• Korean Journal of Environmental Agriculture
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• v.24 no.4
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• pp.350-357
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• 2005

This study was conducted to investigate the downward mobility of pesticides using soil columns and to compare the experimental results with values predicted from Convective mobility test model. Nine pesticides such as metolcarb, molinate, fanobucarb, isazofos, diazinon, fenitrothion, dimepiperate, parathion and chlorpyrifos-methyl were used for leaching test in soil column for four soils; Jungdong (upland soil), Gangseo (paddy soil), Yesan (forest soil), and Sineom(upland, volcanic ash-derived soil) series. The peak concentrations leached from 10 cm-columns of three soils except Sineom series ranged 6.5 to 12.6 mg/L for metolcarb, 2.6 to 5.0 mg/L for molinate, 4.5 to 7.8 mg/L for fenobucarb, 0.39 to 1.36 mg/L for dimepiperate, 1.1 to 4.6 mg/L for isazofos, 0.01 to 0.14 mg/L for diazinon, lower than 0.01 to 0.70 mg/L for fenitrothion and lower than 0.01 to 0.44 mg/L for parathion. But chlorpyrifos-methyl was not leached from any soil columns. Elution volumes to reach the peak of metolcarb, molinate, fenobucarb, isazofos, diazinon, and dimepiperate in the leachate ranged 1.1 to 2.1 pore volume (PV), 1.6 to 3.3 PV, 1.6 to 3.3 PV, 2.1 to 4.4 PV, 6 to 15 PV, and 8 to 21 PV, respectively. On the same water flux conditions, convection times estimated by Convective mobility test model were coincided with results from soil column test in most of the soil-pesticide combinations applied. Based on convection times estimated by the model at standard conditions (water flux 1 cm/day), metolcarb was classified as most mobile, molinate, fenobucarb and isazofos as mobile or most mobile, dimepiperate as moderately mobile or mobile, diazinon as mobile, fenitrothion and parathion as slightly mobile or mobile and chlorpyrifos-methyl as immobile or slightly mobile.

• Korean Journal of Microbiology
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• v.34 no.3
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• pp.154-161
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• 1998

To investigate whether the introduced genetic marker is useful to detect the survivalship and activity of the natural bacteria under the stressed conditions, one Gram-negative isolate, KP964 was transformed to the luminous phenotype by transferring luxAB gene. Under the starvation-stress this luminous bacterial culturability (determined by colony-forming-units [CFU] on agar plate) decreased rapidly below the detection limit by 37 days, while its total cell number (determined by AODC) remained almost the same as its initial inocular size. At that time period, the viable cell number was estimated to be 1400 times higher than its CFU number. The bioiuminescence (determined by relative light units [RLU]) produced under the same condition was also monitored and found to decrease more rapidly than the culturability by 5-fold. Under the other stresses, e.g., osmotic shocks, acid shock, and exposure to toxic chemicals, this bacterial strain did not show the reliable correlation between CFU and RLU. These results might not suggest the direct estimation of bioiuminescence from the stressed bacteria be an index of both the survivalship and its activity. However, when the stressed bacterial cells were incubated under the favorable condition by relieving from the existing stress, the potential bioiuminescence (the lag periods before the increase of bioiuminescence, the increase rates of bioiuminescence, and the maximal levels of bioiuminescence) was shown to be highly dependent upon the strengths of the stresses exposed to the bacterial cells. Therefore, analysis of the potential bioiuminescence from the stressed bacteria revealed good relationships with survival as well as activity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.12
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• pp.4863-4872
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• 2010

This study examined the disease conditions of patients from a farming and fishing village area. In order to analyze the medical service utilization, the necessary data were obtained from established health and medical care service plans from medical treatment related organizations. The following results were based on the analysis of data from the medical records of 2,365 discharged patients during a six months period from July to December 2009 at a general hospital in an aging farming and fishing village area. Results: The sex of the patients investigated was male 55.3%, female 44.7%, and the most frequent age category at 42.0% was 70 years of age or older. Based on type of hospital admission, 65.5% of patients who were admitted were originally outpatients. Patients were admitted according to the following departments: 49.7% for the department of internal medicine, 16.7% for the department of orthopedics, and 13.8% for the department of neurosurgery. The average number of days hospitalized was 14.8 days. The following ranks the principal diagnosis among patients in this study: S00-T98 18.4%, J00-J99 15.5%, and I00-I99 11.5%. The average number of diagnosis listed per patient was 5.6. There was a statistically significant difference for the following general characteristics according to principal diagnosis list: gender, type of insurance, admission process, and age category distribution had statistically significant differences. Monthly distribution of principal diagnoses were statistically significant difference. There was a statistically significant difference for principal diagnosis lists according to the average number of days admitted and the number of diagnosis. The results of this study showed the types of disease from typical farming and fishing village regions as disease from external injury due to the work environment of farming and fishing village regions and excessive labor throughout the year, respiratory disease, and various chronic disease from aging.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.2
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• pp.156-164
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• 2015

