• BMB Reports
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• v.28 no.6
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• pp.538-545
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• 1995

When DNA replication of simian virus 40 (SV40) is initiated on the replication origin, the regions containing the initiation sites of DNA primase, which participates in the transient RNA primer synthesis for formation of Okazaki fragments in the lagging strand, were chosen as the target sites of antisense RNA for studies of the inhibition of SV40 DNA replication. Four recombinant transcription vectors, pUC-PrI, pUC-PrII, pGEM-PrBS, and pGEM-PrSN, coding antisense RNA, were constructed. Four antisense RNAs (named as I, II, BS, and SN) having the size of 18, 19,58, and 123 nts, respectively, were made from the transcription vectors by in vitro transcription. And then, antisense RNA in the concentration of 2${\mu}m$ were added to COS cells transfected with pATSV-W which is a recombinant plasmid containing the SV40 origin of replication. The inhibitory extent of DNA replication was measured by DpnI resistance and was confirmed by measurement of transient RNA primer synthesis. The result shows that six combinations of antisense RNA (I, II, BS, SN, I+SN, and BS+SN) lead to the inhibition of SV40 DNA replication by up to 85%.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.337-337
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• 2019

지구 온난화에 따른 기후변화로 인하여 태풍, 폭염, 홍수 및 가뭄 등과 같은 다양한 자연재해는 해마다 증가하고 있으며, 이에 따른 사회적 우려의 목소리가 커지고 있다. 특히 극한 강우와 홍수는 막대한 재산피해와 인명사고 등과 같은 재난에 직결된다. 자연재해에 대한 피해를 사전에 방지하기 위해서는 수자원 시스템을 이해하고, 미래 기후변화를 고려하는 것이 중요하다. 이미 많은 국가들은 기후변화에 대한 영향을 분석하고, 이에 적응하기 위한 노력을 하고 있다. 일반적으로 기후 모델로부터 생산된 모의자료를 이용하여 현재기간에 대비한 미래기간의 변화를 분석하게 되며, 이미 수문통계학 분야에서는 미래 강수량 변화를 살펴보기위해 다양한 연구가 수행되었다. 본 연구는 HadGEM3-RA 기후 모델의 강수 자료에서 연최대 자료를 추출하였고, 이를 이용하여 비정상성 지역빈도해석을 수행하였다. 지역빈도해석 방법은 홍수지수법(index flood method)을 이용하였고, 대상유역으로 한강유역을 선정하여 적용하였다. 또한 RCP(Representative Concentration Pathways) 시나리오는 RCP 4.5와 RCP 8.5를 적용하였으며, 각 시나리오에 따른 강수량 변화율은 전망 기간(S0:1979-2005, S1:2011-2040, S2: 2041-2070, S3:2071-2100)에 따라 비교 분석하였다.

• Journal of Astronomy and Space Sciences
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• v.40 no.1
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• pp.19-27
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• 2023

We present the results of using small telescopes in the United Arab Emirates (UAE) for observing variable stars and asteroids. Two telescopes, namely, 5" apochromatic refractor and 14" Schmidt-Cassegrain, at Al-Khatim Observatory (M44) were used for the observations. The targets were the three variable stars RR GEM, AG LMi, and DL CMi and the asteroid 22 Kalliope. We found a good consistency between our light curves and published ones for the calibration targets, i.e., the asteroid 22 Kalliope and the variable star RR GEM. According to previous studies, AG LMi has two suggested periods, 16.3 hours and 32.62 hours. Our results clearly confirm the second one, with a period of 32.6175 hours. The star DL CMi has several suggested periods as per previous studies, such as 4.0173 days, 1.9606 days, and 2.0086 days. Our observations confirm the first one, with a period of 4.0159 days. These results demonstrate the effectiveness of using small telescopes for observing variable stars and asteroids. This work provides some recommendations on using small telescopes for such observations.

• Journal of Microbiology and Biotechnology
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• v.34 no.4
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• pp.978-984
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• 2024

Genome-scale metabolic model (GEM) can be used to simulate cellular metabolic phenotypes under various environmental or genetic conditions. This study utilized the GEM to observe the internal metabolic fluxes of recombinant Escherichia coli producing gamma-aminobutyric acid (GABA). Recombinant E. coli was cultivated in a fermenter under three conditions: pH 7, pH 5, and additional succinic acids. External fluxes were calculated from cultivation results, and internal fluxes were calculated through flux optimization. Based on the internal flux analysis, glycolysis and pentose phosphate pathways were repressed under cultivation at pH 5, even though glutamate dehydrogenase increased GABA production. Notably, this repression was halted by adding succinic acid. Furthermore, proper sucA repression is a promising target for developing strains more capable of producing GABA.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.6B
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• pp.399-406
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• 2012

The objective of this research is to develop greenhouse gas generation models and estimation method of their parameters for solid waste landfills. Two models obtained by differentiating the Modified Gompertz and Logistic models were employed to evaluate two parameters of a first-order decay model, methane generation potential ($L_0$) and methane generation rate constant (k). The parameters were determined by the statistical comparison of predicted gas generation rate data using the two models and actual landfill gas collection data. The values of r-square obtained from regression analysis between two data showed that one model by differentiating the Modified Gompetz was 0.92 and the other model by differentiating the Logistic was 0.94. From this result, the estimation methods showed that $L_0$ and k values can be determined by regression analysis if landfill gas collection data are available. Also, new models based on two models obtained by differentiating the Modified Gompertz and Logistic models were developed to predict greenhouse gas generation from solid waste landfills that actual landfill generation data could not be available. They showed better prediction than LandGEM model. Frequency distribution of the ratio of Qcs (LFG collection system) to Q (prediction value) was used to evaluate the accuracy of the models. The new models showed higher accuracy than LandGEM model. Thus, it is concluded that the models developed in this research are suitable for the prediction of greenhouse gas generation from solid waste landfills.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.24 no.3
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• pp.99-105
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• 2014

