• 제목/요약/키워드: gel mobility

검색결과 151건 처리시간 0.023초

Polyacrylamide Gel Electrophoresis에 있어서 酸性 Mucopolysaccharide의 移動度의 變化 (Variation of the Electrophoretic Mobility of Acid Mucopolysaccharides in Polyacrylamide Gel Electrophoresis)

  • 김기창;줄리오 루도위-크
    • 대한화학회지
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    • 제18권2호
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    • pp.132-137
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    • 1974
  • 酸性 mucopolysaccharide는 鯨胎兒의 nucleus pulposus에서 分離하였다. Gel electophoresis에 있어서 酸性 mucopolysaccharide의 分離는 $0.03{\%}$의 hexamine cobaltic chloride 를 含有하는 0.05M 醋酸소-다 緩衝溶液(pH4.8)中에서 가장 좋았다. Spacer gel 上의 mucopolysaccharide 溶液을 $40{\%}$ sucrose 溶液으로 덮었을 때 mucopolysaccharide의 移動度에 變化가 나타나는 것을 觀察하였으며 이 효과는 醋酸소-다 緩衝溶液에 hexamine cobaltic chloride 을 加하면 消失하는 것을 觀察하였다.

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NMR Relaxometry of Water in Set Yogurt During Fermentation

  • Mok, Chul-Kyoon;Qi, Jinning;Chen, Paul;Ruan, Roger
    • Food Science and Biotechnology
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    • 제17권5호
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    • pp.895-898
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    • 2008
  • The mobility of water in set yogurt during fermentation was studied using nuclear magnetic resonance (NMR) relaxometry. The spin-spin relaxation was analyzed using a 2-fraction model, resulting in 2 spin-spin relaxation time constants $T_{21}$ and $T_{22}$. Both $T_{21}$ and $T_{22}$ exhibited rapid changes between 2 and 4 hr of fermentation, coinciding with the drop in pH and the rise in lactic acid bacteria count. The spin-lattice relaxation time $T_1$ increased over the fermentation period. Both $T_1$ and $T_2$ showed an increase in the mobility of water upon gel formation during fermentation. Water redistribution within the gel matrix due to casein aggregation and structure forming may be responsible for the changes in mobility.

양털, Gel Pad 및 Sponge의 욕창예방 및 치료효과에 관한 연구 (A Comparative Study on the Effect of Gel Pad, Sheepskin and Sponge on Prevention and Treatment of Decubitus Ulcers)

  • 이은옥;김매자
    • 대한간호학회지
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    • 제4권3호
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    • pp.93-104
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    • 1974
  • Regardless of a patient's diagnosis, the care of his skin has been a primary concern of nurses throughout the years. Nurses has washed, dried, and rubbed the skin and have changed his position so as to keep the patient's skin in good condition and to protect him from pressure sores. However, we still find many-patients suffering from the pressure sores which may take many mothers or years of treatment and cost a lot to be repaired. In view of expensive cost for treatment of the sores and low ratios of nursing staff per patient for intensive nursing care, prophylaxis of the bed sores with sufficient aids is extremely important both for patients and for nurses. Therefore, the unique purpose of this study was to determine whether sponge is good enough to treat and prevent the pressure sore as compared with gel pad and sheepskin. Sixty patients in the age range of 15 through 45 who were in unconscious status or have difficulty of mobility were chosen between January and March of 1974 at Seoul National University Hospital. They were randomly assigned to each treatment and observed on every 4th day with regard to level of consciousness, status of mobility, moisture or dryness of the bed, general skin condition, intake of nutrition, vital signs and intervals of position change in comparison with the changes of skin over the bony prominences. The study results were reviewed in a statistical method analysis of valiance-to obtain the following findings: 1. There was no significantly different changes of skin over the sanctum or the trochanter in each group using get pad, sheepskin or sponge. It means that we may substitute sponge for expensive gel pad or sheepskin. 2. There was no significant difference among mean changes of patient's skin in relation to sheepskin, gel pad and sponge. 3. There was no significant changes of patient's akin in relation to level of consciousness, status of mobility, intake of nutrition, general skin condition, temperature, blood pressure or interval of position change. 4. There were no meaningful interactions between each treatment and level of consciousness, moisture or dryness of the bed, intake of nutrition, general skin condition, temperature or blood pressure. 5. Sheepskin and gel pad had significantly better influence on patients with limited mobility than on patients with immobility, and sponge on patients with immobility on than patients with limited mobility, 6. Sheepskin and sponge had meaningfully totter influence on patients changing position at below 2-hour interval, gel pad at 2-4-hour interval, sheepskin at 4-6-hour interval, and three of them at above 6-hour interval.

