• 한국환경보건학회지
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• 제8권2호
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• pp.57-65
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• 1982

Saccharides in Korean honey have been analyzed by traditional wet chemical methods and several chromatographic technics gas, paper, column and thin layer chromatography. A simple, rapid and versatile method for the separation and determination of saccharides via high performance liquid chromatography were used eluting with $H_2O : CH_CN$ at 25:75, at a flow rate of 1.0ml/min. Acasia honey contained 36.8% fructose, 31.4% glucose, 1.7% sucrose and bush clover honey held 34.4% fructose, 32.3% glucose and 30% sucrose. Difference on the regional distribution were not found in the quantity of saccharides (42 species). The quality of inferior honey was assumed to contain much maltose, sugar and glucose than common honey.

• Food Science and Biotechnology
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• 제14권5호
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• pp.656-660
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• 2005

The relationship between the gas chromatographic (GC) patterns of sauce made of barley bran and ranked order in sensory analysis was investigated by multiple regression analysis (MRA). Most of the 42 barley bran sauce samples comprised about 34 peaks, in which the content of 9, 12-octadecanoic acid methyl ester was the highest, followed by those of 2-furanmethanol and 2-furancarboxaldehyde. It is difficult to estimate the aroma quality of barley bran sauce samples on the basis of only one peak. The 34 aroma compounds of the 42 samples were analyzed by an MRA model featuring six transformations. The most precise fit was calculated from the absolute value transformed with the root square of each peak, and the multiple determination coefficient showed that 91.6% of the variation in the sensory score could be explained on the basis of GC data.

• 한국대기환경학회지
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• 제18권6호
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• pp.475-485
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• 2002

An analytical method was investigated for the meaiiurement of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) concentrations in air samples. Procedures required for column chromatographic clean up. silicagel (stage I) and gel permeation chromatography (stage II), were discussed. Identification and quantification of PCBs and OCPs were performed using a combination of gas chromatography/mass spectrometry/ selected ion monitoring. Recovery tests calculated from six samples are 68∼137% for PCBs and 58∼130% for OCPs except for endrin aldehyde. Instrumental detection limits determined for the PCBs and OCPs varied from 0.05 to 0.18 pg/m3 and from 0.71 to 16.82 pg/㎥, respectively. The method has been applied to the analysis of air samples collected at Ansung city, Kyonggi province, Korea. This method may serve as a screening protocol for the simultaneous determination of PCBs and OCPs in air.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1996년도 한국생물과학협회 국제학술대회
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• pp.195.2-195
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• 1996

acs gene cloning was constructed by subcloning the 2.2-kb MunI-MunI restriction fragment of 638 and 639 which include acs gene from the kohara phage into the unique EcoRI site of pUC18 and pJM9131 containing the PHA biosynthesis genes. Then recombinant E. coli fadRatoC(Con) mutants containing the polyhydroxyalkanoate(PHA) biosynthesis genes are able to incoporate s significant levels of 3-hydroxyvalerate (3HV) into the copolymer [P(3HB-co-3HV)]. Quantitative determination of PHB and P(3HB-co-3HV) was performed by gas-chromatographic analysis of extracts obtained from methanolysis of lyophilized cells.

• Bulletin of the Korean Chemical Society
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• 제24권5호
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• pp.555-558
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• 2003

An enrichment/separation system for atomic absorption spectrometric determinations of Cu(Ⅱ), Fe(Ⅲ), Ni(Ⅱ) and Co(Ⅱ) has been established. The procedure is based on the adsorption of the analytes as calmagite chelates on Chromosorb-102. The effects of some parameters including pH, amount of ligand, salt matrix, flow rates of sample and eluent solutions were investigated. Under optimized conditions, the relative standard deviation of the combined method of sample treatment, preconcentration and determination with FAAS (N=5) is generally lower than 5%. The limit of detection (3σ) was between 6.0-112.9 ㎍/L. The results were used for preconcentration of analytes from some sodium and ammonium salt.

