• Title/Summary/Keyword: general interference

Search Result 285, Processing Time 0.036 seconds

Structural basis of novel TRP14, thioredoxin-related protein that regulates TNE-$\alpha$ signaling pathways

  • Woo, Joo-Rang;Jeong, Woo-Jin;Rhee, Sue-Goo;Ryu, Seong-Eon
    • Proceedings of the Korea Crystallographic Association Conference
    • /
    • 2003.05a
    • /
    • pp.18-18
    • /
    • 2003
  • Thioredoxin (Trx) is a small redox protein that is ubiquitously distributed from achaes to human. In diverse organisms, the protein is involved in various physiological roles by acting as electron donor and regulators of transcription and apoptosis as well as antioxidants. Sequences of Trx within various species are 27~69% identical to that of E. coli and all Trx proteins have the same overall fold, which consists of central five β strands surrounded by four α helices. The N-terminal cysteine in WCGPC motif of Trx is redox sensitive and the motif is highly conserved. Compared with general cysteine, the N-terminal cysteine has low pKa value. The result leads to increased reduction activity of protein. Recently, novel thio.edoxin-related protein (TRP14) was found from rat brain. TRP14 acts as disulfide reductase like Trx1, and its redox potential and pKa are similar to those of Trx1. However, TRP14 takes up electrons from cytosolic thioredoxin reductase (TrxR1), not from the mitochondrial thioredoxin reductase (TrxR2). Biological roles of TES14 were reported to be involved in regulating TNF-α induced signaling pathways in different manner with Trx1. In depletion experiments, depletion of TRP14 increased TNF-α induced phosphorylation and degradation of IκBα more than the depletion Trx1 did. It also facilitated activation of JNK and p38 MAP kinase induced by TNF-α. Unlike Trx1, TRP14 shows neither interaction nor interference with ASK1. Here, we determined three-dimensional crystal structure of TRP14 by MAD method at 1.8Å. The structure reveals that the conserved cis-Pro (Pro90) and active site-W-C-X-X-C motif, which may be involved in substrate recognition similar to Trx1 , are located at the beginning position of strand β4 and helix α2, respectively. The TRP14 structure also shows that surface of TRP14 in the vicinity of the active site, which is surrounded by an extended flexible loop and an additional short a helix, is different from that of Trx1. In addition, the structure exhibits that TRP14 interact with a distinct target proteins compared with Trx1 and the binding may depend mainly on hydrophobic and charge interactions. Consequently, the structure supports biological data that the TRP14 is involved in regulating TNF-α induced signaling pathways in different manner with Trx1.

  • PDF

A Productivity Analysis Method of Curtain Wall Works Using Construction Simulation (건설 시뮬레이션을 활용한 커튼월 적층공법의 생산성 분석방안)

  • Park, Dong-Geun;Lee, Kyung-Suk;Yu, Byung-In;Kim, Young-Suk;Han, Seung-Woo
    • Proceedings of the Korean Institute Of Construction Engineering and Management
    • /
    • 2008.11a
    • /
    • pp.256-261
    • /
    • 2008
  • The curtain-wall work has been more frequently applied in the construction industry since demand of high-rise buildings has been increased. The curtain-wall work is usually performed with the frame work simultaneously for reducing construction period, but it might be delayed because of several problems caused by interference of process. However, there is not an appropriate tool which can be used by a work manager for adjusting quantity of the construction equipments or the workers when the curtain-wall work was delayed. To resolve this problem a construction simulation anticipating and analyzing potential problems before starting the work can be applied in the curtain wall work. This research suggests a general model for the curtain-wall work by using construction simulation and produces a combination of construction equipments and workers which can estimate optimum work productivity.

  • PDF

Validated HPLC Method for the Pharmacokinetic Study of Atenolol and Chlorthalidone Combination Therapy in Korean Subjects

