• Journal of Microbiology
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• v.41 no.1
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• pp.1-6
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• 2003

The genetic relatedness of multidrug-resistant pneumococcal isolates of serotypes 19F and 23F was investigated. The DNA fragments digested with Sma I were resolved by pulsed-field gel electrophoresis (PFGE). PFGE analysis of 365. pneumoniae isolates showed 13 different patterns. Among 22 isolates of serotype 19F, 9 different PFGE patterns were present and 14 isolates of serotype 23F isolates represented 5 distinct PFGE patterns. Two isolates of serotype 19F and six isolates of serotype 23F shared the same PFGE pattern (Pattern I). Based on the genetic relatedness within the strains (one genetic cluster was defined as having more than 85% homology), we divided the pneumococcal strains into genefic clusters (Ⅰ, II, III, IV, V, and VI). The 22 strains of serotype 19F belonged to five distinct genetic clusters (I, II, III, IV, V and VI) and 14 strains of serotype 23F represented two genetic clusters (I and II ). These results showed that strains of serotype 19F are genetically more diverse than those of serotype 23F, Serotype 19F isolates with PFGE patterns H and I appeared to be less related to those of the remaining PFCE patterns (A to G) (less than 60% genetic relatedness), but those strains were genetically closely related with serotype 23f. These results suggest that the latter isolates originated from horizontal transfer of the capsular type 19F gene locus to 23F pneumococcal genotypes. In conclusion, the multidrug-resistant pneumococcal isolates of serotype 19f and 23F isolated in Korea are the result of the spread of a limited number of resistant clones.

• Korean Journal Plant Pathology
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• v.10 no.1
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• pp.1-6
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• 1994

Randomly chosen recombinant clones of Fusarium oxysporum were analysed to select useful probes for relatedness analysis of Fusarium oxysporum. Genomic DNA of F. oxysproum f. sp. cubense, digested with HindIII, was ligated to pUC118 and used to transform Escherichia coli strai DH5$\alpha$. Three clones were identified that hybridized to mutiple restriction fragments of some formae speciales of F. oxysporum. These probes detected repetitive sequences in HindIII or EcoRI digested DNAs. Repeated copy clone pFC46, pFC52 and pFC54 showed evident polymorphisms among ten formae speciales of this fungus. Since clone pFC 52 strongly hybridized to multiple EcoRI-digested restriction fragments of f. sp. cubense, it may be useful as a probe for analysis of other genetic characteristics of this forma specialis. The results suggest that our clones might be very useful as probes for relatedness analysis between or within formae speciales of Fusarium oxysporum.

• Korean Journal of Environment and Ecology
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• v.11 no.3
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• pp.277-289
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• 1997

Electrophresis methods were used to examined the degree of genetic relatedness and divergent times on the 4 genera 4 species of the family Corvidae and 1 species of the Family Cinclidae. Genetic relatedness between two families and between 4 genera of Corvidae showed similar to those reported at comparable taxonomic levels in other bird. But it appears to be considerably less than that of non avian taxa. Genetic relatedness between Garrulus glandarius brandtii and Corvus corone orientalis was the most close related(S=0.81), whereas Pica pica sericea(S=0.72) and Cyanopica cyanus koreensis(S=0.68) were showed less close relatedness respectively. The presumed divergent time between two families was 3.9 million years before present. Whereas among the 4 species of corvidae, the presumes divergent times of Cyanopica cyanus koreensis and Pica pica sericea were about 1.9 and 1.6 million years before present respectively, and between Garrulus glandarius brandtii and Corvus corone orientalis was showed recently about 1.0 million years before present

• Mycobiology
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• v.30 no.1
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• pp.1-4
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• 2002

Genetic relatedness of medically important Exophiala species such as E. dermatitidis, E. mansonii, and three E. jeanselmei varieties: jeanselmei, lecanii-corni, and heteromorpha was examined using PCR-RFLP(restriction fragment length polymorphism) of ribosomal DNA, M-13, $(GTG)_5$ and nucleotide sequences of ribosomal ITS(internal transcribed space) II regions. Three E. jeanselmei varieties showing distinct band patterns for each DNA markers as well as different nucleotide sequences of ribosomal ITS II regions could be considered as a separate species. E. dermatitidis and E. mansonii demonstrated the identical band patterns of RFLP of ribosomal DNA, M-13, and $(GTG)_5$ markers. However, nucleotides sequences of ribosomal ITS II region were different between these two species.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.11
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• pp.1535-1541
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• 2005

