• 한국임상수의학회지
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• 제41권1호
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• pp.65-70
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• 2024

Ten cutaneous masses from Djungarina hamsters (Phodopus sungorus) were diagnosed as nine atypical fibromas and one fibrosarcoma derived from cutaneous ganglion cell-like cells. Among these patients, nine were male and one was female. Histologically, these abnormal masses were composed of spindle-shaped or polygonal neoplastic 'ganglion cell-like' cells with abundant amphophilic vacuolated cytoplasm. Immunohistochemically, these neoplastic cells were stained for vimentin, S100, and neuron-specific enolase. Out of 9 males, 3 showed positive reactions to the androgen receptor. This report provides a detailed histologic and immunohistochemical characterization of atypical fibroma, fibrosarcoma, and the tumorigenesis of ganglion cell-like cells in Djungarian hamsters.

• Clinical and Experimental Otorhinolaryngology
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• 제11권4호
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• pp.224-232
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• 2018

Objectives. Spiral ganglion neurons (SGNs) include potential endogenous progenitor populations for the regeneration of the peripheral auditory system. However, whether these populations are present in adult mice is largely unknown. We examined the presence and characteristics of SGN-neural stem cells (NSCs) in mice as a function of age. Methods. The expression of Nestin and Ki67 was examined in sequentially dissected cochlear modiolar tissues from mice of different ages (from postnatal day to 24 weeks) and the sphere-forming populations from the SGNs were isolated and differentiated into different cell types. Results. There were significant decreases in Nestin and Ki67 double-positive mitotic progenitor cells in vivo with increasing mouse age. The SGNs formed spheres exhibiting self-renewing activity and multipotent capacity, which were seen in NSCs and were capable of differentiating into neuron and glial cell types. The SGN spheres derived from mice at an early age (postnatal day or 2 weeks) contained more mitotic stem cells than those from mice at a late age. Conclusion. Our findings showed the presence of self-renewing and proliferative subtypes of SGN-NSCs which might serve as a promising source for the regeneration of auditory neurons even in adult mice.

• 대한세포병리학회지
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• 제4권1호
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• pp.52-56
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• 1993

Proliferative fasciitis is a benign pseudosarcomatous mesenchymal lesion occurring in the subcutis. The lesion occurs clinically as a tumorous mass that develops within a rather short time in elderly patients. We recently experiecened a case of fine needle aspiration cytology of proliferative fasciitis in the left anterior chest wall of a 72 year-old male patient. The smear revealed two types of cells. One was the large and mostly oval cell with one or two nuclei lying at the periphery of the cell body and abundant basophilic cytoplasm like the ganglion cell. The nuclei were round to oval, had vesicular chromatin and contained prominent nucleoli. The other was the spindle shaped fibroblast with an oval nucleus. The differential diagnosis includes a true tumor such as ganglioneuroma, rhabdomyosarcoma, and liposarcoma and therefore fine needle aspiration cytology is very much indicated in order to exclude these possibilities.

• 한국패류학회지
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• 제16권1_2호
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• pp.1-9
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• 2000

아프리카왕달팽이 Achatina fulica의 내장신경 절과 우체벽신경절은 좌, 우 양반구로 구성된 나비모습을 하고 있으며, 이들 신경절의 피질부와 수질부의 표피부위에는 신경세포가 밀집되어 있는 반면 중앙부위에는 신경섬유가 망상형으로 신경망을 구성하고 있었다. 두 신경절의 피질부 및 수질부에 위치한 신경세포들은 초대형신경세포(200 $\mu\textrm{m}$ 이상)와 대형신경세포(직경 60-70 $\mu\textrm{m}$ 이상), 중형신경세포(직경 30-40 $\mu\textrm{m}$) 그리고 소형신경세포(직경 10-15 $\mu\textrm{m}$) 등 4종류로 구분할 수 있었다. 초대형 및 대형신경세포는 20-22개 정도로 매우 소수가 관찰된 반면, 중형신경세포(약 400-500개)와 소형신경세포(약 700-800개)는 다수가 관찰되었다. AB/AY 이중염색반응에서 초대형 신경세포는 light Yellow cell(LYC)로, 대형 및 중형신경세포는 yellow green cell (YGC)과 dark green cell(DGC) 등 두 종류로 그리고 소형신경세포는 yellow cell(YC)과 blue cell(BC) 등으로 각각 확인되었다. Somatostatin 면역염색반응에서 양성반응을 나타낸 DGC는 성장조절물질 분비의 억제에 관여하는 것으로 확인되었으며, 초대형 및 대형신경세포는 신경분비기능 이외 포식작용을 수행하는 것으로 각각 확인되었다.

