• Title/Summary/Keyword: gas laser

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Study on the Contamination of Pseudomonas tolaasii in Oyster Mushroom (느타리버섯에서의 Pseudomonas tolaasii오염 연구)

  • 이혜영;장금일;김광엽
    • Journal of Food Hygiene and Safety
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    • v.16 no.3
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    • pp.232-240
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    • 2001
  • One hundred twenty five bacterial isolates were obtained from the brown blotch-diseased oyster mushrooms collected from markets. Among them, 45 were determined as pathogenic bacteria and white line forming organisms(WLFO) were 6 strains and white line reaction organisms (WLRO) were 6 strains. All of the white line forming isolates were identified as Pseudomonas tolaasii which is a known pathogen of brown blotch disease of oyster mushroom by GC-MIS(Gas chromatography-microbial identification system). Six of the white line reacting organisms were identified as P. chlomraphis, P. fluorescens biotype A and type C. The rest of them were P gingeri, P. agarici, P. fluorescens biotype B, P. chloroyaphis, non-pathogenic P. tolaasii, P. putida biotype A and B etc. For spectrum of activity of tolaasin, culture filtrates from pathogenic isolates were examined by browning of mushroom tissue and pitting of mushroom caps. The weak pathogenic bacteria didn't induce browning or pitting of mushroom tissue. On the other hand, strong pathogenic isolates showed browning and pitting reaction on mushroom. An extracellular toxin produced by P. tolaasii, was investigated. The hemolysis activity test of 6 strains identified as P. tolaasii were 0.8∼0.9 at 600 nm and 3 strains of WLRO were 0.9∼1.0 and Pseudomonas app. were 1.0∼1.2. Observation of fresh mushroom tissue using confocal laser scanning microscopy was carried out for images of optical sectioning and vertical sectioning. Also images of brown blotch diseased oyster mushroom tissue after contamination P. tolaasii was obtained by CLSM.

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Characteristics of a Wet Air Cleaning System for Removal of Air Pollutants (습식 공기청정장치의 공기오염물질 제거 특성)

  • Bae, Gwi-Nam;Kim, Yong Pyo;Baik, Nam Jun
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.1
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    • pp.21-31
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    • 2000
  • Performance characteristics of a wet air cleaning system were investigated experimentally, and discussed in relation with physicochemical properties of the target pollutants. The system is composed of an air cleaner, a separator, and a medium filter. Removal efficiency of the system was measured for ambient particles and gaseous air pollutants: $SO_2$, NO, $NO_2$, HCHO, and $NH_3$. For particle removal test, particles were introduced into the system through a fan, and the particle size distribution was measured at three locations by using two laser particle counters. Particle removal efficiency for each system component was obtained from the particle size distribution. It was found that the separator primarily removed coarse particles greater than $5{\mu}m$ in diameter, and that the medium filter mainly removed fine particles less than $5{\mu}m$ in diameter. For gas removal test, air with gaseous air pollutant was injected into the outlet of the fan, and the concentration was measured both at the upstream of the air cleaner and at the downstream of the separator. It was found that the gaseous species with high Henry's law coefficients, such as $SO_2$, HCHO, and $NH_3$, showed high removal efficiency, but the gaseous species with low Henry's law coefficients, such as NO and $NO_2$, showed low removal efficiencies. It was also found that negative ions were generated from the air cleaner.

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Growth of vertically aligned carbon nanotubes on Co-Ni alloy metal (Co-Ni 합금위에서 수직방향으로 정렬된 탄소나노튜브의 성장)

