• Title/Summary/Keyword: gRPC

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Separation and Purification of Angiotensin I-converting Enzyme Inhibitory peptide from Mackerel (고등어 유래 항고혈압 peptide의 분리 정제)

  • DO Jeong-Ryong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.2
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    • pp.153-157
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    • 2000
  • Hydrolysate which inhibit the Angiotensin I-converting enzyme (ACE) was prepared from mackerel muscle by pretense. The ACE inhibitory activity of mackerel muscle hvdrolysate (MMH) was $967 {\mu}g of IC_(50)$. ACE inhibitory peptides were isolated by ultrafiltration, gel permeation column chromatography (GPC), reversed phase column chromatography(RPC), reversed phasehigh performance liquid chromatography (RP-HPLC), and gel permeation high performance liquid chromatography (GP-HPLC) from the MMH. The amino acid sequence of the ACE inhibitory peptides was Tyr-Val-Ala. The $IC_(50)$ of this peptide for ACE from rabbit lung was $1.4 {\mu}M$.

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Anticancer Protein from King Cobra(Ophiophagus hannah) and Mechanism of Action

  • Ahn, Mi-Young;Lee, Byung-Mu;Park, Ho-Koon;Shik, Kim-Yeong
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1995.04a
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    • pp.98-98
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    • 1995
  • 독사 또는 곤충의 독 30여종을 대상으로 SNU-1 위암세포에 대하여 MTT test를 실시한 결과 세포 독성 활성이 제일 높은 킹코브라(Ophiophagus hannah)의 venom을 가지고 세포 독성 물질을 정제하였다. Gel Filtration Chromatography와 Anion Exchange Chromatography로 정제한 4번째 peak만이 MTT/SRB test결과 IC$_{50}$ value 0.947$\mu\textrm{g}$/ml이었다. 음이온 교환 크로마토그라피로 정제한 단백질을 PRO-RPC로 더 분리하여 순수한 단일성분을 얻었으며 맹장암, 대장암, melanoma, fibrosarcoma 세포에 대해 독성을 확인하였고, 광학 및 전자 현미경에 의해 암세포의 분화와 성장이 억제됨을 재확인하였다. 또한 thymidine uptake asaay에서 암세포의 증식이 억제되었고, 또한 EDTA, $Zn^{++}$, $Ba^{++}$ 첨가로 세포 독성 활성이 증가되었다. (중략)

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A Study on the System for AI Service Production (인공지능 서비스 운영을 위한 시스템 측면에서의 연구)

  • Hong, Yong-Geun
    • KIPS Transactions on Computer and Communication Systems
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    • v.11 no.10
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    • pp.323-332
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    • 2022
  • As various services using AI technology are being developed, much attention is being paid to AI service production. Recently, AI technology is acknowledged as one of ICT services, a lot of research is being conducted for general-purpose AI service production. In this paper, I describe the research results in terms of systems for AI service production, focusing on the distribution and production of machine learning models, which are the final steps of general machine learning development procedures. Three different Ubuntu systems were built, and experiments were conducted on the system, using data from 2017 validation COCO dataset in combination of different AI models (RFCN, SSD-Mobilenet) and different communication methods (gRPC, REST) to request and perform AI services through Tensorflow serving. Through various experiments, it was found that the type of AI model has a greater influence on AI service inference time than AI machine communication method, and in the case of object detection AI service, the number and complexity of objects in the image are more affected than the file size of the image to be detected. In addition, it was confirmed that if the AI service is performed remotely rather than locally, even if it is a machine with good performance, it takes more time to infer the AI service than if it is performed locally. Through the results of this study, it is expected that system design suitable for service goals, AI model development, and efficient AI service production will be possible.

A Study on Web based Integration of Design Resources with a Knowledge Based Engineering Technique (KBE 기법이 적용된 설계 자원 웹 기반 통합에 관한 연구)

  • Kim J.G.;Lee S.H.;Chun H.J.
    • Korean Journal of Computational Design and Engineering
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    • v.10 no.1
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    • pp.1-10
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    • 2005
  • This paper presents an embodiment of design integration framework corresponding to changes in the latest manufacturing environment. The embodied system is named by 'WEB-KBE System' because it supports a product design with a KBE technique based on web environment. The final purpose of the work is to implement a web-based integration design environment with a KBE technique to support non-skillful designers. The framework of the system Is designed to support necessary items in user-centric design environment. Two case studies were applied to the WEB-KBE system to evaluate the efficiency, flexibility, extensibility, and reusability of the system. The examples are [1] CART integration design environment construction and (2) Exhaust Duct Saddle Support integration design environment construction. In the former case, it took a period of 8 months for modeling and implementation of the WEB-KBE prototype system. However, with the high extensibility and reusability of WEB-KBE system, the second case required only a period of one month for modeling and implementation of the system. We conclude that the presented WEB-KBE system can bring fair effects on implementing a knowledge based design environment in aspect of time and expense.

