• Biotechnology and Bioprocess Engineering:BBE
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• 제5권3호
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• pp.215-218
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• 2000

Sialytrisaccharides based on $\beta$-galactosyldisaccharides were synthesized using $\beta$-galactosidase and trans-sialidase in one pot. Using $\beta$-galactosidase from Bacillus Ciculans and trans-sialidase from Trypanosoma cruzi simulaneously, 6mM sialyltrisaccharides composed of about 95% NeuAc$\alpha$(2,3)Gal$\beta$(1,4)GlcNAc and 5% NeuAc$\alpha$(2,3)Gal$\beta$(1,6)GlcNAc were produced from a reaction mixture containing 25mM o-nitropheny1-$\beta$-D-galsctolneuraminic acid. One beauty of this reaction was that a secondary hydrolysis of the disaccharide intermediate occurring between the activated galactopyranoside and N-acetylgucosamine was prevented. Using $\beta$-galactosidase from Escherichia cloi and the same trans-sialidase, 15mM sialyltrisaccharides composed of about 90% NeuAc$\alpha$(2,3)Gal$\beta$(1,6)GlcNac and 10% NeuAc$\alpha$(2,3)Gal$\beta$(1,4)GlcNAc were produced from a reaction misture containing 400nM galactose, 800nM N-acetylglucosylation rection between galactose and N-actylgucosamine was diminant since the disaccharide intermediate mainly resulted sreulted in the silylated product.

• 대한공간정보학회지
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• 제18권1호
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• pp.89-97
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• 2010

삼면이 바다로 둘러싸인 우리나라에서 정밀 지오이드 모델 개발을 위해서 해상에서의 중력자료는 필수적인 요소이다. 기존의 연구에서 해상에서의 중력자료는 위성고도계자료에서 산출한 중력자료를 이용하여 처리하였으나, 측량장비의 발달과 측위 시스템의 개선 및 국내 선상중력자료의 취득으로 인해 보다 정밀한 중력 자료의 취득이 가능하게 됨에 따라 선상중력자료의 정밀도를 검증하고, 지오이드의 구축에 있어서 선상중력자료의 활용성을 분석해볼 필요가 있다. 이를 위해 국립해양조사원에서 지난 1997년부터 2007년까지 측정한 선상중력자료를 대상으로 에트뵈스(Eotvos) 효과 보정, 칼만 필터(Kalman Filter), 교차점 보정 등의 일관된 처리를 거쳐 평균 16.47 mGal, 표준편차 18.86 mGal의 프리에어이상값을 산출하였다. 산출된 선상중력 프리에어이상값의 정밀도는 DNSC08 위성고도계자료와 비교를 통하여 검증되었는데, 두 자료의 프리에어이상값의 차이는 평균 -0.88 mGal, 표준편차가 9.46mGal로 나타났다. 이러한 차이는 영외지역이나 섬의 분포가 많은 해안 지역에서의 선상중력자료를 확보하는데 있어서의 한계와 해저지형에 의한 영향이 상대적으로 선상중력자료에서 크게 나타나기 때문으로 판단된다. 향후 지오이드 모델 개발에 선상중력자료를 활용하기 위해서는 자료 취득의 한계를 극복하고 위성고도계 자료와의 연계에 대한 연구가 진행되어야 할 것이다.

• 한국수정란이식학회지
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• 제27권1호
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• pp.9-14
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• 2012

This study was conducted to analyze the transgenic efficiency and sex ratio in ${\alpha}$-1,3-galactosyltransferase (GalT) knock-out (KO) transgenic pigs according to generation. GalT KO piglets were produced by artificial insemination or natural mating. The transgenic confirmation of GalT KO was evaluated by PCR amplification using specific primers. After electrophoresis, three types of bands were detected such as 2.3 kb single band (Wild), 2.3 and 3.6kb double bands (GalT KO -/+; heterozygote), and 3.6kb single band (GalT KO -/-; homozygote). Transgenic efficiency in F1 generation was 64.5% (23/35) of GalT KO (-/+). In F2 generation, GalT KO transgenic efficiency was 36.4% (21/57, Wild), 47.5% (28/57, GalT KO -/+), and 16.1% (8/57, GalT KO -/-), respectively. Interestingly, no homozygote piglets were born in 6 deliveries among total 11 deliveries, although they were pregnant between male (M) and female (F) $F_1$ heterozygote. In the 5 litters including at least one GalT KO -/- piglet, the transgenic efficiency was 13.3% (2/24, Wild), 51.3% (14/24, GalT KO -/+), and 35.3% (8/24, GalT KO -/-), respectively. The sex ratio of M and F was 40:60 in $F_1$ and 49:51 in $F_2$ generation, respectively. Based on these results, GalT KO transgenic pigs have had a reproductive ability with a normal range of transgenic efficiency and sex ratio.

