• Title/Summary/Keyword: gamma-irradiated

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The Effect of Gamma-Irradiation on Aqueous Solutions of Triglycine 2. Electrophoretic and Paper Chromatographic Study of Irradiated Triglycine (Triglycine 수용액에 미치는 감마선의 영향 2. Electrophoresis 와 Paper Chromatogrphay 에 의한 연구)

  • LEONE, Charles A
    • The Korean Journal of Zoology
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    • v.7 no.1
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    • pp.1-5
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    • 1964
  • GamnH-irradiated aqueous solutions of triglycine were studied using low voltage electrophoresis, paper chromatography, and determination of carbonyl compound. Four generalizations can be made as follows: 1. One component from irradiated triglycine formed a complex with copper ions and had higher electronegativity than did triglycine. 2. Five components from irradiated triglycine were separated by paper chromatography; of these, two were identified. 3. Carbonyl compound formation in irradiated triglycine increased up to $5{\times}10^{20}\;ev/ml$ and decreased at higher doses up to $1.9{\times}10^{21}\;ev/ml$ indicating its participation in secondary reaction in the complex system. 4. The possibility of hydrolytic brcabtgc of peptide bond was indicated by the separation of glycine from irradiated triglycine.lycine.

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Investigation of Different Factors Affecting the Electron Spin Resomance-based Characterization of Gamma-irradiated Fresh, White, and Red Ginseng

  • Ahn, Jae-Jun;Akram, Kashif;Jo, Deok-Jo;Kwon, Joong-Ho
    • Journal of Ginseng Research
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    • v.36 no.3
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    • pp.308-313
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    • 2012
  • Fresh (raw roots), white (dried), and red (steamed-drid) ginseng samples were gamma-irradiated at 0 to 7 kGy. Electron spin resonance (ESR) technique was used to characterize the irradiation status of the samples, targeting the radiation-induced cellulose radicals after different sample pretreatments. All non-irradiated samples exhibited a single central signal (g=2.006), whose intensity showed significant increase upon irradiation. The ESR spectra from the radiation-induced cellulose radicals, with two side peaks (g=2.0201 and g=1.9851) equally spaced (${\pm}3mT$) from the central signal, were also observed in the irradiated samples. The core sample analyzed after alcoholic-extraction produced the best results for irradiated fresh ginseng samples. In the case of irradiated white and red ginseng samples, the central (natural) and radiation-induced (two-side peaks corresponding to cellulose radical) signal intensities showed little improvement on alcoholic-extraction. The water-washing step minimized the effect of $Mn^{2+}$, but reduced the intensity of side peaks making them difficult to indentify. The effect of different origins was negligible, however harvesting year showed a clear effect on radiation-induced ESR signals.

Detection of Gamma-Irradiation Treatment in Imported Spices by ESR Spectroscopy (ESR spectroscopy에 의한 감마선 조사된 수입 향신료의 검지)

  • Han, Jeong-Eun;Lee, Eun-Jeong;Yang, Jae-Seung
    • Korean Journal of Food Science and Technology
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    • v.35 no.6
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    • pp.1060-1063
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    • 2003
  • Electron spin resonance (ESR) spectroscopy was used to investigate the effects of irradiation doses and storage times on three spices. Three imported spices (Brassica juncea, Origanum vulgare, Laurus nobilis) were irradiated with doss of 0, 1, 5 and 10 kGy using a Co-60 irradiator at room temperature. ESR signals were measured from each sample by a Bruker-EPR spectrometer. The gamma-irradiated Brassica and Laurus nobilis exhibited characteristic signals, which were different from those of the non-irradiated ones. Non-irradiated and irradiated Origanum vulgare exhibited single lines, with higher ESR signals in irradiated sample than in non-irradiated sample. The ESR signals increased linearly with increasing irradiation doses $(1{\sim}10kGy)$. A strong positive correlation coefficient $(R^2=0.9939{\sim}0.9993)$ was obtained between the irradiation doses and the corresponding ESR signal intensities. Although the ESR signal intensities decreased with storage time, signals from the three irradiated spices were observed even after 12 weeks of storage at room temperature.

