• Journal of Environmental Impact Assessment
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• v.14 no.6
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• pp.387-402
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• 2005

In this study, we tried to predict the change of future land-cover and relationships between land-cover change and geo-spatial information in the Gongju area by using fuzzy logic operation. Quantitative evaluation of prediction models was carried out using a prediction rate curve using. Based on the analysis of correlations between the geo-spatial information and land-cover change, the class with the highest correlation was extracted. Fuzzy operations were used to predict land-cover change and determine the land-cover prediction maps that were the most suitable. It was predicted that in urban areas, the urban expansion of old and new towns would occur centering on the Gem-river, and that urbanization of areas along the interchange and national roads would also expand. Among agricultural areas, areas adjacent to national roads connected to small tributaries of the Gem-river and neighboring areas would likely experience changes. Most of the forest areas are located in southeast and from this result we can guess why the wide chestnut-tree cultivation complex is located in these areas and the possibility of forest damage is very high. As a result of validation using the prediction rate curve, it was indicated that among fuzzy operators, the maximum fuzzy operator was the most suitable for analyzing land-cover change in urban and agricultural areas. Other fuzzy operators resulted in the similar prediction capabilities. However, in the prediction rate curve of integrated models for land-cover prediction in the forest areas, most fuzzy operators resulted in poorer prediction capabilities. Thus, it is necessary to apply new thematic maps or prediction models in connection with the effective prediction of changes in the forest areas.

• Korean Journal of Plant Tissue Culture
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• v.24 no.2
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• pp.93-97
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• 1997

For the molecular genetic study of cold tolerance mechanism in plants, a cDNA encoding fatty acid desaturase (fad3), converting linoleic acid (18:2, $\omega$-6) to linolenic acid (18:3, $\omega$-3), was isolated from $\lambda$ZAPII Arabidopsis thaliana cDNA expression library by plaque hybridization using fad3 cDNA probe derived from Brassica napus. A 1.8 kb-EcoRI fragment from a lambda clone showing a strong positive hybridization signal was subcloned into pGEM7 and analyzed for its nucleotide sequence. From deduced amino acid sequences, the fad3 gene was revealed to have an open reading frame(ORF) consisting of 386 amino acids with a molecular mass of 44,075 Da. The fad3 gene was compared to chloroplast $\omega$-3 fatty acid desaturase (fad7) and endoplasmic reticulum Δ12 fatty acid desaturase (fad2) to show 70% and 58% amino acid sequence homology, respectively, Especially, amino acids of internal (82 to 151) and carboxy terminal (276 to 333) regions were highly conserved, implying their requisite role for enzymatic functioning of fatty acid desaturases. IPTG-induced fad3 cDNA expression in E. coli cells was suggested to be toxic to bacterial growth.

• Journal of Food Hygiene and Safety
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• v.15 no.3
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• pp.262-266
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• 2000

The polymerase chain reaction was used to selectively detect sequences within the fimbrial antigen of Salmonella enteritidis. Sterile milk was artificially inoculated with known amount of S. enteritidis and then DNA was extracted with guanidine thiocyanate/phenol/chloroform, followed by PCR. A detection limit of as few as 100 colony forming unit (cfu) per 0.5 ml milk was obtained with this method. For the whole procedure, it took only 5 h. A semi-quantitative polymerase chain reaction assay which allows an estimation of colony forming unit of S. enteritidis was developed. Known amount of standard plasmid pGem-4Z-Sef B(-) containing cloned S. enteritidis fimbrial antigen gene was co-amplified with Salmonella genomic DNA isolated from artificially inoculated milk. The same set of primers were used for the amplification and the products were cleaved with Bam HI. The concentration of the target DNA could be estimated by comparing the intensity of the two bands after electrophoresis. The PCR-based protocol described in this paper provides a rapid, simple, and sensitive method for detecting S. enteritidis in milk.

