• 한국응용과학기술학회지
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• 제7권2호
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• pp.71-73
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• 1990

Gas-liquid chromatography analyses have been carried out to investigate the fatty acid composition of 3 commercial Korean shortenings. Fourteen fatty acids ranged from caprylic to behenic acids were identified in all the samples. The major components in the samples were palmitic, stearic and oleic acids as the sum of 16:0, 18:0 and 18:1 ranged from 76-92% of total fatty acids. The ranges for saturated fatty acids, mono unsaturated fatty acids and polyunsaturated fatty acids in the samples were 49-50%, 41-42% and 4-6%, respectively.

• Bulletin of the Korean Chemical Society
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• 제26권4호
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• pp.575-578
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• 2005

Electrospray ionization mass spectrometry (ESI MS) was used, along with gas chromatography-mass spectrometry (GC-MS), to monitor Paal-Knorr synthesis of 2,5-dimethyl-1-phenylpyrrole by condensation of aniline with 2,5-hexanedione. In addition to 2,5-dimethyl-1-phenylpyrrole observed as a single spot by TLC, unexpected dimer size compounds were observed by GC-MS. Dimers and trimers were observed by ESI MS. ESI tandem mass spectrometry was used to select plausible structures for the dimer. ESI MS with or without liquid chromatographic separation is useful for observing oligomeric byproducts with low volatility produced in organic reactions.

• 생약학회지
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• 제21권3호
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• pp.235-238
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• 1990

Fatty acids, sterols and amino acids were confirmed from seeds of Acanthopanax senticosus for. inermis Harms. Fatty acids were isolated from petroleum ether extract and identified by gas liquid chromatography. Its composition was myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid and behenic acid. The composition of sterols that isolated from n-hexane extract were determined by GC/MS. It was confirmed to be a mixture of ${\beta}-sitosterol$ and stigmasterol. And several kinds of amino acids-cysteic acid, threonine, serine, proline, glutamic acid, glycine, alanine, valine, isoleucine, leucine, histidine, lysine and arginine-were confirmed from water fraction.

• Biomolecules & Therapeutics
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• 제1권1호
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• pp.109-120
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• 1993

Mechanisms for the hypocholesterolemic effetcs of $\beta$-glucan remain unclear. Rats were divided into 3 groups; normal control group, atherogenic group(oral administration of cholesterol 40 mg/kg/day and vitamin $D_2$320,000 IU/kg/day),${\beta}$-glucan treatment group(oral administration of atherogenic treatment and ${\beta}$-glucan 0.135 g/kg/day). The ${\beta}$-glucan treatment group showed moderate increases of serum lipids concentration compared with atherogenic group. In histopathological examination, aortas showed no critical lesions. The total fecal neutral sterols and bile acids excreted for 6 days was increased compared with both normal and atherogenic group. These result\ulcorner suggest that mechanisms for the hypocholesterolemic effect of ${\beta}$-glucan on rats were due to the inhibition of cholesterol absorption in the intestinal lumen and acceleration of cholesterol catabolism in the liver.

• 한국응용과학기술학회지
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• 제7권1호
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• pp.71-76
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• 1990

Transesterification reactions (methyl methacrylate with monoethanolamine, methyl methacrylate with n-butyl alcohol, dimethylphthalate with ethylene glycol, dimethyl phthalate with monoethanolamine) were kinetically investigated in the presense of various metal acetate catalysts at $110^{\circ}C$. The amount of reactants was measured by gas and liquid chromatography, and the reaction rates also measured from the amount of reaction products and reactants upon each catalyst. The transesterification reactions were carried out under the first order conditions respect to the concentration of reactants, respectively. The overall reaction order was 2nd, Maximum reaction rates were appeared at the range of 1.4 to 1.6 in electronegativity of metal ions and maximum catalytic activities were obserbed at the range of 1.5 to 1,8 in instability constant of metal acetates.

• Journal of Microbiology and Biotechnology
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• 제12권5호
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• pp.793-800
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• 2002

The mycolic acids and fatty acids of mycolic acid- containing bacteria in various types of fluids were analyzed using capillary gas chromatography and mass spectrometry. As model strains, Brevibacterium and Coryebacterium species, which have corynomycolic acids ill the range of $C_{32}C_{36}$ in the whole cell, were investigated. Optimized solvents extraction procedures for the mycolic acids and fatty acids from the culture fluids were: chloroform/methanol (1:2, v/v) as the first extraction solvents fur 4 h; and chlorofunuwater (1:1, v/v) as the second extraction solvents far 1 h. These conditions gave above 95% recovery yields fur mycolic acids from the culture fluids. The mycolic acid profile for the whole cells and the culture fluids were similar fur all the media tested. Thus, the procedure described here could be applied for the identification of mycolic acid-containing bacteria in fermentation broth or liquid from of foods.

