• Korean Journal of Food Science and Technology
• /
• v.31 no.4
• /
• pp.1005-1010
• /
• 1999

To study the shortening of rigor mortis and changes in the morphological properties of gamma-irradiated pre-rigor bovine muscle (M). Sternomandibularis during post mortem, this experiment was performed with a test of shear force and the observation of the ultrastucture of raw muscle. The time elapsed until maximum shear force values was shortened by gamma irradiation, depending upon the dose. The release of rigor mortis started earlier than control (non-irradiated muscle). A shortening of the length of the Z-line and the maintenance of the sarcomere length by gamma irradiation was observed. The breakdown of the perimysium of muscle bundles was observed more in irradiated samples than in the control. In conclusion, it is considered that gamma irradiation on pre-rigor beef shortens aging-period, improves tenderness and enhances the beef quality.

• Food Science and Preservation
• /
• v.8 no.1
• /
• pp.9-15
• /
• 2001

The effects of water soaking and gamma irradiation on the storage quality of chestnuts (Castanea crenata) were investigated. Chestnuts weirs soaked in water for 48 hrs at room temperature, irradiated at 0, 0.15, 0.25 and 0.35 kGy of $\^$60/Co gamma ray, and then stored at 0$\^{C}$ and 95$\pm$2% RH for 9 months. Spouting was observed at the end of storage only in chestnuts that were soaked and irradiated at below 0.25 kGy. Rotting was found from the early stage of storage in all pre-treatment conditions, but the rotting rate of soaked chestnuts was higher twice than unsoaked ones after 9 months of storage. Weight loss was lower in soaked than unseated samples regardless of irradiation dose. Loss of flesh firmness was appreciably retarded as irradiation dose increased. Total sugar content decreased slowly in irradiated samples at the later periods of storage. Gamma irradiation resulted in the decrease of vitamin C content immediately after treatment, but retarded its loss rate during storage. Soluble tannin content was not affected by gamma irradiation at scout inhibition doses.

• Korean Journal of Microbiology
• /
• v.6 no.2
• /
• pp.54-62
• /
• 1968

The effect of gamma-ray on yeast cells Sacch. cerevisiae, and the leakage of cellular constituents such as carbohydrates, ribose, amino acids, inorganic phosphates and organic phosphates have been studied. The samples of yeast cells washed throughly and starved intensively, radiation effects were compared with those of control (un-starved), the irradiation dose rates are in the range from 24 Kr. up. to 480, Kr. The loss of 260m$\mu$. absorbing material, are also observed. Mechanisms of membrane damage by gamma-irradiation are discussed corelating to permeability changes and loss of substances, then active and passive transport process are also under considerations in discussion. The experimental results are as follows, 1. Carbohydrates of yeast cell leak out by gamma-irradiation, and amounts of loss increase proportionally as the increasing of radiation dose, curve of carbohydrates loss in starved cells is parallel with those of non-starved cells. 2. Ribose leak out less than that of carbohydrate from irradiated cell, the dose response curve of loss is straight and proportional to the increasing of radiation doses, slope of the curve is much lower than of carbohydrates. 3. Amino acids also leak out and the curve of losses to radiation is not proportional, it is revealed that there are little losses from yeast at lower doses of irradiation. 4. The losses of inorganic phosphates increase unproportionally to the increasing of irradiation doses, there are little leakage at the lower doses of irradiation. The losses of organic phosphates increase proportionally to the increasing of irradiation doses, and the amount of losses are much more than that of inorganic phosphate at lower doses of irradiation. 5. Leakage from irradiated yeast cells was shown to be due to passive transport process not an energy requiring process of ion transport. 6. Loss of 260 m$\mu$. absorbing material is little more than that of control yeast by the gamma-irradiation dose of 120K.r. and 240K.r.

• Journal of Radiation Industry
• /
• v.5 no.1
• /
• pp.55-62
• /
• 2011

We investigated the physiological responses by acute and chronic gamma-irradiation in rice. The rice, Oryza sativa L. cv Dongan, plants were irradiated with 100 and 400 Gy of gamma-rays for acute and chronic irradiation, and their morphological, chlorophyll content, MDA, proline and activities of antioxidant enzymes were examined. The plant height of chronic irradiation samples were decrease with increase of a does than the control, but the number of tiller and dry weight of shoot were increased 100 Gy. Carotenoid and chrolorphyll content were decreased of all irradiated plants than non-irradiated plants. But, MDA and proline content were increased in 400 Gy both acute and chronic gamma irradiation. And the activities of antioxidant enzymes were different as gamma-irradiation patterns.

• Journal of Radiation Industry
• /
• v.5 no.2
• /
• pp.125-130
• /
• 2011

The purpose of this study was to evaluate the protective effect of the Perilla frutescens cv. Chookyoupjaso mutant water extract (PFWE) on gamma ray-induced oxidative stress in mice. Gamma-ray is one of the sources for inducing oxidative stress. The study was divided into 6 groups with 6 mice for each treatment. Groups I and II were treated with saline (vehicle) only, groups III, IV, V, and VI were pretreated with PFWE 10, 20, 50, $50mg\;kg^{-1}$ respectively for 2 weeks before gamma radiation. And then groups II, III, IV, V were irradiated. We found that the activities of aspartate transaminase (AST) and alanine transaminase (ALT) were increased and the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were decreased by irradiation in mice. However, treatment of PFWE attenuated the activities of AST and ALT in a dose-dependent manner in irradiated mice. Furthermore, treatment of PFWE significantly increased the activities of SOD, GPx, and GR in a dose-dependent manner in irradiated mice, except for the CAT. Interestingly, the activities of GPx and GR were significantly increased by PFWE treatment. Taken together, PFWE could be effective in protecting on gamma ray-induced oxidative stress in mice.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.30 no.2
• /
• pp.239-242
• /
• 2001