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고분자전해질 연료전지용 Ti 분리판을 위한 고분자와 Ti Sol-Gel 탄소코팅의 비교 연구 (Comparison Study of Polymer and Ti Sol-Gel Carbon Coating on Ti for PEMFC Bipolar Plates)

  • 양원석;이재호;노희석;유주현;박철민;이수연;문성모
    • Corrosion Science and Technology
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    • 제22권6호
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    • pp.447-456
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    • 2023
  • In this work, we performed a comparative study examining two coatings on Ti Gr.1 for use in fuel cell bipolar plates. The coatings consisted of carbon black as the conductor along with acrylic polymer and Ti Sol-Gel binder as the binder. Ti Sol-Gel that had precipitated as TiO2 in areas impregnated between carbon black gaps, thereby acting as a binder for carbon black and serving as a polymer coating. Neither of the coatings peeled off during the 90° bend test to check formability. The contact resistance of the TiO2 coating was found to be lower than that of the polymer binder coating. Moreover, due to coating shrinkage (denser) that occurred during the heat treatment process, the TiO2 binder coating showed almost the same level of corrosion resistance, as measured by potentiostatic and EIS tests, despite being thinner than the polymer coating. However, both the polymer binder coating and the TiO2 binder coating had many pores and irregularities internally (around 10 ~ 100 nm) and on the surface (0.1 ~ 2 ㎛). We considered that these pores and irregularities contributed to the lower corrosion resistance.

Helical Periodicity of $(dT)_n{\cdot}(dA)_n{\cdot}(dT)_n$ Triple - Stranded DNA

  • Kim, Ki-Hyun;Koo, Hyeon-Sook
    • BMB Reports
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    • 제30권6호
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    • pp.426-430
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    • 1997
  • The helical periodicity of the triple-stranded $(dT)_n{\cdot}(dA)_n{\cdot}(dT)_n$ sequence was determined by measuring gel-mobilities of bent DNA fragments containing the sequence. In the bent DNA fragments, a $GA_{22}G$ $CT_{22}C$ sequence was located between two bent DNA loci composed of six $A_{6}{\cdot}T_{6}$ repeats. and the DNA length between the bent DNA loci was varied by 1 base pair over a full helical turn. The gel mobility of each bent DNA fragment reflected the overall extent of DNA bending and varied with the DNA length between the two bent loci. Mobilities of the bent DNA fragments in 5% polyacrylamide gel were measured after preincubating the DNA fragments both in the presence and absence of $CT_{22}C$ oligonucleotide. By comparing the bent DNA fragments containing an intermolecular triplex structure with those of a genuine duplex structure in the gel mobilities, the helical periodicity of the $T_n{\cdot}A_n{\cdot}T_n$ triplex DNA was determined to be $11.5({\pm}0.3)bp/turn$.

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두류 전분의 노화 및 저장 중 겔 특성 (Retrogradation of Legume Starches and Their Gel Properties during Storage)

  • 권미라;신말식;안승요
    • 한국식품과학회지
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    • 제25권6호
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    • pp.742-746
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    • 1993
  • DSC에 의한 노화양상에서 호화시킨 다음 냉각하여 3시간 후에 다시 가열하였을 때의 enthalpy를 서로 비교한 결과 강남콩의 enthalpy가 가장 크게 나타나 노화가 빨리 일어났다. 반면 동부나 녹두는 노화가 느리게 일어났다. Avrami 방정식을 이용하여 $4^{\circ}C$에서 저장한 1% 전분현탁액의 노화속도를 시험한 결과에서도 비슷한 경향을 보였다. 저장중 8% 전분겔의 수축현상으로부터 강남콩이 가장 빨리 수축되어 이장현상이 컸으며, 팥도 상당히 수축되었고, 비교적 동부와 녹두는 적게 수축되어 이장현상이 적었다. 또한 저장중 8% 전분겔의 water mobility behavior에서의 변화는 동부와 녹두의 경우 1일까지 거의 변화가 없다가 그 이후 서서히 감소되었으나 팥과 강남콩의 경우 처음에는 water mobility가 적어 겔이 빨리 형성되고 물의 결합정도가 크나 1일 동안 저장하였을 때 water mobility가 상당히 증가된 것으로 나타나 단시간내의 겔의 결합력이 크게 감소된 것으로 나타났다.

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Capillary Gel Electrophoretic Analysis of Cattle Breeds Based on Difference of DNA Mobility of Microsatellite Markers

  • Lee, Mi-Ji;Yoon, Du-Hak;Jeon, Jin-Tae;Eo, Seong-Kug;Kang, Seong-Ho
    • Bulletin of the Korean Chemical Society
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    • 제30권11호
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    • pp.2655-2660
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    • 2009
  • A breed of cattle, i.e., Korean cattle (Hanwoo), was identified based on the DNA mobilities of their microsatellites (MSs) by capillary gel electrophoresis (CGE) with a laser-induced fluorescence (LIF) detector. The MS markers were used for the accurate identification of species-specific genes. The DNA mobilities of the MS markers of Hanwoo and Holstein were measured using a CGE system with a fused-silica capillary (inner diameter of 75 ${\mu}m$, outer diameter of 365 ${\mu}m$, and total length of 50 cm). The capillary was dynamically coated with 1.0% (w/v) polyvinylpyrrolidone ($M_r$ = 1,000,000) and then filled with a mixture of 1.3% (w/v) poly(ethylene oxide) ($M_r$ = 600,000) and 1.9% (w/v) poly(ethylene oxide) (Mr = 8,000,000) as a sieving gel matrix. The species-specific genes of Hanwoo and Holstein were clearly distinguished within 33 min. This CGE assay technique is expected to be a useful analytical method for the fast and accurate identification of breeds of cattle.