Quality Evaluation of Sliced and Pizza Cheeses Treated by Gamma and Electron Beam Irradiation

  • Kim, Hyun-Joo;Ham, Jun-Sang;Kim, Kee-Hyuk;Ha, Ji-Hyoung;Ha, Sang-Do;Jo, Cheo-Run
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.8
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    • pp.1112-1117
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    • 2010
  • This study was conducted to evaluate and compare the quality changes of commercial sliced and pizza cheeses processed by gamma and electron beam irradiation. The $L^*$-value of sliced and pizza cheeses decreased and the $a^*$-value decreased only in pizza cheese by both irradiation sources. There was no change in pH. There was no difference in 2-thiobarbituric acid reactive substances (TBARS) value between non-irradiated and irradiated samples at a dose of 3 kGy or less (p<0.05). However, both irradiation sources resulted in increased TBARS value in sliced and pizza cheeses at 5 kGy. Sensory evaluation revealed that irradiation influenced odor, taste and overall acceptability of both cheeses and may cause the limitation of consumers' acceptance for irradiated cheese products. Results indicate that both gamma and electron beam irradiations with less than 3 kGy may not influence significantly the physicochemical quality of sliced and pizza cheeses. However, to meet a market requirement, a method to overcome the sensory deterioration of cheeses should be developed and applied.

Assurance on the Genotoxicological Safety of Fermented Vegetables Pasteurized by Gamma Irradiation

  • Yook, Hong-Sun;Byun, Myung-Woo;Song, Hyun-Pa;Lee, Ju-Woon;Kim, Kwan-Soo;Kim, Kwang-Hoon;Lee, Ho-Joon;Kim, Dong-Ho
    • Food Science and Biotechnology
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    • v.14 no.1
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    • pp.137-142
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    • 2005
  • The genotoxicological safety of fermented vegetables pasteurized by gamma irradiation was examined to consider the possibility of the application of irradiation for extending of fermented vegetables. A fermented vegetable was irradiated at 20 kGy to assure its toxicological safety even at a high dose of radiation. The Ames test with Salmonella typhimurium (TA98, TA100, TA1535, TA1537) and Escherchia coli (WP2), and the chromosomal aberration test in Chinese hamster lung (CHL) cells were performed. In vivo micronucleus test were conducted in mouse bone marrow cells. With or without metabolic activation, negative results were obtained in the Ames test and the chromosomal aberration test. In the micronucleus test, there was no enhancement in the formation of micronucleus, and there were no such significant differences between the irradiated and non-irradiated samples. The observed results indicated that, a level of 20 kGy of gamma irradiation on the fermented vegetable did not bring about any genotoxic effects under the described experimental conditions.

Hyperoside Protects Cells against Gamma Ray Radiation-Induced Apoptosis in Hamster Lung Fibroblast

  • Piao, Mei Jing;Kim, Ki Cheon;Cho, Suk Ju;Chae, Sungwook;Kang, Sam Sik;Hyun, Jin Won
    • Natural Product Sciences
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    • v.19 no.2
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    • pp.127-136
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    • 2013
  • Ionizing radiation, including that evoked by gamma (${\gamma}$)-rays, induces oxidative stress through the generation of reactive oxygen species, resulting in apoptosis, or programmed cell death. This study aimed to elucidate the radioprotective effects of hyperoside (quercetin-3-O-galactoside) against ${\gamma}$-ray radiation-induced apoptosis in Chinese hamster lung fibroblasts, V79-4 and demonstrated that the compound reduced levels of intracellular reactive oxygen species in ${\gamma}$-ray-irradiated cells. Hyperoside also protected irradiated cells against DNA damage (evidenced by pronounced DNA tails and elevated phospho-histone H2AX and 8-oxoguanine content) and membrane lipid peroxidation. Furthermore, hyperoside prevented the ${\gamma}$-ray-provoked reduction in cell viability via the inhibition of apoptosis through the increased levels of Bcl-2, the decreased levels of Bax and cytosolic cytochrome c, and the decrease of the active caspase 9 and caspase 3 expression. Taken together, these results suggest that hyperoside defend cells against ${\gamma}$-ray radiation-induced apoptosis by inhibiting oxidative stress.