• 약학회지
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• 제13권4호
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• pp.111-120
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• 1969

The determination of $\alpha$-pyrrolidone in ginseng extract by gas liquid chromatography (GLC) was established. $\alpha$-Pyrrolidone in extract was tentatively identified by comparison of the relative retention time of sample peak with those of reference compound and coinjection noted peak enhancement. The .alpha.-pyrrolidone peak elimination was demonstrated by formation of oxime when treated with excess of NH$_{2}$OH ${\cdot}$ HCl and NaOH, allowed to stand at room temperature for 5 minute. The elimination was also observed when samples were treated by the same procedure. The above method was proved to be useful for the determination of $\alpha$-pyrrolidone in ginseng extract and in ginseng extract mixed with 51 plant extracts.

• 미생물학회지
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• 제25권1호
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• pp.67-72
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• 1987

Cellular fatty acid composition of eight strains, indluding six strains of Comamonas terrigena, and two type strains of Pseudomonas acidovorans, and P. testosteroni was determined by gas-liquid chromatography. Almost the same composition was found in all the strains tested, and hexadecanoic acid, hexadecenoic acid, and octadecenoic acid were accounted more than 70% of total fatty acid. However, P. testosteroni differed from C. terrigena and P. acidovorans by the presence of comparatively large amonuts of 2-hydroxy-hexadecanoic acid, and C. terrigena contained three to eight times as much tetradecanoic acid in P. acidovorans and P. testosteroni. According to the similarity values calculated on the basis of fatty acid composition, C. terrigena strains were divided into three groups differentiated in the requirement of growth factors, and C. terrigena, P. acidovorans, and P. testosteroni strains occupied separate position each other in the dendrogram.

• 분석과학
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• 제19권1호
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• pp.103-106
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• 2006

A 35-year-old Perubian who suffered from grand mal seizures died in the aircraft on his way from the United States to Hongkong via Incheon international airport of Korea. While performing the autopsy, 115 packs made with double layer of transparent film and black plastic bag containing cocaine were found in the ileum and large intestine. Among of them, 3 packs were ruptured. To determine the concentration of cocaine and its metabolites, blood, urine, bile, liver, spleen, heart, kidney, brain and gastric contents were taken and toxicological analysis was performed. Gas chromatography/mass spectrometry (GC/MS) following liquid-phase extraction using chloroform:isopropanol (=9:1) and derivatization with bis(trimethylsilyl)-trifluoroacetamide (contains 1% trimethylchlorosilane) was performed. High levels of cocaine, benzoylecgonine (BE) and ecgonine methylester (EME) were found in the postmortem blood (0.96, 3.09 and $5.59{\mu}g/mL$) and urine (32.85, 145.35 and $53.17{\mu}g/mL$), respectively. Cocaine and its metabolites were also detected in all other biological specimen.

• 분석과학
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• 제26권1호
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• pp.73-79
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• 2013

자극성 액체의 주성분인 allyl isothiocyanate, capsaicin 및 dihydrocapsaicin을 기체크로마토그래피-질량분석기로 정량하는 방법에 대하여 연구하였다. 방호용인 목적이지만 스프레이 약제의 유해성과 품질관리는 또 다른 사회적 문제를 야기할 가능성이 있다. 이를 위해 시판 유통되고 있는 호신용 스프레이로부터 자극성 액체의 pH, 약제의 세척을 위한 세제의 용해정도, 기타 물질의 사용유무를 검토하였다. 마지막으로 자극성 성분으로 이용되는 allyl isothiocyanate, capsaicin 및 dihydrocapsaicin의 함량을 GC-MS로 정량하였다. 약제의 pH는 5.7 정도로 약산성이었고, 세제를 사용할 경우 약제의 유화되는 정도가 우수하였고, 실험에 사용된 액체에는 형광물질 및 유해 유기용매가 포함되지 않았다. 이전 연구 결과를 바탕으로 기체크로마토그래피-질량분석기를 이용하여 분석한 실제시료 3가지에 포함된 성분은 한 가지 제품에서는 allyl isothiocyanate 단일 성분[47,600 mg/kg (47.6%)]이었고, 나머지 두 제품은 capsaicin[228 mg/kg~368 mg/kg (1.14%~1.84%)]과 dihydrocapsaicin [224 mg/kg~414 mg/kg (1.12%~2.07%)] 혼합성분으로 구성되어 있었다.