Electron spin resonance(ESR) spectroscopy was used to investigate the effect of irradiation dose on the ESR signal intensity of irradiated spices and to identify the stability of radicals after storage. Red, white and black peppers, and garlic powders were irradiated with doses of 0, 1, 5, 10, 20 and 30 kGy at room temperature using a Co-60 irradiator. Triplet ESR signals were observed in irradiated pepper powders, while singlet ESR signals were observed in irradiated garlic powders. Those characteristic signals were not detected in non-irradiated samples. The strength of ESR signals linearly increased with the applied doses(1~3 kGy). Highly positive correlation coefficients ($R^2$=0.9757~0.9933) were obtained between the irradiation doses and the corresponding ESR signal intensities. The signal intensities of irradiated samples were stable even after 97 days of storage at room temperature.

• Korean Journal of Food Science and Technology
• /
• v.32 no.4
• /
• pp.822-827
• /
• 2000

Gamma irradiation was applied to reduce shrimp allergy. Shrimp heat-stable protein(HSP) and shrimp protein extract were gamma-irradiated at 1, 3, 5, 7 or 10 kGy in an aqueous state (1.0 mg/mL). The changes in allergenic and antigenic properties of protein extract and HSP resulted from gamma irradiation were monitored by ELISA with mouse mAb or human patients sera and immunoblotting. Conformational changes in irradiated HSP were measured by both GPC-HPLC and SDS-PAGE. The binding ability of shrimp allergic patients IgE to irradiated protein extract or irradiated heat-stable protein was dose-dependently reduced. When measured by gel permeation chromatography and sandwich ELISA, the amount of intact heat-stable protein in the irradiated solution was reduced by gamma irradiation depending upon the applied dose. SDS-PAGE showed that the main band disappeared and new bands appeared in a higher molecular weight zone. The results provide a new possibility to use irradiation process for reducing the allergenicity of shrimp.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.31 no.5
• /
• pp.723-727
• /
• 2002

Conformational changes of allivin, a major allergen protein in garlic, by gamma irradiation were observed. Allium solution (3.0 mg/mL in 0.01 M PBS, pH 7.4) and raw garlic were irradiated in a gamma irradiation facility to be absorbed with doses of 2.5, 5.0, 7.5 and 10.0 kGy. After irradiation, a half of irradiated allivin solution was filtered through 0.22 ${\mu}{\textrm}{m}$ filtration kit. UV spectrum and SDS-PAGE were used for observing the conformational changes of allivin by gamma irradiation. Turbidity of allivin solution was increased by optical observation, and the result was validated by the increase of absorbance at 660 m. Turbidity of irradiated and filtered allivin solution significantly (p<0.05) decreased, and the concentration of soluble protein in the solution decreased by increase of the dose. Aggregation among allivin molecules was observed by SDS-PAGE, but irradiated and filtered samples showed decrease of allivin with reducing aggregation trend. SDS-PAGE profiles of irradiated raw garlic were not different. Results indicate that gamma irradiation can decrease the allivin in solution state which may impact on allergenic reaction of garlic.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.35 no.1
• /
• pp.104-108
• /
• 2006

Effect of gamma irradiation on the softening of dried fernbrake at different moist-heating conditions was investigated. Dried fernbrake packaged in controlled atmosphere $(CO_2\;25\%,\;N_2\;75\%)$ was irradiated up to 7 kGy with $\gamma-ray$. Hardness of dried fernbrake was significantly decreased at the irradiation dose of above 5 kGy. Hardness of cooked fernbrake was significantly decreased with increasing irradiation dose level as well as heating temperature and time. $DT_{50}$ value (The heating time required to reach the $50\%$ reduction of hardness) for irradiated fernbrake was shortened according to dose level as well as heating temperature and heating time: $DT_{50}$ value of control: 23 min, 5 or 7 kGy: $2.2\~5.0$ min at $60^{\circ}C$. Also, the activation energy for softening of irradiated fernbrake ($1.85\~1.88$ kcal/mole for $5\~7kGy$) decreased compared to control (4.30 kcal/mole). Moisture content, swelling and rehydration rate of gamma irradiated fernbrake during moist heating increased according to the irradiation doses. Sensory results showed that scores of off-flavor and odor in irradiated fernbrake upto 7 kGy were not significantly different from control. Based on these results, gamma irradiation was effective for the reduction of cooking time and the activation energy for softening of dried fernbrake with increasing dose levels.

• Korean Journal of Food Science and Technology
• /
• v.32 no.5
• /
• pp.1128-1134
• /
• 2000

This study was carried out to evaluate quality properties of gamma-irradiated Kwamegi prepared from Cololabis seira semi-dried by cold sea wind. Edible portion of Kwamegi was prepared from whole body, vacuum-packaged, gamma-irradiated in the doses of 3 or 5 kGy, and stored at $5^{\circ}C$ for 60 days. Volatile basic nitrogen and trimethylamine contents were not different by gamma irradiation. During storage increase of those two compounds were inhibited depending upon the dose. Thiobarbituric acid values did not differ in all samples, regardless of irradiation and storage. The amount of total volatile compounds of Kwamegi decreased by irradiation. Rheological properties were not affected by irradiation and were maintained up to 60 day when the Kwamegi was irradiated at 5 kGy dose, but those of control was softened. Sensory evaluation had no differences in all samples immediately after irradiation. Sensory quality of Kwamegi irradiated were organoleptically adequate, however that of control was deteriorated. In conclusion, these results indicate that gamma irradiation technique can be used to maintain the quality of Kwamegi.