• 대한족부족관절학회지
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• 제14권2호
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• pp.169-172
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• 2010

Purpose: The authors have experienced various lesions that simulate ganglion of the foot on the ultrasonography. The purpose of this study is to evaluate ultrasonographic findings of soft tissue lesions, which were interpreted as ganglion but confirmed as different lesions in the foot. Materials and Methods: We reviewed a database of patients with ganglion on the ultrasonography from two different institutions. There were 109 patients who underwent both ultrasonography and surgical confirmation. Twenty one lesions were identified, of which initial interpretation on the ultrasonography included ganglion which pathology revealed to be different lesions. All images were evaluated by one musculoskeletal radiologist, regarding size, margins, internal echogenicity of lesions, and presence of posterior enhancement. Results: Of 21 lesions, there were 6 fibrous tumors including fibroma, giant cell tumor of tendon sheath, and fibromatosis, 3 hemangiomas, 2 epidermal inclusion cysts, 2 chondromas, 2 angioleiomyomas, 1 trichilemal cyst, 1 neurofibroma, 1 granular cell tumor, 1 neurilemmoma, 1 neuromyxoma, and 1 nodular hidradenoma. Mean size of the lesion was 1.1 cm. Margins were smooth in 10, mild lobulation in 8 and marked lobulation in 3 lesions. Lesions were hypoechoic in 16, anechoic in 4 and isoechoic in 1 case. Posterior acoustic enhancement was definitely present in 5 lesions. Conclusion: On the ultrasonography, various soft tissue lesions of the foot may be confused with ganglion. During surgical resection care should be given even to a simple ganglion as it might turn up to be a solid lesions such as fibrous tumors.

• Applied Microscopy
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• 제40권1호
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• pp.15-19
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• 2010

이번 연구에서는 성체 랫드(Sprague-Dawley)의 척수신경절내 분포하는 zinc의 분포 관찰하기 위하여 zinc selenium autometallograpy(AMG)로 염색하였다. H-E염색표본에서 척수신경절 속에는 신경절세포들이 무리지어 분포하고 있었고, 세포체는 둥글거나 타원형이었으며, 작은 신경절세포, 큰 신경절세포로 대별되었다. 모든 신경절세포의 세포체는 한 층의 납작한 위성세포(satellite cell)에 의하여 둘러싸여 있었다. AMG 염색표본에서는 작은 신경절세포의 경우 강한 양성반응이 핵 주변부에서 관찰되었으나, 큰 신경절세포의 경우 전반적으로 미약한 AMG 양성반응을 보였고, 염색양상도 서로 달랐다. 그러나 큰 신경절세포를 둘러싸는 위성세포에서는 뚜렷한 AMG 양성반응이 관찰되었다. 이러한 조직화학적 본 연구의 결과는 zinc가 감각신경절인 랫드 척수신경절에서 통증을 포함한 감각의 전달과정에 의미있는 기능을 영위할 수 있음을 시사한다.

• Korean Journal of Otorhinolaryngology-Head and Neck Surgery
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• 제56권12호
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• pp.749-753
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• 2013

Basically stem cells have characteristics of multi-potency, differentiation into multiple tissue types, and self-renew through proliferation. Recent advances in stem cell biology can make identifying the stem-cell like cells in various mammalian tissues. Stem cells in various tissues can restore damaged tissue. Stem cells from the adult nervous system proliferate to form clonal floating colonies called spheres in vitro, and recent studies have demonstrated sphere formation by cells in the tympanic membrane, vestibular system, spiral ganglion, and partly in the organ of Corti. The presence of stem cells in the ear raises the possibilities for the regeneration of the tympanic membrane & inner ear hair cells & neurons. But the gradual loss of stem cells postnatally in the organ of Corti may correlate with the loss of regenerative capacity and limited hearing restoration. Future strategies using endogenous stem cells in the ear can be the another treatment modality for the patients with intractable inner ear diseases.