  • Kang, Hyun-Ah;Kim, Hwan-Ho;Kim, Se-Mi;Yoon, Hwa;Cho, Hea-Young;Oh, Seaung-Youl;Choi, Hoo-Kyun;Lee, Yong-Bok
    • Journal of Pharmaceutical Investigation
    • /
    • v.36 no.5
    • /
    • pp.331-338
    • /
    • 2006
  • A rapid, selective and sensitive reverse-phase HPLC methods for the determination of atenolol and chlorthalidone in human serum and whole blood were validated, and applied to the pharmacokinetic study of atenolol and chlorthalidone combination therapy. Atenolol and an internal standard, pindolol, were extracted from human serum by liquid-liquid extraction, and analyzed on a $\mu$-Bondapak C18 $10-{\mu}$ column in a mobile phase of methanol-0.01 M potassium dihydrogenphosphate(30:70, v/v, adjusted to pH 3.5) and fluorescence detection(emission: 300 nm, excitation: 224 nm). Chlorthalidone and an internal standard, probenecid, were extracted form human whole blood by liquid-liquid extraction, and analyzed on a Luna C18 $5-{\mu}$ column in a mobile phase of acetonitrile containing 77% 0.01 M sodium acetate and UV detection at 214 nm. These analysis were performed at three different laboratories using the same quality control(QC) samples. The chromatograms showed good resolution, sensitivity, and no interference by human serum and whole blood, respectively. The methods showed linear responses over a concentration range of 10-1,000 ng/mL for atenolol and 0.05-20 ${\mu}g/mL$ for chlorthalidone, with correlation coefficients of greater than 0.999 at all the three laboratories. Intra- and inter-day assay precision and accuracy fulfilled international requirements. Stability studies(freeze-thaw, short-, long-term, extracted sample and stock solution) showed that atenolol and chlorthalidone were stable. The lower limit of quantitation of atenolol and chlorthalidone were 10 ng/mL and 0.05 ${\mu}g/mL$, respectively, which was sensitive enough for pharmacokinetic studies. These methods were applied to the pharmacokinetic study of atenolol and chlorthalidone in human volunteers following a single oral administration of Hyundai $Tenoretic^{\circledR}$ tablet(atenolol 50 mg and chlorthalidone 12.5 mg) at three different laboratories.

Back-scattering Characteristic Analysis for SAR Calibration Site (SAR 검보정 Site 구축을 위한 후방 산란 특성 분석)

  • Lee, Taeseung;Yang, Dochul
    • Korean Journal of Remote Sensing
    • /
    • v.37 no.2
    • /
    • pp.305-319
    • /
    • 2021
  • The overseas calibration sites such as Mongolia used for Korea Multi-purpose Satellite (KOMPSAT-5 or K5), have a disadvantage in that maintenance and repair costs are high and immediate response is difficult when an unexpected problem occurs. Accordingly, the necessity of establishing a domestic SAR calibration site was suggested, but the progress of related research is insignificant. In this paper, we investigated what conditions should be satisfied in terms of backscattering characteristics to construct a site for SAR satellite image quality evaluation and calibration. First of all, it was selected first by applying general indicators such as accessibility and availability among places recommended as satellite image calibration candidate sitesin Korea. Next, three places, site A (Goheung-gun, Jeollanam-do), site B (Jeonju-si, Jeollabuk-do), and site C (Daedeok Research Complex, Daejeon), were selected as the final candidates because they are relatively wide and easy to install AT or CR. Site A, located in Goheung-gun, Jeollanam-do, was best considered in terms of slope measurements, minimum site area to obtain ISLR, uniformity of DN values and backscatter coefficients, interference by strong reflectors, and backscatter clutter level.

An Aerodynamic Modeling and Simulation of a Folding Tandem Wing Type Aerial Launching UAV (접이식 직렬날개형 공중투하 무인비행체의 공력 모델링 및 시뮬레이션)

  • Lee, Seungjin;Lee, Jungmin;Ahn, Jeongwoo;Park, Jinyong
    • Journal of the Korea Society for Simulation
    • /
    • v.27 no.4
    • /
    • pp.19-26
    • /
    • 2018
  • The aerial launching UAV(Unmanned Aerial Vehicle) mainly uses a set of folding tandem wings to maximize flight performance and minimize the space required for mounting in a mothership. This folding tandem wing has a unique aerodynamic problem that is different from the general type of fixed wing aircraft, such as the rear wing interference problem caused by the wing of the front wing wake and vortex, and the imbalance of the pivot moment applied to the front and rear wings when the wing is deployed. In this paper, we have modeled and simulated various cases through computational fluid dynamics based on the finite volume method and analyzed various aerodynamic phenomena of the tandem wing type aircraft. We find that the front wing shall be installed higher than the rear for minimizing the wake influence and the rear wing can be deployed faster than the front because of the pivot moment due to aerodynamic forces. Also, considering the pivot moment due to aerodynamic force, the rear wing can be deployed much faster than the front wing. Therefore, it is necessary to consider it when developing the wing deploy mechanism.

A Study on the Error Rate of Non-destructive Rebar Detection Under Different Environmental Factors (환경적 요인에 따른 비파괴 철근 탐사의 오차율에 관한 연구)