• Bulletin of the Korean Chemical Society
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• 제19권6호
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• pp.617-618
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• 1998

A simple and reproducible pretreatment method was developed for the determination of dioxins in milk sample. Liquid-liquid extraction (LLE) was used for the initial extraction of the analyte from milk. For the elimination of interferences coextracted from milk, acid treatment followed by multilayer silica gel, and then alumina column clean-up were performed. The clean extract could be obtained without carbon column or high performance liquid chromatographic (HPLC) clean-up procedure. Polychlorinated biphenyles (PCBs) and dioxins were separated on neutral alumina activated at 180 ℃ for 12 hours. The final extract was analyzed by HPLC and high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS). The recovery of dioxins spiked in milk at 75-300 ppt level was 83.3-98.9% and their relative standard deviation was 4.1-14%.

• Bulletin of the Korean Chemical Society
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• 제19권6호
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• pp.619-624
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• 1998

A simple and reproducible pretreatment method was developed for the determination of dioxins in milk sample. Liquid-liquid extraction (LLE) was used for the initial extraction of the analyte from milk. For the elimination of interferences coextracted from milk, acid treatment followed by multilayer silica gel, and then alumina column clean-up were performed. The clean extract could be obtained without carbon column or high performance liquid chromatographic (HPLC) clean-up procedure. Polychlorinated biphenyles (PCBS) and dioxins were separated on neutral alumina activated at 180 ℃ for 12 hours. The final extract was analyzed by HPLC and high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS). The recovery of dioxins spiked in milk at 75-300 ppt level was 83.3-98.9% and their relative standard deviation was 4.1-14%.

• Archives of Pharmacal Research
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• 제13권3호
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• pp.282-284
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• 1990

By saponification and extraction of corn oil foots abandoned as waste during oil refining, a mixture of phytosterols was obtained, and its major components were determined as .betha.-sitosterol, campesterol and stigmasterol by gas chromatographic analysis. The mixture is very cheap and regarded as an excellent substrate for direct fermentation of C-17 keto steroid intermediate for various steroid pharmaceuticals.

• 대한화학회지
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• 제30권5호
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• pp.465-474
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• 1986

농작물에 잔류되어 있는 11종의 유기인제 농약들을 추출하여 기체-액체 크로마토그래피로 동시에 분석함에 있어서 농작물별로 방해하는 다른 성분들을 분리, 제거하기 위한 액체-액체 분배와 컬럼크로마토그래피의 최적 조건을 찾고자 하였다. Acetone을 사용하여 농작물로부터 녹여낸 추출물에 포화 NaCl용액을 가하고 petroleum ether로 두 번 분배하여 농약들을 회수하였다. Activated carbon, magnesia 및 diatomaceous earth의 혼합 흡착제(1:2:4)를 충진시킨 컬럼에 petroleum ether에 분배된 추출물들을 넣고 methyl chloride로 용리시켰더니 가해준 농약의 82∼105%가 회수되었고 농작물에서 녹아나온 방해 성분들이 거의 제거되었다.

• 분석과학
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• 제6권3호
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• pp.283-288
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• 1993

기체 크로마토그래피를 이용하여 과수 재배용 살균제인 nuarimol의 분석을 위한 최적 조건을 조사하고 사과 중에서의 잔류성을 규명하였다. 사과시료를 methanol로 추출, 여과한 후 4.00% NaCl과 dicholromethane액으로 분리농축하고, alumina 컬럼상에서 1-chlorobutane과 methanol 혼합액으로 정제하여 GLC/ECD로 분석하였다. 0.200와 1.00ppm 표준물 첨가 실험결과 평균 회수율이 79~95%였고, 사과 중 nuarimol의 잔류량은 수확 3일 전 8회 처리시 0.0830ppm이었다. 사과에 대한 안전 사용기간은 수확 7일전 6회 사용이 적당한 것으로 나타났다.