High temperature stress would affect rice production in the future as heat wave is expected to occur frequently under climate change conditions. The objective of this study was to obtain rudimentary information to assess the impact of heat stress on rice yield and its yield component in Korea. Two rice cultivars "Hwaseongbyeo" (Japonica) and "Dasanbyeo" (Tongil-type) were grown at different nitrogen fertilization levels in two seasons. These cultivars were grown in 1/5000a Wagner pot placed within four plastic houses where temperature was controlled at ambient, ambient$+1.5^{\circ}C$, ambient$+3^{\circ}C$ and ambient$+5^{\circ}C$ throughout the rice growing season in Suwon ($37^{\circ}16^{\prime}N$, $128^{\circ}59^{\prime}E$), Korea. The degree of temperature change affected grain yield whereas the level of nitrogen had little impact on grain yield. The number of panicle per pot and spikelet per panicle were not significantly different among temperature treatments in both cultivars tested. In contrast, 1000-grain weight and ripened grain ratio were decreased significantly under the treatments raising the air temperature to the level of $5.0^{\circ}C$ and $1.5^{\circ}C$ above the ambient air temperature in Dasanbyeo and Hwaseongbyeo, respectively. Reduction of 1000-grain weight and ripened grain ratio under the temperature treatments of $3.0^{\circ}C$ and $5.0^{\circ}C$ above the ambient air temperature resulted in significantly less grain yield for Dasanbyeo and Hwaseongbyeo, respectively. The greater sensitivity of grain yield to temperature increase in Dasanbyeo was attributable to the sharp decrease of 1000-grain weight and ripened grain ratio with the temperature rise above $23^{\circ}C$ during ripening period. On the other hand, Hwaseongbyeo had little variation of them in the temperature range of $23-27^{\circ}C$. These results suggested that grain yield would decrease under future climate conditions due to grain weight decreased by shorter grain filling period as well as the ripened grain ratio reduced by spikelet sterility and early abortion of rice kernel development. Thus, it would be essential to use cultivars tolerant to heat stress for climate change adaptation, which merits further studies for developing varieties that have traits to avoid spikelet sterility and early abortion of rice kernel, e.g., early morning flowering, under heat wave.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.6
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• pp.814-819
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• 2004

An investigation was carried out to study the effect of two housing systems on feed intake and nutrient utilization of sheep in a semi-arid region of India. Two types of housing managements were adopted. The first was a shed- 20'${\times}$10' structure with all the four sides of 6' chain link fencing with central height of 10'. The roof was covered with asbestos sheets, with mud floorings. The second was an open corral- 20'${\times}$10' open space with all the four sides covered with 6' chain link fencing. Thirty-four (32 ewes and 2 rams) sheep were grazed together on a 35 ha plot of native range. All the sheep were grazed as a flock from 08:00 to 17:00 h during the yearlong study. The flock was divided into two groups (16 ewes+1 ram) in the evening and housed according to two housing systems (Shed and Open Corral). Three digestion trials were conducted during three defined seasons of monsoon, winter and summer seasons to determine the effect of housing on nutrient intake and utilization. Blood samples were collected in three seasons for the estimation of hemoglobin and glucose. Dry and wet bulb temperatures were recorded at 06:00 A.M. and 09:00 P.M. using suitable thermometers both inside the shed and in the open corral and temperature humidity index (THI) was calculated. There was significant (p<0.05) difference in the THI between shed and open corral in all the seasons, indicating that the shed was always warmer compared to open corral. The daily dry matter intake (DMI, g/d) was 965, 615 and 982 in sheep housed under shed and 971, 625 and 1,001 in those housed in open corral during monsoon, winter and summer season, respectively. These differences were however non-significant (p>0.05). The digestibility of DM was 45.92, 45.13 and 50.30 in sheep housed under shed and 43.64, 45.02 and 55.02 in sheep housed in open corral during monsoon, winter and summer seasons, respectively. There was no significant (p>0.05) difference in the digestibility of nutrients in sheep maintained under shed and in open corral. Blood Hb concentration was 13.97, 14.13 and 13.15 in sheep housed under shed and 15.27, 13.63 and 14.82 in those kept in open corral, whereas blood glucose concentration was 59.67, 59.70 and 52.33 in sheep under shed and 61.00, 61.00 and 57.83 in open corral, during monsoon, winter and summer, respectively. There was also no significant effect of housing on the body weight changes, wool yield and survivability in ewes. Although housing had no significant effect on nutrient intake, their utilization and blood parameters, there was significant effect on the physiological responses and energy expenditure of sheep maintained under the two housing systems (Bhatta et al., 2004). It can be concluded from this study that the housing systems didn't have any significant effect on the nutrient intake and utilization of native breed like Malpura, which were well adapted to the hot semi-arid conditions of India. However, while deciding provisions for housing of different breeds of sheep (both crossbred and native) parameters like physiological responses, energy expenditure, health conditions and overall economics of the systems should be taken into consideration.