  • Lee, Cheol-Jin;Kim, Dae-Woon;Lee, Tae-Jae;Park, Jeong-Hoon;Son, Kwon-Hee;Lyu, Seung-Chul;Song, Hong-Ki;Choi, Young-Chul;Lee, Young-Hee
    • Proceedings of the KIEE Conference
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    • 1999.07d
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    • pp.1504-1507
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    • 1999
  • We have grown vertically aligned carbon nanotubes in a large area of Co-Ni codeposited Si substrates by the thermal CVD using $C_2H_2$ gas. Since the discovery of carbon nanotubes, Synthesis of carbon nanotubes for mass production has been achieved by several methods such as laser vaporization arc discharge, and pyrolysis. In particular, growth of vertically aligned nanotubes is of technological importance for applications to FED. Recently, vertically aligned carbon nanotubes have been grown on glass by PECVD Aligned carbon nanotubes can be also grown on mesoporous silica and Fe patterned porous silicon using CVD. Despite such breakthroughs in the growth, the growth mechanism of the alignment are still far from being clearly understood. Furthermore, FED has not been clearly demonstrated yet at a practical level. Here, we demonstrate that carbon nanotubes can be vertically aligned on catalyzed Si substrate when the domain density reaches a certain value. We suggest that steric hindrance between nanotubes at an initial stage of the growth forces nanotubes to align vertically and then nanotubes are further grown by the cap growth mechanism.

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Synthesis and Characterization of SnO2 Thin Films Deposited by Plasma Enhanced Atomic Layer Deposition Using SnCl4 Precursor and Oxygen Plasma

  • Lee, Dong-Gwon;Kim, Da-Yeong;Gwon, Se-Hun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.254-254
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    • 2016
  • Tin dioxide (SnO2) thin film is one of the most important n-type semiconducting materials having a high transparency and chemical stability. Due to their favorable properties, it has been widely used as a base materials in the transparent conducting substrates, gas sensors, and other various electronic applications. Up to now, SnO2 thin film has been extensively studied by a various deposition techniques such as RF magnetron sputtering, sol-gel process, a solution process, pulsed laser deposition (PLD), chemical vapor deposition (CVD), and atomic layer deposition (ALD) [1-6]. Among them, ALD or plasma-enhanced ALD (PEALD) has recently been focused in diverse applications due to its inherent capability for nanotechnologies. SnO2 thin films can be prepared by ALD or PEALD using halide precursors or using various metal-organic (MO) precursors. In the literature, there are many reports on the ALD and PEALD processes for depositing SnO2 thin films using MO precursors [7-8]. However, only ALD-SnO2 processes has been reported for halide precursors and PEALD-SnO2 process has not been reported yet. Herein, therefore, we report the first PEALD process of SnO2 thin films using SnCl4 and oxygen plasma. In this work, the growth kinetics of PEALD-SnO2 as well as their physical and chemical properties were systemically investigated. Moreover, some promising applications of this process will be shown at the end of presentation.

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Development of Single-Frame PIV Velocity Field Measurement Technique Using a High Resolution CCD Camera (고해상도 CCD카메라를 이용한 Single-Frame PIV 속도장 측정기법 개발)

  • Lee, Sang-Joon;Shin, Dae-Sig
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.24 no.1
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    • pp.21-28
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    • 2000
  • Although commercial PIV systems have been widely used for the non-intrusive velocity field measurement of fluid flows, they are still under development and have considerable room for improvement. In this study, a single-frame double-exposure PIV system using a high-resolution CCD camera was developed. A pulsed Nd:Yag laser and high-resolution CCD camera were synchronized by a home-made control circuit. In order to resolve the directional ambiguity problem encountered in the single-frame PIV technique, the second particle image was genuinely shifted in the CCD sensor array during the time interval dt. The velocity vector field was determined by calculating the displacement vector at each interrogation window using cross-correlation with 50% overlapping. In order to check the effect of spatial resolution of CCD camera on the accuracy of PIV velocity field measurement, the developed PIV system with three different resolution modes of the CCD camera (512 ${\times}$ 512, lK ${\times}$ IK, 2K ${\times}$ 2K) was applied to a turbulent flow which simulate the Zn plating process of a steel strip. The experimental model consists of a snout and a moving belt. Aluminum flakes about $1{\mu}m$ diameter were used as scattering particles for the liquid flow in the zinc pot and the gas flow above the zinc surface was seeded with atomized olive oil with an average diameter of 1-$3{\mu}m$. Velocity field measurements were carried out at the strip speed $V_s$=1.0 m/s. The 2K ${\times}$ 2K high-resolution PIV technique was significantly superior compared to the smaller pixel resolution PIV system. For the cases of 512 ${\times}$ 512 and 1K ${\times}$ 1K pixel resolution PIV system, it was difficult to get accurate flow structure of viscous flow near the wall and small vortex structure in the region of large velocity gradient.