• 대한의용생체공학회:의공학회지
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• 제36권6호
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• pp.296-301
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• 2015

Explant culture condition of dorsal root ganglion have been used to investigate the pathophysiology of peripheral nerve injury, while applying for the various clinical symptom such as trauma, pressure, and stretch. However, explant culture is usually contaminated by mitotic cells, which may observed as a newly divided cells including fibroblast or glia. The mitotic cells could be able to interrupt and change the cell signaling that make it difficult to avoid detrimental effects during the experiments. To eliminate mitotic cells, anti-mitotic reagents like mixture of uridine and 5-fluorodeoxyuridine or cytosine arabinoside were added to the cultures on the following day, but there is no research that investigate viability of anti-mitotic reagent in dorsal root ganglion explant culture. In this study, we investigate inhibition effect of cytosine arabinoside to mitotic cells in dorsal root ganglion explant culture. Also we visualized and analyzed anti-mitotic effect and toxicity of cytosine arabinoside in various concentration condition. This dorsal root ganglion explant culture condition can be applied to research that effect and mechanism of various stimulation and chemical application which affect peripheral nerve regeneration.

• Journal of Yeungnam Medical Science
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• 제20권2호
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• pp.187-196
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• 2003

The cytokines are the hormone-like proteins, which are produced in the mononuclear cells. They have many roles, such as immune mediators, cell differentiations, angiogenesis. The chemokines have chemotactic effects which control the host immune response. There were few reports about the cytokines associated with musculoskeletal tumors. From late 1980s, the cytokine studies of bone tumors such as osteosarcoma were started, but most studies for benign and malignant musculoskeletal tumors were left to be explored. To evaluate the characteristics of the cytokines in variable musculoskeletal tumors, tissues were obtained from the seven patients who visited the Yeungnam University hospital from February to July 2000. They were lipoma (1 case), parosteal osteoma (1 case), enchondroma (2 cases), pigmented villonodular synovitis (1 case), ganglion (1 case), and metastaic squamous cell carcinoma (1 case). The gene experession of the cytokines were analyzed by RNase protection assay (RPA) and reverse transcription-polymerase chain reaction (RT-PCR). The lipoma and parosteal osteoma expressed MIP-$1{\beta}$, and IP-10 genes. The two enchondromas showed different results, one expressed all of MIP-$1{\alpha}$, MIP-$1{\beta}$ and IP-10 genes but the other expressed none of above. The pigmented villonodular synovitis strongly expressed MIP-$1{\alpha}$ and IP-10 when compared with the other cases. The ganglion did not express all of the chemokines mentioned above. And the metastatic squamous cell carcinoma expressed all of the chemokines and especially IP-10 was highly expressed. Even though this study has only a few cases, these results provide a basis for the cytokine mediating network study in musculoskeletal tumors.

• 대한수의학회지
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• 제40권2호
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• pp.197-211
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• 2000

The pineal body have been known to be affected by superior cervical ganglia, and most of its nerve fibers containing peptidergic neurotransmitters have been considered to be originated from this ganglia. To confirm this relationships, some peptidergic neurotransmitters were identified in both of pineal body and superior cervical ganglia of the Korean native goat, which were divided into two group; breeding season and non-breeding season. The localizations of two catecholamine-synthesizing enzymes; tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH), were investigated by immunohistochemistry in the superior cervical ganglia and the pineal body of adult Korean native goats. Substance P (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and galanin (GAL) were also identified in these organs by immunohistochemical and double immunofluorescent methods. In superior cervical ganglia, immunoreactivities for TH and DBH were confirmed in the same ganglion cells. The immunoreactivites for SP, VIP(only in male), NPY and GAL were identified in both of ganglion cell bodies and nerve fibers in the ganglia. CGRP immunoreactivity, however, was observed only in nerve fibers. Most NPY- and VIP-immunoreactive(IR) ganglion cells also contained TH. SP and TH were colocalized in the cell bodies, but not in the nerve fibers. TH immunoreactivity was shown in almost all of ganglion cells in the superior cervical ganglia. The immunoreactivity for NPY had some seasonal variation and was stronger in breeding season than in non-breeding season. In pineal body, lots of TH-IR fibers were observed throughout the parenchyma including the pineal stalk and most of them also contained DBH. SP- and NPY-IR fibers were also immunostained with TH or DBH. But a few SP- and NPY-IR fibers were not colocalized with TH or DBH. Exceptionally, a bipolar neuron-like cell was observed to be immunostained with NPY in the pineal body. A few CGRP and GAL-IR fibers were observed, while VIP-IR fibers were not present. It is concluded that most TH- and DBH-IR fibers as well as the peptidergic immunoreactive fibers of the pineal body might be originated from the superior cervical ganglia. Some peptidergic immunoreactive fibers, however, might be come from other regions of brain. We also suggest that NPY in pineal body plays a important role for pineal function. The seasonal variation of NPY immunoreactivity indicates that the synthesis and use of NPY may be different between in breeding and non-breeding seasons.