• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.158-162
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• 2005

정어리에서 분리한 5개의 균주를 이용하여 antiangiogenesis 효과가 가장 뛰어난 균주를 ${\lambda}-28$이라 명명하고 이를 대량 배양하여 단백질 추출법인 염석과 투석, 동결건조를 통해 시료를 제조하여 이를 SEC를 통해 fraction별로 분리한 후 SDS-PAGE와 antiangiogenesis, cytotoxcity test를 수행하여 ${\lambda}-28$번의 76 fraction에서 control군에 비해 82.7%의 높은 antiangiogenesis 효과를 보였고, 세포독성 실험에서도 낮은 독성을 보였다.

• 한국식품조리과학회지
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• 제12권3호
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• pp.331-338
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• 1996

The main aim of this study was to develop carrot soups with fishery products, which is improved from traditional gruel of Cheju island. For this the optimal procedure and ingredient mixing ratio for making basic carrot soup was determined through the instrumental measurement, the sensory evaluation and the nutrient analysis, and then the carrot-fishery soups were prepared by mixing the fishery products from Cheju island. The results were as follows: The optimal procedure for making basic carrot soup was to saute the sliced carrots and soaked rices with the sesame oil for 5 min, add the water, bring to a boil for 6 min, simmer for 15 min, then puree the soup. The optimal ingredient mixing ratio in the basic carrot soup was carrot 300 g: rice 45 g: water 900 $m\ell$: sesame oil 15 $m\ell$: salt 5 g. For preparing carrot-fishery soups were the optimal fishery products mixing ratio i) 30% tile fish or crab, ii) prawn, abalone, top shell, or ear shell 20%, iii) sea urchin 15%, iv) fusiform or gulf weed 5％. The order of sensory evaluation scores, on a 5-point scale, were sea urchin, crab > top shell, tile fish > abalone, ear shell > prawn > coral fish > fusiform, gulf weed. Nutrient composition analysis showed that vitamin A was 5 times higher in carrot soup than in pumpkin soup. Sensory evaluation scores show that carrot soup was prepared to pumpkin soup. A portion (200 g) of the soup would provide 144% of the recommended daily allowance of vitamin A. The results of this work indicate that an acceptable carrot-fishery soups of better nutritional and sensory values than pumpkin soup can be prepared.

• 한국해양바이오학회지
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• 제1권3호
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• pp.156-162
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• 2006

The halophilic enterobacteria, Enterobacteria cancerogenus, was isolated from the intestines of the fusiform fish (Trachurus japonicus) to yield a protein-like material termed PLM-f74. PLM-f74 was characterized by strong inhibition ratios to angiogenesis (82.8% at the concentration of $18.5{\mu}g/mL$) and elevated antioxidative capacities with low toxicity. The PLM-f74 is a glycoprotein comprised of saccharides and amino acids. PLM-f74 inhibited non-activated U937 monocytic cell adhesion to HUVECs activated with IL-$1{\beta}$ by 78.0%, and the adherence of U937 cells treated with the PLM-f74 and stimulated with IL-$1{\beta}$ to unstimulated HUVECs decreased by 102%. When both cell types were pretreated with PLM-f74, the adhesion of U937 cells to IL-$1{\beta}$ stimulated HUVECs was completely suppressed by 121% at a concentration of 18.5 ug/mL. PLM-f74 blocked signal pathways from VEGFR2, PI3K, ${\beta}$-catenin and VE-cadherin to NF-kB based on western bolt analysis. And also inhibited IL-1-stimulated HUVEC expression of the adhesion molecules, ICAM-1 by 40%, VCAM-1 by 60%, and E-selectin by 70% at the same concentration noted above. New anti-angiogenic and anti-cell adhesion materials showing elevated antioxidative capacities and non-toxicity may be expected from these results.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1544-1553
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• 2006