The Spray Characteristics and Spray Behavior Characteristic in Exhaust Gas Flow of Urea Solution Injector (Urea 수용액 분사용 인젝터의 분무 특성과 배기관내 분무 거동 특성)

  • Oh, Jung-Mo;Han, Young-Deok;Kim, Ki-Bum;Lee, Ki-Hyung
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.34 no.11
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    • pp.999-1004
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    • 2010
  • Recently, many technologies have been developed in order to satisfy stringent emission regulations. However, in the case of diesel engines, the stringent emission regulations with respect to NOx and PM have not yet been satisfied. A dramatic reduction in the NOx and PM emissions could be achieved by using after-treatment systems such as lean NOx trap (LNT) and urea-SCR systems. However, the high temperature in the exhaust pipe affects the spray behavior of the secondary injector, which is used for supplying the Urea-SCR. Because of this high temperature, it is difficult to achieve uniform distribution of the reducing agent in the manifold. In this paper, the characteristics of a urea-SCR injector used for injecting in the exhaust pipe are presented. The purpose of this study was to investigate the spray characteristics of the injector, such as the spray angle, injection quantity, and SMD. In addition, laser diagnostics and high-speed-camera images were used to analyze the injector spray characteristics and to present a distribution of reduction in the transparent manifold.

Structural and Molecular Characterization of Extracellular Polysaccharides Produced by a New Fungal Strain, Trichoderma erinaceum DG-312

  • JOO JI-HOON;YUN JONG-WON
    • Journal of Microbiology and Biotechnology
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    • v.15 no.6
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    • pp.1250-1257
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    • 2005
  • Two groups of exopolysaccharides (designated as Fr-I EPS and Fr-II EPS) were isolated from the culture filtrate of new fungal strain Trichoderma erinaceum DG-312 by Sepharose CL-6B chromatography. The structures of the exopolysaccharides were investigated using gas chromatography (GC), Fourier transform-infrared (FT-IR) spectroscopy, GCMS analysis, and NMR. GC analysis indicated that Fr-I EPS was composed of mainly mannose ($78.9\%$) and galactose ($21.1\%$), whereas Fr-II EPS contained mannose ($68.4\%$), galactose ($26.2\%$), and glucose ($5.4\%$). In the anomeric region ($950-700cm_{-1}$) of the FT-IR spectrum, both EPSs exhibited obvious characteristic absorption of $810\;cm_{-1}$, indicating the existence of mannose. The spectra of $\alpha-and\;\beta$-configurations were assigned at 880 and $914\;cm_{-1}$, respectively. The results of GC-MS analyses confirmed that both EPSs were complex heteropolysaccharides with a ($1{\rightarrow}3$)-linked mannan backbone. The C-1 region that appeared in the $^{13}C-NMR$ spectra of these EPSs indicated a typical anomeric carbon signal. The Fr-I EPS showed two anomeric carbon signals at 102.6 and 99.6 ppm, whereas the Fr-II EPS displayed four anomeric carbon signals at 102.5, 99.6, 98.5, and 94.3 ppm. The molecular characteristics of the EPSs were further investigated using a size exclusion chromatography/multi-angle laser light scattering (SEC/MALLS) system. The SEC/MALLS system revealed that the average molar masses of the EPSs were $6.592{\times}10^{4}$ (Fr-I EPS) and $1.920{\times}10^{4}$ (Fr-II EPS) g/mol, and the molecular conformation of both EPSs in aqueous solution was random coils.

