• 제목/요약/키워드: gamma-irradiated

검색결과 1,183건 처리시간 0.025초

Utilization of Brabender Visco-Amylograph to Detect Irradiated Starches

  • Yi, Sang-Duk;Oh, Man-Jin;Yang, Jae-Seung
    • Preventive Nutrition and Food Science
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    • 제5권1호
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    • pp.20-24
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    • 2000
  • A study was carried out to establish the detection method of irradiated corn, potato, and sweet potato starches. The samples were packed in polyethylene bags and irradiated with 1, 3, 5, 7, 10, and 15 kGy using a Co-60 irradiator. The maximum viscosity of irradiated and unirradiated corn, potato, and sweet potato starches reduced by increase of irradiation dose levels and showed significant differences which clearly showed the effect of irradia-tion dose levels (p<0.05). Regression expressions and coefficients (p<0.000) or corn, potato, and sweet potato starches were y=-38.538x+718.23(r2=0.9761), y=669.97e-0.1372x (r2=0.9820) and y=-42.544x+730.26(r2=0.9939), respectively. Nor-malized parameter A,B and C values showed a dose dependent relationship and were a better parameter for detecting the irradiated starches than that of the maximum viscosity itself.

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Selection of Herbicide Tolerant Cell lines from $\gamma$-ray-Irradiated Cell Cultures in Rice (Oryza sativa L. cv. Ilpumbyeo)

  • Bae, Chang-Hyu;Lee, Young-Ill;Lim, Yong-Pyo;Seo, Yong-Won;Lee, Do-Jin;Yang, Deuk-Chun;Lee, Hyo-Yeon
    • Journal of Plant Biotechnology
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    • 제4권3호
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    • pp.123-127
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    • 2002
  • Herbicide tolerant rice (Oryza sativa L. cv. Ilpumbyeo) cell lines were selected from $\gamma$-ray-irradiated anther-derived cell cultures. The anther-derived cell clusters were small (300 to 400 ${\mu}{\textrm}{m}$ in diameter) and uniform ones that were screened by miracloth filtering. The cell suspensions were very efficient to plate one layer onto agar medium and to screen target cell lines. Herbicide tolerant cell lines were selected by 5 mg/L cyhalofop butyl (CHB) treatment by using the small cell suspensions on agar N6 medium containing 1 mg/L 2,4-D and 0.2 mg/L kinetin. Of the cell lines, one line (CHB-1) showed stable tolerance at 10 mg/L concentration after 6-month culture without herbicide suspension. Growth stability of CHB-1 was similar to that of control cell line on 10 mg/L CHB containing medium. In this experiment we established herbicide tolerant cell line selection system by using anther-derived uniform-cell suspensions with $\gamma$-ray-irradiation.

감마선 조사된 장류 물추출 분획의 유전독성학적 안전성 평가 (Genotoxicological Safety on Water-Soluble Fraction of Gamma Irradiated Korean Soybean Fermentation Foods)

  • 육홍선;이은미;김동호;이경행;변명우;이현자;이영남
    • 한국식품위생안전성학회지
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    • 제15권4호
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    • pp.297-303
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    • 2000
  • 우리나라 전통 장류인 간장, 된장, 고추장, 청국장의 위생화를 위한 방사선 조사기술의 이용 가능성을 검토할 목적으로 방사선 조사를 실시한 후 유전독성학적 안전성 실험의 일환으로 Ames test를 실시하였다. 감마선 조사 및 비조사된 간장, 된장, 고추장, 청국장 물추출 분획의 Salmonella typhimurium TA98, TA100, TA1535 및 TA1537에 대한 복귀변이 집락수를 조사한 결과, 대사활성계 도입 및 부재시 모두, 모든 시험균주에서 시험적용 농도인 10,000 $\mu\textrm{g}$/plate까지의 농도에서 복귀변이 집락수의 농도 의존적인 증가 혹은 감소를 보이지 않아 20 kGy의 고선량 감마선 조사된 간장, 된장, 고추장, 청국장은 돌연변이원성이 없는 것을 확인할 수 있었다.