• The Korean Journal of Pesticide Science
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• v.6 no.4
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• pp.300-308
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• 2002

This study was conducted to investigate the downward mobility of pesticides using soil colunms and to compare the experimental results with predicted values from Convective mobility test model. Five pesticides including ethoprophos, procymidone, iprobenfos, isoprothiolane, and butachlor were subjected to soil column leaching test for three types of cultivation soils. The concentrations of ethoprophos, iprobenfos, procymidone, isoprothiolane and butachlor leached from soil column of 30 cm depth ranged $0.74{\sim}3.61mg/mL,\;0.36{\sim}1.67mg/L,\;0.16{\sim}0.84mg/L,\;0.16{\sim}0.67mg/L$ and lower than 0.15 mg/L, respectively. Elution volume to reach the peak of ethoprophos, iprobenfos, procymidone, isoprothiolane and butachlor in the leachate ranged $2{\sim}4PV,\;3{\sim}10PV,\;5{\sim}13PV,\;4{\sim}14PV\;and\;19{\sim}61PV$, respectively. Convection times predicted by Convective mobility test model at standard conditions were $9{\sim}18$ days for ethoprophos, $17{\sim}35$ days for iprobenfos, $24{\sim}54$ days for isoprothiolane, $21{\sim}65$ days for procymidone and $105{\sim}279$ days for butachlor. Based on these convection times, ethoprophos was classified as mobile or most mobile, isoprothiolane and procymidone as moderately mobile or mobile and butachlor as slightly mobile. On the same conditions, convection times from the model were coincided with those from soil column test in most of the soil-pesticide combinations applied. Therefore, Convective mobility test model could be applied to predict convection times of pesticides.

• Clinical and Experimental Reproductive Medicine
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• v.35 no.4
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• pp.247-265
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• 2008

Objectives: Many types of liver diseases can damage regenerative potential of mature hepatocytes, hepatic progenitor cells or oval cells. In such cases, a stem cell-based therapy can be an alternative therapeutic option. We examined whether human amnion-derived mesenchymal stem cells (HAM) and human umbilical cord-derived stem cells (HUC) could differentiate into hepatocyte-like cells as therapeutic cells for the liver diseases. Methods: HAM and HUC were isolated from the amnion and umbilical cord of the volunteers after a caesarean section with informed consent. In order to differentiate these cells into hepatocyte-like cells, cells were cultivated in hepatogenic medium using culture plates coated with fibronectin. Effects of hepatocyte growth factor, L-ascorbic acid 2-phosphate, insulin premixture fibroblast growth gactor 4, dimethylsulfoxide, oncostatin M and/or dexamethasone were examined on the hepatic differentiation. After differentiation, the cells were analyzed by RT-PCR, immunocytochemistry, immunoblotting, albumin ELISA, urea assay and periodic acid-schiffs staining. Results: Initial fibroblast-like appearance of HAM and HUC changed to a round shape during culture in the hepatogenic medium. However, in all hepatogenic conditions examined, HUC secreted more amounts of albumin or urea into medium than HAM. Expression of some of hepatocyte-specific genes increased and expression of new genes were observed in HUC following cultivation in hepatogenic medium. Results of immunocytochemistry and immunoblotting analyses demonstrated that HUC secreted albumin into the culture medium. PAS staining further demonstrated that HUC could store glycogen inside of the cells. Conclusions: Both HUC and HAM could differentiate into albumin-secreting, hepatocyte-like cells. Under the same hepatogenic conditions examined, HUC more efficiently differentiated into hepatocyte-like cells compared with the HAM. The results suggest that HUC and HAM could be used as sources of stem cells for the cell-based therapeutics such as in liver diseases.