• 한국의류학회지
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• 제23권1호
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• pp.30-41
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• 1999

This study was conducted to verify the advantages of Gal-Ot. Gal-Ot is defined the clothings dyed with persimmon juice. Firstly the physical properties of fabrics were examined. Secondly the wear tests were conducted. The wear tests were performed in climatic chamber controlled 3$0^{\circ}C$ air temperature 50$\pm$5% R.H and 0.2m/s air movement. Four women subjects participated in this experiment and 4 times experiments were performed per one subject. The expeimental schedule was planned following 4 steps that is sedentary posture during 30 minutes-walking the 5。slope treadmill by 70m/min during 20 minutes-sedentary posture during 20 minutes-standing posture on toward the blowing wind during 10 minutes. The results obtained is as folows : The insulation of fabric was increased with dyeing with persimmon juice. The air permeability of fabric was remarkably increased with dyeing with persimmon juice. The mean skin temperatures of subjects were apt to be higher in wearing Gal-Ot than undyed clothing. The clothing micro temperature of subjects were slightly inclined to become lower in wearing Gal-Ot than undyed clothing, The clothing micro humidity was decreasee when: the subjects were gal-Ot than wore undyed clothing, And then it brought about more comfortable on wear sensation. The differences of physiological reactions were not consistent between clothing types except for sweat rate on the back. This result may be attributed the to complex experimental schdules consisted of four steps and to a little difference between open type and close type. However I consider that the clothing type of Gal-Ot is suitable for open type because Gal-Ot is summer wear for blocking the sultry heat.

• Fisheries and Aquatic Sciences
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• 제25권11호
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• pp.559-571
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• 2022

Galectins, a family of ß-galactoside-binding lectins, have emerged as soluble mediators in infected cells and pattern recognition receptors (PRRs) responsible for evoking and regulating innate immunity. The present study aimed to evaluate the role of galectin-1 in the host immune response of redlip mullet (Liza haematocheilia). We established a cDNA database for redlip mullet, and the cDNA sequence of galectin-1 (LhGal-1) was characterized. In silico analysis was performed, and the spatial and temporal expression patterns in gills and blood in response to lipopolysaccharide polyinosinic:polycytidylic acid, and Lactococcus garvieae were estimated via quantitative real-time PCR. Functional assays were conducted using recombinant protein to investigate carbohydrate binding, bacterial binding, and bacterial agglutination activity. LhGal-1 was composed of 135 amino acids. Conserved motifs (H-NPR, -N- and -W-E-R) within the carbohydrate recognition domain were found in LhGal-1. The tissue distribution revealed that the healthy stomach expressed high levels of LhGal-1. The temporal monitoring of LhGal-1 mRNA expression in the gill and blood showed its significant upregulation in response to immune challenges with different stimulants. rLhGal-1 exhibited binding activity in response to carbohydrates and bacteria. Moreover, the agglutination of rLhGal-1 against Escherichia coli was observed. Collectively, our findings suggest that LhGal-1 may function as a PRR in redlip mullet. Furthermore, LhGal-1 can be considered a significant gene to play a protective role in redlip mullet immune system.

• 혜화의학회지
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• 제16권1호
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• pp.107-114
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• 2007

1. So-gal IS also called as so-jung, So-dan, Pe-so, Jung-so. Gyuk-so. 2. Cause of So-gal is impairment of Jin-Aek. induced by mal intake. stress, overstrain, intoxication, aging. The process is done by Cho-yeol. 3. Symptoms of So-gal is classified m to three categories Sang-So stands for polydipsia, Jung-So stands for polyphagia and weight loss, and Ha-So stands for polyuria. 4. In treating of So-gal, Chung-Hwa Bo-eum Bo-yang is the main principle. The fundamental basis of treating So-gal is Chi-shin. 5. It is not easy to perfectly classify symptoms of So-gal into three categories Therefore further inquiry is required on classification methods of So-gal.

• 운동과학
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• 제26권3호
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• pp.223-228
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• 2017