A High Precision Pulsed Field Magnetometer for Magnetic Properties Measurements of Rare Earth Magnets (희토류 영구자석의 자성측정을 위한 고감도 펄스마그네토미터)

  • Kim, Y.B.;Kapustin, G.A.
    • Journal of the Korean Magnetics Society
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    • v.15 no.4
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    • pp.250-255
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    • 2005
  • A 8 MA/m-class pulsed field magnetometer has been constructed by composing a pulsed field magnet, a pickup coil, analog integrators, a digital storage oscilloscope and a personal computer. For precision measurements, a 3-axis compensation principle has been applied for the fabrication of pickup coil, and the compensation level of the order of $10^{-6}$ and the sensitivity of $5{\cdot}10^{-7}\;Am^2$ for magnetic moment have been obtained. The high sensitivity of the magnetometer is good enough for measurements of magnetic properties of rare earth magnets in small size or thin films shorter than $3\;mm{\phi}$ in diameter.

Edible Films from Protein Concentrates of Rice Wine Meal (주박 단백질 농축물로부터 가식성필름의 제조)

  • Cho, Seung-Yong;Park, Jang-Woo;Rhee, Chul
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1097-1106
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    • 1998
  • Biodegradable edible films were prepared from rice protein concentrates (RPC) made from rice wine meal by alkaline extraction and isoelectric precipitation. The effect of film forming solution pH and plasticizers were studied, and cross-linkers were added to improve mechanical properties and water vapor permeabilities (WVP) of films. Films could be formed within pH $8{\sim}11$ with tensile strength (TS) of 4.3{\sim}5.7\;MPa$. Films produced under pH 11 had the highest TS (5.7 MPa) and the lowest WVP $(0.44\;ng{\cdot}m/m^2{\cdot}s{\cdot}Pa)$. Added glycerol, polyethylene glycol 200 (PEG) and its mixture (GLY:PEG=50:50) as plasticizers also affected the mechanical properties and WVP of films. TS and elongation at break (E) of films at various plasticizer levels were $5.5{\sim}1.0\;MPa$ and $3.6{\sim}24.3%$, respectively. At the same plasticizer concentration, the highest TS was observed when glycerol was used whereas the highest E was measured when mixture was used as plasticizer. WVPs of films with thickness of $60\;{\mu}m$ were $0.39{\sim}0.54\;ng{\cdot}m/m^2{\cdot}s{\cdot}Pa$. WVP of films decreased as the ratio of glycerol/PEG 200 was decreased, and WVP increased as the total amount of plasticizer added to the films increased. Film strength was improved by the addition of small amount of sodium hydrogen sulfate, succinic anhydride, ascorbic acid and citric acid, whereas TS of films containing $0.5{\sim}2.0%$ of NaCl and $CaCl_2$ were lower than those without the salts. The highest TS (6.3 MPa) was achieved with films containing 0.1% of succinic anhydride.

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Analysis of Broken Rice Separation Efficiency of a Laboratory Indented Cylinder Separator

  • Kim, Myoung Ho;Park, Seung Je
    • Journal of Biosystems Engineering
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    • v.38 no.2
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    • pp.95-102
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    • 2013
  • Purpose: Using a laboratory indented cylinder separator, broken rice separation experiments were conducted and the characteristics of the separation process were studied to provide information for developing a prototype indented cylinder broken rice separator. Methods: Rice (Ilmi variety) milled in a local RPC was used for the experiment. Rice kernels were classified into four groups according to their length l; whole kernels (I > 3.75 mm), semi-whole kernels (2.5 < I < 3.75 mm), broken kernels (1.75 < I < 2.5 mm), and foreign matters (I < 1.75 mm). A laboratory grain cleaner, Labofix '90 (Schmidt AG, Germany) was used for the experiments. Experiments were designed as a $4{\times}4$ factorial arrangement in randomized blocks with three replications. Cylinder rotational speeds (17, 34, 51, 68 rpm) and trough angles (15, 37.5, 60, $82.5^{\circ}$) were the two factors and feed rates (25, 50 kg/h), indent shapes (Us, $S_1$ type), and indent sizes (2.5, 3.75 mm) were treated as the blocks. Two 125 g samples and one 125 g sample were taken at the cylinder outlet and from the trough, respectively. The whole, semi-whole, and broken kernel weight ratio of the samples and feed was determined by a rice sizing device. From these weight ratios, purities, degrees of extraction and coefficient of separation efficiency were calculated. Results: Trough angle, cylinder speed, and their interaction on the coefficient of separation efficiency were statistically significant. Cylinder speed of 17, 34, and 51 rpm made the most effective separation when the trough angle was $15^{\circ}$ or $37.5^{\circ}$, $60^{\circ}$, and $82.5^{\circ}$, respectively. Maximum values of coefficient of separation efficiency were in the range of 60 to 70% except when the indent size was 2.5 mm and were recorded for the combinations of low cylinder speed (17 rpm) with medium trough angle ($37.5^{\circ}$ or $60^{\circ}$). Indent shape did not appear to make any noticeable difference in separation efficiency. Conclusions: Due to the interaction effect, the trough angle needs to be increased appropriately when an increase in cylinder speed is made if a rapid drop of effectiveness of separation should be avoided. In commercial applications, $S_1$ type indents are preferred because of their better manufacturability and easier maintenance. For successful separation of broken kernels, the indent size should be set slightly bigger than the actual sizes of broken kernels: an indent size of 3.0 mm for separating broken kernels shorter than 2.5 mm.