$LiCoO_2$ thin film have received attention as cathodes of thin-film microbatteries. In this study, $LiCoO_2$ thin films were synthesized on Au substrates by sol-gel spin coating method and electrochemical properties were investigated under annealing temperature and time. The phycochemical properties of $LiCoO_2$ thin film were investigated by X-ray diffraction, scaning electron microscopy and atomic force microscopy. The electrochemical properties were characterized using galvanostatic charging/discharging cycling tests. From X-ray diffraction, as-grown films annealed at $550^{\circ}C$ and $750^{\circ}C$ are presumed to be spinel structure and a single phase of the layered-rock-salt, respectively. The RMS roughness and grain size of the films which annealed at $750^{\circ}C$ has similar values for annealing time 10 and 30 min, while for annealing time 120 min surface roughness, grain size increase and pore appearance were observed. The first discharge capacity of $LiCoO_2$ thin films annealed at $750^{\circ}C$ for 10, 30 and 120 min is about 54.5, 56.8 and $51.87{\mu}Ah/cm^2{\mu}m$, respectively. Corresponding capacity retention at 50th cycle is 97.25, 76.69, 77.19%.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.23 no.3
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• pp.124-128
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• 2013

In this research, cubic zirconia is synthesized with a refined CaO from shells as a stabilizer through Skull melting method. The proper process time and concentration are defined by Hydration reaction to produce the refined CaO after two different treatments using 0.1 mol% of HCl respectively with Cockle shell. The highest purity of CaO is reached when the shell is immersed in 1 mol% HCl. In Hydration reaction step, the pure $Ca(OH)_2$ is produced at $45^{\circ}C$ for 24 hours. The highest purity of CaO is measured when the $Ca(OH)_2$ is treated by heat at $1200^{\circ}C$ for 5 hours. The single crystals are grown through Skull melting method by adding the different contents of the refined CaO from 10 mol% to 30 mol% into $ZrO_2$. The frequency of High-frequency oscillator used for Skull melting method is 3.4 MHz. The descending speed of the single crystal is 3 mm/hour. The grown length of the single crystal is 4 cm. As a result of this study, 15 mol% of CaO has the best crystallinity.

• Applied Biological Chemistry
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• v.39 no.2
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• pp.112-117
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• 1996

Arginine decarboxylase (ADC) is the first enzyme in one of the two pathways of diamine putrescine biosynthesis in plants. The genes encoding ADC have previously been cloned from Escherichia coli, oat and tomato genome. Two degenerate oligonucleotides (17-mer) corresponding to two conserved regions of ADC were used as primers in polymerase chain reaction of rice (Oryza sativa L.) genomic DNA, and an approximately 1.0 kbp fragment was obtained. This amplified PCR product showed an open reading frame which contains 1,022 bp of nucleotide sequences. This PCR product was cloned into pGEM-originated T vector and the short 500 bp PstI digested fragment was subcloned into pGEM-3zf(+/-) vectors to facilitate sequencing. The nucleotide sequence of this PCR product showed about 74% and 70% identity with the same regions of the oat and tomato ADC cDNA sequences, respectively. The predicted amino acid sequence exhibited 45% and 62% identity with oat and tomato ADC polypeptide fragments, respectively. The sequence similarities of 34%, 47% and 38% were previously reported in oat and E. coli, tomato and oat, and tomato and E. coli ADC amino acids, respectively. Therefore, similarities and identities between rice and oat or tomato are remarkably higher than those others of the previous reports. In the highly conserved regions in both the amino acid sequence and spacing regions among the sequences of these three, rice ADC open reading frame also has the exactly same regions with the striking similarity. RNA blot analysis showed that hnc is expressed as a transcript of approximately 2.5 kbP in the rice seedling leaf tissues.

• Journal of Life Science
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• v.25 no.2
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• pp.158-163
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• 2015

Rice embryo is more abundant than endosperms in nutrients such as proteins, lipids, and vitamin B1. In this study, we constructed p53 plasmid that could be expressed in a plant system, and investigated optimal germination conditions in a variety of media. For construction of p53 plasmid, we performed p53 amplification from pCDNA-p53, subcloned to TA cloning vector, and then reconstructed into pGEM-CaMV plant expression vector. On the other hand, we prepared a variety of imbibition buffers and complete media for efficient germination of the rice embryo. Imbibition buffers prepared with different concentrations of salt or detergent showed no significant effect on germination efficiency. We prepared further culture media, such as solid agar, liquid media, and paper towel to establish the optimal conditions. Rice embryo showed germination rates of more than 70% in the solid medium, more than 60% in the paper towel medium, but less than 25% in liquid media, although germination rate did not differ with varying concentrations of salt and sucrose in culture media. Under the optimal germination conditions, we introduced the p53 plasmid using imbibition method, and finally detected human p53 gene expression in the germinated rice embryo. This method might present a novel, practical approach for evaluating efficient gene expression utilizing imbibition method in rice embryo.

• Proceedings of the Korean Society for Information Management Conference
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• 2002.08a
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• pp.109-114
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• 2002

더블린 코어는 15개의 기본 요소로 되어 있어서 매우 단순하지만 자관의 사정에 따라 확장사용이 가능하다. 본 연구에서는 교육분야의 메타데이터의 확장요소 사용을 위해서 DC Education, GEM, EdNA, EUN, 한국교육학술정보원 등 교육분야 다섯 개 시스템을 대상으로 확장요소를 비교·분석하여 여섯 개의 공통 추가 요소를 추출해서 제안하였다.