PURPOSE: This study investigated the protective role of high-intensity interval training against acute liver injury induced by D-galactosamine (D-Gal)/lipopolysaccharide (LPS). METHODS: A total of 30 male BALB/c mice aged 5-week were randomly assigned to high-intensity, interval training group (EX, n=10) or control group in cage (Non-EX, n=20) for 10 weeks. Peritoneal injection of D-Gal (700 mg/kg body weight) and LPS ($10{\mu}g/kg$ body weight) was applied to induce acute liver injury, and liver tissue was harvested 6 hours after the injection. Hematoxylin and Eosin (H&E) staining was used for liver histology. Real-time PCR was used to quantify expression of pro-inflammatory and anti-inflammatory genes in the liver. RESULTS: The liver histology showed that D-Gal/LPS treatment resulted in hepatic damage and increased number of neutrophils in conjunction with upregulation of hepatic IL-6 and $TNF-{\alpha}$ mRNAs and downregulation of hepatic $PPAR{\alpha}$ and SIRT1 mRNAs. On the other hand, the 10-week interval training resulted in a significant improvement in cardiorespiratory fitness assessed as run time to exhaustion on a treadmill. In addition, the interval training attenuated the D-Gal/LPS-induced liver damage and increased number of neutrophil in conjunction with downregulation of hepatic IL-6 and $TNF-{\alpha}$ mRNAs and upregulation of hepatic $PPAR{\alpha}$ and SIRT1 mRNAs. CONCLUSIONS: This study suggests that high-intensity interval training suppresses the D-Gal and LPS-induced acute liver damage and inflammatory responses.

• Journal of Ginseng Research
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• 제39권4호
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• pp.376-383
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• 2015

Background: Panax ginseng has a wide range of biological activities including anti-inflammatory, antioxidant, and immunomodulatory functions. Wild ginseng cambial meristematic cells (CMCs) were obtained from P. ginseng cambium. This study examined the protective mechanism of wild ginseng CMCs against $\small{D}$-galactosamine (GalN)-induced liver injury. GalN, a well-known hepatotoxicant, causes severe hepatocellular inflammatory damage and clinical features similar to those of human viral hepatitis in experimental animals. Methods: Hepatotoxicity was induced in rats using GalN (700 mg/kg, i.p.). Wild ginseng CMCs was administered orally once a day for 2 wks, and then 2 h prior to and 6 h after GalN injection. Results: Wild ginseng CMCs attenuated the increase in serum aminotransferase activity that occurs 24 h after GalN injection. Wild ginseng CMCs also attenuated the GalN-induced increase in serum tumor necrosis factor-${\alpha}$, interleukin-6 level, and hepatic cyclooxygenase-2 protein and mRNA expression. Wild ginseng CMCs augmented the increase in serum interleukin -10 and hepatic heme oxygenase-1 protein and mRNA expression that was induced by GalN, inhibited the increase in the nuclear level of nuclear factor-kappa B, and enhanced the increase in NF-E2-related factor 2. Conclusion: Our findings suggest that wild ginseng CMCs protects liver against GalN-induced inflammation by suppressing proinflammatory mediators and enhancing production of anti-inflammatory mediators.

• 자원환경지질
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• 제36권5호
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• pp.387-389
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• 2003

한반도지역에서 중력 기준점 설치, 기 설치된 중력 기준점의 중력 값들의 정밀도 향상 및 중력가속도 값을 얻기 위하여, 표준연구원(KRISS)은 한 중 공동으로 1996년 10월8일∼11월8일까지, 국립지리원(NGI)은 한$.$일공동으로 1999년 12월10∼16일까지 각각 대전과 수원에서 절대 중력계(Laser Interferometry Absolute Gravimeter type NIM-II와 FG5 Absolute Gravity meter(#203))를 사용하여 절대중력관측을 수행하였다. 표준연구원과 국립지리원이 관측한 절대 중력 값은 각각 979,829.609${\pm}$006 mGal(대전), 979,918.775${\pm}$0.0001 mGal(수원)이다. 앞으로 한반도에서 지구과학분야 활성화를 위해서 더 많은 절대중력 기준점의 확보가 필요하다고 사려된다.

• 대한원격탐사학회지
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• 제33권3호
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• pp.267-274
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• 2017

본 연구는 지표면 아래 존재할 수 있는 싱크홀 탐지를 위해 포텐셜 필드법 중 중력학적 방법을 적용하고, 그 가능성을 제시하는데 목적을 둔다. 싱크홀을 대신하여 지표면 아래 1.8 m 깊이에 수조($1.8{\times}0.8{\times}0.8m$)를 매설하였다. 매설된 위치를 중심으로 $12{\times}12m$ 구역에서 1 m 간격으로 Scintrex사의 CG-5 상대중력계(정밀도 0.001 mGal)를 이용하여 중력을 측정하였다. 싱크홀 모형에 대한 이론상 중력효과를 계산하기 위해 3차원 중력모델링을 수행하였다. 그 결과, 중력 측정에 의해 직접 산정된 싱크홀 모형에 대한 중력효과 0.036 mGal은 3차원 중력모델링에 의한 결과 0.024 mGal와 근소한 차이를 보였다. 이것은 본 연구를 위해 사용된 수조 크기와 비슷한 규모의 싱크홀은 상대중력측량에 의해 탐지될 수 있으며, 보다 조밀한 간격으로 중력탐사를 수행한다면 보다 작은 규모의 싱크홀 탐지에도 중력탐사가 충분히 적용 가능할 것으로 판단된다.