1호
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• pp.6-10
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• 2008

Five chicken populations viz. Chittagong, Ghagus, Kalasthi, Kadaknath, Tellichery were genotyped using 25 highly polymorphic microsatellite loci. White leg horn was taken as an outgroup. To reveal the relationship and distinctiveness among five indigenous breeds various genetic distances based on molecular co-ancestry were estimated and multidimensional scaling was performed. The Ghagus and Kalasthi breeds were closely related and their separation was recent, whereas Chittagong had a remote ancestry with other indigenous chicken populations.

• Asian-Australasian Journal of Animal Sciences
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• 제22권1호
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• pp.13-19
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• 2009

In the present study, genetic analyses of diversity and differentiation were performed on four breeds of Indian zebu cattle (Bos indicus). In total, 181 animals belonging to Ponwar, Kherigarh, Gangatiri and Kenkatha breeds were genotyped for 20 cattle specific microsatellite markers. Mean number of alleles observed per locus (MNA) varied between 5.75 (Kenkatha) to 6.05 (Kherigarh). The observed and expected heterozygosity for the breeds varied from 0.48 (Gangatiri) to 0.58 (Kherigarh) and 0.65 (Kenkatha) to 0.70 (Kherigarh), respectively. $F_{IS}$ estimates of all the breeds indicated significant deficit of heterozygotes being 28.8%, 25.9%, 17.7% and 17.7% for Gangatiri, Ponwar, Kherigarh and Kenkatha, respectively. The $F_{ST}$ estimates demonstrated that 10.6% was the average genetic differentiation among the breeds. Nei's genetic distance DA and Cavalli- Sforza and Edwards Chord distance ($D_C$) and the phylogenetic tree constructed from these reflected the close genetic relationship of Gangatiri and Kenkatha, whereas Ponwar appears to be more distant.

• Asian-Australasian Journal of Animal Sciences
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• 제32권2호
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• pp.176-182
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• 2019

Objective: This study was conducted to investigate basic information on genetic structure and characteristics of Limousin population in Hungary. Obtained results will be taken into consideration when adopting the new breeding strategy by the Association of Hungarian Limousin and Blonde d'Aquitaine Breeders (AHLBB). Methods: Genetic diversity and phylogenetic relationship of 3,443 Limousin cattle from 16 different herds were investigated by performing genotyping using 18 microsatellite markers. Amplified DNA was genotyped using an automated genetic analyzer. Results: Mean of effective alleles ($n_e$) of the populations was 3.77. Population C had the lowest number of effective alleles (3.01) and the lowest inbreeding coefficient ($F_{IS}$) value (-0.15). Principal component analysis of estimated genetic distance ($F_{ST}$) values (p<0.000) revealed two herds (C and E) distinct from the majority of other Limousin herds. The pairwise $F_{ST}$ values of population C compared to the others (0.066 to 0.120) fell into the range of moderate genetic distance: 0.050 to 0.150, while population E displayed also moderate genetic distance ($F_{ST}$ values in range 0.052 to 0.064) but only to six populations (G, H, J, L, N, and P). $F_{ST(C-E)}$ was 0.148, all other pairs -excluding C and E herds- displayed low genetic distance ($F_{ST}$<0.049). Population D, F, I, J, K, L, N, O, and P carried private alleles, which alleles belonged to 1.1% of the individuals. Most probable number of clusters (K) were 2 and 7 determined by Structure and BAPS software. Conclusion: This study showed useful genetic diversity and phylogenetic relationship data that can be utilized for the development of a new breeding strategy by AHLBB. The results presented could also contribute to the proper selection of animals for further whole genome scan studies of Limousins.

• Asian-Australasian Journal of Animal Sciences
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• 제18권8호
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• pp.1071-1074
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• 2005

Microsatellite polymorphism and their genetic relationships were estimated using genotype information of 183 dogs from 11 microsatellite loci. The breeds include the indigenous Korean breeds Jindo dog (30), Poongsan dog (20) and Miryang dog (44) together with Chihauhau dog (31) and German Shepherd dog (58). Jindo dogs showed the highest expected heterozygosity (0.796${\pm}$0.030) and polymorphic information contents (0.755) in all populations. The phylogenetic analysis showed the existence of two distinct clusters supported by high bootstrap values: the Korean native dogs and other dogs. They clearly show that Poongsan dog and Miryang dog are closely related to each other when compared with Jindo dog. Microsatellite polymorphism data was shown to be useful for estimating the genetic relationship between Korean native dogs and other dog breeds, and also can be applied for parentage testing in those dog breeds.

• 한국산림과학회지
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• 제87권2호
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• pp.153-163
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• 1998

사시나무속(屬)(Genus Populus) Leuce절 수종중 우리나라에 식재되어 있는 사시나무, 수원사시나무, 은백양, 은사시나무와 인공교배종 수개 클론에 대한 분자유선학적 유연관계를 RAPD PCR 방법을 이용하여 구명하였다. 88개의 arbitrary primer중 재현성과 다형성을 기준으로 선발하고, 유연관계분석을 위하여 22개의 primer에서 181개의 RAPD marker를 이용하였다. 유연관계를 위한 조사는 5개 수종, 14개 클론 몇 천연집단의 개체목에 대하여 181개의 다형성 RAPD marker를 가지고, UPGMA와 Neighbor-joining 방법으로 유연관계도를 구했다. 방법을 달리하여 그런 유연관계도에서 각각의 분지에서의 차이는 현 사시 클론간에 미미한 위치변화만 있을 뿐 전체적인 계통수에는 변화가 없었다. 유연관계도에서 수원사시나무는 은백양과 같은 분지군을 형성하였고, 주성분분석에서는 사시나무와 같은 계열을 이루고 있어서 수원사시나무는 이 들 두 종의 1대 교잡종으로 추정되며, 은사시나무는 자연교잡종과 인공교배종이 동일한 유전적 배경을 갖는 것으로 나다났다.