• Journal of The Korean Society of Agricultural Engineers
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• v.56 no.3
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• pp.19-29
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• 2014

This study was to evaluate the potential climate change impact on watershed hydrological components of evapotranspiration, surface runoff, lateral flow, return flow, and streamflow using Soil and Water Assessment Tool (SWAT). For Yongdam dam watershed (930 $km^2$), the SWAT model was calibrated for five years (2002-2006) and validated for three years (2004-2006) using daily streamflow data at three locations and daily soil moisture data at five locations. The Nash-Sutcliffe model efficiency (NSE) and coefficient of determination ($R^2$) were 0.43-0.67 and 0.48-0.70 for streamflow, and 0.16-0.65 and 0.27-0.76 for soil moisture, respectively. For future evaluation, the HadGEM3-RA climate data by Representative Concentration Pathway (RCP) 4.5 and 8.5 scenarios were adopted. The biased future data were corrected using 30 years (1982-2011, baseline period) of ground weather data. The HadGEM3-RA 2080s (2060-2099) temperature and precipitation showed increase of $+4.7^{\circ}C$ and +22.5 %, respectively based on the baseline data. The impacts of future climate change on the evapotranspiration, surface runoff, baseflow, and streamflow showed changes of +11.8 %, +36.8 %, +20.5 %, and +29.2 %, respectively. Overall, the future hydrologic results by RCP emission scenarios showed increase patterns due to the overall increase of future temperature and precipitation.

• Journal of Food Hygiene and Safety
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• v.22 no.4
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• pp.248-256
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• 2007

In this study, the competitive polymerase chain reaction (cPCR) was used to develop a direct enumeration method of Salmonella spp. in pork meat. After comparing three DNA extraction methods, the modified guanidine thiocyanate-phenol-chloroform method was chosen for Salmonella DNA extraction in artificially inoculated pork meat. The previously reported 284-bp invA gene (Rahn et al. Mol. Cell. Probes 1992) was tested for specificity, and 57 Salmonella strains and 24 non-Salmonella strains were evaluated. All Salmonella strains tested were invA positive, and all non-Salmonella strains produced no false positive amplification products. The detection limit achieved was as low as 1,460 colony-forming units (cfu) per 0.1g of pork meat. For cPCR, the invA gene, which features a 82 bp-deletion, was cloned in the pGEM-4Z vector. A known amount of competitor DNA, which has the same primer binding sites, was co-amplified with Salmonella chromosomal DNA from the artificially inoculated pork meat. The cell-number determined by cPCR was approximately equal to the cfu from the most probable number (MPN) method. Finally, the whole procedure took only 5 hr.

• The Korean Journal of Community Living Science
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• v.14 no.2
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• pp.83-92
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• 2003

This study was carried out to investigate the supplementary effects of soybean oil and rice gem oil compared with lard on lipid metabolism of insulin dependent diabetic mice. Streptozotocin-induced diabetic mice were fed three kinds of experimental diets with 20% lipid from lard(L), soy bean oil(SBO) and rice gem oil(RGO) for 7 weeks, respectively. Diet intake, body weight, organs weights and lipids levels of serum, liver and feces were measured. There was no significant difference in diet intake, body and organs weights among experimental groups. But the concentrations of serum triglyceride of SBO and RGO groups, and of serum total cholesterol were lower in RGO group than in the other groups. The hepatic total lipid and total cholesterol levels of RGO group were significantly lower than those of the other groups. The contents of total lipid and total cholesterol excreted in feces of SBO and RGO groups were higher than those of L group, and the significance was shown only in RGO group. These results suggested that soy bean oil and rice germ oil can reduce serum triglyceride and total cholesterol levels and hepatic total lipid concentration of insulin dependent diabetic mice compared with lard as a animal fat source by increasing fecal lipid excretion of these groups. But the significant reducing effects on serum and liver lipid levels were shown only in RGO group, and we need to investigate the hypolipidemic effect of this oil by supplementary level and period.