The halophilic enterobacteria, Enterobacteria cancerogenus, was isolated from the intestines of the fusiform fish (Trachurus japonicus) to yield a protein-like material termed PLM-f74. PLM-f74 was characterized by strong inhibition ratios to angiogenesis (82.8% at the concentration of $18.5{\mu}g/ml$) and elevated antioxidative capacities with low toxicity. The PLM-f74 is a glycoprotein comprised of saccharides and amino acids. PLM-f74 inhibited cell adhesion that non-activated U937 monocytic cell adhesion to HUVECs activated with $IL-1{\beta}$ by 78.0%, and the adherence of U937 cells treated with the PLM-f74 and stimulated with $IL-1{\beta}$ to unstimulated HUVECs decreased by 102%. When both cell types were pretreated with PLM-f74, the adhesion of U937 cells to $IL-1{\beta}$-stimulated HUVECs was completely suppressed by 121% at a concentration of $18.5{\mu}g/ml$. PLM-f74 blocked signal pathways from VEGFR2, PI3K, ${\beta}$-catenin, and VE-cadherin to NF-kB, based on western bolt analysis. It also inhibited IL-l-stimulated HUVEC expression of the adhesion molecules, ICAM-l by 40%, VCAM-l by 60%, and E-selectin by 70% at the same concentration noted above. New anti-angiogenic and anti-cell adhesion materials showing elevated antioxidative capacities, and non-toxicity may be expected from these results.

• Journal of Plant Biology
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• 제26권1호
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• pp.7-16
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• 1983

Internodal vessel elements are studied in 20 genera, 75 species and 33 cultivars of Hibiscus rosa-sinensis belong to the Malvaceae. The minimum and maximum length and diameter range from 79~466 and 14~88 ${\mu}{\textrm}{m}$, respectively. The vessel elements are either cylindrical, fusiform, conical, clavate, oval, column-, drum-, fish and cup-like or erratic. Perforation plates are exclusively simple in all the species investigated, except occasionally scalariform in varieties of Hibiscus rosa-sinensis. In most of the species studied vessel elements have predominantly two perforation plates or occasionally one and three. Adjacen side wall thickening is commonly simple and border pitted, mixed, scalariform, helical and reticulate. The vessel elements vary in their size, shape, number and inclination of perforation plates and adjacent wall thickening.

• 한국어병학회지
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• 제9권1호
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• pp.21-31
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• 1996

양식산 무지개송어(Oncorhynchus mykiss) 및 한국산메기(Silurus asotus)를 시료로 하여 Ichthyophthirius multifiliis의 발달 단계를 형태학적으로 관찰하고 또한 수온별로 탈낭에 따른 소요시간을 조사하고자 하였다. 1. multifiliis의 발달단계를 형태학적으로 관찰한 결과 크게 기생기(parasitic phase)와 비기생기(non-parasitic phase)로 구분할 수 있었고 phoront, trophont, protomont, tomont, tomite 및 theront 의 6 단계의 발달 과정을 거치는 것을 알 수 있었다. 침입형인 phoront 는 $60{\times}40-100{\times}70{\mu}m$의 방추형 또는 구형으로 34-38열의 meridional kineties를 가졌고 구부 장치가 발달하기 시작하였다. 영양형인 trophont는 80-$800{\mu}m$의 구형 또는 아메바형이며, 말굽형의 대핵, 미미한 세포구 및 발달된 수축포를 가진 것으로 관찰되었다. 피낭 형성형인 protomont는 200-$800{\mu}m$의 구형으로 미미한 대핵과 현저히 발달된 수축포를 가지고 있었으며 구부 장치의 흡수 및 exocyst를 형성하기 시작하였다. 피낭체내 분열형인 tomont는 크기와 모양이 protomont와 같았으나 대핵은 보이지 않았고 endocyst를 형성하여 두꺼운 cyst wall 을 가졌으며 구부 장치가 완전히 흡수된 것으로 관찰되었다. 딸세포형인 tomite는 35-$50{\mu}m$의 구형으로 세포구가 보이며 구부장치가 나타나기 시작했다. 감염형인 theront는 크기, 모양 및 meridional kineties가 phoront와 같았으며 돌기 및 세포구가 앞끝과 중간 부위에 있는 것으로 나타났다. I. multifiliis 의 탈낭에 따른 소요 시간은 수온과 직접적으로 관련이 있는 것으로 조사되었으며 수온 $28^{\circ}C$에서 10-14 시간이 소요되었고 수온이 낮아질수록 탈낭에 따른 소요 시간이 길어져서 수온 $9^{\circ}C$에서는 약 129 시간이 소요되는 것으로 나타났다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.406-411
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• 2005