  • Kang, Beom-Ju;Kim, Young-Hwan;Kim, Young-Min;Park, Kyung-Han;Oh, Hong-Seob
    • Journal of the Korean Recycled Construction Resources Institute
    • /
    • v.9 no.4
    • /
    • pp.506-513
    • /
    • 2021
  • The durability and safety of reinforced concrete structures significantly depend on the reinforcement conditions, concrete cover thickness, cracks, and concrete strength. There are two ways to accurately determine the information on reinforcing bars embedded in concrete - the local destructive method and the non-destructive rebar detection test. In general, the non-destructive rebar detection tests, such as the electromagnetic wave radar method, electromagnetic induction method, and radiation method, are adopted to avoid damage to the structural elements. The moisture content and temperature of concrete affect the dielectric constant, which is the electrical property of concrete, and cause interference in the non-destructive rebar detection test results. Therefore, in this study, the effects of the electromagnetic wave radar method and electromagnetic induction method have been analyzed according to the temperature and surface moisture content of concrete. Due to the technological advancement and development of equipment, the average error rate was less than 5% in the specimens at 24℃, irrespective of their operating principles. Among the tested methods, the electromagnetic induction method showed very high accuracy. The electromagnetic wave radar method indicated a relatively small error rate in the dry state than in the wet state, and exhibited a relatively high error rate at high temperatures. It was confirmed that the error could be reduced by applying the electromagnetic wave radar method when the temperature of the probe was low and in a dry state, and by using the electromagnetic induction method when the probe was in a wet state or at a high temperature.

Pollen Analysis on Tangjeong Plains, Asan-Si, Korea (아산(牙山) 탕정평야(湯井平野)의 화분분석(花粉分析))

  • PARK, Ji-Hoon;JANG, Dong-Ho
    • Journal of The Geomorphological Association of Korea
    • /
    • v.17 no.2
    • /
    • pp.53-61
    • /
    • 2010
  • In this study, pollen analysis and a dating were performed on the alluvial deposits in the Tangjeong Plains in Asan-Si, Chungcheongnam-Do. Specimens were collected at the altitude of about 12m, which belongs to the mid-forest belt in the cool temperate zone. The followings show the results. In general, the target zone passed through TJ-I (the coniferous forest age in which the Pinus forest was dominant), TJ-II (the mixed conifer and deciduous broad-leaved forest age in which Pinus and Quercus were dominant) and TJ-III (the coniferous forest age in which the Pinus was dominant) respectively. TJ-II was subdivided into TJ-IIa and TJ-IIb. TJ-I is presumed to be between about 2,810 and 1,500yrB.P.; TJ-IIa to be between about 1,500 and 1,370yrB.P.; and TJ-IIb to be between about 1,370 and 770yrB.P. As for TJ-III, it is presumed to begin after about 770yrB.P. In comparison with the nationwide pollen zone during the Postglacial, TJ-I and TJ-II are contrasted with the R-IIIa period and also TJ-III is contrasted with RIIIb (so-called human interference age). It is also presumed that Pinus luxuriated there after about 770yrB.P. as forests began to be markedly destroyed in the Tangjeong Plains.

Evaluation of Performance of Grouts and Pipe Sections for Closed-loop Vertical Ground Heat Exchanger by In-situ Thermal Response Test (현장 열응답 시험을 통한 수직 밀폐형 지중열교환기용 그라우트와 열교환 파이프 단면의 성능 평가)

  • Lee, Chul-Ho;Park, Moon-Seo;Min, Sun-Hong;Choi, Hang-Seok;Sohn, Byong-Hu
    • Journal of the Korean Geotechnical Society
    • /
    • v.26 no.7
    • /
    • pp.93-106
    • /
    • 2010
  • In performing a series of in-situ thermal response tests, the effective thermal conductivities of six vertical closed-loop ground heat exchangers were experimentally evaluated and compared one another, which were constructed in a test bed in Wonju. To compare thermal efficiency of the ground heat exchangers in field, the six boreholes were constructed with different construction conditions: grouting materials (cement vs. bentonite), different additives (silica sand vs. graphite) and the shape of pipe-sections (general U-loop type vs. 3 pipe-type). From the test results, it can be concluded that cement grouting has a higher effective thermal conductivity than bentonite grouting, and the efficiency of graphite better performs than silica sand as a thermally-enhancing addictive. In addition, a new 3 pipe-type heat exchanger provides less thermal interference between the inlet and outlet pipe than the conventional U-loop type heat exchanger, which results in superior thermal performance. Based on the results from the in-situ thermal response tests, a series of economic analyses have been made to show the applicability of the new addictives and 3 pipe-type heat exchanger.