Simple Route to High-performance and Solution-processed ZnO Thin Film Transistors Using Alkali Metal Doping

  • 김연상;박시윤;김경준;임건희
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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    • pp.187-187
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    • 2012
  • Solution-processed metal-alloy oxides such as indium zinc oxide (IZO), indium gallium zinc oxide (IGZO) has been extensively researched due to their high electron mobility, environmental stability, optical transparency, and solution-processibility. In spite of their excellent material properties, however, there remains a challenging problem for utilizing IZO or IGZO in electronic devices: the supply shortage of indium (In). The cost of indium is high, what is more, indium is becoming more expensive and scarce and thus strategically important. Therefore, developing an alternative route to improve carrier mobility of solution-processable ZnO is critical and essential. Here, we introduce a simple route to achieve high-performance and low-temperature solution-processed ZnO thin film transistors (TFTs) by employing alkali-metal doping such as Li, Na, K or Rb. Li-doped ZnO TFTs exhibited excellent device performance with a field-effect mobility of $7.3cm^2{\cdot}V-1{\cdot}s-1$ and an on/off current ratio of more than 107. Also, in case of higher drain voltage operation (VD=60V), the field effect mobility increased up to $11.45cm^2{\cdot}V-1{\cdot}s-1$. These all alkali metal doped ZnO TFTs were fabricated at maximum process temperature as low as $300^{\circ}C$. Moreover, low-voltage operating ZnO TFTs was fabricated with the ion gel gate dielectrics. The ultra high capacitance of the ion gel gate dielectrics allowed high on-current operation at low voltage. These devices also showed excellent operational stability.

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Bacillus circulans F-2가 생산하는 $\alpha$-Amylase에 관한 연구 (제 1보) $\alpha$-Amylase의 정제 (Studies on $\alpha$-Amylase of Bacillus circulans F-2 (Part I) Purification of $\alpha$-amylase)

  • 정만재
    • 한국미생물·생명공학회지
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    • 제9권4호
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    • pp.185-190
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    • 1981
  • 감자 생전분의 분해력이 강한 $\alpha$-amylase를 생산하는 Bacillus circulans F-2를 선발하고, 이 균주가 생산하는 $\alpha$-amylase를 정제하였으며, 정제효소의 polyacrylamide disc gel electrophoresis, SDS-polyacrylamide disc gel electrophoresis 및 soluble starch에 eo한 분해산물을 검사하고 그 결과를 요약하면 다음과 같다. 1 조효소액을 corn starch흡착, 유안분획, Bio-Gel P-100에 의한 gel filtration 및 DE-32 column chromatography에 의하여 specific activity 50.0 u/mg protein(원 비활성의 약 23배), 수율 25. 5%의 정제효소를 얻었다. 2. 정제효소에 대하여 polyacrylamide disc gel electrophoresis를 실시한 결과 $\alpha$-amylase activity를 가지는 아주 인접된 2ro의 Band가 나타났으나, SDS-polyacrylamide disc gel electrophoresis의 결과, polyacrylamide disc gel electrophoresis에서 나타난 2개의 Band는 charge가 약간 다른 charge isomer의 $\alpha$-amylase임을 시준하는 single band가 나타났다. 3. Polyacrylamide의 농도에 따른 2개 Band의 log mobility의 plot는 charge isomer를 가리키는 평행선을 나타내었다. 4. 두 효소단백질 Band의 작용 pattern을 알기 위하여 2개의 Band를 각각 분리하여 추출하고 soluble starch에 작용시켜 생성된 oligosaccharide의 pattern을 paper chromatography로 확인한 바 2개의 효소단백질 Band는 동일한 작용 pattern을 나타내었다. 5. Soluble starch로부터 생성되는 유일한 초기 가수분해산물은 maltohexaose이었다.

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Al-doped ZnO via Sol-Gel Spin-coating as a Transparent Conducting Thin Film

  • Nam, Gil-Mo;Kwon, Myoung-Seok
    • Journal of Information Display
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    • 제10권1호
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    • pp.24-27
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    • 2009
  • A simple nonalkoxide sol-gel route for depositing an Al-doped ZnO thin film on a glass substrate was derived in this study. The initial Al dopant concentration in the sol-gel preparation varied and ranged from 0 to 5%. The sol-gel-derived thin films showed c-plane preferred crystallization of their hexagonal phase, with nanosized grain structures. First and second post-heat-treatments were carried out to improve the film’s electrical resistivity. The carrier density and the Hall mobility were measured and discussed to explain the electrical resistivity. The optical transmittance within the visible range showed compatible properties, which indicates the possible use of A1-doped ZnO as a transparent electrode in flat panel displays.