Rheological Properties of Gamma Irradiated Arrowroot (Pueraria thunbergina. B) Starch (감마선 조사 칡 전분 gel의 rheology 특성)

  • Kuhm, Herena;Lim, Jin-Hyuk;Lee, Eun-Ju;Chang, Kyu-Seob
    • Korean Journal of Food Science and Technology
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    • v.36 no.5
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    • pp.740-743
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    • 2004
  • Rheological properties of gamma-irradiated arrowroot starch was examined to utilize as fundamental research data far processing. Irradiated arrowroot starch solutions (3, 4, 5, 6%) were gelatinized at $95^{\circ}C$ for 40 min, and its flow properties measured using rheometer at $30^{\circ}C$ and 10 to 200 rpm rotation rate. Rheological parameters of irradiated arrowroot starch gelatinized solution were calculated using Herschel-Bulkley equation. Gelatinized arrowroot starch solutions irradiated at $0^{\circ}C$ and 5kGy showed pseudoplastic fluid behavior, while those irradiated at 10, 20, and 30kGy were the dilatant with ${\tau}_y=0$ (yield stress).

Properties of Pulsed Photostimulated Luminescence and Thermoluminescence for Detection of Gamma-Irradiated Teas during Storage

  • Kausar, Tusneem;Kim, Byeong-Keun;Yang, Jae-Seung;Byun, Myung-Woo;Kwon, Joong-Ho
    • Preventive Nutrition and Food Science
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    • v.9 no.3
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    • pp.227-231
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    • 2004
  • Green, black and oolong teas were irradiated by $^{60}$ Co-gamma rays (0~10 kGy) and were investigated for detection of irradiation treatment using pulsed photostimulated luminescence (PPSL) and thermoluminescence (TL) during storage. Teas irradiated at 2.5 kGy or more showed a photon count of greater than 5000 counts/60 sec while the non-irradiated yielded only 650~1000 count/60 sec. Correlation coefficients between irradiation dose and photon counts/60 sec were 0.8951, 0.7934 and 0.9007 for green, black and oolong teas, respectively. The TL glow curves for minerals isolated from the non-irradiated teas were situated at about 30$0^{\circ}C$ with a low intensity, but for irradiated samples were approximately 15$0^{\circ}C$ with a high intensity. The TL ratios (TL$_1$/TL$_2$), calculated from values after initial radiation and then after re-irradiation of the teas, were below 0.1 for the non-irradiated samples and higher than 1.44 for all irradiated samples, enhanced the reliability of the identification results for TL. The signal intensity of PPSL and TL for irradiated teas decreased with the lapse of post-irradiation storage time at room temperature but was still distinguishable from that of the non-irradiated samples even after one year.

Radiation effect on the corrosion of disposal canister materials

  • Minsoo Lee;Junhyuk Jang;Jin Seop Kim
    • Nuclear Engineering and Technology
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    • v.56 no.3
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    • pp.941-948
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    • 2024
  • The effects of radiation on the corrosion of canister materials were investigated for the reliable disposal of high-level radioactive waste. The test specimens were gamma-irradiated at a very low dose rate of approximately 0.1 Gy/h for six and twelve months. The copper and cast iron species were less corroded when irradiated. It is hypothesized that gamma rays suppress the formation of lower-enthalpy species like metal oxides and activate reductive reactions. In contrast, it was difficult to evaluate the effect of radiation on the corrosion of titanium and stainless steel.

Reduction of Immune Toxicity of LPS by Gamma Irradiation (감마선 조사에 의한 LPS의 면역 독성 저감화)

  • Park, Jong-Heum;Sung, Nak-Yun;Byun, Eui-Beak;Song, Du-Sup;Song, Beom-Seok;Kim, Jaekyung;Lee, Ju-Woon;Park, Sang-Hyun;Kim, Jae-Hun;Kang, Geon-Ok;Yoo, Young-Choon
    • Journal of Radiation Industry
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    • v.6 no.3
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    • pp.217-221
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    • 2012
  • The purpose of this study is to reduce the immune toxicity of lipopolysaccharide (LPS) by gamma irradiation. LPS was gamma-irradiated at the various doses of 20, 100 and 200 kGy and then evaluated on the immune toxicity through the cell proliferation, nitricoxide production and cytokine release. Cell proliferation significantly decreased in the intact LPS treated groups, whereas gamma-irradiated LPS treated group were not reduced the cell proliferation. Similarly, the production of nitric oxide and cytokine showed the high levels in the intact LPS treated group. However, gamma-irradiated LPS treated group remarkably decreased the production of nitric oxide and cytokine in dose-dependent manner. Therefore, gamma irradiation may effective method to reduce the immune toxicity of LPS.