• 한국염색가공학회지
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• 제25권4호
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• pp.271-278
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• 2013

In order to investigate the effect of gallotannin treatment to ceramide-containing fabrics on atopic skin diseases, the agglomeration of standard protein BSA and the deactivation of model enzyme were examined. The gallotannin treated on ceramide-containing fabrics precipitated the standard protein, BSA, and therefore deactivated the model enzyme by 70% at 6% treatment concentration. Wash durability should be improved after around 5 cycles of washing. Clinical test of the gallotannin-treated fabrics was carried out on mice for two test items, transepidermal water loss assay and severity score of diseased skin of mice. The results showed significant level of improvement of atopic skin diseases compared with the negative controled.

• Journal of the Korean Wood Science and Technology
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• 제43권5호
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• pp.558-565
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• 2015

Katsura tree (Cercidiphyllum japonicum Sieb. Et Zucc) leaves were collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, methylene chloride ($CH_2Cl_2$), ethylacetate (EtOAc), and $H_2O$. The EtOAc fraction was chromatographed on a Sephadex LH-20 column with various aqueous MeOH eluting solvents and finally treated with acetone-$H_2O$ (7:3, v/v) to isolate a gallotannin. According to the NMR analysis, including HSQC and HMBC, and with the comparison of authentic literature data, the isolate was elucidated as 6-m-digalloyl-1,2,3,4-tetra-O-galloyl ${\beta}$-D-(+)-glucose, one of hydrolyzable tannins and one of gallotannins. The compound was only gallotannin which was firstly isolated from the extracts of Katsura tree leaves, and has not been reported before in domestic tree sources.

• 한국염색가공학회지
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• 제13권1호
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• pp.1-8
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• 2001

Amur cork tree is natural basic and yellow dye largely used for dyeing on cotton. The most effective solvent for extract of berberine from amur cork tree was methanol. The dyeabilities of the colorant on cotton and the fastness of cotton fabrics dyed with amur cork tree extract were investigated. And according to the concentration, temperature and time of pre-treatment, K/S values, wash fastness and Hand Value of cotton fabrics dyed with amur cork tree extract were investigated. As a results, K/S values of dyed cotton fabrics were increased by Pre-treatment with chinese gallotannin and the optimum condition of chinese gallotannin treatment was 15%owf concentration at $60^\circ{C}$, 30min. The wash fastness was enhanced to 3.5 grades. There was no degradations of Hand Value of cotton fabrics treated with chinese gallotannin.

• 한국염색가공학회지
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• 제13권5호
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• pp.32-32
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• 2001

Amur cork tree contains yellow natural basic dye largely used for dyeing on silk. Dyeabilities and fastnesses of the colorant on silk fabrics were investigated , According to dye concentration, pH, temperature, time of pre- and post- treatment of mordant, K/S values and fastnesses of the silk fabrics were examined. As a results, K/S values of dyed silk fabrics were increased by pre- and post-treatment with chinese gallotannin. The optimum condition of chinese gallotannin pre-treatment was 0.15% concentration, pH 4, 70℃, and 45min, and that of post-treatment was 0.05% concentration,60℃, and 30min. The wash fastness and the light fastness were enhanced to 4 grades and 2∼3 grades.

• 한국염색가공학회지
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• 제13권5호
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• pp.320-328
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• 2001

Amur cork tree contains yellow natural basic dye largely used for dyeing on silk. Dyeabilities and fastnesses of the colorant on silk fabrics were investigated , According to dye concentration, pH, temperature, time of pre- and post- treatment of mordant, K/S values and fastnesses of the silk fabrics were examined. As a results, K/S values of dyed silk fabrics were increased by pre- and post-treatment with chinese gallotannin. The optimum rendition of chinese gallotannin pre-treatment was 0.15% concentration, pH 4, $70^\circ{C}$, and 45min, and that of post-treatment was 0.05% concentration,$60^\circ{C}$, and 30min. The wash fastness and the light fastness were enhanced to 4 grades and 2~3 grades.

• Animal cells and systems
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• 제16권5호
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• pp.366-375
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• 2012

Gallotannin (GT) is derived from plant poly phenol and is associated with biological actions in a wide range of cells. In this study, we evaluated the effect of GTon apoptosis and cyclooxygenase-2 (COX-2) expression and attempted to shed light on the mechanism of action in A549 human lung carcinoma cells. We found that GT dramatically induced apoptosis as demonstrated by expression of p53 and active caspase-3 via western blot analysis and fragmented DNA as detected by DNA fragmentation and DAPI staining. We also observed that GT significantly causes COX-2 expression in a dose-dependent manner determined by western blot analysis. Phosphorylation of Akt and p38 was considerably increased by GT in A549 human lung carcinoma cells. Inhibition of Akt and p38kinase with LY294002 or SB203580 suppressed GT-induced apoptosis and COX-2 expression. Furthermore, we have shown that prevention of COX-2 with NS398 or indomethacin does not any effects on apoptosis induced by GT. Taken together, our present results suggest that GT regulates apoptosis and COX-2 expression through Akt and p38kinase pathway in A549, human lung carcinoma cells.