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염장미역의 미생물적, 관능적 품질에 대한 감마선 조사의 영향 (Effect of Gamma Irradiation on the Microbiological and Organoleptic Qualities of Salted Sea Mustard (Undaria pinnatifida))

  • 변명우;권중호;이수정;남상명;조한옥
    • 한국식품위생안전성학회지
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    • 제6권3호
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    • pp.157-163
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    • 1991
  • 염장미역은 염농도 감소와 저장조건의 완화를 위한 연구의 일환으로서 2~4kGy의 감마선을 조사하고 ${30{\pm}2^{\circ}C와\;10{\pm}1^{\circ}C$에 저장하면서 미생물적, 관능적 품질을 평가한 결과는 다음과 같다. 염장미역의 변질에 관련된 원인 미생물은 호염성 세균과 효모로 확인되었으며, 2~4kGy의 감마선 조사는 초기의 미생물 수준을 1~2 log orders 감소시켰으나 저장 기간 중 증식에는 뚜렷한 영향이 없었다. 관능적 품질에서도 감마선 조사에 의한 미역조직의 연화현상으로 비조사군보다 낮은 기호도를 나타내어 저장 중 품질 안정성 향상에 별 효과가 없는 것으로 나타났다.

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Co$^{60}\gamma$.gamma.선이 저밀도 폴리에티렌의 각상에 미치는 조사효과 (Radiation Effect to Each Phase of Morphology on a Low Density Polyethylene Irradiated to $C_0^{60}\gamma$. ray)

  • 김봉흡;강도열;김재환
    • 전기의세계
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    • 제23권5호
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    • pp.54-60
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    • 1974
  • Proposals were mode on how to differentiate radiation effects in morphological phases of polyethylene and discussions were developed with the results obtained on a low density polyethylene, SOCAREX, specified by number average molecular weight; overbar Mn=5,400, density; 0.92, and degree of branch; 3.4/100 carbon atom, which was irradiated to Co$^{60}$ .gamma. ray at the dose rate of 0.5 Mrad/hr in ambient temperature under the pressure of 10$^{-5}$ Torr. or 1 atm. respectively. The effect to crystalline phase in possibly deduced from dose dependent variation of relative area between (110) and (200) peaks on X ray diffraction spectrum and that, the effects to amorphous phase can be understood through dose dependent relaxation behaviours of .betha. peak on internal friction characteristics of the specimen. The results obtained thus far indicate that, in crystalline phase, relative crystallinity shows a rather rapid decrease up to 20 Mrad with increasing dose, however, little change of crystallinity can be observed in the region between 20-200 Mrad, and degradation appears to be more predominant than crosslinking up to 60 Mrad. While in amorphous phase the indication also shows that degradation is only predominant up to 20 Mrad. Furthermore several correlations can be seen with amenable explanation between dose dependent behaviours observed in both phases.

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Effect of γ-Irradiation on the Physicochemical Properties of Zein Films

  • Lee, Sehee;Lee, Myoungsuk;Song, Kyung-Bin
    • Preventive Nutrition and Food Science
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    • 제8권4호
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    • pp.343-348
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    • 2003
  • To elucidate the effect of gamma-irradiation on the physicochemical properties of zein films, the molecular and mechanical properties of the films were examined after irradiation at various irradiation doses. Gamma-irradiation of zein solutions caused the disruption of the ordered structure of the zein molecules, as well as degradation, cross-linking, and aggregation of the polypeptide chains based on an SDS-PAGE study. Gamma-irradiation increased the solubility of zein and decreased the viscosity due to cleavage of the polypeptide chains. Protein solubility of the zein films in urea/2-mercaptoethanol also increased with increasing irradiation doses. Alterations of the zein molecules by irradiation decreased water vapor per-meability by 12% and increased the elongation of zein films. However, mean tensile strength of the zein films was decreased by gamma-irradiation treatment. Measurement of Hunter color values indicated that irradiation caused a destructive effect on yellow pigments, resulting in a significant decrease in Hunter b values. The microstructure as observed by scanning electron microscopy showed that irradiated zein film had a smoother and glossier surface than the non-irradiated films.

A Study on Gamma ray effects on Stress Response and Cellular Toxicity using Bacterial Cells

  • 민지호;이현주;이창우;구만복
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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    • pp.187-190
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    • 2000
  • 본 연구는 5가지의 발광성 미생물을 이용하여 유해 방사선으로 알려져 있는 ${\gamma}-rays$가 여러가지 cellular stresses 중, 특히 유전자 손상과 생물막 손상을 유발하였는데, 이들의 손상 정도가 총 방사선량과 상관관계가 있음을 발생하는 bioluminescence 로써 확인하였다. 뿐만 아니라, 선량률의 변화를 통하여 방사선으로 인한 유전자 손상 및 일반적인 독성 효과가 큰 영향을 받는 것을 확인하였는데, 선량률 증가에 따라 이들 손상정도가 증가하는 것으로 보아 선량률이 genetic 및 radioprotecion에 심각한 영향을 미치는 것을 확인하였다.