Five broiler chicken lines, namely HC, BPB2, CPB2, PB2 and UM1, involving in a selection program and differing in selection intensity and genetic background, were screened for randomly amplified polymorphic DNA (RAPD) polymorphism using 10 selected decamer primers. Nine primers amplified the genomic DNA, generating 200 to 2,500 bp and all detected polymorphism between lines. Out of 74 bands scored using these primers, 34 (50.0%) were found to be polymorphic. The number of polymorphic loci ranged from 3 to 6 with an average of 4.33. Lines differed considerably for within-population genetic similarity estimated by band frequency (WS = 93.55 to 99.25). Between-line genetic similarity estimates based on band sharing as well as on band frequency ranged from 71.35 to 86.45 and from 73.38 to 87.68, respectively. Lines HC and PB2 were the most closely related to the other, while BPB2 and CPB2 appeared to be more distant from each other. The between-line genetic distance based on both band sharing and band frequency revealed the similar trends as for Between-line genetic similarity. Based on BS and BF criteria, BPB2 and CPB2 as well as PB2 and UM1 lines can be merged to launch a new genetic group for further progress in biometrical objectives. A phylogenetic tree, derived using Nei's coefficient of similarity revealed the different pattern of genetic distance between lines.

• Animal Systematics, Evolution and Diversity
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• v.6 no.1
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• pp.17-28
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• 1990

Electrophoretic methods were used to examine the degree of genic variation and genetic relatedness in 11 populations representing 6 species and subspecies of the genus Parus in Korea. The average degree of genic variation of 3 subspecies of P. major was =1.2, =24.4% , D=0.042, and G=0.058, whereas the rest of the species showed slightly lower degree of genic variation than P. major. Genetic relatedness between subspecies and species in the genus Parus showed similar to those reported at comparable taxnonomic levels in other birds. But it appears to be considerably less than that of non avian taxa. Genetic relatedness between 3 subspecies of P.major and P. varius varius was closely related(=0.80), whereas between P.palustrius hellmayri and P.ater amurensis was relatively remote (=0.67). The presumed divergent times of P.palustrius hellmayri, P, ater amurensis , and P. varius varius were about 1.8, 1.6, and 1.0million years before present respectively, and 3 subspecies of P. major were recently differentiated about 100 thousands years before present.

• Journal of Species Research
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• v.1 no.1
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• pp.100-103
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• 2012

Nutria Myocastor coypus is one of a well known invasive riparian mammal found species around world from North America to Eurasia and Africa. In South Korea, feral nutrias inhabit areas from the Nakdonggang and Namgang (River) to their tributaries and Upo Wetland where they have had devastating effects on environment. Nevertheless, there has been little research about nutrias in Korea. This study is to analyze the genetic structure of the nutria population in the Upo Wetland and identify the origin of the source populations. Twenty individuals from the Upo Wetland were genotyped using 25 microsatellite loci. When compared with another introduced population, that of the Blackwater Nation Wildlife Refuge in U.S., the Upo population contains considerable genetic variations. Tests for Hardy-Weinberg equilibrium and Bayesian clustering analysis suggest the Upo population is genetically structured and has at least two source populations. This preliminary study presents the need for further in-depth studies about this species which should combine genetic and ecological studies.

• Journal of the Korean Data and Information Science Society
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• v.17 no.4
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• pp.1041-1051
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• 2006

The effectiveness of genetic markers on Hanwoo traceability systems was applied and evaluated from Korean 33 Hanwoo elite sire families. Five microsatellite markers were selected finally, which were located on chromosomes different chromosomes with the end sequencing of 100 HW-YUBAC that were recorded in the NCBI by Yeungnam University. Eleven major microsatellite markers were selected from allele amplified, their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the power of the markers selected on the individual animal identification with experimental condition, the match probability(MP) and the relatedness coefficient(R) were computed.

• Communications for Statistical Applications and Methods
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• v.13 no.3
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• pp.733-743
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• 2006

To apply and evaluate the effectiveness of genetic markers on Hanwoo traceability systems, samples of 33 Hanwoo individuals from Korean elite sire families were used, and five microsatellite markers were selected finally, which were located on chromosomes different chromosomes with the end sequencing of 100 HW-YUBAC that were recorded in the NCBI by Yeungnam University. Ten major microsatellite markers were selected from alleles amplified, their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the power of the markers selected on the individual animal identification, the match probability(MP) and the relatedness coefficient(R) were computed.

• Journal of the military operations research society of Korea
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• v.28 no.2
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• pp.116-124
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• 2002

In just-in-time production systems, U-shaped production lines rather than traditional straight lines are often adopted since they have some advantages. The advantages of U-lines over straight lines are that the workstations required can be reduced and the necessary number of workers can be easily adjusted when the demand rates are changed. In this paper, we present a new genetic algorithm(GA) to minimize the number of workstations primarily and improve the work relatedness secondarily in the U-line production systems. Also, a new heuristic method is presented according to the work related factors and characteristics of U-line balancing. Some major aspects of the proposed GA are discussed, with emphasis on representation, decoding and evaluation function. Extensive experiments are carried out on well-known test-bed problems in the literature to verify the performance of our algorithm . The computational results show that our algorithm is a promising alternative to existing heuristics.