Composition in Milling Recovery Ratio of Rice Cultivars, Ilpumbyeo and Chucheongbyeo (일품벼와 추청벼의 도정률 차이의 작물학적 요인분석)

  • Kim, Deog-Su;Kim, Sun-Lim;Song, Jin;Hur, On-Suk;Kim, Jung-Tae;Lee, Choon-Ki;Kim, Jae-Hyun;Kim, Kee-Jong;Suh, Sae-Jung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.3
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    • pp.308-313
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    • 2008
  • This study was carried out to analysis the factor effected by milling rice rate, and to provide the developing rice varieties and cultivation technology. Panicle numbers per spike of Ilpumbyeo and Chucheongbyeo were 105 and 70, respectively. The primary branch panicle rate was Ilpumbyeo 56.2% and Chucheongbyeo 61.4%. The secondary panicle rate of Ilpumbyeo and Chucheongbyeo was 43.3% and 37.9%, respectively. Grain filling rate using specific gravity showed that Ilpumbyeo was the lower filling rate than Chucheongbyeo. Hull weight per one grain was Ilpumbyeo 41.9 mg and Chucheongbyeo 3.92 mg, and hull weight per rough rice 1 kg was Ilpumbyeo 157.36 g and Chucheongbyeo 151.31 g. In milling rate, brown rice ratio by ripening degree of llpumbyeo and Chucheongbyeo with combine harvest was 81.22% and 82.52%. Milled/brown rice ration of llpumbyeo and Chucheongbyeo showed 92.14% and 92.51%. The milling recovery ratio of llpumbyeo was decreased 1.67% then Chucheongbyeo, and the difference was mainly due to the hull weight (0.61%), milled/brown rice ratio (0.37%) and ripening degree (0.69%). Although the varietal differences were found in hull weight and millied/brown rice ratio between llpumbyeo and Chucheongbyeo, the ripening degree was considered as the factor that could be reduced by cultivation technology and post-harvest management.

Basic characteristics of an indented cylinder broken rice separator (원통형 홈 선별기의 쇄미선별 특성)

