• Title/Summary/Keyword: gas chromatopraphy

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Quantification of 2-Acetyl-1-pyrroline from the Aroma Rice Germplasm by Gas Chromatography (Gas chromatography를 이용한 향미 유전자원의 2-acetyl-1-pyrroline 정량분석)

  • Kim, Jeong-Soon;Park, One-Sung;Ahn, Sang-Nag;Lee, Jung-Ro;Gwag, Jae-Gyun;Kim, Tae-San;Lee, Sok-Young
    • Korean Journal of Food Science and Technology
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    • v.40 no.5
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    • pp.516-521
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    • 2008
  • This study was conducted to optimize the analysis condition and quantify the 2-acetyl-1-pyrroline (2AP) in the brown aroma rice. Extraction effect of the solvent for 2AP was the order of ethanol>acetonitrile>methanol in the range from 30 to $90^{\circ}C$. In the extraction time of 15, 30, 60, and 90 min, the 30 min had the highest 2AP concentration, and it was decreased according to lapse of time. At grinding time, 5 sec resulted in highest 2AP concentration. It was recommended that five sec grinding time, using ethanol, at $90^{\circ}C$ for 30 min was the optimization conditions to quantify the 2AP. Hyangmibyeo2ho and Aranghyangchalbyeo were mild aroma. In the foreign aroma rice, 11 of 19 accessions of Indica types and 2 of 6 accessions of Japonica types were more than mild aroma. Finally, 30 accessions of aroma rice were selected based on their 2AP concentration and agronomic traits.