Fermented materials with enterobacteria isolated from fusiform fish, have strong anti-angiogenesis effect and anti-cell adhesion effect. PLM-f74 got from 74th fraction of size exclusion chromatography from fermented material, showed strong anti-cell adhesion effect between HUVECs and U937 monocytic cell. Adhesion of U937 cell to HUVEC stimulated with IL-1b was clearly inhibited by PLM-f74 in a dose-dependent manner by 12.1, 21.2, 50.9, and 78.2%, when U937 cells treated with each of the PLM-f74 and stimulated with PMA (100 mg/L) was added onto untreated and unstimulated HUVECs, adhesion was observed by 15.8, 31.9, 70.8, and 102%, when both cell types were pretreated with PLM-f74, the adhesion was prominently decreased by 83.7, 99.2, 110, and 120.8%, with 0.74, 3.7, 7.4, and 18.5ug/mL of PLM-f74, respectively. PLM-f74, also, reduced IL-1-stimulated HUVEC expression of adhesion molecules, VCAM-1, ICAM-1, and E-selectin dose-dependently by ELISA method.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.335-339
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• 2005

정어리에서 분리한 균주 중 가장 항산화력이 강한 균주를 5개를 선발하여 이를 antiangiogenesis test를 통하여 효과가 가장 뛰어난 균주인 ${\lambda}-28$번 균주를 선발하였다. 균주의 최적 배지를 제조하기 각기 다른 온도, 영양성분등을 투여하여 최적의 조건을 알아보았다. 온도에 있어서는 균주의 특성상 바다온도와 가장 비슷한 $25^{\circ}C$에서 가장 좋은 성장률을 보였고 영양적인 측면에서 보면 탄소원에서는 glucose가 균주 성장률이나 항산화력에서 다른 첨가물에 비해 유효한 효과를 보였고, 무기 탄소원에서 ammonium chloride가 좋은 항산화력을 보였다. 유기 탄소원을 첨가한 실험에서는 유효한 효과가 나타나지 않았다.

• 수산해양기술연구
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• 제59권3호
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• pp.189-195
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• 2023

This study quantitatively evaluated size selectivity for three netting shapes (T0; regular, T45, T90) and hanging ratio (35%, 70%) of T0 netting used for trawl codend. The size selectivity experiment was performed in a tank using a cube experimental model with a length of 50 cm on one side and 389 experimental individuals, jack mackerel (Trachurus japonicus). In the selectivity analysis, a selectivity curve was created based on the selection ratio using a logistic function, and the 25%, 50%, and 70% selection length and selection range (SR) were obtained. The T0 netting was 19.54 cm when the 50% selective length, which is a selectivity evaluation index, had a hanging ratio of 35%, a selection range of 0.51 cm, and 22.70 cm and 3.08 cm for the hanging ratio of 70%. The T45 netting was 24.34 cm and 2.13 cm, and the T90 netting was 23.51 cm and 2.84 cm. The results of the T45 netting and the T90 netting are similar, and the 50% selection length and selection range were relatively larger than the T0 netting. There was a significant difference in the correlation between the circumference of the inner circle of the mesh by the shape of the netting and the body girth of the experimental individual (Pearson test, r = 0.86, p < 0.05). There was no significant difference in the correlation between the selection ratio by the T0 netting, T45 netting, and T90 netting with a 70% hanging ratio (one-way ANOVA, p > 0.05). The results of this study showed that selectivity such as T45 netting and T90 netting appeared when the hanging ratio, which maximizes the area of T0 netting, was maintained at 70%.

• 한국어류학회지
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• 제30권2호
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• pp.75-83
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• 2018

이 연구는 우리나라 고유종인 낙동납자루의 초기생활사를 연구하여 분류학적 연구의 기초자료로 이용하고자 한다. 낙동납자루는 수컷의 뒷지느러미 가장자리 검은색 띠가 짙고, 암컷의 산란관이 오렌지색을 띠는 것으로 알려져 있다. 난의 형태는 방추형이었고, 난경은(장경${\times}$단경) $4.41{\times}1.44mm$였다. 부화에 소요되는 시간은 수온 $21.0^{\circ}C$일 때 126시간이 소요되었다. 부화 직후의 자어는 난황을 가지고 있으며, 평균 전장은 $5.91{\pm}0.18mm$ (n=5)였다. 부화 후 18일째에는 꼬리지느러미가 발달하였고, 평균 전장은 $8.02{\pm}0.08mm$ (n=5)였다. 부화 후 41일째 자어는 난황을 대부분 흡수하였고, 평균 전장은 $8.70{\pm}0.23mm$ (n=5)였다. 부화 후 80일째 자어는 지느러미 기조 수가 정수에 달하였고, 평균 전장 $12.6{\pm}0.28mm$ (n=5)였다. 지느러미 기조 수는 등지느러미 ⅲ.8개, 뒷지느러미 ⅲ. 9~10개, 꼬리지느러미 19개, 측선비늘 수 32~35개로 어미와 유사하였다.