  • 순영석;김명호;박승제;이종호
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2002.02a
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    • pp.282-288
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    • 2002
  • 본 연구는 상업용 규모의 쇄미선별공정에 사용할 수 있는 파이로트 규모 원통형 홈 선별기 시작기의 설계 및 제작에 필요한 기초 연구로서, 실험실용 원통형 홈 선별기를 이용하여 쇄미선별 실험을 실시하였다. 원통 회전속도, trough각도, indent 크기와 형상, 공급량을 요인으로 하여 완전립의 수거율과 순도, 쇄립의 수거율과 순도 및 선별효율을 조사하였다. 수행한 연구결과를 요약하면 다음과 같다. 1. 선별효율에 대한 원통 회전속도와 trough각도의 단독효과 및 교호작용은 1% 수준에서 유의성이 인정되었다. 원통 회전속도가 커지면trough각도 역시 그에 따라 적절히 증가시켜야만 선별효율의 저하가 방지되는 것으로 나타났다. 2. 최고 선별효율 값은 홈의 모양과 크기, 그리고 공급량에 관계없이 낮은 회전속도 (17rpm)와 중간 trough각도 (37.5$^{\circ}$또는 60$^{\circ}$)가 조합된 처리에서 나타났으며, 60~70% 범위의 높은 값을 보였다. 선별효율에 관한 원통 회전속도와 trough각도의 최적 조합은 17rpm, 37.5$^{\circ}$라고 판단된다. 3. 말발굽형 홈과 반구형 홈 간 선별효율의 차이는 없었다. 따라서, 실제 상업용 규모의 원통형 홈 쇄미선별기 개발에 있어서는 제작이 쉽고 유지.보수가 간편한 반구형 홈을 채택하는 것이 바람직할 것으로 생각된다. 길이 2.5mm이하의 미립인 쇄미의 선별에 사용할 홈의 크기는 2.5mm 보다는 약간 큰 3.0mm 정도가 되어야만 할 것으로 판단된다. 4. 공급량에 따른 선별효율의 차이는 1% 수준에서 유의성이 인정되었으며, 공급량이 작았을 때 전반적으로 선별효율이 높았다.타리 시프터를 채택, 사용하고 있었으며, 로타리시프터 사용상의 문제는 회전몸체를 지지하는 rod spring의 파손 등 구조와 관련된 것이었다. 로타리 시프터에 의한 쇄립의 선별과 제거정도는 만족할 만한 수준은 아니었다. 4. 국내 유통백미 완전립의 길이, 폭, 두께는 각각 5.02mm, 2.93mm, 2.03mm이었으며, 산물밀도와 천립중은 각각 745.3kg/m3 및 20.46g이었다. 5. RPC 백미제품의 품질경쟁력 향상을 유도하고자 현행 쇄미의 정의와 기준을 보다 강화하여 다음과 같은 쇄미 기준과 계급을 설정, 제시하였다. "완전립" - 길이가 3.75mm이상인 미립 "준완전립" - 길이가 2.5∼3.75mm인 미립 "쇄미" - 길이가 1.75∼2.5 mm인 미립 "이물" - 길이가 1.75mm이하인 미립.볼 때 상토 종자혼합비 6 : 1, 성형롤 회전속도 약 7 rpm으로 판단되며, 이 때 제조능력은 시간당 약 65 Kg(펠렛종자 약 39,000 개), 성형률 약 87 %, 종자손실률은 약 30 %, 펠렛종자 내 평균 종자수는 약 5.5 개, 완전 벼 종자 3개 이상 포함 펠렛종자 비율은 약 100 %가 될 것으로 보인다. 세포의 Androgen 생성을 촉진시키는 역할이 있는 것으로 보여진다 따라서 옻나무 유래 F는 포유동물의 생식기능에 중요하게 작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성 물질로 증착시키지 않고, Silver Paint 에 시편을 접착하는 방법으로도 만족한 시험 결과를 얻을 수 있었다.째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간

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Mediation of Production Trust on Brand Image Influence on Repurchase Intention for PB Rice (브랜드 이미지와 구매의도 간의 영향관계에서 상품 신뢰의 매개효과 검증: PB 쌀을 중심으로)

  • Kim, Deok-Hyeon;Ha, Ji-Young;Lee, Seung-Hyun;An, Wook-Hyun
    • Journal of Distribution Science
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    • v.12 no.8
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    • pp.83-90
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    • 2014
  • Purpose - Increasing sales of PB rice products can hinder the growth of domestic brands of rice, notwithstanding that the government is promoting domestic brands of rice. This analysis evaluated the influencing relationship among the variables of PB image, product credibility, and purchase intention of consumers who have never bought PB rice, to know the factors influencing consumers' decisions to purchase PB products. Research design, data, and methodology - PB products' brand image was analyzed as the factor that has a direct effect on purchase intention. The mediation effect of credibility on PB products is also analyzed, in terms of influencing the relationship between PB products' brand image and purchase intention. The analysis is performed on consumers that have not purchased a PB product and consumers who have purchased PB products from major distributors. Data is collected through questionnaires, from 389 of responders, and the AMOS 19.0 statistics package is used as a statistical tool. Results - It is proven that brand image has direct effects on the credibility of the product, and the credibility of product has a direct effect on purchase intention. However, it is revealed that brand image does not directly affect purchase intention, but that brand image indirectly affects it through the credibility of the product. Although the customers' recognition about PB image is low, the result shows that PB products' external attributes have effects on customers' purchase intention relating to the PB product. Therefore, it is important to establish the credibility of the PB product more than other products in terms of marketing. Conclusions - The following are the implications of the study. First, in a rice brand promotion, the credibility of the product should be ensured by a uniform brand image. To ensure the credibility of a product, the RPC brand and other brands should be unified, and the unified brand image should be applied to every product. Second, the package must possess a design as well as contents that could build consumers' perception of product credibility. Products' external attributes contribute to their credibility, which leads the consumers to purchase the products, including those consumers who have never bought PB rice products. Therefore, the products' credibility and sales can be reinforced by applying information about consumers' considerations when buying the PB rice product in different colors, font sizes, and packaging designs. This study is meaningful in two ways. First, it seeks to identify an NB revitalization strategy by exploring the purchasing behavior of customers who have no experience in buying PB rice products. Second, the results of previous studies about general brands are considered and applied in this study in order to investigate the influencing relationship among different factors of PB products. However, this study is a consumer awareness investigation; therefore, its results only have limited meaning to the relationship between brand image and purchase intention.