Effect of the Hydrogen Annealing on the Pb(Zr0.52Ti0.48)O3 Film using (Pb0.72La0.28)Ti0.94O3 Buffers ((Pb0.72La0.28)Ti0.94O3 Buffer를 사용한 Pb(Zr0.52Ti0.48)O3 박막의 수소 후열처리 효과)

  • Lee, Eun-Sun;Li, Dong-Hua;Chung, Hyun-Woo;Lim, Sung-Hoon;Lee, Sang-Yeol
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.18 no.4
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    • pp.327-329
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    • 2005
  • Pb(Zr/sub 0.52/Ti/sub 0.48/)O₃(PZT) thin films were deposited by using a pulsed laser deposition method on a Pt/Ti/SiO₂/Si substrate with (Pb/sub 0.72/La/sub 0.28/)Ti/sub 0.93/O₃ (PLT) buffer and on a Pt/Ti/SiO₂/Si substrate without buffer. These films were annealed in H₂-contained ambient for 30 minutes at the substrate temperature of 400。C to evaluate the forming gas annealing effects. The comparative studies on the ferroelectric properties of these two films were carried out, which are shown that ferroelectric properties, such as remanent polarization didn't change in the case of PLT buffered PZT film while remanent polarization value of PZT film degraded from 20.8 C/㎠ to 7.3 C/㎠. The leakage current became higher in both cases, but that of the more-oriented PZT film had the moderate value of the 10/sup -6/ order of A/㎠. This is mainly because the hydrogen atoms which make the degradation of PZT films cannot infiltrate into the more -oriented PZT film as well as the less-oriented PZT film.

A Case Study on the Framework Development of the Metal 3D Printing Control & Monitoring System (금속 3D프린팅 통합 제어 및 모니터링 시스템 개발을 위한 프레임워크에 관한 연구)

  • Jeon, Byung-Ju;Lee, Sun-Kyu;Lee, Seung-Hee;Jang, Sung-Ho;Jung, Goo-sang
    • Journal of Digital Convergence
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    • v.18 no.11
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    • pp.187-194
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    • 2020
  • This study present to Framework & R&D direction of the 3d printing Integrated Control & Monitoring System. To ensure this purpose, we developed integrated 3d printing control system Framework for DED & PBF and we introduce 4 monitoring system include photo diode, gas flow, acoustic and spectrometer sensors. For this study, we utilize metal 3d printing system from Conception., OKE Tech and DE&T who are still developing Metal 3D Printing Technology since 2017. In the result, we represent the latest 3D Printing Control and Monitoring System for the next 3D Printing researcher and we hope this study will be used as a basic reference and data for Cooperation between mechanic, electronic and material fields.

Experimental and Computational Studies on Particle Behavior in High Temperature Gas with the Various Temperatures of a Solid Wall (고체의 벽면온도에 따른 고온가스 내의 입자거동에 대한 실험 및 수치해석 연구)

  • Choi, Jae-Hyuk;Lee, Ki-Young;Yoon, Doo-Ho;Yoon, Seok-Hun;Choi, Hyun-Kue;Choi, Soon-Ho
    • Journal of Advanced Marine Engineering and Technology
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    • v.30 no.3
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    • pp.403-412
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    • 2006
  • The effect of a wall temperature on the soot deposition process from a diffusion flame to a solid wall was investigated in a microgravity environment to attain in-situ observations of the process. The fuel for the flames was an ethylene ($C_2H_4$). The surrounding oxygen concentration was 35% with surrounding air temperatures of $T_a=600K$. In the study, three different wall temperatures. $T_w$=300, 600, 800K, were selected as major test conditions. Laser extinction was adopted to determine the soot volume fraction distribution between the flame and burner wall. The experimental results showed that the maximum soot volume fractions at $T_w$=300, 800 K were $8.8{\times}10^{-6},\;9.2{\times}10^{-6}$, respectively. However, amount of soot deposition on wall surface was decreased because of lower temperature gradient near the wall with increasing wall temperature. A numerical simulation was also performed to understand the motion of soot particles in the flame and the characteristics of the soot deposition to the wall. The results from the numerical simulation successfully predicted the differences in the motion of soot particles by different wall temperature near the burner surface and are in good agreement with observed soot behavior that is, the 'soot line', in microgravity.