Aspects of Korean rhythm realization by second language learners: Focusing on Chinese learners of Korean (제 2언어 학습자의 한국어 리듬 실현양상 -중국인 한국어 학습자를 중심으로-)

  • Youngsook Yune
    • Phonetics and Speech Sciences
    • /
    • v.15 no.3
    • /
    • pp.27-35
    • /
    • 2023
  • This study aimed to investigate the effect of Chinese on the production of Korean rhythm. Korean and Chinese are typologically classified into different rhythmic categories; because of this, the phonological properties of Korean and Chinese are similar and different at the same time. As a result, Chinese can exert both positive and negative influences on the realization of Korean rhythm. To investigate the influence of the rhythm of the native language of L2 learners on their target language, we conducted an acoustic analysis using acoustic metrics like of the speech of 5 Korean native speakers and 10 advanced Chinese Korean learners. The analyzed material is a short paragraph of five sentences containing a variety of syllable structures. The results showed that KS and CS rhythms are similar in %V, VarcoV, and nPVI_S. However, CS, unlike KS, showed characteristics closer to those of a stress-timed language in the values of %V and VarcoV. There was also a significant difference in nPVI_V values. These results demonstrate a negative influence of the native language in the realization of Korean rhythm. This can be attributed to the fact that all vowels in Chinese sentence are not pronounced with the same emphasis due to neutral tone. In this sense, this study allowed us to observe influences of L1 on L2 production of rhythm.

Some New Problems of International Aviation Security- Considerations Forcused on its Legal Aspects (최근국제항공보안대책(最近國際航空保安対策)의 제간제(諸間題) -특히 법적측면(法的測面)을 중심(中心)으로-)

  • Choi, Wan-Sik
    • The Korean Journal of Air & Space Law and Policy
    • /
    • v.5
    • /
    • pp.53-75
    • /
    • 1993
  • This article is concerned with the comment on "Some New Problems of International Aviation Security-Considerations Forcused on its Legal Aspects". Ever since 1970, in addition to the problem of failure to accept the Tokyo, Hague and Montreal Conventions, there has been also the problem of parties to them, failing to comply with their obligations under the respective treaties, in the form especially of nominal penalties or the lack of any effort to prosecute after blank refusals to extradite. There have also been cases of prolonged detention of aircraft, passengers and hostages. In this regard, all three conventions contain identical clauses which submit disputes between two or more contracting States concerning the interpretation or application of the respective conventions to arbitration or failing agreement on the organization of the arbitration, to the International Court of Justice. To the extent to which contracting States have not contracted out of this undertaking, as I fear they are expressly allowed to do, this promision can be used by contracting States to ensure compliance. But to date, this avenue does not appear to have been used. From this point of view, it may be worth mentioning that there appears to be an alarming trend towards the view that the defeat of terrorism is such an overriding imperative that all means of doing so become, in international law, automatically lawful. In addition, in as far as aviation security is concerned, as in fact it has long been suggested, what is required is the "application of the strictest security measures by all concerned."In this regard, mention should be made of Annex 17 to the Chicago Convention on Security-Safeguarding International Civil Aviation against Acts of Unlawful Intereference. ICAO has, moreover, compiled, for restricted distribution, a Security Manual for Safeguarding Civil Aviation Against Acts of Unlawful Interference, which is highly useful. In this regard, it may well be argued that, unless States members of ICAO notify the ICAO Council of their inability to comply with opecific standards in Annex 17 or any of the related Annexes in accordance with Article 38 of the 1944 Chicago Convention on International Civil Aviation, their failure to do so can involve State responsibility and, if damage were to insure, their liability. The same applies to breaches of any other treaty obligation. I hope to demonstrate that although modes of international violence may change, their underlying characteristics remain broadly similar, necessitating not simply the adoption of an adequate body of domestic legislation, firm in its content and fairly administered, but also an international network of communication, of cooperation and of coordination of policies. Afurther legal instrument is now being developed by the Legal Committee of ICAO with respect to unlawful acts at International airports. These instruments, however, are not very effective, because of the absence of universal acceptance and the deficiency I have already pointed out. Therefore, States, airports and international airlines have to concentrate on prevention. If the development of policies is important at the international level, it is equally important in the domestic setting. For example, the recent experiences of France have prompted many changes in the State's legislation and in its policies towards terrorism, with higher penalties for terrorist offences and incentives which encourage accused terrorists to pass informations to the authorities. And our government has to tighten furthermore security measures. Particularly, in the case an unarmed hijacker who boards having no instrument in his possession with which to promote the hoax, a plaintiff-passenger would be hard-pressed to show that the airline was negligent in screening the hijacker prior to boarding. In light of the airline's duty to exercise a high degree of care to provide for the safety of all the passengers on board, an acquiescence to a hijacker's demands on the part of the air carrier could constitute a breach of duty only when it is clearly shown that the carrier's employees knew or plainly should have known that the hijacker was unarmed. The general opinion is that the legal oystem could be sufficient, provided that the political will is there to use and apply it effectively. All agreed that the main responsibility for security has to be borne by the governments. A state that supports aviation terrorism is responsible for violation of International Aviation Law. Generally speaking, terrorism is a violation of international law. It violates the sovereign rights of states, and the human rights of the individuals. We have to contribute more to the creation of a general consensus amongst all states about the need to combat the threat of aviation terrorism. I think that aviation terrorism as becoming an ever more serious issue, has to be solved by internationally agreed and closely co - ordinated measures.

  • PDF