• Journal of the Korean Geographical Society
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• v.41 no.5 s.116
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• pp.527-544
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• 2006

This study provides a definition of heat waves, which indicate the conditions of strong sultriness in summer, appropriate to Korea and intends to clarify long term(1973-2006) averaged spatial and temporal patterns of annual frequency of heat waves with respect to their intensity. Based on examination of the Korean mortality rate changes due to increase of apparent temperature under hot and humid summer conditions, three consecutive days with at least $32.5^{\circ}C,\;35.5^{\circ}C,\;38.5^{\circ}C,\;and\;41.5^{\circ}C$ of daily maximum Heat Index are defined as the Hot Spell(HS), the Heat Wave(HW), the Strong Heat Wave(SHW), and the Extreme Heat Wave(EHW), respectively. The annual frequency of all categories of heat waves is relatively low in high-elevated regions or on islands adjacent to seas. In contrast, the maximum annual frequency of heat waves during the study period as well as annual average frequency are highest in interior, low-elevated regions along major rivers in South Korea, particularly during the Changma Break period(between late July and mid-August). There is no obvious increasing or decreasing trend in the annual total frequency of all categories of heat waves for the study period However, the maximum annual frequencies of HS days at each weather station were recorded mainly in the 1970s, while most of maximum frequency records of both the HW and the SHW at individual weather stations were observed in the 1990s. It is also revealed that when heat waves occur in South Korea high humidity as well as high temperature contributes to increasing the heat wave intensity by $4.3-9.5^{\circ}C$. These results provide a useful basis to help develop a heat wave warning system appropriate to Korea.

• Journal of Sericultural and Entomological Science
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• v.2
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• pp.49-61
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• 1962

The insect Diplosis mori Yokoyama is causing extensive destruction of mulberry trees in Korea with a resultant loss in silk production. This study was made to determine an effective method of control. Methods and Materials Used Preliminary studies were made to determine more exactly the life cycle of the insect. Based on this information, various control measures were tested, including the use of spray methods with BHC and control of larvae by tilling. Results Obtained 1. Life cycle studies (a) In the Suwon area, this-insect has 5 generations per year. The first starts in the later part of June and the final cycle ends in the later part of September. (b) The adult insects appear about 7: 00-8: 00 P.M. and live for 2-5 days. Females live in longer periods than the male. (c) Larvae lives inside the second and third stipules (A. B.) before mulberry leaf development. They cause extensive damage to the leaves at the point where they are attached to the stem. (d) Weather conditions considerably affect the life cycle. The pupa particularly are affected and not be able to change into the moth stage when there is a long period of no rain. (e) Larvae are large......0.3 to 2.0mm......and are milky-white immediately after hatching but turn to pinkish as the worm matures. The matured worm has a jumping ability up to 15-20cm. The worm burrows into the ground 1.5 to 3.0 cm before changing into the pupal stage. (f) The pupal stage usually lasts 7-8 days, in summer weather conditions and the pupa is surrounded with a coarse cocoon. (g) These insects, as a general rule, overwinter as pupae but sometimes as larvae. 2. Control measures (a) BHC dust applied on the ground seem most effective. It should be done 4-5 days after the worm has burrowed into the ground. For this control, it is recommended that 6kg of a 2% formation Tanbo(l0ares) be used. (b) For the effective spraying against the fly, it is recommended that a formulation of liquid BHC spray terials be used at the rate of 400-600 liters per Tanbo. (c) Tillage methods which provide a cover of soil 5cm or more in depth above infested areas will effect-maively prevent the emergence of the fly from the pupal stage. 3. Conclusions Methods of control against Diplosis mori Yokoyama can be tied more closely to the life cycle of the insect with more effective results. Further studies are needed to complete information on possible controls during or after hibernation. Economic studies on the cost of these control measures are also needed.

• Korean journal of food and cookery science
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• v.20 no.4
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• pp.342-351
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• 2004

Rice flour was roasted at different temperatures and times and tarakjuk was made from these roasted rice flours. Chemical composition, in vitro starch digestibility (IVSD) and protein digestibility (IVPD) of the roasted rice flours and tarakjuk were determined. Changes in quality characteristics such as pH, viscosity, IVSD and IVPD of tarakjuk during refrigerated storage were also investigated. For roasted rice non, the protein content ranged from 6.52∼7.13% on a dry basis, while for tarakjuk, the range was 3.74 ∼4.0%. On the other hand, the Ca level of tarakjuk ranged from 1,278.36∼1,340.87 ppm. Rice flours showed decreasing IVSD and increasing IVPD on roasting at 145$^{\circ}C$ and 165$^{\circ}C$, whereas they showed increasing IVSD and decreasing IVPD at 185$^{\circ}C$, respectively. Roasting also influenced the pH, viscosity, IVSD md IVPD of tarakjuk made from these roasted rice flours. As the roasting temperature and time increased, tarakjuk showed lower pH and viscosity, however it showed higher IVSD and IVPD. The pH of tarakjuk, except that of control, decreased with storage, whereas viscosity increased significantly. IVSD decreased up to 4 days of storage, after which it increased again until 14 days of storage. On the other hand IVPD increased up to 7 days of storage, but there was no additional significant increase after 14 days of storage. These results suggest that depending on the nutritional purpose, appropriate conditions for roasting of rice flour and storage of tarakjuk may be recommended for the commercialization of tarakjuk.