• 한국식품영양과학회지
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• 제45권4호
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• pp.613-618
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• 2016

1,2,3,4,6-Penta-O-galloyl-${\beta}$-D-glucose(PGG)는 오배자(Galla Rhois)의 gallotannin으로 항산화 효과, 항균 효과, 항알레르기 효과 등을 가지는 것으로 알려졌다. 알레르기성질환은 비만세포를 포함한 다양한 면역세포와 관련된 질환이다. 비만세포는 탈과립화에 의한 알레르기 매개성 물질(histamine 및 ${\beta}-hexosaminidase$)의 분비, T helper 2 type 사이토카인(IL-4) 분비 및 염증을 일으키는 매개체($TNF-{\alpha}$)의 증가를 통해 알레르기 반응에서 중요한 역할을 한다. 따라서 본 연구에서는 PGG가 phorbol 12-myristate 13-acetate(PMA)와 A23187로 비만세포인 RBL-2H3 세포에서 탈과립 그리고 Th2 type 및 염증성 사이토카인 생산에 미치는 영향을 조사하고, 관련 메커니즘에 대해 평가하였다. PGG는 PMA와 A23187 자극에 의한 탈과립 마커(histamine 및 ${\beta}-hexosaminidase$)의 분비를 억제하고, IL-4 및 $TNF-{\alpha}$와 같은 사이토카인의 분비를 억제하였다. 이러한 효과는 전사인자인 $NF-{\kappa}B$의 세포질에서 핵으로의 이동을 억제함으로써 나타나는 것으로 판단된다. 이러한 결과로부터 gallotannin의 하나인 PGG가 알레르기 반응을 현저히 저해하는 효과가 있는 것으로 나타나, 향후 알레르기성 질환을 예방, 개선 및 치료하는 데 유용한 물질로 사용될 가능성이 있을 것으로 생각된다.

• 한국염색가공학회지
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• 제13권5호
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• pp.1-1
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• 2001

As the basic study to investigate the mechanism of improvement in the dyeing fastness of natural dyes on silk fabrics the optical behavior of tannin solution, the optimal condition of tannin treatment and the changes of the physical structure of silk fabrics were studied in the treatment of tannin. Results obtained were as follows ; 1.The K/S values of tannin-treated silk fabrics were not changed by tannin content on silk fabrics but yellowing of tannin in treatment condition. 2. As the concentration of tannin was increased in its treating condition, the tannin content and K/S vague of tannin-treated silk fabrics were increased. 3. Because tannin gave rise to yellowing by hydrolysis and oxidation at high temperature, the higher tannin-treating temperature was, the lower the tannin content and K/S values of silk fabrics 4. The tannin content of silk fabric was higher in acidic treating condition and the highest K/S value was showed at pH 5. 5. As the tannin content of silk fabric was increased, the value of ζ -potential of it became negative. 6. As the tannin content of silk fabric was increased, the crystallinity and crystallite size of it were decreased. This meant that the fraction of amorphous region in the fibers increased. And the degree of orientation of it was decreased with the tannin treatment.

• 한국염색가공학회지
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• 제13권5호
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• pp.289-297
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• 2001

As the basic study to investigate the mechanism of improvement in the dyeing fastness of natural dyes on silk fabrics the optical behavior of tannin solution, the optimal condition of tannin treatment and the changes of the physical structure of silk fabrics were studied in the treatment of tannin. Results obtained were as follows ; 1 The K/S values of tannin-treated silk fabrics were not changed by tannin content on silk fabrics but yellowing of tannin in treatment condition. 2. As the concentration of tannin was increased in its treating condition, the tannin content and K/S vague of tannin-treated silk fabrics were increased. 3. Because tannin gave rise to yellowing by hydrolysis and oxidation at high temperature, the higher tannin-treating temperature was, the lower the tannin content and K/S values of silk fabrics 4. The tannin content of silk fabric was higher in acidic treating condition and the highest K/S value was showed at pH 5. 5. As the tannin content of silk fabric was increased, the value of $\xi$ -potential of it became negative. 6. As the tannin content of silk fabric was increased, the crystallinity and crystallite size of it were decreased. This meant that the fraction of amorphous region in the fibers increased. And the degree of orientation of it was decreased with the tannin treatment.

• Natural Product Sciences
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• 제10권6호
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• pp.330-334
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• 2004

Four isoflavonoids and three flavonoids, and a gallotannin were isolated from the fruits of Sophora japonica (Leguminosae). Their structures were identified as genistein (1), sophoricoside (2), genistein-4'-O--L-rhamnopyranoside (3), $genistein-4'-O-{\alpha}-L-rhamnopyranosly-(1{\rightarrow}2)-{\beta}-D-glucopyranoside$ (4), $kaempferol-3-O-{\alpha}-D-sophoroside$ (5), $kaempferol-3-O-{\beta}-D-glucopyranosyl-(1{\rightarrow}2)-{\alpha}-L-rhamnopyranosly-(1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (6), rutin (7) and gallic acid $4-O-{\beta}-D-(6'-O-galloyl)-glucopyranoside$ (8) by chemical and spectroscopic analysis and comparisons with previously reported spectral data. Compounds 3 and 8 were isolated for the first time from this plant. Anti-oxidative activity was evaluated for the isolated compounds. 8 exhibited potent anti-oxidative activity against the radical scavenging ability of DPPH with the $IC_{50}$ value of $17.1\;{\mu}g/ml$.