• Biomolecules & Therapeutics
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• v.8 no.2
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• pp.153-159
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• 2000

A novel serine/threonine protein phosphatase with EF-hand motif, which belongs to PPEF family was partially cloned from rat brain cDNA by employing RT-PCR method. The size of the amplified clone was 1.6kbp. The amplified DNA was subcloned into pGEM-T-Easy vector and the resulting plasmid was maned as pGEM-rPPEF2. The nucleuotide sequence is shared by 88% with that of mouse PPEF-2 cDNA, and the deduced amino acid sequence reveal 92% homology with that of mouse PPEF-2 cDNA. The N-terminal region of the cloned rat brain PPEF contains a putative phosphatase catalytic domain (PP domain) and the C-terminal region contains multiple $Ca^{2+}$ binding sites (EF region). The putative catalytic domin (PP) and the EF-hand motif (EF) regions were subcloned into pGEX4T-1 and were overexpressed in E. coli DH5 as glutathione-S-transferase (GST) fusion proteins. Expression of the desired fusion protein was identified by SDS-PAGE and also by immunoblot analysis using monoclonal antibody against GST. The recombinant proteins were purified by glutathione-agarose chromatography. This report is first to demonstrate the cloning of PPEF family from rat brain tissues. The clone reported here would be invaluable for the investigation of the role of this new type-phosphatase in rat brain.

• Research in Plant Disease
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• v.14 no.3
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• pp.223-225
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• 2008

This study was carried out to identify the Rice black-streaked dwarf virus that infected maize plants collected from Gochang-gun in Jeollabukdo in 2005. The genomic dsRNA from infected plants was extracted and the genome pattern was analyzed by polyacrylamide gel electrophoresis. Results of the electrophoresis revealed the already known to-segment genome and the difference of mobility was confirmed in isolates by collected areas. The RBSDV was identified from the result of RT-PCR using the template of extracted dsRNA and specific primer. The results of S10 cloned to pGEM-T vector and the conducted in sequence analysis consisted of 1,801nt and 559aa. This was of the same size as the RBSDV S10 identified in rice, and the change was confirmed in 18 base and displayed homology of 99%.

• Environmental Engineering Research
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• v.21 no.1
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• pp.84-90
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• 2016

Regionally the lowest average concentration of TOC was observed with 0.66 mg/L in Nakdong river, while the highest concentration of TOC was observed with 0.91 mg/L in Yeongsan river. The average concentration of TOC for national water quality monitoring site showed that the lowest average concentration of TOC was 1.58 mg/L in Han river, while the highest concentration of TOC was 3.37 mg/L in Yeongsan river. Seasonally, the average concentration of TOC at six upstream sites showed 0.77 mg/L and 0.56 mg/L, 0.69 mg/L and 0.63 mg/L, 0.80 mg/L and 0.73 mg/L, and 1.21 mg/L and 0.68 mg/L between wet season and dry season in Han river, Nakdong river, Gem river and Yeongsan river, respectively. For the national water quality site, the average concentration of TOC between wet season and dry season was 1.70 mg/L and 1.45 mg/L in Han river, 2.01 mg/L and 1.75 mg/L in Nakdong river, 2.01 mg/L and 1.60 mg/L in Gem river, and 3.75 mg/L and 3.00 mg/L in Yeongsan river. The distribution of TOC in upstream and national water quality monitoring sites on four major rivers have been influenced by seasonal and regional characteristics in Korea.

• Journal of the Mineralogical Society of Korea
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• v.20 no.2 s.52
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• pp.91-96
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• 2007

Notable characteristics are found between diamond types and observed optical properties from the analysis of natural diamonds in market as a gem mineral. All of the diamond samples observed are classified into type Ia, which can be subdivided type IaA containing only A aggregates, type IaB containing only B aggregates, and type IaAB containing both A aggregates and B aggregates in detail. As B aggregates more relatively increase than A aggregates. It is possible to find out that an increase of N3 center, an enhancement of blue fluorescence reaction, and an intensification of irregularity in the strain pattern. Because the property change of diamond mentioned above are consistent with optical phenomenon caused by dislocation and with N3 center produced by changes of nitrogen aggregation process from A aggregate to B aggregate. There is a close relation between diamond type and optical properties.