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Effects of ${\gamma}$-Irradiation on Immunological Activities of ${\beta}$-Glucan

  • Kim, Jae-Hun;Sung, Nak-Yun;Byun, Eui-Hong;Kwon, Sun-Kyu;Song, Beom-Seok;Choi, Jong-Il;Yoon, Yohan;Kim, Jin-Kyu;Byun, Myung-Woo;Lee, Ju-Woon
    • Food Science and Biotechnology
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    • 제18권5호
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    • pp.1305-1309
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    • 2009
  • This study evaluated the effects of $\gamma$-irradiation on immunomodulating properties and structural changes of ${\beta}$-glucan. ${\beta}$-Glucan solutions (10 mg/mL) were ${\gamma}$-irradiated at 10, 30, and 50 kGy. Splenocyte proliferation and cytokine (interferon-${\gamma}$ and interlukin-2) productions by ${\gamma}$-irradiated ${\beta}$-glucan were evaluated in in vivo and in vitro, and structural changes of ${\beta}$-glucan were also determined after ${\gamma}$-irradiation. ${\gamma}$-Irradiation on ${\beta}$-glucan at 50 kGy enhanced splenocyte proliferation and cytokine productions, (p<0.05) and cleft glycosidic bonds of ${\beta}$-glucan resulting in lower the molecular weight. These results indicate that the use of ${\gamma}$-irradiation on ${\beta}$-glucan may be useful for improving its immunological activity by lowering the molecular weight of ${\beta}$-glucan.

$Co^{60}-{\gamma}$ ray을 조사시킨 MOS 구조에서의 I-V특성의 방사선 조사 효과 (Radiation effects of I-V characteristics in MOS structure irradiated under $Co^{60}-{\gamma}$ ray)

  • 권순석;정수현;임기조;류부형;김봉흡
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 1992년도 추계학술대회 논문집
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    • pp.123-127
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    • 1992
  • MOS 커패시터가 이온화 방사선에 노출되었을 경우, MOS 커패시터의 방사선 조사 효과는 소자의 전기적 특성 및 동작 수명에 심각한 영향을 일으킬 수 있다. MOS 커패시터는 (100) 방향의 P-type Si wafer 위에 산화막층을 $O^2$+T.C.E. 분위기에서 성장하였으며, 그 두께는 40~80 nm로 만들었다. MOS 커패시터에 대한 방사선 조사는 $Co^{60}-{\gamma}$선을 사용하였고, 조사선량은 $10^4{\sim}10^8$으로 조사하였다. MOS 커패시터에서 전기적 전도 특성의 방사선 조사효과는 산화막 두께와 조사선량을 변화하면서 측정된 P-type MOS 커패시터는 조사선량에 의해서 강하게 영향을 받는다는 것과 저전계 영역에서의 Ohmic 전류가 전체 선량에 의존한다는 것을 알았다. 이 결과는 방사선 조사에 의해 산화막 트랩전하와 산화막-반도체($SiO_2$-Si)계면 트랩전하에 의해서 설명 할 수 있다.

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저선량 방사선에 의한 작물 초기생육 촉진효과 (Stimulation Effect of Early Growth in Crops by Low Dose Radiation)

  • 김재성;송희섭;김진규;이영근;이영복
    • 한국환경농학회지
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    • 제17권2호
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    • pp.156-159
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    • 1998
  • 작물 초기생육에 대한 저선량 방사선의 효과를 보고자 벼, 콩, 들깨 종자에 저선량 $^{\gamma}$선을 조사하여 발아율과 초기 생육을 관찰하였다. 저선량 $^{\gamma}$선에 의한 작물의 생육초기 효과는 한정되었으나 작물과 생육특성에 따라 적정 선량은 다르게 나타났다. 벼종자의 발아율은 2Gy조사구에서, 유묘초장과 생체중은 0.5Gy에서 가장 높았으며,대두종자의 발아율과 유모생육은 4Gy에서 가장 양호하였다. 들깨종자의 저선량 $^{\gamma}$선 조사에 의해 생육이 다소 불량하였으나 발아율은 2Gy에서, 유묘초장과 생체중은 1Gy에서 증가효과를 나타내었다.

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